Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
36 巻, 8 号
選択された号の論文の25件中1~25を表示しています
  • Keiichiro MURAMATSU, Hisanao TAKEUCHI, Yasuo FUNAKI, Aiko CHISUWA
    1972 年 36 巻 8 号 p. 1269-1276
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    The influence of protein quality on the growth-depressing effect of excessive amount of 12 individual essential and semiessential amino acids was examined. Growing rats were fed for 3 weeks diets containing either 10.5% egg albumin or 11.6%, wheat gluten (equivalent to the protein content of a 10% casein diet) supplemented with 5% of each of the L-amino acids. In general, the pattern of growth depression produced by the addition of excess amino acids to the egg albumin or the wheat gluten diet was similar to that of the case of casein diet obtained previously under the same experimental conditions. however, the extent of these effects was dependent not only upon the kind of amino acid supplemented with but also upon the source of protein used, and the depressing effect of each of excess amino acids added to the wheat gluten diet was usually severer than those added to casein and egg albumin diets. No evidence was noted of any striking changes in the liver protein and nucleic acid concentrations by either diets, but total liver protein, RNA and DNA contents were decreased in some amino acid groups of the egg albumin diet and in all amino acid groups of the wheat gluten diet except the lysine addition. The free amino acid level in plasma generally showed extreme elevation for the amino acid supplemented in excess in the diet, and in most cases the extent of the elevation was correlated with the growth depression.
  • Atsushi MURANO, Tadashi DOI, Hagimu KITAHARA
    1972 年 36 巻 8 号 p. 1277-1283
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Quantitative gas-liquid chromatographic methods for the determination of 5-propargyl2-furylmethyl dl-cis, trans-chrysanthemate, furamethrin, have been developed. The base line before the furamethrin peak of GLC drifted on the column with 2% silicone XE-60 coated on 60_??_80 mesh, acid-washed and DMCS-treated Chromosorb W (Method B), which suggested partial decomposition of furamethrin, however, a fine chromatogram was obtained when sodium borate was added to the column supporter (Method A). By utilizing an adequate internal standard, furamethrin was determined with the standard deviation of about 0.8% by Method B as well as Method A, the values being substantially equal to those obtained by the TLC-UV method previously reported. In addition, the trans and cis isomers in technical products of furamethrin were separated from each other on a 5% silicone DCQF-1 column and the results obtained by analyzing the mixtures indicate that the ratio can be accurately determined by their area ratio.
  • Masahiro OHSUGI, Han-Chul YANG, Koichi OGATA
    1972 年 36 巻 8 号 p. 1285-1291
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Microbial formation of biotin-vitamers from oleic acid was investigated. Many strains of bacteria which were able to utilize oleic acid as a sole carbon source were isolated from soils and other natural materials. Among these bacteria, some strains formed a biotinvitamer from oleic acid in the culture broth during the cultivation. The vitamer was purified from the culture broth of strain No. 23, and identified as desthiobiotin by chromatographical and biological methods.
    From the results of investigation on the taxonomical characteristics, the bacterial strain No. 23 was assumed to be Brevibacterium sp.
  • Masahiro OHSUGI, Mitsuko NAKAZAWA, Koichi OGATA
    1972 年 36 巻 8 号 p. 1293-1297
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Biotin-vitamer formation from salicylic acid was investigated. Strains of Pseudomonas sp., No. 102 and No. 362, isolated from soil samples utilized well salicylic acid as a sole source of carbon, and formed biotin-vitamers in culture broth. The metabolites were partially purified by the methods of active carbon adsorption and anion-exchange column chromatography, and clarified as desthiobiotin, bisnordesthiobiotin and 7-keto-8-aminopelargonic acid.
  • Toru KODAMA, Uichiro KOTERA, Koichi YAMADA
    1972 年 36 巻 8 号 p. 1299-1305
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    The purpose of the present investigation is to obtain the superior mutants from the tartrate producing strain, Gluconobacter suboxydans 2026Y2 previously isolated from nature. Some mutant strains obtained by treatment with N-methyl-N'-nitro-N-nitrosoguanidine were found to accumulate L(+) tartaric acid in culture broth with much higher yield than in the case of the wild strain.
    The high tartrate productivity of the mutants was followed by the low accumulation of 2-ketogluconic acid. The mutants having high assimilability of 5-ketogluconate showed high tartrate productivity.
    The culture conditions for tartaric acid production by a mutant, Gl. suboxydans N-3874, were investigated. As a result, the amount of tartaric acid accumulated in culture broth reached to _??_ level of 14.6g/liter in the medium containing 5% glucose and 0.3% corn steep liquor.
  • Uichiro KOTERA, Kazuyoshi UMEHARA, Toru KODAMA, Koichi YAMADA
    1972 年 36 巻 8 号 p. 1307-1313
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    The present purpose is to improve tartaric acid productivity of Gluconobacter suboxydans IAM 1829, which is well known as a 5-ketogluconic acid producer, by mutation involving the use of newly developed isolation method. In the course of studies for recognizing the causes suppressing the yield of tartaric acid, it was revealed that hydrogen-ion concentration and glycolic acid accumulated during fermentation limited the tartaric acid formation by inhibiting the growth of the bacteria. From these point of view, isolation of acid tolerant mutants and glycolate tolerant mutants was carried out. The significant correlation was found between the tartaric acid productivity of these mutants and their tolerance to those inhivitory agents, and some desireable mutants were obtained.
  • Uichiro KOTERA, Toru KODAMA, Yasuji MINODA, Koichi YAMADA
    1972 年 36 巻 8 号 p. 1315-1325
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    In the course of study on the mechanism of the tartaric acid formation from 5-ketogluconic acid, a new intermediary substance with mauve color to Abdel-Akhel and Smith's reagent was isolated from intact cell culture liquid. The chemical structure of this substance was determined as 1, 2-dihydroxvethyl hydrogen L(+) tartrate from the results of hydrolysis experiments and from the identifications of the constituents of the molecule, and named “pretaric acid.” Tartaric acid was evidently produced from pretaric acid by intact cell culture. Clearly, then, pretaric acid appears to be an intermediate in the formation of tartaric acid from 5-ketogluconic acid. The authors assumed that in the formation of pretaric acid from 5-ketogluconic acid, a Baeyer-Villiger type oxidation occurred.
  • Sawao MURAO, Susumu FUNAKOSHI, Kohei ODA
    1972 年 36 巻 8 号 p. 1327-1333
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    An acid protease of Cladosporium sp. No. 45-2 was purified and crystallized by precipitation with ammonium sulfate, fractional precipitation with acetone, and pH adjustment. About 600mg of third crystallized preparation was obtained from one liter of culture broth. The purified enzyme was chromatographically homogeneous and confirmed to be monodispersive by physicochemical criteria such as ultracentrifugal and electrophoretical analysis. The enzyme was most active at pH values between 2.5 and 2.7 toward both casein and hemoglobin and was stable at pH values from 2.5 to 7.0 on twenty hour incubation at 30°C.
    Millimolar concentration of sodium lauryl sulfate markedly inhibited the enzyme, vv heares diisopropyl phosphorofluoridate, sulfhydryl reagents, ethylenediaminetetra acetic acid, and divalent metal ion relatively little affected the activity. The enzyme was most resistant toward S-PI among the acid proteases tested.
  • Akira KAJI, Osamu YOSHIHARA
    1972 年 36 巻 8 号 p. 1335-1342
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    An α-D-galactosidase was purified from the culture filtrate of Corticium rolfsii IFO 6146 by a combination of QAE-Sephadex A-50 and SE-Sephadex C-50 chromatography. The purified enzyme was demonstrated to be free of other possibly interfering glycosidases and glycanases. The maximum activity of the enzyme towards p-nitrophenyl α-D-galactopyranoside was found to be at pH 2.5 to 4.5, and the enzyme was fairly active at pH 1.1 to 2.0. The enzyme was stable over a pH range 4.0 to 7.0 at 5°C for 72 hr and relatively unstable at pH 1.1 to 2.0 as compared with endo-polygalacturonase, α-L-arabinofuranosidase and β-D-galactosidase produced by C. rolfsii. The enzymic activity was completely inhibited by Hg2+and Ag+ ions, respectively. Km values were determined to be 0.16×10-3M for p-nitrophenyl α-D-galactopyranoside and 026×10-3M for o-nitrophenyl α-D-galactopyranoside. The values of Vmas were also determined to be 26.6μmoles and 28.6μmoles per min per mg for p- and o-nitrophenyl α-D-galactopyranoside, respectively.
  • Tadanobu NAKADAI, Seiichi NASUNO, Nobuyoshi IGUCHI
    1972 年 36 巻 8 号 p. 1343-1352
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    To elucidate the constitution of peptidases from Aspergillus oryzae, systematic separation of the enzymes was carried out by batchwise treatment with Amberlite IRC-50 and precipitation with rivanol. Proteases were separated to two fractions. They were Amberlite IRC-50 adsorbed and the non-adsorbed fractions and the latter fraction was further separated to two fractions, rivanol precipitable and non-precipitable fractions.
    Acid carboxypeptidase I was purified from the rivanol non-precipitable fraction by column chromatography on DEAE-cellulose, DEAE-Sephadex A-50 and SE-cellulose. The purified enzyme was not homogeneous on disc electrophoresis, although symmetric peaks were obtained for enzyme protein and activity in Sephadex gel filtration. The optimum pH is at pH 4.0 for earbobenzoxy-L-alanyl-L-glutamic acid. The enzyme activity was inhibited by SH reagents, but not inhibited by metal chelating agents. The molecular weight of the enzyme was estimated to be about 120, 000 by gel filtration.
  • Plastein from a Mixture of Soybean Protein Hydrolysate and L-Methionine Ethyl Ester
    Michiko YAMASHITA, Soichi ARAI, Keiichi Aso, Masao FUJIMAKI
    1972 年 36 巻 8 号 p. 1353-1360
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    With the aid of papain, a plastein was synthesized from a 1:10 mixture of Lmethionine ethyl ester and a peptic hydrolysate of soybean protein. Dialysis of the whole reaction-product yielded a methionine-incorporated plastein (Met-plastein) as the nondialyzable fraction, its yield being 78.2% on a dry-matter basis, of the whole reaction-product. Methionine content in this Met-plastein was 7.22% on a weight basis, while its content in the material hydrolysate was only 1.25%. Carboxypeptidase A treatment of Met-plastein liberated methionine at an outstandingly rapid rate. A similar, but not so outstanding, rate was observed for the methionine liberation from Met-plastein by treatment with leucine aminopeptidase. Methyl isothiocyanate treatment and subsequent cyclization yielded a mixture of methylthiohydantoins from Met-plastein. Gas chromatographic analysis of this mixture after trimethylsilylation showed a result that methionine occupied 33.2%, on a molar basis, of the total N-terminal amino acids. Lithium borohydride reduction and 6 N hydrochloric acid hydrolysis of Met-plastein produced monomeric aminols, and their 2, 4-dinitrophenylation followed by thin-layer chromatography gave a result that methionine occupied 84.9%, on a molar basis, of the total C-terminal amino acids; the residues amounting to 14.4%, of the C-terminal methionine residues remained as an ethyl eater form. The selective degradation probe employing cyanogen bromide to generate free homoserine disclosed that the occurrence of the polymeric methionine-methionine sequence was little if any in Met-plastein. Based on the above experiments as well as an evaluation of the esterase activity against L-methionine ethyl ester, a possible mechanism was discussed of the papain-catalyzed synthesis of plastein in a system containing such an ester.
  • Kenichi HISATSUKA, Tadaatsu NAKAHARA, Koichi YAMADA
    1972 年 36 巻 8 号 p. 1361-1369
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    During the study of the n-alkane oxidation by Pseudomonas aeruginosa S7B1, a nondialysable activator for n-alkane oxidation was discovered in the culture broth of the strain. The activator was purified, as judged by cellulose acetate membrane electrophoresis, by ammonium sulfate precipitation and chromatography on DEAE-Sephadex, CD4-Sephadex and Sephadex G-75 columns.
    The purified activator, which was positive in protein color reactions, remarkable stimulated only the oxidation of n-hexadecane, though it was not observed in case of palmitic acid or glucose oxidation.
    Co-operative action between the activator and rhamnolipid, which had been isolated as a growth stimulant of P. aeruginosa S7B1 on n-hexadecane by the authors, was observed not only in the oxidation of n-hexadecane but also in the growth on n-hexadecane.
  • Yoshinori OZAWA, Kazuo SUZUKI, Osamu TAJIMA, Koya MOGI
    1972 年 36 巻 8 号 p. 1371-1380
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Pattern analysis of peptides in soy sauce was tried by gel filtration and ion exchange chromatography. In gel filtration through Sephadex G-15, the separation of smaller peptides and amino acids in soy sauce was found to be not so critical. Subsequently the mapping of peptides in soy sauce was done by ion exchange chromatography with Dowex 50-X2. Consequently it was estimated that there existed more than ten kinds of peptides in soy sauce.
    Moreover, in the mapping of peptides, the automated measurement by the use of a discrete type analyzer, EEL Auto Chemist, was tried. The method developed here was not fully automated but available and effective for the mapping of peptides eluted from an ion exchange resin column.
  • Noboru SUGIYAMA, Hideo SHIMAHARA, Tetsuo ANDOH, Motoko TAKEMOTO, Toshi ...
    1972 年 36 巻 8 号 p. 1381-1387
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    By light scattering method, the molecular weights and the radii of gyration of konjac mannans from various sources have been studied. The results are: (1) The molecule of konjac mannan suffered degradation into almost one-fourth by acetylation or by the traditional preparation of konjac flour. (2) The differences in rheological properties of the rigid gels from different strains could be interpreted in terms of the light scattering measurements. (3) The peculiar properties of the Shina-shu have been explained by its Zimm plot behavior at the part of low angle. (4) The distinct differences have been observed in the values of Mw and RG of the same strains porduced from different areas. Good pararellism has been found between the traditional “Mizuhiki-test” and the results of light scattering measurements.
  • Ryosuke UCHIO, Isamu SHIIO
    1972 年 36 巻 8 号 p. 1389-1397
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    The better condition of cultivation for tetradecane 1, 14-dicarboxylic acid (DC-16) production from n-hexadecane (n-C16) by Candida cloacae MR-12 was investigated by using acetic acid as carbon source for the growth. In general, the condition suitable for the growth was also favorable for the production of DC-16. The change of pH during cultivation, the use of NaOH solution as pH controlling agent after pH-change and the addition of antifoam stimulated the production of DC-16.
    Under the optimum conditions where the culture medium contained 15%(v/v) n-C16, 1.4%(w/v) acetic acid, inorganic salts and growth factors, and pH was changed from 6.5 to 7.75 at 16 hr after the inoculation, the highest level of DC-16 production was attained after about 72 hr cultivation and the amount of the product accumulated was 61.5g per liter of the medium.
    When a mixture of various n-alkanes was used as starting material, DCs corresponding to the respective n-alkanes were produced as mixture.
  • Hajime OHIGASHI, Kazuyoshi KAWAZU, Hiroshi EGAWA, Tetsuo MITSUI
    1972 年 36 巻 8 号 p. 1399-1403
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    An antifungal constituent, C9H14O3, containing allenic double bond, was isolated from the leaves of Sapium japonicum (Euphorbiaceae). The structure of this antifungal constituent was shown to be methyl 8-hydroxv-5, 6-octadienoatc.
  • Haruo TANAKA, Kiyoshi NAKAYAMA
    1972 年 36 巻 8 号 p. 1405-1412
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Brevibacterium ammoniagenes ATCC 6872 was previously reported to accumulate large amounts of IMP, AMP, ADP, ATP, GMP, GDP and GTP from the corresponding purine bases. The organism was also reported to convert various derivatives of purine and 8-azapurine to the corresponding ribotides.
    Using the similar process, ribotidation of pyrazolo [3, 4-d] pyrimidines was attempted, and it was found that the same organism was able to produce remarkable amounts of 4-hydroxy-1-β-D-ribofuranosylpyrazolo [3, 4-d] pyrimidine 5'-monophosphate (HPP-RP) from 4-hydroxy-pyrazolo [3, 4-d] pyrimidine (HPP, allopurinol) and 4-amino-l-β-D-ribofuranosylpyrazolo [3, 4-d] pyrimidine 5'-monophosphate and 5'-diphosphate from 4-amino-pyrazolo [3, 4-d] pyrimidine.
    The crystals of HPP-RP (Na-salt) were isolated from the cultured broth of Br. ammoniagenes incubated with HPP, and characterized based on UV-spectra, IR-spectrum, NMR and others.
    It was also found that HPP-RP was converted to the corresponding riboside by hydrolysis in aqueous solution (pH 4.0_??_9.0) for 6 hr at 140°C. The hydrolysis of HPP-RP was also accomplished with various organisms.
  • Seiya OGATA, Osamu MIHARA, Yoshifumi IKEDA, Motoyoshi HONGO
    1972 年 36 巻 8 号 p. 1413-1421
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Two new kinds of high molecular bacteriocin, named as clostocins 0 and M, were found in Clostridium saccharoperbutylacetonicum and its related strains. Production of both clostocins was inducible by mitomycin C (MC) or ultraviolet ray. The active clostocins appeared in the bacterial cells at about 105 min after MC-treatment, increased rapidly during next 75 min and then released into the medium with the cell lysis. The clear lysis of 0- and M-producing cells was observed at 3.5 hr and 4 hr respectively after MC-treatment. Clostocin O killed M-producing strains, and clostocin M did 0-producing strains. Electron microscopic observation revealed that clostocins 0 and M were phage tail-like particles with contractile sheath round a core. The particles resembled some pyocins and also the tail of phage HM 3 of Cl. saccha rope rbu tylace ton icum.
    It was also found that M-producing strains had simultaneously the ability of production of low molecular bacteriocin, named as clostocin D.
  • Teruyoshi MATOBA, Tadao HATA
    1972 年 36 巻 8 号 p. 1423-1431
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Various peptides and derivatives of peptides and amino acids were synthesized and tasted, systematically, to elucidate the relationship between bitterness and chemical structures of peptides.
    We have found that: 1. Peptides become more bitter than the original amino acids when their amino and carboxyl groups are blocked and when peptide bond is formed. 2. A peptide molecule with a high content of amino acids with hydrophobic side chains will develop bitter taste. 3. The amino acids in a peptide chain independently contribute to bitterness regardless of amino acid sequences and configuration.
  • Eitaro MASUO, Toshio NAKAGAWA
    1972 年 36 巻 8 号 p. 1433-1436
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
    Based on three measures of evaluation, it was concluded that with respect to pseudomonads the learning machine was superior to experienced microbiologists in predicting the presence or absence of the following four features; the production of phenazine compounds, the utilizations of D-sorbitol, of n-hexadecane, and of histamine.
  • Minoru SAITO, Yoshitsugu HARADA, Kazunori MIZOGUCHI, Munehiro HIRAYAMA
    1972 年 36 巻 8 号 p. 1437-1439
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
  • Tomoya OGAWA, Motoshi YASUI, Masanao MATSUI
    1972 年 36 巻 8 号 p. 1441-1442
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
  • Tomoya OGAWA, Motoshi YASUI, Masanao MATSUI
    1972 年 36 巻 8 号 p. 1443-1444
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
  • Tomoya OGAWA, Motoshi YASUI, Masanao MATSUI
    1972 年 36 巻 8 号 p. 1445-1447
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
  • Tomoya OGAWA, Masanao MATSUI, Hiroshi OHRUI, Hiroyoshi KUZUHARA, Sakae ...
    1972 年 36 巻 8 号 p. 1449-1451
    発行日: 1972年
    公開日: 2008/11/27
    ジャーナル フリー
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