巻号一覧
32 巻, 8 号
選択された号の論文の26件中1～26を表示しています
- |<
- <
- 1
- >
- >|
-
Part I. Screening of α-Glucosidase from Plant Sources and Changes of α-Glucosidase Activity during the Germination Stages of BuckwheatMiho TAKAHASHI, Tokuji SHIMOMURA1968 年 32 巻 8 号 p. 923-928
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPresent investigation deals with a search for plant sources which have high α-glucosidase activities. As a result of the screening test on 8 kinds of plant source (spring wheat, maize, buckwheat, malted barley, oat, soybean, azuki bean and kidney bean), buckwheat seed was found to have the highest α-glucosidase (maltase) activity. Changes in α-glucosidase and amylase activities during the germination stages of buckwheat were also examined. It was found that α-glucosidase was rich in seeds and seedlings at initial stages of germination. In addition, the probable existence of glucamylase activity was suggested in the former, and a rapid appearance of α-amylase was recognized in the latter.
It was concluded that ungerminated buckwheat seed was excellent as the material for the preparation of plant α-glucosidase.抄録全体を表示PDF形式でダウンロード (366K) -
Part II. Purification of Buckwheat α-Glucosidase and Some Properties on Maltose and Soluble StarchMiho TAKAHASHI, Tokuji SHIMOMURA1968 年 32 巻 8 号 p. 929-939
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーBuckwheat a-glucosidase was purified approximately 300-fold by CM-cellulose treat-ment, calcium phosphate gel treatment, fractionation with ammonium sulfate, chromato-graphy on a column of calcium phosphate gel and CM-Sephadex treatment. The purified a-glucosidase showed one peak ultracentrifugally but was heterogeneous in moving-boundary electrophoresis.
The enzyme hydrolyzed not only maltose but also soluble starch, and glucose was the sole product from both substrates. At a lower substrate concentration (0.5%), the rate of the initial reaction with maltose was about 3 times higher than that with soluble starch. At higher substrate concentrations (2.5% and 5%) the enzyme showed a trans-glucosylase action toward both substrates.
The following properties were perfectly identical on maltose and soluble starch: optimum pH 5.0; optimum temperature, ca. 52°C; range of pH stability, 4._??_7.3; temperature of stabili-ty, 45°C for 10 min; enzymatic activity on each substrate, almost lost at 60°C for 30 min.抄録全体を表示PDF形式でダウンロード (2138K) -
Part IV Inhibition of the Respiration in YeastKiyoshi HARADA, Ryoichi HIGUCHI, Isamu UTSUMI1968 年 32 巻 8 号 p. 940-946
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーSorbate inhibited the growth of baker's yeast at the logarithmic and stationary phases and its inhibition showed the Type II mode proposed by Tamiya et al.
Microscopic observation of the yeast cells during growth demonstrated that the sorbate inhibition was fungistatic, but not fungicidal. The respiration of the yeast was mano-metrically determined and the mechanism of the inhibition was suggested that sorbate would competitively combine with coenzyme A and acetate and would consequently inhibit the enzyme reaction relating coenzyme A. In addition, it was clarified that sorbyl-coenzyme A was also determined by the method of the enzymatic acetylation of sulfani-lamide. This would suggest that the sorbyl moiety might be transferred to 4-amino radical of sulfanilamide enzymatically as well as in the case of acetyl-coenzyme A.抄録全体を表示PDF形式でダウンロード (447K) -
Mitsuo ISHIDA1968 年 32 巻 8 号 p. 947-955
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーIt was demonstrated by gel filtration that the enzymes from house flies for the me-tabolisms of α- and r-1, 2, 3, 4, 5, 6-hexachlorocyclohexane, r- and δ-2, 3, 4, 5, 6-pentachloro-cyclohex-1-ene, 2, 3, 4, 5, 6-pentachlorobenzyl chloride, 1-chloro-2, 4-dinitrobenzene, 2, 3, 4, 5, 6-pentachloro-β-nitrostyrene and 1, 1, 1-trichloro-2, 2-di-(p-chlorophenyI) ethane were indistin-gishable from one another in their molecular sizes which were estimated at 36, 000 to 38, 000 by using reference proteins. The same result was obtained with rat liver homogenate. Two house fly isozymes were separated by CM-cellulose column chromatography. The house fly enzymes were clearly separated from rat liver enzymes by electrophoresis, which also revealed the presence of 2 isozymes in the rat liver homogenate.抄録全体を表示PDF形式でダウンロード (561K) -
Part XVI. Accumulation of 5'-Inosinic Acid and 5'-Uridylic Acid by Brevibacterium insectiphiliumTakashi NARA, Isao KAWAMOTO, Masanaru MISAWA, Shukuo KINOSHITA1968 年 32 巻 8 号 p. 956-962
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーBrevibacterium insectiphilium KY 3446 (Steinhous, Breed AHU 1401) was found to ac-cumulate IMP from hypoxanthine and UMP from uracil, respectively. This strain is thus considered to present the fourth example in salvage-type fermentation, in addition to Micrococcus s_??_donensis, Arthrobacter citreus and Brevibacterium ammoniagenes reported previously.
IMP from adenine and UMP from cytosine were also produced by KY 3446, re-spectively. Further, the addition of inosine and adenosine instead of the bases also caused IMP accumulation.
This strain grew well on sucrose medium, and produced IMP and UMP in higher yields on sucrose than on glucose medium.
Excessive amounts of Mn2+ stimulated growth, but markedly inhibited IMP production. The optimal concentration of Mn2+ for IMP accumulation induced morphogenetic alterations from normal and small to abnormal and large cells.抄録全体を表示PDF形式でダウンロード (1110K) -
Part III. Activation of Intracellular RibonucleaseTakeshi UOZUMI, Gakuzo TAMURA, Kei ARIMA1968 年 32 巻 8 号 p. 963-968
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーIn was found that an intracellular ribonuclease was present as an inactive form in the fresh mycelium of Asp. oryzae. It was about 3 times activated either by 3M urea or by the autolysis of mycelium at 30°C for 20hr. The optimum pH of the ribonuclease activity was 8.3. It was heat sensitive (60°C, 10min), and completely inhibited by 5mM EDTA. It was activated by 1 MM Mg2+ and inhibited by Zn2+, Ca2+, Cd2+, Co2+ and Cu2+.抄録全体を表示PDF形式でダウンロード (331K) -
Part IV Presence of a Ribonuclease Inhibitor in the MyceliaTakeshi UOZUMI, Gakuzo TAMURA, Kei ARIMA1968 年 32 巻 8 号 p. 969-974
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーA ribonuclease inhibitor was found in the soluble fraction of fresh mycelium of Aspergillus oryzae. The inhibitor was nondialyzable, insensitive to heating at 80°C for 10 min, and salted out by 0.4 to 0.7 saturation of ammonium sulfate. The inhibitor disappeared with the 3.1 times increase of intracellular ribonuclease activity in the course of autolysis of mycelium at 30°C for 24hr. The inhibitor obtained from fresh mycelium inactivated the ribonuclease on the cell walls, in the large particles and soluble fraction of the autolyzed mycelium.抄録全体を表示PDF形式でダウンロード (327K) -
Part II. Purification and Properties of a Proteolytic Enzyme, Cathepsin D, from Rabbit MuscleAtsushi SUZUKI, Masao FUJIMAKI1968 年 32 巻 8 号 p. 975-982
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPresence of high cathepsin D activity in the aqueous extract of the acetone-dried powder from rabbit muscle (L. dorsi) was observed. By fractionation with acetone and by DEAE-Sephadex chromatography, electrophoretically homogenous enzyme with the specific activity of 12, 620 was isolated, and this value was 3600 times of the original sarcoplasmic cathepsin D activity.
Its activity to hydrolyze denatured hemoglobin was maximal at pH 4.0 and at 40_??_47°C. Slight inhibition by zinc and manganese ions (0.01M) was observed. No enhance-ment of the activity by any metal ions and sulfhydryl compounds examined was observed.
This enzyme was stable at fairly acidic pH, and the loss of its activity, incubated for 10 min at pH 3.0, was about 50% and 90%, at 60°C and 70°C, respectively.
The purified cathepsin D in this report showed similar properties as sarcoplasmic cathepsin, and cathepsin D from spleen. However, the present purified cathepsin D was different from the latter cathepsin in the stability at acidic pH.抄録全体を表示PDF形式でダウンロード (1259K) -
Part II. Formation of 4 (5)-Ethylimidazole from the Reaction of L-Rhamnose and AmmoniaMasahiko KOMOTO, Hironobu TSUCHIDA1968 年 32 巻 8 号 p. 983-987
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーReducing sugars react in aqueous ammonia to form several imidazolic compounds. An unknown compound was newly isolated from the reaction mixture of L-rhamnose and ammonia, and identified as 4 (5)-ethylimidazole. It was found that this compound was formed in aqueous ammonia only from such reducing sugars as rhamnose, fucose etc. containing a terminal methyl group. Consequently, it was inferred that in aqueous ammonia L-rhamnose decomposes to form ethylglyoxal as an intermediate of 4 (5)-ethylimidazole.抄録全体を表示PDF形式でダウンロード (306K) -
Kazuya HAYASHI, Danji FUKUSHIMA, Koya MOGI1968 年 32 巻 8 号 p. 988-994
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーThe molecular conformation of the alkaline proteinase of Aspergillus sojae in aqueous solution was investigated by the optical rotatory dispersion, Cotton effects, infra-red absorption spectra (amide I and V bands), ultraviolet difference spectra, etc. It is concluded that; (1) there are about 10 to 15% of the α-helix and a small amount of the β-structure in the enzyme molecule, but most parts of the peptide chain are present as the disordered structure; (2) the enzyme molecule is compactly folded even in the disordered parts; and (3) the two tryptophan residues involved in the peptide chain are burried in the interior of the molecule.抄録全体を表示PDF形式でダウンロード (443K) -
Part III. A Synthesis of DesacetamidocolchiceineShin-ichi KANEKO, Masanao MATSUI1968 年 32 巻 8 号 p. 995-1001
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPschorr reaction of 4-[3-(3, 4, 5-trimethoxyphenyl)-propyl]-5-amino-tropolone (VII) gave desacetamidocolchiceine (IIa), though the oxidative coupling reaction of 4-[3-(3, 4-dihydroxy-5-methoxy phenyl)-propyl]-tropolone (Xa) failed to give the same compound (IIa).抄録全体を表示PDF形式でダウンロード (416K) -
Part VI Synthesis of Cyclitol α-D-MannosidesHisao SHIBATA, Daikichi NISHIMURA, Norio KURIHARA, Minoru NAKAJIMA1968 年 32 巻 8 号 p. 1002-1005
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーFrom 2, 3, 4, 6-tetra-O-benzoyl-a-D-mannopyranosyl bromide by the Konigs-Knorr reac-tion, α-A-mannosides of (+)- and (-)-N-carbobenzoxy-trans-2-amino-cyclohexanol and 3-bromo-3-deoxy-1, 2;4, 5-di-O-isopropylidene-muco-inositol were synthesized.抄録全体を表示PDF形式でダウンロード (275K) -
Part VII Conversion of Methyl 3-Amino-3-deoxy-α-D-mannopyranoside to Methyl 3-Amino-3-deoxy-α-D-glucopyranosideHisao SHIBATA, Isao TAKESHITA, Norio KURIHARA, Minoru NAKAJIMA1968 年 32 巻 8 号 p. 1006-1009
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーAs an approach to the synthesis of α-D-glucopyranosides, the conversion of α-D-man-nopyranosides to α-D-glucopyranoside was studied. Methyl 3-benzamido-4, 6-O-benzylidene-3-deoxy-α-D-mannopyranoside (II) was oxidized to methyl 3-benzamido-4, 6-O-benzylidene-3-deoxy-α-D-arabino-hexosid-2-ulose (III). Reduction of III gave methyl 3-benzamido-4, 6-O-benzylidene-3-deoxy-α-D-glucopyranoside (IVa).抄録全体を表示PDF形式でダウンロード (249K) -
Part III. Synthesis of 4-Amino-5-cyano-6-methylmercaptopyrrolo-[2, 3-d] pyrimidine-7-β-D-ribosides (Toyocamycin Analogs)Hajime IWAMURA, Takeshi HASHIZUME1968 年 32 巻 8 号 p. 1010-1015
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーBoth ribofuranoside (3) and ribopyranoside (5) of 4-amino-5-cyano-6-methylmercaptopyr-rolo[2, 3-d]pyrimidine (analogs of toyocamycin) have been synthesized for the first time by the fusion procedure using bis-(p-nitrophenyl) hydrogen phosphate as catalyst. Evidence for the β-configuration has been provided by analyzing the NMR spectra and ORD curves. The tumor-inhibitory activity of 3 against Ehrlich ascites carcinoma in mice has also been examined.抄録全体を表示PDF形式でダウンロード (411K) -
Part I. Some Lactones of 5-Substituted-5-hydroxy-2-enoic Acids as a Synthetic Butter or Butter Cake FlavorAkio NOBUHARA1968 年 32 巻 8 号 p. 1016-1020
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーHomologous unsaturated a-lactones were prepared and the nature of their flavors was investigated. Among those, lactones of 5-hydroxy-2-decenoic acid and 5-hydroxy-8-methyl-2-nonenoic acid were shown to have a flavor the most similar to a butter or butter cake flavor.抄録全体を表示PDF形式でダウンロード (291K) -
Part I. Effect of Showdomycin on the Syntheses of Nucleic Acids and Proteins in Escherichia coli K-12Yoshihide KOMATSU, Kentaro TANAKA1968 年 32 巻 8 号 p. 1021-1027
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーShowdomycin, 2-β-D-ribofuranosylmaleimide, inhibited the incorporation of amino acids and purine and pyrimidine bases into macromolecules in E. coli K-12 cells at low concentrations. The inhibitory action of showdomycin could be reversed by the addition of a nucleoside or a sulfhydryl compound. In marked contrast to common nucleosides, the pseudouridine showed no such effect. This may indicate that the N-glycosyl linkage in the nucleoside is a structural requirement for its reversing activity on the inhibitory action of showdomycin.
N-Ethylmaleimide, which has structural similarity to showdomycin, inhibited the in-corporation of amino acids and purine and pyrimidine bases as well as showdomycin. The inhibitory action of N-ethylmaleimide, however, was not reversed by the addition of a nucleoside. This may indicate that there may be difference in the mechanism of the inhibitory action between N-ethylmaleimide and showdomycin.抄録全体を表示PDF形式でダウンロード (383K) -
Part III. On the Structure of Flavoyamenin, a New C-Glycosyl Flavone in the Petals of Iris Nertshinskia Loddiges form. alubiflora HONDAAsa KAWASE1968 年 32 巻 8 号 p. 1028-1032
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーFlavoayamenin (I), C28H32O15, mp 258_??_9°C, a new C-glycosyl flavone isolated from ethanolic extracts of the petals of Iris Nertshinskia Loddiges form. alubifiora Honda (Iridaceae), has now been formulated as genkwanin-6-C-β-D-glucopyranosyl-x''-O-mono-D-glucoside. Basides, two other C-glycosyl flavones have also been isolated from the same extract by chromatography on a Nylon column, and identified as swertisin and swertia-japonin, respectively.抄録全体を表示PDF形式でダウンロード (293K) -
Part I. Oxidation of p-Xylene and Toluene by Cell-Free Enzyme Preparations of Pseudomonas aeruginosaJunichiro NOZAKA, Masamichi KUSUNOSE1968 年 32 巻 8 号 p. 1033-1039
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーThe crude extract obtained from Pseudomonas aeruginosa grown on xylene as sole carbon source converted p-xylene-methyl-14C or toluene-methyl-14C to p-toluic or benzoic acid, respectively. However, addition of p-methylbenzyl or benzyl alcohol to the reaction mixture resulted in accumulation of p-methylbenzyl or benzyl alcohol. This indicates that in the crude extract, p-xylene or toluene is metabolized via its corresponding alcohol to p-toluic or benzoic acid, respectively. The enzyme system responsible for these reactions required NAD or NADH and FAD, and could be stabilized by the presence of glutathione. When the crude extract was fractionated by the use of DEAE-cellulose chromatography, it was demonstrated that two distinct protein fractions and two cofactors (NADH and FAD) were required for the step of the hydroxylation of p-xylene or toluene to its corresponding alcohol. NAD, NADP or NADPH had very few or little activity. FMN partially replaced FAD.抄録全体を表示PDF形式でダウンロード (357K) -
Part II. Stereochemistry of 4-[3-(3, 4, 5-Trimethoxy-phenyl)-2-propenyl]-tropoloneShin-ichi KANEKO, Masanao MATSUI1968 年 32 巻 8 号 p. 1040-1043
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (132K) -
Masanao MATSUI, Goro YABUTA1968 年 32 巻 8 号 p. 1044-1045
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (108K) -
Kenichi ASAHI, Tosio SAKURAI, Kiyoshi ISONO, Saburo SUZUKI1968 年 32 巻 8 号 p. 1046-1047
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (82K) -
Juhyun YU, Gakuzo TAMURA, Kei ARIMA1968 年 32 巻 8 号 p. 1048-1050
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (133K) -
Juhyun Yu, Shinjiro IWASAKI, Gakuzo TAMURA, Kei ARIMA1968 年 32 巻 8 号 p. 1051-1053
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (736K) -
Haruoka OHASHI, Michio MATSUHASHI, Bunji MARUO1968 年 32 巻 8 号 p. 1054-1055
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (117K) -
Morifura ETO, Hideo OHKAWA, Ken KOBAYASHI, Toshiharu HOSOI1968 年 32 巻 8 号 p. 1056-1058
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (151K) -
Yoshikatsu MUROOKA, Tokuya HARADA1968 年 32 巻 8 号 p. 1059-1060
発行日: 1968年
公開日: 2008/11/27
ジャーナル フリーPDF形式でダウンロード (139K)
- |<
- <
- 1
- >
- >|