-
Tsutomu SUGIURA, Yasuhide OTA, Yasuji MINODA
1975 年 39 巻 9 号 p.
1689-1694
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Unsaturated long-chain fatty acids such as oleic, linoleic and ricinoleic acid except for elaidic acid were effective on the lipase production by
Candida paralipolytica, but saturated fatty acids were not effective. When elaidic and myristic acids were dissolved in
n-hexadecane to be dispersed in a liquid state, they became to be effective on the lipase production. These results suggest that long-chain fatty acids of a liquid state are effective on the lipase production. Lipase activator A and phosphatidylethanolamine stimulated the effect of fatty acids on the lipase production. Effects of sterols and surfaceactive substances on the lipase production were also reported.
A weak tributyrin-hydrolyzing activity, in the absence of fatty acids, was found in the yeast cell.
抄録全体を表示
-
Kenji SAKAGUCHI, Ryuichiro KURANE, Machiko MURATA
1975 年 39 巻 9 号 p.
1695-1702
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Two fungi were isolated from soil which grew on 0.1_??_0.2% formaldehyde as the sole carbon source, and identified as
Gliocladium deliquescens and
Paecilomyces varioti. Both the strains could grow on 5% methanol and 5% Na-formate, while the former could grow even on 7% methanol. Metabolic pathways were traced through two dimensional paper chromatography and autoradiographic techniques using
14C-formaldehyde,
14C-methanol or
14C-CO
2 as substrates.
The intracellular metabolites were persued and their quantitative variation with time was measured. Along with the fact that serine and malate appeared in the earlier time, then appeared organic acids and amino acids belonging to TCA cycle, and the fact that hydroxy-pyruvate reductase and phosphoenolpyruvate carboxylase activities were much stronger in methanol culture than in ethanol culture, it was concluded that the two fungi followed the serine pathway in assimilating C
1-compounds. Oxidation enzymes of methanol and formaldehyde were also studied, and an oxidizing system was found besides usual NAD linked methanol or formaldehyde dehydrogenases.
抄録全体を表示
-
Kazuhiko FUKAGAWA, Haruki YAMAGUCHI, Osamu UOTANI, Toshio TSUJIMOTO, D ...
1975 年 39 巻 9 号 p.
1703-1710
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The presence of O-acetyl ester on fucogalactan was suggested by IR spectrum and confirmed by gas-liquid chromatographic analysis. The determination of acetyl ester revealed the complete molar composition of fucogalactan to be fucose:galactose:acetic acid=1:1:0.6. Sequential Smith degradation of the deacetylated fucogalactan gave a polysaccharide resistant to periodate oxidation, except for the terminal residues, suggesting the presence of a 1→3-linked core galactose-chain. Gas-liquid chromatographic analyses of hydroxy- and oxo-compounds produced by Smith degradation showed that all fucose residues were located in the side chains with some of galactoses. Further, every side chain was shown to be mainly composed of 1→2-and/or 1→6-linked sugars and terminated with fucose residue. These structural features were consistent with the results of linkage analyses of the partial acetolysis products from fucogalactan. The deacetylation of fucogalactan reduced greatly the intrinsic viscosity and increased the sedimentation coefficient.
抄録全体を表示
-
Kazuho WATANABE, Takashi FUKIMBARA
1975 年 39 巻 9 号 p.
1711-1717
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The structure of the carbohydrate moiety of GP-I-b which is one out of three glycopeptides isolated from a Pronase digest of the saccharogenic amylase of
Rhizopus javanicus sp. 3-46, was investigated by enzymatic and chemical techniques.
Nine moles of mannose followed by one mole of
N-acetylglucosamine were released per mole of GP-I-b when it was treated sequentially with purified jack bean α-mannosidase and β-
N-acetylglucosaminidase.
Methylation of GP-I-b gave 3, 6-di-
O-methyl derivative from the
N-acetylglucosamine residues, and 2, 3, 4, 6-tetra-
O-methyl, 3, 4, 6-tri-
O-methyl and 2, 4-di-
O-methyl derivatives from the mannose residues in an approximate ratio of 3:4:2.
A smaller glycopeptide (F-1) containing two moles each of mannose and
N-acetylgluco-samine per mole of asparagine was obtained when GP-I-b was subjected to one step of the Smith degradation. Exhaustive methylation of F-1 gave 3, 6-di-
O-methyl derivative of
N-acetylglucosamine, and 2, 3, 4, 6-tetra-
O-methyl and 2, 3, 4-tri-
O-methyl derivatives of mannose in a ratio of 1.00:0.85.
Controlled acetolysis of GP-I-b yielded mannose,
O-α-mannosyl-(1→2)-
O-α-mannosyl-(1→3)-mannose and a smaller glycopeptide which was resistant to the acetolysis.
From these and previous evidences, the following structure was determined for GP-I-b.
抄録全体を表示
-
Isao UEYAMA, Iwao TAKASE
1975 年 39 巻 9 号 p.
1719-1727
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The determination of residues in fresh milk and the metabolic fate of edifenphos (
O-ethyl
S, S-diphenyl phosphorodithiolate), the active ingredient of the fungicide HINOSAN
® were conducted in female goats using gas-liquid chromatographic techniques. Edifenphos was rapidly metabolized to form polar products which were excreted in the urine. The major metabolites found in the urine were
O-ethyl
S-phenyl hydrogen phosphorothiolate and
O-conjugates of
m- and
p-(hydroxyphenyl) methyl sulfone. Several other metabolites;
S, S-diphenyl hydrogen phosphorodithiolate,
S-phenyl dihydrogen phosphorothiolate,
O-ethyl dihydrogen phosphate,
O-ethyl
S-phenyl hydrogen phosphorodithiolate and methyl phenyl sulfone were also identified. In the feces, blood, some organs and tissues, edifenphos was scarcely detected. Oral administration of edifenphos to goat at the rate of 10mg/kg resulted the secretion of 0.0008 ppm of edifenphos in milk as the maximum level after 6 hr of the administration.
抄録全体を表示
-
Takeshi SASSA, Akihiro TAKAHAMA, Tamotsu SHINDO
1975 年 39 巻 9 号 p.
1729-1734
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The stereostructure of cotylenol has been assigned as I on the basis of degradative and spectroscopic evidence. Cotylenol is a novel diterpene with ophiobolane skeleton.
抄録全体を表示
-
Takeshi SASSA, Mitsuo TOGASHI, Tomio KITAGUCHI
1975 年 39 巻 9 号 p.
1735-1744
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The structures of cotylenins, leaf growth substances produced by a fungus, have been assigned on the basis of degradative and spectroscopic evidence. They are novel glycosides with a common aglycone, cotylenol: cotylenins A and C have unusual sugar moieties consisting of an 6-O-methyl-α-D-glucosyl derivative with an oxygenated C
5-isoprene unit.
抄録全体を表示
-
Mikio SHIMIZU, Taketoshi MASUIKE, Hironori FUJITA, Takao IIDA, Kazuo K ...
1975 年 39 巻 9 号 p.
1745-1748
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Kluyvera citrophila KY7844 enzymatically catalyzed N-acylation of 7-amino-desacetoxycephalosporanic acid with 1-(1H)-tetrazolylacetate methylester to produce 7-[1-(1H)-tetrazolylacetamido]-desacetoxycephalosporanic acid. The product was purified and characterized.
抄録全体を表示
-
Sadanobu TOBE, Toru TAKAMI, Yoshiteru HIROSE, Koji MITSUGI
1975 年 39 巻 9 号 p.
1749-1755
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
An alkaline proteinase produced by
Bacillus sp. was purified and crystallyzed through isopropanol or ammonium sulfate precipitation, decolorization with DEAF-cellulose and gel filtration with Sephadex G-100. The optimum pH for caseinhydrolysis was 11.5 and the activity was completely inactivated after incubation with DFP. The specific activity on Hammersten casein was about 4, 500 units/mg enzyme protein. The enzyme also hydrolyzed synthetic ester substrate such as Ac-Tyr-OEt, Ac-Phe-OEt or Bz-Met-OMe. The isoelectric point was pH 10.7, and the molecular weight was estimated to be about 17, 500 by the sedimentation equilibrium method and 16, 000 by gel filtration method. Some physicochemical properties and the amino acid composition of the enzyme were investigated, indicating that the enzyme is distinguishable from alkaline proteinase of
Bacillus species so far reported.
抄録全体を表示
-
Shintaro KAMIYA, Sachiko ESAKI, Fukuko KONISHI
1975 年 39 巻 9 号 p.
1757-1762
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Naringenin-7-β-maltoside (I), -7-β-cellobioside (II), -7-β-lactoside (III), -7-β-melibioside (IV) and hesperetin-7-β-[D-galactosyl (α 1→2) D-glucoside] (V), -7-β-[D-glucosyl (β 1→2) D-galactoside] (VI) and -7-β-melibioside (VII) were prepared by the coupling of naringenin or hesperetin with the acetobromo derivatives of appropriate disaccharides followed by removal of the protecting acetyl groups.
Narigenindihydrochalcone-4'-β-kojibioside (VIII), -4'-β-maltoside (IX), -4'-β-cellobioside (X), -4'-β-lactoside (XI), -4'-β-melibioside (XII) and hesperetindihydrochalcone-4'-β-[D-galactosyl (α 1→2) D-glucoside] (XIII), -4'-β-sophoroside (XIV) and -4'-β-melibioside (XV) were synthesized by catalytic reduction of the appropriate flavanone-7-β-glycosides.
Among the compounds synthesized, IX and X are 4 and 8 times as sweet as sucrose on the basis of percentage concentration, respectively, but the others are tasteless.
抄録全体を表示
-
Masayuki NAKAGAWA, Mitsuru ANDO, Yoshiko OBATA
1975 年 39 巻 9 号 p.
1763-1773
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The persistence and degradation of isoxathion
14C-labeled at the 5-position of the isoxazole ring were studied in three soil types under laboratory conditions. Persistence was influenced by soil type and moisture content; approx. half life at 30 ppmw dose level varied from 15 to 40 days in nonflooded models. In a flooded model isoxathion disappeared much faster. Isoxathion underwent biodegradation to a number of products with concomitant release of
14CO
2. 3-Hydroxy-5-phenylisoxazole, 5-phenyl-4-oxazolin-2-one, benzoylacetamide and benzoic acid were the unequivocally identified metabolites; oxon derivative of isoxathion, 3-methoxy-5-phenylisoxazole, 2-methyl-5-phenyl-4-isoxazolin-3-one, 2-acetyl-5-phenyl-4-isoxazolin-3-one, 2, 5-diphenylpyrazine and acetophenone were tentatively identified as the minor products. None of these major products was persistent in soils. 3-Hydroxy-5-phenylisoxazole, the initial metabolite or hydrolyzate of isoxathion, was adsorbed to soil to a much greater extent than isoxathion, which explains the rapid disappearance of its fungicidal activity in soil.
抄録全体を表示
-
Toshio OMORI, Yoshifumi JIGAMI, Yasuji MINODA
1975 年 39 巻 9 号 p.
1775-1779
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
As a result of screening isoalkyl or isoalkenyl substituted aromatic hydrocarbon assimilating microorganisms, 19 strains of isopropylbenzene assimilating bacteria were isolated. Thirteen of these strains were found to grow on ct-methylstyrene and all 4 strains tested were also found to grow on isobutylbenzene.
Among them, 2 strains (S 107 Bl and S 182 Bl) were selected for further study and were identified as
Ps. convexa and
Ps. ovalis, respectively.
Furthermore, some examined aromatic hydrocarbon utilizing bacteria were classified into two groups by differences in substrate assimilation specificity.
抄録全体を表示
-
Yoshifumi JIGAMI, Toshio OMORI, Yasuji MINODA
1975 年 39 巻 9 号 p.
1781-1788
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
To clarify biodegradation pathways of isoalkyl substituted aromatic hydrocarbons, oxidation products of isopropylbenzene and isobutylbenzene by
Ps. desmolytica S449B1 and
Ps. convexa S107B1 were examined.
Oxidation products from isopropyl benzene were determined to be 3-isopropylcatechol and (+)-2-hydroxy-7-methyl-6-oxooctanoic acid. Isobutylbenzene was also oxidized to 3-isobutylcatechol and (+)-2-hydroxy-8-methyl-6-oxononanoic acid by the same strains.
From these results, the existence of an unknown reductive step in the degradation of these isoalkyl substituted aromatic hydrocarbons and the initial oxidation of these aromatic hydrocarbons by the strains were made clear. The degradation pathways of isopropyl benzene and isobutylbenzene by these strains were discussed.
抄録全体を表示
-
Minoru YOSHIMURA, Shigeru YAMANAKA, Koji MITSUGI, Yoshio HIROSE
1975 年 39 巻 9 号 p.
1789-1795
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The production of pigment by the molds belonging to the genus
Monascus in a submerged culture was examined. The extracellular pigment was mainly studied.
Monascus sp. No. 2 was found to be the most potent pigment producer. The optimum cultural conditions were: pH of the medium, 6.5; the temperature, 25°C; carbon sources, glucose or ethyl alcohol; nitrogen sources, polypeptone, yeast extract, monosodium glutamate or casamino acids. Glycine, L-threonine, L-arginine, L-alanine and L-tyrosine were found to be the most effective substances promoting pigment production.
Mycelial forms of this strain were correlated with pigment formation in submerged culture. As it grew into pellet type, the yield of pigment was at high level.
The
Monascus-pigment in the fermentation liquid seemed to be firmly bound to the proteinlike substances which made the pigment apparently soluble.
抄録全体を表示
-
Toshihiro NAKANISHI, Takehiko YAMAMOTO
1975 年 39 巻 9 号 p.
1797-1802
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The sites on oxidized insulin B-chain substrate initially attacked by an alkalophilic proteinase from a
Streptomyces sp., were investigated under incubation conditions employing one part enzyme to one thousand parts of substrate at 0°C.
Analysis of the peptides produced after 10 to 40 seconds of incubation revealed that the enzyme, which has an optimum pH of around 13, first attacks two peptide linkages “-Leu (15)-_??_Tyr (16)-_??_Leu (17)-” of the oxidized insulin B-chain with equal efficiency.
抄録全体を表示
-
Tsuyoshi NAKAMATSU, Teiko AKAMATSU, Ryuichi MIYAJIMA, Isamu SHIIO
1975 年 39 巻 9 号 p.
1803-1811
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Productivity of extracellular glucose oxidase was examined for various microorganisms and it was found in strains belonging to genus
Penicillium except one species of
Tallalomyces.
As the best glucose oxidase producer,
Penicillium purpurogenum No. 778 was isolated from natural source. This microorganism produced 32, 000 units per ml broth of glucose oxidase in a simple medium containing beet molasses, NaNO
3 and KH
2PO
4 by submerged culture for 3 days. That value was about 10-times of that of
Penicillium amagasakiense which has been known as an excellent glucose oxidase producer.
Culture conditions for glucose oxidase production were examined, which were extremely different among microbial species. In the case of
Penicillium chrysogenum AJ 7007 and
Penicillium purpurogemun No. 778, the effects of aeration and carbon sources were remarkably different from each other.
Penicillium purpurogenum No. 778 produces catalase sufficiently in a culture broth for glucose oxidase application in food industry.
Glucose oxidase was purified about 25-fold from culture supernatants of
Penicillium purpurogenum No. 778, and some properties of the enzyme were examined. The optimum temperature and pH for the activity were 35°C and 5.0, respectively. The enzyme was stable at pH 5.0 to 7.0 when it was incubated at 40°C for 2 hr, while it was stable at temperature lower than 50°C when incubated at pH 5.6 for 15min. The enzyme was specific for D-glucose and apparent Michaelis constant for D-glucse was 12.5mM. The enzyme was inhibited by 1mM of HgCl
2, CuSO
4, NaHSO
4 and phenylhydrazine, but not inhibited by 1mM of
p-hydroxy-mercuribenzoate, EDTA, hydroxylamine and dimedone. Four percents NaCl inhibited the activity about 50%, while the addition of ethanol (from 0 to 16%) increased oxygen uptake more than that expected from the peroxidase activity of catalase.
抄録全体を表示
-
Mamoru ARAI, Masaki NAKAHARA, Kiyoshi HAMANO, Hiroshi OKAZAKI
1975 年 39 巻 9 号 p.
1813-1819
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Systematic isolation of the cell constituents of
Proteus mirabilis RMS-203 was performed to find out localization of antitumor principle only in the lipopolysaccharide (LPS) layer of the cell wall fraction.
LPS with strong antitumor activity was extracted from
P. mirabilis RMS-203 by phenol-water method followed by purification on DEAE-Sephadex A-50 column chromatography.
The main components of purified LPS were galactose, hexosamine, 2-keto-deoxy-octonic acid (KDO), myristic acid, β-hydroxymyristic acid and α, ε-diaminopimelic acid.
The minimal effective dose of LPS against Ehrlich solid carcinoma in mice was 0.1_??_1.0 μg/mouse. LD
50 in mice and pyrogenicity in rabbits were 28mg/kg and 10
-3-10
-5 μg/rabbit, respectively.
抄録全体を表示
-
Masaki NAKAHARA, Nobuaki KITAHARA, Kiyoshi HAMANO, Mamoru ARAI, Hirosh ...
1975 年 39 巻 9 号 p.
1821-1826
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Studies on lipopolysaccharide (LPS) from the cells of
Proteus mirabilis RMS-203 were focused upon reduction of lethal toxicity and of pyrogenicity by biological and chemical modification. A heptoseless mutant, strain N-434, was isolated by the use of phage resistancy as a tool. LPS from that heptoseless mutant was completely deficient in neutral sugars and mainly composed of 2-keto-deoxy-octonic acid (KDO), glucosamine and fatty acids. It revealed almost the same antitumor activity as LPS of the wild type but it was less toxic and less pyrogenic.
Hydroxylaminolysis and reduction with LiAlH
4 resulted in removal of fatty acids from LPS accompanied with decrease in lethal toxicity and antitumor acitivity but not in pyrogenicity.
Lipid A fractions showed almost the same antitumor activity as intact LPS but less lethality and less pyrogenicity.
抄録全体を表示
-
Toyokazu NISHINO, Sawao MURAO
1975 年 39 巻 9 号 p.
1827-1833
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The molecular weight determined by the sedimentation equilibrium and SDS polyacrylamide gel electrophoresis was 29, 000 and 28, 000, respectively. Isoelectric point of the enzyme was determined as pH 7.7. This enzyme contained large amounts of alanine, aspartic acid, glutamic acid and serine, and no cysteine residue was found. The enzyme was inhibited by SDS, KMnO
4, EDTA and tetracycline. GTP and GDP were the most active as pyrophosphate acceptor to the enzyme. The apparent
Km for ATP was 2.2×10
-4M and that for GTP was 2.1×10
-4M in the reaction of ATP+GTP→AMP+pppGpp. On the other hand, in the reaction of 2ATP→AMP+pppApp, the apparent
Km for donor and acceptor ATP was 1.7×10
-3M. Effects of pH and metal ions on the enzymatic synthesis of pppGpp were also studied.
抄録全体を表示
-
Choemon KANNO, Makoto SHIMIZU, Kunio YAMAUCHI
1975 年 39 巻 9 号 p.
1835-1842
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
A soluble apoprotein fraction was prepared from milk fat globule membrane lipoproteins by delipidation with a chloroform-methanol mixture and was fractionated into three fractions by gel filtration on Bio-Gel A-5m.
The major fraction, Fraction II, contained about 30% of carbohydrate,
i.e. 13.9% of hexoses, 8.1% of hexosamines, 8.0% of sialic acid and 0.8% of fucose, and was therefore designated a soluble glycoprotein fraction. The fraction was apparently homogeneous on sedimentation velocity analysis and DEAE-Sephadex chromatography, and had
s020, W 6.1,
D020, W 3.79, _??_0.719,
f/f0 2.16 and molecular weight 139, 000 daltons. However, the diffused pattern on disc electrophoresis and the occurrence of plural N-terminal amino acid residues suggest that the protein of this fraction is likely to be formed by intermolecular association of heterogeneous polypeptide chains.
抄録全体を表示
-
Masaaki YOSHIKAWA, Etsuro SUGIMOTO, Hideo CHIBA
1975 年 39 巻 9 号 p.
1843-1849
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
The interaction of α
S1-casein with β-, dephosphorylated β-, γ- and R-caseins was studied. It was proved by the sedimentation velocity experiments that α
Sl-casein formed a complex with each of these components at 25°C in the presence of 3mM CaCl
2.
In the presence of 10mM CaCl
2, β- and dephosphorylated β-casein prevented the precipitation of α
S1-casein and gave micelle-like turbid solutions. However, γ- and R-caseins, fragments of β-casein, did not stabilize α
S1-casein. It was concluded from these results that β-casein interacted with α
S1-casein through its hydropholic region corresponding to R-casein and that hydrophilic region of β-casein was responsible for the stabilization of α
S1-casein.
抄録全体を表示
-
Akio KOBAYASHI, Hiroshi EGAWA, Koichi KOSHIMIZU, Tetsuo MITSUI
1975 年 39 巻 9 号 p.
1851-1856
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Pterosins B and O (pterosin B methyl ether) were isolated as antimicrobial constituents from
Pteris inaequalis Bak.
aequata Tagawa.
抄録全体を表示
-
Ikuzo URITANI, Tetsuo SAITO, Hachiro HONDA, Won K. KIM
1975 年 39 巻 9 号 p.
1857-1862
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
When sweet potato root tissues were infested by the larvae of sweet potato weevil,
Cylas formicarius and West Indian sweet potato weevil,
Euscepes postfasciatus, furano-terpenoids and coumarins were produced in brown necrotic layer formed during the infestation.
The larval homogenates of both weevils also induced in the tissue the production of furanoterpenoids and coumarins, as well as the formation of necrotic layer. The larval homogenate of sweet potato weevil induced also ethylene formation, the marker of injury in the tissue. Investigations on the furano-terponoid inducing factor demonstrated that the factor was 20mM KCl-soluble, non-dialyzable, acetone-precipitable, (NH
4)
2SO
4-precipitable, heat-unstable, passing through Sephadex G-25 column without sieving and partially inactivated by pronase, indicating that the factor was a high molecular weight compound, perhaps of a proteinacious property. It is likely that the factor causes injury or death to sweet potato root tissue, leading to the formation of ethylene and necrotic layer, and then to production of furano-terpenoids and coumarins.
抄録全体を表示
-
Shigeru YAMAMOTO, Toshiaki SHINOHARA
1975 年 39 巻 9 号 p.
1863-1868
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
Blood group H-like polysaccharides were isolated from seeds of
Euonymus Sieboldiana by a procedure that included fractionation with (NH
4)
2SO
4, heat treatment, chromatography on DEAE-cellulose and gel filtration on Bio-Gel P-150. One of the highly purified polysaccharide fractions was composed of arabinose, mannose, glucose, rhamnose, galactose and fucose. Arabinose and mannose were the main components, and their molar ratio was calculated as about 3:1 by gas chromatographic analysis. An analytical ultracentrifugal experiment revealed that the finally purified H-like substance was close to an homogeneous preparation with a small disperse fraction. This heteropolysaccharide inhibited the haemagglutination of human group O red blood cells and eel anti-H serum minimally at 0.004mg/ml, reacted also with the eel anti-H serum on an immunodiffusion plate to form sharp precipitin line (s).
抄録全体を表示
-
Kazuo MATSUMOTO, Yuji URABE, Yasuhiko OZAKI, Tameo IWASAKI, Muneji MIY ...
1975 年 39 巻 9 号 p.
1869-1873
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
threo-4, 4, 4-Trichlorothreonine was synthesized completely stereoselectively through
trans-4-alkoxycarbonyl-5-trichloromethyl-2-oxazoline, which was prepared almost quantitatively by the reaction of 2-isocyano acetate with chloral in the presence of an organic base. Several derivatives of these compounds were also synthesized.
Furthermore, the stereochemistry of the resulting trichlorocompounds was chemically elucidated. The trichlorothreonine was easily converted into
threo-threonine by hydrogenation over Pd/C in the presence of NaHCO
3.
抄録全体を表示
-
P. ZAFFARONI, N. ODDO, R. OLIVIERI, L. FORMICONI
1975 年 39 巻 9 号 p.
1875-1877
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Yôtaro KONDO
1975 年 39 巻 9 号 p.
1879-1881
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Jun-ichi AZUMA, Naoki KASHIMURA, Tohru KOMANO
1975 年 39 巻 9 号 p.
1883-1884
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Tadayoshi MORIYA, Akira TANAKA, Kyohei YAMASHITA
1975 年 39 巻 9 号 p.
1885-1886
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Yoshiaki NAKAHARA, Masanao MATSUI
1975 年 39 巻 9 号 p.
1887-1888
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Kenji MORI, Shin-ichi KOBAYASHI, Masanao MATSUI
1975 年 39 巻 9 号 p.
1889-1890
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Takaaki FUJII, Kenzo TONOMURA
1975 年 39 巻 9 号 p.
1891-1892
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Sawao MURAO, Yasutaro HAMAGISHI, Toyokazu NISHINO
1975 年 39 巻 9 号 p.
1893-1895
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Shunro KAWAKISHI, Yukio KITO, Mitsuo NAMIKI
1975 年 39 巻 9 号 p.
1897-1898
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Takeshi SASSA, Koichi NAKANO, Yukichi MIURA
1975 年 39 巻 9 号 p.
1899-1900
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー
-
Hiromichi NAGASAWA, Akira ISOGAI, Katsutoshi IKEDA, Shouhachi SATO, Sh ...
1975 年 39 巻 9 号 p.
1901-1902
発行日: 1975年
公開日: 2008/11/27
ジャーナル
フリー