巻号一覧
28 巻, 10 号
選択された号の論文の14件中1～14を表示しています
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Part III. General Properties of Nucleoside PhosphotransferaseKoji MITSUGI1964 年 28 巻 10 号 p. 659-668
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリー1) Nucleoside phosphotransferase activity was observed somewhere in the bacterial cells, as well as a relatively large amount of 5'-nucleotidase and of phosphomonoesterase.
2) Nucleoside phosphotransferase activity and its product specificity did not depend upon the cultural conditions or cultural period but were suggested to be due to the inherent or basic character of strains.
3) Aromatic phosphate compounds and nucleotides are useful for this reaction as donor; especially in the latter case, product specificity of the reaction was confirmed to correlate with nucleotide isomer added as donor, namely with donor specificity.
4) The phosphate transfer reaction using nucleotide as donor was reversible, but that using p-NPP was irreversible.
5) The synthesis and accumulation of IMP was apparent to correlate with the concentration of donor phosphate rather than that of p-NP and Pi liberated.
6) The yield of 5'-IMP from HxR and p-NPPwas amounted to about 70_??_80%, this reaction, therefore, is useful for the preparation or production of 5'-IMP.抄録全体を表示PDF形式でダウンロード (644K) -
Part IV Effects of Inhibitors and Metallic Ions on the Nucleoside PhosphotransferaseKoji MITSUGI1964 年 28 巻 10 号 p. 669-677
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリー1) Few reagents known so far as the inhibitors of acid phosphatase inhibited the nucleoside phosphotransferase of bacteria at pH 4.0.
2) Cu++ or Zn++ stimulated markedly the synthesis of nucleotide and shifted the optimum pH of nucleoside phosphotransferase from 5.0 to 4.0.
3) Cu++ and Zn++ inhibited the formation of hypoxanthine from inosine or 5'-inosinic acid.
4) The yield of 5'-inosinic acid synthesize amounted to about 80%, in this optimum condition, which corresponds to about twofold increase against the value obtained without metallic ion.
5) The effect of Cu++ or Zn++ did not seem to correlate with the permeability of cell membrane, or with the nitro radical at para-position of phenylphosphate added as donor or substrate.
6) The roles of Cu++ and Zn++ on phosphomonoesterase and 5'-nucleotidase activities and on the protecting ability from acidic inactivation of these enzyme activities were not always the same. The nonidentity of the so-called phosphatase and nucleoside phosphotransferase was discussedfrom these results.抄録全体を表示PDF形式でダウンロード (541K) -
Yoshiaki ODA, Kuniaki KOSHIBA1964 年 28 巻 10 号 p. 678-685
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーThis paper presents the steric configuration of a polysaccharide obtained from the latex of the Japanese lacquer tree. Thus, the polysaccharide consisted of galactose and galacturonic acid, and the main chain of polysaccharide was proved to be linked with 1→3 linkage of galactose residue of 6-O-galacturonosyl galactose unit by the periodate oxidation method. The molecular weight was calculated to be 6×104 by Archibald's ultracentrifugal method. The configuration of the polysaccharide was discussed to be a helical structure of which one turn of the helices consists of 6 to 7 6-O-galacturonosyl galactose units and the diameter of the helix is 21Å and one pitch is 9.5Å on the basis of viscosity measurements, color reaction with iodine, crystallization with capronic acid and consideration of molecular models.抄録全体を表示PDF形式でダウンロード (2255K) -
Kazuyuki MAEKAWA, Masahisa KUSHIFE1964 年 28 巻 10 号 p. 686-689
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーCrude RNA obtained by the decoction of destroyed yeast cells showed some immunochemical reaction with the antisera, but had little or no immunizing ability.
Crude RNA treated with phenol and reprecipitated by ethanol, and pure RNA obtained from phenol-treated RNA had the immunizing ability. It seems, therefore, that the treatment with phenol gave RNA some conformational change and led to acquirment of the immunizing ability. Its ability to immunize rabbits is weak and the antibody produced by it is a non-precipitating, and an incomplete one.抄録全体を表示PDF形式でダウンロード (259K) -
Part VI. Accumulation of 5'-Xanthylic Acid by Guanine-Requiring Mutants of Micrococcus glutamicus I. Isolation and Characterization of 5'-Xanthylic AcidMasanaru MISAWA, Takashi NARA, Kiyoshi UDAGAWA, Shigeo ABE, Shukuo KIN ...1964 年 28 巻 10 号 p. 690-693
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーIt was found that a guanine and adenine-requiring mutant of Micrococcus glutamicus accumulated the ultraviolet-absorbing substance in the culture fluid. A KY9978 strain, which accumulated the largest amount of the substance, we selected from the guanine auxotrophs derived from the guanine-adenine doubleless mutant. The substance was isolated in a crystalline form from the culture fluid by the use of ion exchange resins, Diaion SA 21A and SK No. 1, and identified chemically and enzymatically as 5'-xanthylic acid, an intermediate from 5'-inosinic acid to 5'-guanylic acid on the purine nucleotide biosynthesis.抄録全体を表示PDF形式でダウンロード (251K) -
Part VI. Accumulation of 5'-Xanthylic Acid by Guanine-Requiring Mutants of Micrococcus glutamicus II. Studies on Cultural ConditionsMasanaru MISAWA, Takashi NARA, Shukuo KINOSHITA1964 年 28 巻 10 号 p. 694-699
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーA guanineless mutant of Micrococcus glutamicus, KY9978 strain, which was derived from a guanine-adenine doubleless mutant, KY9938 strain, was able to grow on guanine more abundantly than the corresponding nucleoside and nucleotide, but unable to grow on 2, 6-diaminopurine. A fermentation medium most suitable for XMP production by KY9978 strain was decided by varying the concentrations and kinds of ingredients in the medium. Of particular importance in media is the concentration of guanine, the excessive addition of which decreased XMP yields remarkably. This might suggest negative feed back mechanisms involved in GMP biosynthesis by this strain. Typical chemical changes in XMP fermentation showed that the maximal amount of the product was approximately 2.75mg per ml of medium.抄録全体を表示PDF形式でダウンロード (360K) -
Tomoyuki ISHIKURA, Tadashi SAKAMOTO, Ichiya KAWASAKI, Toshinao TSUNODA ...1964 年 28 巻 10 号 p. 700-709
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーTo shorten the time necessary for the determination of L-glutamic acid a turbidimetric microbiological assay method with Lactobacillus arabinosus 17-5 has been studied. It was found that the assay can be completed within 30 hours including the incubation period for the inoculum, the assay and the time for medium preparation. Turbidimetric assay methods of other amino acids such as DL-alanine with Leuconostoc citrovorum 8081, L-aspartic acid and L-lysine with Leuc. mesenteroides P-60 have also been studied.抄録全体を表示PDF形式でダウンロード (488K) -
Un analogue de la pénicillamine et sa thiolactonePar Masanao MATSUI, Motohiro NISHIO1964 年 28 巻 10 号 p. 710-713
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリー2-Methyl-3-benzylmercaptopropanal, obtained by condensation of methacrolein with benzyimercaptan, was treated with a mixture of sodium cyanide, ammonium carbonate and triethylamine to give two diastereomeric hydantoins. Each diastereomer gave the same S-benzylmercapto-valine on treatment with alkali, which shows that the epimerization occured in this condition and the more stable one was formed. This compound was treated with sodium in liquid ammonia to give the corresponding disulfide. S-benzylmercapto-valine gave 2-amino-3-methyl-butyrothiolactone when refluxed under inert atmosphere after the reduction. These compounds were derived to 2-methyl-homocysteic acid by treatment with bromine and valine by treatment with Raney nickel.抄録全体を表示PDF形式でダウンロード (225K) -
Part XI. Purification and Properties of L-Glutamic Acid Dehydrogenase from Micrococcus glutamicusKazuo OSHIMA, Katsunobu TANAKA, Shukuo KINOSHITA1964 年 28 巻 10 号 p. 714-722
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーMicrococcus glutamicus, a glutamate-producing bacterium, is known to have strong activity of L-glutamic acid dehydrogenase which requires NADP as co-enzyme. In this paper, the NADP-specific L-glutamic acid dehydrogenase was purified from M. glutamicus by means of heat treatment with sodium sulfate, precipitation with acetic acid and diethyl-amino-ethyl (DEAE) cellulose column chromatography. The activity of the purified enzyme preparation reached 200-fold as high as that of the crude extract. Some properties of the purified enzyme were investigated. As a result, it was found that the highly purified enzyme preparation acted not only on L-glutamic acid (L-GA) but also on α, ε-diaminopimelic acid (α, ε-DAP) in the presence of NADP. Some of the probable consideration for the dehydrogenation of L-GA and α, ε-DAP are noted.抄録全体を表示PDF形式でダウンロード (505K) -
Part VI. Catalytic Hydrogenation of Kojic AcidItsuo ICHIMOTO, Chuji TATSUMI1964 年 28 巻 10 号 p. 723-727
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーThe catalytic hydrogenation of kojic acid in the presence of Raney nickel and noble metals such as platinum or palladium was studied. The hydrogenated products were isolated and identified. By using Raney nickel catalyst, hexahydro kojic acid was obtained in good yield. Allo-maltol, hexahydro kojic acid and hexahydro allo-maltol were produced over platinum catalyst. Also, allo-maltol, tetrahydro kojic acid and tetrahydro all maltol were produced over palladium catalyst. The success of the direct conversion from kojic acid to allo-maltol may be emphasized.抄録全体を表示PDF形式でダウンロード (355K) -
Nobumaro KAWASHIMA, Yasuo TAMAOKI, Ikuzo URITANI1964 年 28 巻 10 号 p. 728-733
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーComparison of protein content, phosphatase activity and peroxidase activity was made using extracts prepared with and without isoascorbate from sweet potato roots infected by the black rot pathogen. There was no noticeable difference between the two kinds of extracts. Immunological results indicated that some proteins were modified when isoascorbate was omitted from the extraction medium. After SEPHADEX chromatography, protein content and peroxidase activity did not change appreciably despite the presence or absence of isoascorbate. Acid phosphatase activity was strongly decreased in the absence of isoascorbate. It was concluded that acid phosphatase formed a complex with the oxidation products from polyphenols formed by polyphenol oxidase. SEPHADEX sieving was found to be a very useful method to separate proteins quickly from polyphenols, because the latter were strongly adsorbed on SEPHADEX.抄録全体を表示PDF形式でダウンロード (400K) -
A. G. JÖNSSON, S. M. MARTIN1964 年 28 巻 10 号 p. 734-739
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーThe five strains of A. fumigatus examined showed marked variation in their ability to produce protease in submerged culture. Also marked differences in protease production were observed when individual strains were tested in different media. Yields of up to 3.7 EUPH 10.0 per 1 were obtained in a liver-glucose medium with the one pathogenic strain included in the study.抄録全体を表示PDF形式でダウンロード (315K) -
NAD-linked D-Glucose-isomerizing and D-Mannose-isomerizing Enzyme from Paracolobacterum aerogenoidesYoshiyuki TAKASAKI, Osamu TANABE1964 年 28 巻 10 号 p. 740-741
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (125K) -
Shinji OKUMURA, Masao SHIBUYA, Shimpachi KONISHI, Moriyoshi ISHIDA, Te ...1964 年 28 巻 10 号 p. 742-743
発行日: 1964年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (89K)
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