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Volume 29, Issue 8
Displaying 1-17 of 17 articles from this issue
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Part VII. Effect of Water Level of Culture Medium on Mycelial Compositions and Enzyme ProductionTakeji MIZUNUMA1965 Volume 29 Issue 8 Pages 703-713
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSStudies were made on the effect of water level of culture medium on the mycelial com-positions and enzyme production in Aspergillus sojae K. S. The mold was grown on the media of various water levels made of powder of defatted soybean and wheat granule.
1. The mycelia grown on the medium of low water level consumed more oxygen and formed less ammonia per mg dry weight.
2. The above mycelia were richer in 2 N H2SO4-soluble glycogen, 60% H2SO4-soluble carbohydrates, and RNA than those grown on the high water medium during the whole period of incubation.
3. The mycelia grown on the high water medium had a tendency to accumulate AMP, ADP, inorganic phosphorus, fructose 6-phos-phate, ribose 5-phosphate and monophos-phoglycerate according to the lapse of culture time.
4. Production of protease and α-amylase was more remarkable in the mycelia grown on the low water medium than in those grown on the high water medium.View full abstractDownload PDF (647K) -
Part VIII. Biosynthesis of the Pyrrolidine Ring of Nornicotine in the Excised Root CultureShigenobu MIZUSAKI, Takuro KISAKI, Einosuke TAMAKI1965 Volume 29 Issue 8 Pages 714-718
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSDuplicate feeding experiments of dl-ornithine-2-14C to the excised tobacco root culture were made, and the radioactive nornicotine was isolated. Approximately two thirds of the radioactivity was located in the 2-position of the pyrrolidine of the nornicotine in these experiments. This fact indicates that there are two modes in nornicotine biosynthesis: exclusive incorporation to the C-2 and equal incorporation to C-2 and C-5 from C-2 of ornithine.
On the basis of this finding, biosynthetic route was discussed.View full abstractDownload PDF (1214K) -
Part IX. Question for Nornicotine as a Precursor of NicotineShigenobu MIZUSAKI, Takuro KISAKI, Einosuke TAMAKI1965 Volume 29 Issue 8 Pages 719-723
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSdl-Ornithine-2-14C, dl-methionine-14CH3 and partially racemized l-nornicotine-2, 5-14C, were administered to aseptically grown excised roots (N. rustica var. Brasilia). Incorporation of their radioactivity to nicotine was compared. The extent of their radioactive incorpo-ration to nicotine was high in the order of ornithine, methionine and nornicotine; incorpo-ration of radioactivity of nornicotine to nicotine was extraordinarily low. 15N-Labeled nornicotine was also fed to the same materials and 15N distribution was examined. Most of 15N still remained in the nornicotine reisolated. Marked amounts of 15N were located in the ethanol-insoluble fraction, the amino acid fraction and the substances having chro-matographic RF value close to that of nicotine. Only small amount of 15N was incorpo-rated to the isolated nicotine.
Nornicotine is generally accepted to be a direct precursor of nicotine in tobacco plants. From these findings, however, it can be said that the biosynthesis of nicotine can occur through other routes without going through nornicotine.View full abstractDownload PDF (928K) -
Koki HORIKOSHI, Yoichi OHTAKA, Yonosuke IKEDA1965 Volume 29 Issue 8 Pages 724-727
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSRibosomes were isolated from dormant and germinating conidia of Asp. oryzae No. 13. The ribosomes which consisted of 80 S were easily dissociated into 40 S and 60 S in low Mg++ buffer. Polyribosomes were not found in dormant conidia, but were found in germinating conidia. Ribosomes in Aspergillus fungi consisted of almost equal amount of RNA and protein, and the base compositions of RNA were alike, as compared as ribo-somal RNA between dormant and germinating conidia.View full abstractDownload PDF (1345K) -
Part I. The Preparative MethodTeiichiro ITO, Tadao ISHII, Motohiro NISHIO1965 Volume 29 Issue 8 Pages 728-731
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSα-Methylthio-cinnamic acid and its substituted analogues (III) were synthesized from their respective β-aryl-α-thiopyruvic acids (II). In connection with the study on the tauto-meric ene-thiol structure of β-aryl-a-thiopyruvic acids (II), 4-arylidene-l, 3-oxathiolan-5-one (IV) were prepared from compounds II.View full abstractDownload PDF (219K) -
2e communication Stéréochimie et Les Spectres RMNMotohiro HISHIO, Teiichiro ITO1965 Volume 29 Issue 8 Pages 732-736
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSAn improved method of preparation and the separation of geometrical isomers of cis and trans α-methylthio-cinnamic acid derivatives are described. The observation on the NMR spectra and the determination of geometrical structure of these compounds by various spectrometric methods are also included.View full abstractDownload PDF (267K) -
Part I. Separation and Determination of Nucleosides and Bases by Electrophoretic MethodTakuo SAKAI, Tatsurokuro TOCHIKURA, Koichi OGATA1965 Volume 29 Issue 8 Pages 737-741
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSIn this study, the authors developed a simplified method for the separation and the quantitative determination of nucleosides and bases, using paper electraophoretic technique. By this method, nucleosides and bases were well separated and determined in a fairly short time. Thus, this method was expected to be as accurate as the published methods and was believed to be a better method for both quantitative determination and detection of the nucleosides and bases in a large number of samples.View full abstractDownload PDF (728K) -
Part II. Studies on Nucleoside-N-Ribosyl Group Transfer Reaction in Bacteria (l)Takuo SAKAI, Tatsurokuro TOCHIKURA, Koichi OGATA1965 Volume 29 Issue 8 Pages 742-750
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSThe bacterial enzymes which catalyze the nucleoside-N-ribosyl group transfer reaction between pyrimidine and purine nucleoside were studied. It was found that some bacteria had the intracellular enzyme system which catalyzes the nucleoside-N-ribosyl group transfer and could produce purine nucleosides from purine bases and pyrimidine nucleosides. Using the cell-free extract of Erwinia carotovora, inosine was isolated in fine crystals from uridine and hypoxanthine through the nucleoside-N-ribosyl transfer reaction.
It seemed that the bacteria which can transfer the N-ribosyl group may be divided into two groups, based on the pH dependency of this reaction. This reaction was stimu-lated by inorganic phosphate.View full abstractDownload PDF (1642K) -
Hisateru MITSUDA, Kyoden YASUMOTO, Kazumasa YOKOYAMA1965 Volume 29 Issue 8 Pages 751-756
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSA relatively stable free radical signal was detected by ESR spectroscopy in the mela-noidin prepared from glycine and glucose. All attempts made to remove molecular oxygen from the melanoidin matrix resulted in a reversible increase in signal height without changing the line width and g-value of central resonance. Nitric oxide, n well-known quenching agent for radicals, retarded the reaction ensuing in disappearance of furfurals and deposition of a new compound. These results allowed to envisage a steps of free radical formation involved in the amino-carbonyl reaction and also nature of the free radical species.View full abstractDownload PDF (1129K) -
Tokuya HARADA, Tadashi YOSHIMURA, Hidemasa HIDAKA, Atsuo KOREEDA1965 Volume 29 Issue 8 Pages 757-762
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSA slimy non-spore-forming bacterium strain 10C3 isolated from soil was motile with peritrichous flagella and named Alcaligenes faecalis var. myxogenes. Studies were made on the conditions necessary for maximal production of a new acidic succinoglucan polysaccha-ride by this strain in shaken cultures. Much production was observed with sucrose, glucose, xylose, galactose, cellobiose, maltose, fructose, mannose and rhamnose. The yield was greatest with sucrose and decreased in order with the above sugars from about 36 to 23 per cent. The most suitable medium contained 4 per cent sugar, 0.5 per cent yeast extract and one per cent calcium carbonate in tap water. The optimum temperature was 28°C.View full abstractDownload PDF (1002K) -
Hiroshi HYODO, Shoji BANDO1965 Volume 29 Issue 8 Pages 763-768
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSA polyphenol oxidase (o-diphenol oxidase) [o-diphenol: O2 oxidoreductase E. C. 1.10. 3.1] from sweet potato named component IIb was highly purified. The copper content of this enzyme was measured by neutron activation analysis. Samples were analyzed with or without chemical separation after neutron irradiation. The copper content of the enzyme was determined to be 0.27%, and the minimum molecular weight of this enzyme was caluculated to be 23, 500.View full abstractDownload PDF (358K) -
K. M. L. AGRAWAL1965 Volume 29 Issue 8 Pages 769-773
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSSterilized aqueous mixtures containing para-formaldehyde, and Ni**, Co++, V++ ions as catalysts were exposed to sunlight for 400 hours. Similar mixtures were covered with four folds of black cloth and were kept near the exposed mixtures. The catalytic effect of these metallic ions on photochemical fixation of nitrogen was found in the following order:
vanadium**>cobalt**>nickel**
Synthesis of amino acids and peptides were also observed in the mixtures containing these catalysts on exposure to sunlight. However, mixtures kept in dark indicated the formation of traces of aminoacids only.View full abstractDownload PDF (305K) -
Koichi SHIMBAYASHI, Yu IDE, Toshio YONEMURA1965 Volume 29 Issue 8 Pages 774-778
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSThe concentration of amino acids in the plasma from female sheep was determined. The plasma amino acid pattern was very similar to that of cow which was investigated in authors' laboratory. The concentration of glycine was considerably higher than that of alanine; this is the reverse of the pattern in man and cat. When the animal was fed on the severe low DCP diet, the concentration of glycine in the plasma was higher than that in the normal feeding. The concentration of amino acids among the portal, jugular vein, and carotid artery was compared.View full abstractDownload PDF (324K) -
Makoto KANDATSU, Masaaki HORIGUCHI, Masato TAMARI1965 Volume 29 Issue 8 Pages 779-780
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSDownload PDF (133K) -
Makoto KANDATSU, Masaaki HORIGUCHI1965 Volume 29 Issue 8 Pages 781-782
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSDownload PDF (128K) -
Yoshio NOZAKI, Mikao MAYAMA, Koji AKAKI, Daisuke SATOH1965 Volume 29 Issue 8 Pages 783
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSDownload PDF (86K) -
Masanao MATSUI, Hirosuke YOSHIOKA, Yasuhiro YAMADA, Hideo SAKAMOTO, Ta ...1965 Volume 29 Issue 8 Pages 784-786
Published: 1965
Released on J-STAGE: November 27, 2008
JOURNAL FREE ACCESSDownload PDF (223K)
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