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Eiji GOTO, Tohru KODAMA, Yasuji MINODA
1978 Volume 42 Issue 7 Pages
1305-1308
Published: 1978
Released on J-STAGE: November 27, 2008
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Growth characteristics of thermophilic hydrogen bacteria TH-1, and TH-4 were examined and taxonomic studies of these organisms were performed. Both Strains assimilated various organic acids but not the tested sugars. Autotrophic growth utilizing hydrogen, oxygen and carbon dioxide was more rapid than heterotrophic growth on organic acid media. With pyruvate as the sole carbon source, TH-1 grew more rapidly under oxy-hydrogen atmosphere (H
2:0
2=4:1) than in air. Neither TH-1 nor TH-4 fit the description of any previously de-scribed species; and they are thus proposed as new species,
Pseudomonas hydrogenothermophila nov. sp. Goto, Kodama and Minoda and
Flavobacterium autothermophilum nov. sp. Goto, Kodama and Minoda, respectively.
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Akira HIRAMATSU, Takeshi OUCHI
1978 Volume 42 Issue 7 Pages
1309-1313
Published: 1978
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Streptomyces naraensis was inoculated into 100ml of culture broth, containing 50 mu;Ci of
65Zn, diluted with ZnCl
2 solution to make 10
-4M Zn
2+ ion, at 27°C for 5 days with shaking.
65Zn-labeled neutral proteinase from
Streptomyces naraensis was prepared by the method described previously.
3) The preparation was homogeneous by disc electrophoresis and contained 1 g-atom of zinc per mole of enzyme in calculation by radioactivity.
It was suggested that the protein-bound zinc of neutral proteinase was not essential for enzymatic activity. Thus, this zinc was an essential component for the higher order structure of the protein, and the removal of zinc treated with EDTA
* inactivated the enzyme. The enzy-matic activity was maintained in the nresence of calcium ion.
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Yoshikatsu SUZUKI, Akira KAWARADA
1978 Volume 42 Issue 7 Pages
1315-1321
Published: 1978
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Six new products of oxidation of indolyl-3-acetic acid catalyzed by horseradish peroxidase were isolated, along with four known ones, 3-hydroxymethyloxindole (1), 3-methyleneoxindole (2), indolyl-3-aldehyde (4), and 3, 3-diindolylmethane (10). Based on spectroscopic and chemi-cal evidence, the new products were identified as 3-acetoxyindole (3), 3-(indol-3-ylmethyl)-oxindole (6), 3-[(2-indol-3-ylmethyl)indol-3-ylmethyl]oxindole (9), the 3-hydroxymethyl com-pounds of 6 and 9 (5 and 7), and 2-(indol-3-ylmethyl)indolyl-3-acetic acid (8), respectively.
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Hiromi YOSHIDA, Goro KAJIMOTO
1978 Volume 42 Issue 7 Pages
1323-1330
Published: 1978
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This investigation was conducted to observe changes in the fatty acid distributions of glycolipids (GL) and phospholipids (PL) in cotyledons of soybean seeds which were germinated either in the dark or the light at 28°C for 8 days. The GL isolated from the total lipids of cotyledons at different germinating stages were: acyl sterylglycoside (ASG), monogalactosyl diglyceride (MGD), digalactosyl diglyceride (DGD) and sulfolipid (SL). The PL isolated from the same total lipids as described above were: diphosphatidyl glycerol (DPG), phosphatidic acid (PA), phosphatidyl ethanolamine (PE), phosphatidyl glycerol (PG), phosphatidyl choline (PC) and phosphatidyl inositol (PI).
During germination of soybean seeds, the content of linoleic and linolenic acids in MGD or DGD was markedly higher than that of the other GL. The positional distribution of fatty acids in PE, PC and PI was shown in all PL, in which saturated fatty acids, especially palmitic acid, were highly concentrated in position 1 and unsaturated fatty acids, especially linoleic acid, mainly occupied position 2. A remarkable difference in the changing patterns of fatty acid composition, which depended on the germinating conditions tested, was observed between GL and PL. The changes in fatty acid composition of GL were more marked in the light-grown seedlings than in the dark-grown, whereas those of PL were more remarkable in the latter than in the former. Therefore, the positional distribution of fatty acids in PL was more evident in the light-grown seedlings than in the dark-grown ones.
These results suggest the metabolic fate of GL and PL in cotyledons of soybean seeds, probably owing to the differences in the two germinating conditions tested.
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Yuji ENOMOTO, Yoshio FURUTANI, Hiroshi NAGANAWA, Masa HAMADA, Tomio TA ...
1978 Volume 42 Issue 7 Pages
1331-1336
Published: 1978
Released on J-STAGE: November 27, 2008
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In the screening for inhibitors of cyclic adenosine-3', 5'-monophosphate phosphodiesterase, two compounds, PDE-I (C
18H
13N
3O
5) and PDE-II (C
14H
14N
2O
5), were isolated from culture filtrates of a Streptomyces. Concentrations for 50% inhibitions of PDE-I and PDE-II against the high
Km enzyme were 15 μM and 13 μM, and those against the low
Km enzyme were 65 μM and 130 μM, respectively. Production, isolation and characterization of these compounds are described.
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Hikaru NAKAMURA, Yuji ENOMOTO, Tomio TAKEUCHI, Hamao UMEZAWA, YOICHI I ...
1978 Volume 42 Issue 7 Pages
1337-1342
Published: 1978
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The structures of PDE-I and PDE-II, inhibitors of cyclic adenosine-3', 5'-monophosphate phosphodiesterase, were established as 3-carbamoyl-1, 2-dihydro-4-hydroxy-5-methoxy-3-H-pyrrolo[3, 2-e]indole-7-carboxylic acid and its 3-acetyl homologue, by X-ray diffraction study of monomethyl derivatives.
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Sawao MURAO, Naofumi MORITA, Yoshimaru YAMAMOTO, Kohei ODA
1978 Volume 42 Issue 7 Pages
1343-1349
Published: 1978
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The partial structure of glycopeptide moiety of new acid protease A isolated from
Scytalidium lignicolum ATCC 24568 was studied by Smith degradation, methylation and partial acetoly-sis techniques. The main product, glycopeptide V (GP-V), obtained by Pronase digestion was composed of mannose, glucosamine, asparagine, serine and glycine in an approximate molar ratio of 10:3:2:1:1, and a possible structure was proposed as follows:
_??_An Example of Possible Form
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Renzo HATTORI, Shigeru MURAKI, Toshio YOSHIDA
1978 Volume 42 Issue 7 Pages
1351-1356
Published: 1978
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The gardenia absolute was separated into basic, phenolic, acidic, lactonic and neutral fractions and analysed by using GC, combined GC-MS, IR and NMR. A total of 130 components was identified. Among them, the characteristic constituents responsible for the sweet-green odor of this flower were jasmin lactone, cis-3-hexenol, esters of cis-3-hexenol, cis-3-hexenoic acid and tiglic acid.
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Yasuo NAKADA, Shigeki MURAMATSU, Motoji ASAI, Shigeki OHNO, Yasuo YURA
1978 Volume 42 Issue 7 Pages
1357-1364
Published: 1978
Released on J-STAGE: November 27, 2008
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Indanyl 1- and 2-alcohols have been found to be a new entry in the list of “active alcohols” as the alcohol part of pyrethroids. Various substituted indanyl chrysanthemates were prepared and their insecticidal activity was tested on American cockroachs. These esters generally showed the substantial insecticidal activity, in which 4-allyl-l-indanyl ester 20c was more potent than allethrin. Examination of substituents on I-indanyl esters afforded some interesting results that methyl and allyl groups on the 4-position of the indanol nucleus were excellent and that no obvious electronic effects were observed.
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Yasuo NAKADA, Shigeki OHNO, Masafumi YOSHIMOTO, Yasuo YURA
1978 Volume 42 Issue 7 Pages
1365-1373
Published: 1978
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A variety of 4-substituted 1-indanyl chrysanthemates were prepared and their insecticidal activity was tested on American cockroachs. The activity of the chrysanthemates decreased in the following order: CH
2=CHCH
2>CH
3OCH
2_??_CH
3CH
2_??_HC≅CCH
2>PhCH
2, which was similar to that of p-substituted benzyl chrysanthemates against houseflies with the exception of the propargyl group. Formulation of the quantative structure-activity relationship by the Hansch's program indicated that Van der Waals interaction between the chemical substance and the macromolecular
in vivo play an important role in the 1-indanyl chrysanthemates.
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Hank J., LEEUWEN
1978 Volume 42 Issue 7 Pages
1375-1378
Published: 1978
Released on J-STAGE: November 27, 2008
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Specific antibodies were prepared against a bitter peptide, which had been previously isolated from tryptic hydrolysates of casein coprecipitate. The antibodies were immobilised by covalent attachment to Sepharose 4B and the resulting immunoadsorbents were able to remove bitterness from these hydrolysates. However, it was not possible to completely remove absorbed bitter peptide from the immunoadsorbents even when using strongly deforming solvents.
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Kazuo OKAMOTO, Takashi AKAZAWA
1978 Volume 42 Issue 7 Pages
1379-1384
Published: 1978
Released on J-STAGE: November 27, 2008
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During the germination of rice seed, multiple forms of amylase molecules are synthesized in the endosperm tissues as detectable by the isoelectric focusing on polyacrylamide gel. Among three major amylase components, two of them were purified to an apparent homo-geneity, and band A and C were identified to be α- and β-amylase, respectively. Some of their enzymic properties have been studied.
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Kunihiko GEKKO, Hidetoshi HARADA, Hajime NOGUCHI
1978 Volume 42 Issue 7 Pages
1385-1388
Published: 1978
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The interaction between diethylaminoethyldextran (DEAED) and bovine plasma albumin (BPA) was investigated at the pH range above the isoelectric point through the turbidity and the electrophoresis measurements. The mixture of DEAED-BPA showed a coacervate, the formation of which was sensitively affected by pH, ionic strength, temperature, and weight ratio of both compounds. The interaction between them appeared to be mainly electrostatic and to be enhanced by the hydrophobic interaction. The results obtained seems to support the localized charge distribution model on protein-polyion interaction.
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Kwang Yun CHO, Akira SAKURAI, Nobutaka TAKAHASHI, Saburo TAMURA
1978 Volume 42 Issue 7 Pages
1389-1396
Published: 1978
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Twelve new quarternary ammonium compounds were synthesized and their plant growth retarding activities were examined. Among the candidates, N, N, N-trimethyl-1-methyl-3-(3', 3', 5'-trimethylcyclohexyl)- and N, N, N-trimethyl-l-methyl-3-(3'', 3', 5', 5'-tetramethylcyclo-hexyl)-2-propenylammonium iodides were the most effective to suppress the growth of rice and cucumber seedlings, and their activities were far stronger than those of any growth retardants hitherto known.
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Masaki TERADA, Junichi MINAMI, Takehiko YAMAMOTO
1978 Volume 42 Issue 7 Pages
1397-1402
Published: 1978
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1. The SDS-disc electrophoretical analysis of wheat flour globulin revealed that upon incubation with “Kansui, ” a mixture of alkali carbonates, one (No. 9) of the components disappeared and another component (No. 11) increased significantly whose molecular weight was estimated to be more than one million. Incubation of flour globulin with “Kansui” resulted in decrease of SH groups of the globulin.
2. However, a mild reduction of the globulin incubated with “Kansui” caused reap-pearance of component No. 9 and a decrease of component No. 11.
3. Previous treatment of flour globulin with PCMB or AN completely inhibited such effects of “Kansui” as described above.
4. A fraction containing component No. 9 of flour globulin which was the main component responsible to polymerization by “Kansui, ” was isolated from “Kansui”-treated globulin by chromatography on columns of Sephadex G200 and Sepharose 4B.
5. The component protein thus isolated showed a typical phenomenon of reversibility in depolymerization to the original component corresponding to No. 9 component by chemical reduction and polymerization to a component corresponding to No. 9 at alkaline sides.
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Michio DOHI, Kei ARIMA
1978 Volume 42 Issue 7 Pages
1403-1410
Published: 1978
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Inositol deficiency caused the abnormalities of permeability of the cell envelope of the inositol exacting yeasts. In the case of
Schizosaccharomyces pombe, in which the marked leakage of cellular free-pool fraction was not detected, the uptake activity of glucose or methyl-glucoside decreased in inositol deficiency, especially in aerobic condition. Investigations on the compositions of lipids and fatty acids showed that the change in fatty acid composition was not so remarkable as that in phosphatides in inositol deficiency. One of the main causes of low transport activity may be due to the change in phosphatides, but not due to that in fatty acids, possibly. Intracellular contents of glucose was not less in inositol deficiency than in sufficiency. These results suggest that inositol deficiency caused the low activity of uptake, which might not be, however, the primary cause of low fermentative activity.
In the case of
Saccharomyces cerevisiae Ino
- mutant A-21-20, the similar results about permeability and lipid analyses were obtained in inositol deficiency.
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Keiichiro HIYAMA, Shigetaka OKADA
1978 Volume 42 Issue 7 Pages
1411-1417
Published: 1978
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Immunochemical identification certified the electrophoretically separable components of
Arthrobacter chondroitinase to be the multiple forms of chondroitinase AC.
The electrophoretic patterns of the enzyme preparations obtained at various cultivation stages showed that chondroitinase III is produced at first and the multiple forms of the chon-droitinase occur during cultivation of the strain. In mature culture, chondroitinase I was the main component among the multiple forms.
From the conversion experiments using the supernatant fluid of chondroitinase-free culture broth and uridine-5'-diphospho-D-glucose and using glycosidases from
Charonia lampas, chondroitinase I and chondroitinase II, respectively, seemed to arise from glucosylation of chondroitinase III and from de-glycosylation of chondroitinase III or I.
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Hiroshi FUKUI, Koichi KOSHIMIZU, Hiroshi EGAWA
1978 Volume 42 Issue 7 Pages
1419-1423
Published: 1978
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A new diterpene having antimicrobial activities was isolated from the leaves and twigs of
Chamaecyparis pisifera Endle (Cupressaceae), and named pisiferic acid. The structure was shown to be 12-hydroxyabieta-8, 11, 13-trien-20-oic acid.
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Itaro OGUNI, Keiko SUZUKI-NASU, Toshio MASUI
1978 Volume 42 Issue 7 Pages
1425-1426
Published: 1978
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Hisao SHIBATA, Sumio SHIMIZU
1978 Volume 42 Issue 7 Pages
1427-1428
Published: 1978
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Motoo ARAI, Eiko TSUCHIYA, Sawao MURAO
1978 Volume 42 Issue 7 Pages
1429-1430
Published: 1978
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Masato KATAYAMA, Shingo MARUMO
1978 Volume 42 Issue 7 Pages
1431-1433
Published: 1978
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Akiko FUJIWARA, Yoshihiko SHIOMI, Ken SUZUKI, Mitsuhiko FUJIWARA
1978 Volume 42 Issue 7 Pages
1435-1436
Published: 1978
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Takayuki ORITANI, Masami NANJYO, Masako FUJITA, Kyohei YAMASHITA
1978 Volume 42 Issue 7 Pages
1437-1438
Published: 1978
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Tadaaki KOMORI, Kiyohiko KUNUGITA, Kunio NAKAHARA, Hatsuo AOKI, Hirosh ...
1978 Volume 42 Issue 7 Pages
1439-1440
Published: 1978
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Jiunu-Shyong LAI, Shin-ichi OKUDA, Hajime TAKAHASHI
1978 Volume 42 Issue 7 Pages
1441-1442
Published: 1978
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Takeharu MASAKI, Keiji NAKAMURA, Masao ISONO, Masami SOEJIMA
1978 Volume 42 Issue 7 Pages
1443-1445
Published: 1978
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Yasutaka TAHARA, Koichiro SHINMOTO, Yuzo YAMADA, Keiji KONDO
1978 Volume 42 Issue 7 Pages
1447-1448
Published: 1978
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Setsuo TAKEUCHI, Mutsuyuki KOCHI, Kenji SAKAGUCHI, Kuniko NAKAGAWA, Ta ...
1978 Volume 42 Issue 7 Pages
1449-1451
Published: 1978
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Takayuki UWAJIMA, Osamu TERADA
1978 Volume 42 Issue 7 Pages
1453-1454
Published: 1978
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