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Shigeru MORIYAMA, Ryuichi MATSUNO, Kazuhiro NAKANISHI, Tadashi KAMIKUB ...
1980 Volume 44 Issue 12 Pages
2763-2771
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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The thermal stability of glucoamylase
* [α-1, 4:1, 6-glucan-4:6-glucohydrolase, EC 3.2.1.3] from
Rhizopus niveus has been investigated in the presence of dextran, DEAE-dextran, dextran sulfate, polyethylene glycol and Ficoll in a temperature range from 52.0 to 60.0°C in relation to kinetics. Protective effect of dextran against thermal inactivation of the enzyme was pronounced. Dextran, polyethylene glycol and Ficoll, being electroneutral polymers, showed distinct features. The protective effects of these polymers increased in the order: polyethylene glycol<Ficoll<dextran. The order was the same as that of affinities between glucoamylase and each of polymers determined by partition of glucoamylase in liquid-liquid two phase systems consisting of two of these polymers. On the other hand, glucoamylase with dextran sulfate exhibited an interesting phenomenon which was typical electrostatic interaction. The finding was explained in terms of electrical double layer.
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Sawao MURAO, Masaru KAMEDA, Toyokazu NISHINO, Yasutaro HAMAGISHI
1980 Volume 44 Issue 12 Pages
2773-2778
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Exogenous adenosine 5'-triphosphate 3'-diphosphate (pppApp) had interesting effects on the cell cycle of
B. subtilis IFO 3027. The growth rate was reduced by the addition of 1mM pppApp, and the vegetative cell form was significantly changed. Moreover, the sporulation frequency was increased by 100 times or more as compared with the culture without pppApp. The sporulation process seemed to be stimulated around
t0. pppGpp and ppGpp also showed the same effects as pppApp. Among these effects, depression in growth rate was restored by Mg
2+ and Ca
2+, and stimulation of sporulation was inhibited by Mg
2+, Ca
2+ and certain carbon sources, such as glucose and glycerol. On the other hand, casamino acids or monovalent cations showed no influence on the pppApp effects. pppApp was not incorporated into cells in experiments with radioactive pppApp.
View full abstract
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Masami KUSUNOSE, Toshihiro NAKANISHI, Noshi MINAMIURA, Takehiko YAMAMO ...
1980 Volume 44 Issue 12 Pages
2779-2784
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Nearly all the amino group-producing activity of the autolysate of cells of
Saccharomyces sake was recovered in the cell wall fraction obtained from the autolysis residue. The activity of the cell wall fraction was not lost even after repeated use.
The proteolytic activity of the fraction was not solubilized by incubation with detergents, disruption with cell mill or by freezing and thawing method, but was solubilized to some extent by incubation with a commercial yeast cell-lytic enzyme preparation.
The cell wall fraction hydrolysed casein to about 50%. When casein was previously treated with certain proteinases, more than 60% was digested. The activity of the fraction was significantly increased by the addition of Zn
2+ while it was decreased by several proteolytic enzyme inhibitors. The interesting fact was that in the presence of EDTA the cell wall fraction showed only carboxypeptidase-like activity, and attacked the oxidized insulin B-chain to release two amino acids from the carboxyl terminal in known order.
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Katsumi SHIBATA, Kazuo IWAI
1980 Volume 44 Issue 12 Pages
2785-2791
Published: 1980
Released on J-STAGE: November 27, 2008
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The pH profiles of crystalline quinolinate phosphoribosyltransferase (EC 2.4.2.19) activities from hog kidney and hog liver were found to vary according to 5-phosphoribosyl-1-pyrophosphate concentration. Both the kidney and liver enzyme activities were inhibited by 5-phosphoribosyl-1-pyrophosphate at an alkaline pH and physiological pH (pH 7.4) but not at an acidic pH. The inhibition by 5-phosphoribosyl-1-pyrophosphate was competitive for quinolinic acid. In the presence of 30% glycerol, both the kidney and liver enzyme activities were inhibited by 5-phosphoribosyl-1-pyrophosphate, even at an acidic pH.
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Shigeru EDA, Kunio KATO
1980 Volume 44 Issue 12 Pages
2793-2801
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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A pectin isolated from tobacco midrib contained residues of D-galacturonic acid (83.7%), L-rhamnose (2.2%), L-arabinose (2.4%) and n-galactose (11.2%) and small amounts of D-xylose and D-glucose. Methylation analysis of the pectin gave 2, 3, 5-tri- and 2, 3-di-
O-methyl-L-arabinose, 3, 4-di- and 3-
O-methyl-L-rhamnose and 2, 3, 6-tri-
O-methyl-D-galactose. Reduction with lithium aluminum hydride of the permethylated pectin gave mainly 2, 3-di-
O-methyl-D-galactose and the above methylated sugars. Partial acid hydrolysis gave homologous series of β-(1→4)-linked oligosaccharides up to pentaose of D-galactopyranosyl residues, and 2-
O-(α-D-galactopyranosyluronic acid)-L-rhamnose, and di- and tri-saccharides of α-(1→4)-linked D-galactopyranosyluronic acid residues.
These results suggest that the tobacco pectin has a backbone consisting of α-(1→4)-linked D-galactopyranosyluronic acid residues which is interspersed with 2-linked L-rhamnopyranosyl residues. Some of the L-rhamnopyranosyl residues carry substituents on C-4. The pectin has long chain moieties of β-(1→4)-linked D-galactopyranosyl residues.
View full abstract
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C. O. IKEDIOBI, G. O. C. ONYIA, C. E. ELUWAH
1980 Volume 44 Issue 12 Pages
2803-2809
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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The well-known alkaline picrate test for cyanide has been improved by incorporating an enzymatic step to make the assay much more specific and quantitative. The sensitivity or detection limit of this method was found to be 0.16 μg/cm
3 while the precision as indicated by the coefficient of variation was 3%. The method was, in addition, found to be rapid, simple, inexpensive and ideally suited for the analysis of large number of cassava tissues and products, such as may be encountered in cassava agronomy and breeding work or in industrial quality control laboratories. A trained operator working alone consistently analyzed at least 700 samples/day using this assay method.
View full abstract
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Yozo MACHIDA, Toru NAKANISHI
1980 Volume 44 Issue 12 Pages
2811-2815
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Uricase activity was found in
Enterobacter cloacae KY3074 grown on guanine, hypoxanthine, uric acid, and xanthine media. The enzyme was purified from cells grown on uric acid as a source of nitrogen. The purification procedure included ammonium sulfate fractionation, gel filtration on Sephadex G-150, and column chromatography on DEAE-cellulose and DEAE-Sephadex. The enzyme had a molecular weight of about 105, 000 and was specific for uric acid. The optimum pH was around 9.5, and the activity was inhibited by the presence of potassium cyanide, Ag
+ or Cu
2+. This uricase can be used for estimation of uric acid.
View full abstract
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Hajime YOSHIOKA, Shinsaku HAYASHIDA
1980 Volume 44 Issue 12 Pages
2817-2824
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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A thermophilic fungus,
Mucor miehei YH-10, isolated from manure was selected to produce thermostable β-glucosidase among 207 isolates. When
Mucor miehei YH-10 was grown on wheat bran medium, the maximal accumulation of thermostable β-glucosidase was obtained after 4 days at 50°C, The β-glucosidase had an optimal temperature of 60°C and retained 73% of original activity after heating at 95°C for 5min. The β-glucosidase was fractionated by Sephadex G-100 chromatography into two components during the purification steps. These components were further purified by consecutive column chromatographies until they were homogeneous on disc electrophoresis. One retained 56% of original activity after heating at 95°C for 5min, whereas the other was completely inactivated after heating at 80°C for 5min.
View full abstract
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Akira KUNINAKA, Kyuji ROKUGAWA, Hiroshi YOSHINO
1980 Volume 44 Issue 12 Pages
2825-2829
Published: 1980
Released on J-STAGE: November 27, 2008
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The following enzymes were detected in conidia of
Aspergillus oryzae var. No. 13 that were collected from a soy sauce factory: phosphatases acting nonspecifically on nucleotides; ribosidase(s) acting on adenosine, inosine, guanosine and xanthosine; and deaminases acting on guanine, adenosine, guanosine, cytidine, AMP and GMP. Most phosphatase activity was found to be bound firmly to the conidia. Thus, when a nucleotide solution was passed through a conidia-containing column, the corresponding nucleoside was recovered as a clear and colorless solution. The bound phosphatases were rather stable at pH 4 to 9 and at temperatures below 50°C. The optimum conditions for activity were at about 45°C and at pH about 5 and 8. When UMP•Na
2 solution was passed through a column containing 5 g of the conidia, about 200mg of substrate was hydrolyzed per 1 hr. Conidia of molds belonging to the genus
Aspergillus may be industrially employed as naturally immobilized phosphatases.
View full abstract
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Nobuji NAKATANI, Reiko INATANI, Hidetsugu FUWA
1980 Volume 44 Issue 12 Pages
2831-2836
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Two phenolic amides were isolated from the fruits of white pepper (
Piper nigrum L.) and identified to be
N-
trans-feruloyl tyramine (2a) and
N-5-(4-hydroxyphenyl)-2
E, 4
E-pentadienoyl piperidine (6a) on the basis of chemical and specrtal evidence. Both compounds were synthesized.
View full abstract
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Yoshinori KOBAYASHI, Hideo UEYAMA, Koki HORIKOSHI
1980 Volume 44 Issue 12 Pages
2837-2841
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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An alkalophilic bacterium, strain No. 150-1, which had NAD-dependent sugar dehydrogenase activities on maltose (NAD-MalDH) and D-glucose (NAD-GlcDH), was isolated from soil. This microorganism was identified to be in a strain of the genus
Corynebacterium. The bacterium grew to similar degrees at Na
2CO
3 concentrations from 0 to 0.5%. NAD-MalDH and NAD-GlcDH were not inducible types. Soybean casein was the most effective nitrogen source for enzyme production. Activity staining of these two dehydrogense on polyacrylamide gel showed that these activities were derived from two different proteins.The cell free extract did not contain NADP-dependent maltose dehydrogenase.
View full abstract
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Goro KATO, Yoshiharu MARUYAMA, Michinori NAKAMURA
1980 Volume 44 Issue 12 Pages
2843-2855
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Phase-contrast and fluorescence microscopy observations showed that pea symbiont
R. leguminosarum adsorbed to pea root hairs, but non-symbiont rhizobial strains only adsorbed to a small extent.
14C-labeled cells were used to assay the number of rhizobial cells adsorbed to a pea root. Capsular polysaccharides or lipopolysaccharides obtained from
R. leguminosarum specifically inhibited the adsorption of
14C-
R. leguminosarum cells to a pea root and specifically adsorbed to pea root hairs. Also, they reacted specifically with pea seed lectins. These results suggest that capsular polysaccharides or lipopolysaccharides play an important role in host-specific adsorption. The interaction between the polysaccharides and pea lectins could be the key to determining host specificity in the infection process of
Rhizobiumpea symbiosis.
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Hisakazu YAMANE, Jun SUGAWARA, Yasushi SUZUKI, Etsuko SHIMAMURA, Nobut ...
1980 Volume 44 Issue 12 Pages
2857-2864
Published: 1980
Released on J-STAGE: November 27, 2008
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Jasmonic acid (JA)-related compounds were synthesized, and their inhibitory activities on rice seedling growth were investigated. Three functions (C-1 CH
2COOH or CH
2COOCH
3, C-2 (
Z)-2'-pentenyl or
n-pentyl and C-3 ketone or hydroxyl) were essential for exhibiting inhibitory activity in this series of compounds. A dihydro-JA-related compound, 4-acetylnonanoic acid, also showed inhibitory activity similar to JA.
View full abstract
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Osato MIYAWAKI, Kozo NAKAMURA, Toshimasa YANO
1980 Volume 44 Issue 12 Pages
2865-2870
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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The permeability of nylon microcapsule membrane was measured by two methods: (1) by NH
4Cl tracer permeation method and (2) by the overall reaction behaviour of urcase immobilized in microcapsule. The latter method gave higher values of permeability. It was possible to calculate permeability and activity simultaneously by the application of the least squares method to the results of overall microencapsulated urease reaction. Permeability varied with protein used in microencapsulation, which seemed to show the participation of proteins in the copolymerization reaction of nylon. The cut-off molecular weight of capsule membrane was measured to be 1, 000_??_10, 000 by permeation experiments using tracers of different molecular weights. These membrane characteristics were successfully applied to molecular sieving chromatography using a column packed with microcapsules.
View full abstract
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Hideo ETOH, Kazuo INA, Masanobu IGUCHI
1980 Volume 44 Issue 12 Pages
2871-2876
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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New syntheses of edulans and theaspiranes are described. The key step involves the cyclization of 4-(2', 6', 6'-trimethyl-2'-hydroxy-3'-cyclohexen-l'-yl)-butan-2-ol (
7) and 4-(2', 6', 6'-trimethyl-l', 2'-dihydroxy-3'-cyclohexen-l'-yl)-butan-2-ol (
8) derived from α-ionone.
The diol (
7) and triol (
8) were cyclized with acetic acid into the corresponding tetrahydropyran derivatives [
9a,
9b (dihydroedulan I and II, respectively), and
10a,
10b]; the latter two compounds were dehydrated to edulan I and II (
13a,
13b). On treatment with tosyl chloride in pyridine, however, triol (
8) gave spiro-compounds (
11a,
11b, and
12); (
11a and
11b) were easily converted to
cis- and
trans-theaspirone by oxidation and 5, 6-
erythro-6-hydroxy-dihydrotheaspiranes (
15a and
15b) by reduction.
View full abstract
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Hisao TAKAYANAGI, Chikao NISHINO
1980 Volume 44 Issue 12 Pages
2877-2883
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Configurational isomers of verbenyl carboxylic acid and its derivatives were synthesized from (+)-α-pinene
via a key intermediate, phenyl verbenyl sulfone. Configurations of the compounds were determined from stereochemical features in the reactions and the
1H- and
13C-NMR spectral properties.
View full abstract
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Sadaaki IIBUCHI, Toshimasa YANO
1980 Volume 44 Issue 12 Pages
2885-2892
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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The performance of a multi-stage bubble separator was studied with the emphasis on a dimensionless term composed of the backmixing rate between adjacent stages, surface production rate, and two parameters of Langmuir adsorption isotherm. The dimensionless term represents the efficiency of separation in the multi-stage bubble separator, and it can be a scaling-up factor. The number of stages necessary to obtain a given effluent concentration can be obtained by modification of McCabe-Thiel method.
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Shuhei NAKAJIMA, Kazuyoshi KAWAZU
1980 Volume 44 Issue 12 Pages
2893-2899
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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An ovicide and a larval growth inhibitor against
Drosophila melanogaster were isolated from leaves of
Eupatorium japonicum. The ovicide was identified as coumarin. The structure of the larval growth inhibitor, a new naturally occurring guaianolide named euponin, was elucidated on the basis of spectral and chemical evidence. The inhibitory effects of these agents on the egg and larva were also described.
View full abstract
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Shouji OHMORI, Hiroshi MASAI, Kei ARIMA, Teruhiko BEPPU
1980 Volume 44 Issue 12 Pages
2901-2906
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Industrial vinegar production by submerged acetic acid fermentation has been carried out using
Acetobacter strains at about 30°C. To obtain strains suitable for acetic acid fermentation at higher temperature, about 1, 100 strains of acetic acid bacteria were isolated from vinegar mash, soils in vinegar factories and fruits, and their activities to oxidize ethanol at high temperature were examined. One of these strains, No. 1023, identified as
Acetobacter aceti, retained full activity to produce acetic acid in continuous submerged culture at 35°C and produced 45% of activity at 38°C, while the usual strain of
A. aceti completely lost its activity at 35°C. Thus the use of this strain may reduce the cooling costs of industrial vinegar production.
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Hideshi FUJIWAKE, Tetsuya SUZUKI, Shinzaburo OKA, Kazuo IWAI
1980 Volume 44 Issue 12 Pages
2907-2912
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Cell-free extracts of placenta of
Capsicum annuum var.
annuum cv. Karayatsubusa catalyzed the formation of capsaicinoid from vanillylamine and 7-methylocatnoyl CoA (iso-C-
9:0-CoA), 8-methylnonanoyl CoA (iso-C-
10:0-CoA), 8-methyl-6-nonenoyl CoA (iso-C-
10:1-CoA), or 9-methyldecanoyl CoA (iso-C-
11:0-CoA). Iso-type fatty acid-activating reaction required CoA, ATP and Mg
++ as cofactors. Among acyl-CoAs examined, iso-C-
9:0-CoA was used most effectively as acyl donor for capsaicinoid formation. 5-methylhexanoyl CoA (iso-C-
7:0-CoA), 6-methylheptanoyl CoA (iso-C-
8:0-CoA), 10-methylundecanoyl CoA (iso-C-
12:0-CoA) and decanoyl CoA (
n-C-
10:0-CoA) were not used as acyl donor. When equivalent amounts of iso-C-
9:0-CoA, iso-C-
10:0-CoA, iso-C-
10:1-COA, and iso-C-
11:0-CoA were added together as acyl donors, nordihydrocapsaicin was the major product, occupying more than 60% of the total capsaicinoid formed. The optimum pH for capsaicinoid formation was around 9.0, when iso-C-
10:0-CoA was used as acyl donor.
View full abstract
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Shigeo YOSHIDA, Yuichiro NAGAO, Nobutaka TAKAHASHI
1980 Volume 44 Issue 12 Pages
2913-2920
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Varoius piericidin analogues (PS-I, -II and -III in Fig. 2) were synthesized from three 4-acetoxy-6-formylpyridines by Wittig reaction to determine the structure-activity relationships. New type inhibitors, 5-alkenyl-2, 3-dimethoxy-4-hydroxy-6-methylpyridines (PS-IV) were synthesized by intramolecular cyclization.
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Shigeo YOSHIDA, Yuichiro NAGAO, Akio WATANABE, Nobutaka TAKAHASHI
1980 Volume 44 Issue 12 Pages
2921-2924
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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The study describes the inhibitory activity of natural piericidins and related compounds, including synthetic analogues, to the electron transport system of mitochondria. Consideration of the structure-activity relationships led to the proposing of a structural unit I that was essential to inhibitory activity.
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Binh Trong NGUYEN, Tohru KODAMA, Yasuji MINODA
1980 Volume 44 Issue 12 Pages
2925-2930
Published: 1980
Released on J-STAGE: November 27, 2008
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Gram negative hydrogen bacterium
Pseudomonas hydrogenovora was found to excrete an anthrone-H
2SO
4 positive viscous polysaccharide. About 12g/liter of the polysaccharide was produced autotrophically on gaseous hydrogen at the stationary phase of growth. Biosynthesis of the polysaccharide occurred under nitrogen-deficient condition. Its elementary composition was C:39.29%, H:6.23%, O:49.67%, N:0.21% and ash 4.6%. The polysaccharide contained galactose, glucose, mannose and rhamnose as its main components. The polysaccharide had anti-tobacco mosaic virus and anti-tumor activities.
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Kyohei YAMASHITA, Akihiro TAKAIWA, Hitomi NAKADA
1980 Volume 44 Issue 12 Pages
2931-2935
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Debutenoyl-aspertetronin A was synthesized from T-valerolactone-Y-carboxylic acid (
4)
via 2, 5-dihydro-3-hydroxy-2-methyl-5-oxo-2-furanpropanoic acid. Starting from (-)-(
S)-
4-(+)-(
S)-5-hexyl-4-hydroxy-5-methyl-2(5
H)-furanone (
19) s as synthesized, and by comparison of its optical rotation with that of an authentic sample it was proved that aspertetronin A had (
R) configuration, and gregatin A had (
S) configuration at their respective chiral carbon.
View full abstract
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Hiroshi MATSUZAWA, Sadamitsu ASOH, Takahisa OHTA, Shigeo TAMAKI, Michi ...
1980 Volume 44 Issue 12 Pages
2937-2941
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Escherichia coli rodA mutant AOS151 grows as round cells at 30 and 42°C (H. Matsuzawa, K. Hayakawa, T. Sato, and K. Imahori,
J. Bacteriol., 115, 436_??_442 (1973)). The mutant was found to be resistant to mecillinam at both temperatures.
lip+ transductants were prepared by PI phage transduction
via strain AOS151, the cotransduction frequency of round morphology (rod
-) at 42°C with the
lip gene being about 90%. At 42°C all 54 rod
- transductants tested were resistant to mecillinam. At 30°C all but two of these rod
- (at 42°C)-type transductants were rod-shaped, and all were sensitive to mecillinam; the two strains grew as ovoid cells. The original
rodA mutant AOS151 probably involves an additional mutation(s), that expresses the round cell shape at lower temperature, whereas the
rodA51 mutation alone seems to result in temperature-sensitive expression of round cell morphology and mecillinam resistance.
rodA mutant cells cultured at either 30 or 42°C had wild-type penicillin-binding protein 2, judging from penicillin-binding activity, electrophoretic mobility, and thermosensitivity.
View full abstract
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Chitoshi HATANAKA, Yoshiaki KOBARA
1980 Volume 44 Issue 12 Pages
2943-2949
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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Nelson's arsenomolybdate, the chromogenic reagent in Somogyi-Nelson method, was replaced by Folin-Ciocalteu phenol reagent. The major object was to remove the toxic arsenic compounds from the color reaction system. The color-producing ability of the phenol reagent was considerably lower than that of Nelson's reagent. However, the modified method was favorably comparable to Somogyi-Nelson method in simplicity, reproducibility and stability of color development. The error in both the modified and Somogyi-Nelson method could be reduced to about one fourth by adding sodium benzoate (final concentration, about 0.5%) to the test solutions.
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Sadao SAKAMURA, Yoshihiko TERAYAMA, Satomi KAWAKATSU, Akitami ICHIHARA ...
1980 Volume 44 Issue 12 Pages
2951-2954
Published: 1980
Released on J-STAGE: November 27, 2008
JOURNAL
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New three conjugated serotonins were isolated from safflower meal (
carthamus tinctorius L.).On the basis of spectral properties and chemical evidence, their structures were determined as N-feruloylserotonin (4),
N-(
p-coumaroyl)serotonin (5) and
N-(
p-coumaroyl)serotonin mono-β-D-glucopyranoside (6). The known compounds, 2-hydroxyarctiin (2), matairesinol mono-β-D-glucoside (1) and acacetin (3) were also isolated and identified.
View full abstract
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Kimiyasu ISOBE, Yoshiki TANI, Hideaki YAMADA
1980 Volume 44 Issue 12 Pages
2955-2960
Published: 1980
Released on J-STAGE: November 27, 2008
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Polyamine oxidase from
Penicillium chrysogenum oxidized spermine rapidly and spermidine slightly at pH 7.5. The apparent Km values for spermine and spermidine were calculated to be 2.25×10
-5M and 9.54×10
-6M, respectively. The relative maximum velocities for spermine and spermidine were 3.37×10
-3M (H
2O
2) per min per mg of protein and 2.08×10
-4M (H
2O
2) per min per mg of protein, respectively. Spermine oxidation of the enzyme was competitively inhibited by spermidine and putrescine. The apparent
Ki values by spermidine and putrescine were calculated to be 3.00×10
-5M and 1.80×10
-3M, respectively. On the other hand, polyamine oxidase from
Aspergillus terreus rapidly oxidized both spermidine and spermine at pH 6.5. The apparent Km values for spermidine and spermine were 1.20×10
-6M and 5.37×10
-7M, respectively. The relative maximum velocities for spermidine and spermine were 1.55×10
-2M (H
2O
2) per min per mg of protein and 6.20×10
-3M (H
2O
2) per min per mg of protein, respectively.
Differential determination of spermine and spermidine was carried out using the two enzymes. The initial rate was assayed with
Penicillium enzyme and the end point was measured afte addition of
Aspergillus enzyme. Small amounts of polyamines (25 to 200 nmol of spermine and 25 to 250 nmol of spermidine) were assayed by solving two simultaneous equations obtained from the rate assay method and the end point assay method. The calculated values were in close agreement with those obtained by an amino-acid analyzer.
View full abstract
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Lyang-Ja LEE, Satomi KINOSHITA, Hidehiko KUMAGAI, Tatsurokuro TOCHIKUR ...
1980 Volume 44 Issue 12 Pages
2961-2966
Published: 1980
Released on J-STAGE: November 27, 2008
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Crystalline galactokinase was obtained in good yield from
Bifidobacterium bifidum grown on galactose medium. This preparation moved as a single protein band in analytical disc electrophoresis and sedimented as a single symmetrical peak under ultracentrifugation. The enzyme exhibited similar physicochemical properties to galactokinase purified from glucosegrown cells of
B. bifidum. The enzyme has a molecular weight of 47, 000. Only galactose and ATP were effective as substrate.
Km values, optimal pH, cation requirement, inhibition by SH-reagent, heat stability and product inhibition were also investigated.
View full abstract
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F. MACRÌ, A. VIANELLO
1980 Volume 44 Issue 12 Pages
2967-2970
Published: 1980
Released on J-STAGE: November 27, 2008
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The photodynamic activity of three compounds structurally related to cercosporin (
iso-cercosporin, phleichrome,
iso-phleichrome) and rubellin, a pigment possessing an anthraquinone-like structure, was studied. As previously reported for cercosporin,
iso-cercosporin, phleichrome, iso-phleichrome and rubellin, when irradiated by an incandescent lamp, induced oxygen uptake and malondialdehyde (MDA) formation in pea stem and rat liver mitochondria and microsomes. All these compounds were also capable of inhibiting MDA formation induced by the peroxidizing system ascorbate-FeSO
4. Circumstantial evidence suggests that the compounds, as shown for cercosporin, induce a lipoperoxidative degradation of the polyunsaturated fatty acids of cell membranes when irradiated by light, but at the same time, they are able to lower the lipoperoxidation induced by ferrous ions.
View full abstract
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Junji MORITA, Naoki KASHIMURA, Tohru KOMANO
1980 Volume 44 Issue 12 Pages
2971-2978
Published: 1980
Released on J-STAGE: November 27, 2008
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Autoxidizable synthetic polysaccharides prepared by polycondensation of reducing aldose or ketose in dimethyl sulfoxide containing pohsphorus pentaoxide [
Polymer, 13, 190 (1972)] inactivated phage φX174. Another autoxidizable polysaccharides obtained by oxidation of natural glucans with the same oxidant also inactivated φX174. The φX174 inactivation was due to strand scission of viral DNA in the virion. The inactivation reaction was stimulated by Cu
2+ and inhibited by EDTA, superoxide dismutase, catalase and several radical scavengers.These results suggest that oxygen radicals produced during autoxidation of polysaccharides are responsible for φX174 inactivation.
View full abstract
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Koji IKURA, Takashi KOMETANI, Ryuzo SASAKI, Hideo CHIBA
1980 Volume 44 Issue 12 Pages
2979-2984
Published: 1980
Released on J-STAGE: November 27, 2008
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Transglutaminase catalyzes the formation of intermolecular and intramolecular ε-(γ-glutamyl)lysyl crosslinks in proteins. The study here examined the substrate effectiveness of soybean 7S and 11S proteins in the intermolecular-crosslinking reaction catalyzed by guinea pig liver transglutaminase.
Both 7S and 11S proteins could act as the substrate for the transglutaminase reaction.The reaction with 11S protein was faster than that of 7S protein. Analyses of the reaction products by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that three main subunit groups of 7S protein and two acidic subunit groups of 11S protein were polymerized through the formation of intermolecular crosslinks by transglutaminase. Interestingly enough, no intermolecular crosslink was formed between the basic subunits of 11S protein. The possible significance of the intermolecular crosslinking catalyzed by transglutaminase is discussed, including the use of this enzyme reaction to improve the properties of food protein.
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R. KAMAL, S. C. JAIN
1980 Volume 44 Issue 12 Pages
2985
Published: 1980
Released on J-STAGE: November 27, 2008
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Masao HORIBA, Hajimu KITAHARA, Seiya YAMAMOTO, Naobumi ÔI
1980 Volume 44 Issue 12 Pages
2987-2988
Published: 1980
Released on J-STAGE: November 27, 2008
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Masao HORIBA, Hajimu KITAHARA, Seiya YAMAMOTO, Naobumi ÔI
1980 Volume 44 Issue 12 Pages
2989-2990
Published: 1980
Released on J-STAGE: November 27, 2008
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Yoshinori KOBAYASHI, Koki HORIKOSHI
1980 Volume 44 Issue 12 Pages
2991-2993
Published: 1980
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N. K. ROY, B. LALLJEE, S. BEDI
1980 Volume 44 Issue 12 Pages
2995-2997
Published: 1980
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Hideo ETOH, Kazuo INA, Masanobu IGUCHI
1980 Volume 44 Issue 12 Pages
2999-3000
Published: 1980
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Hiroshi SUGIYAMA, Taichi USUI
1980 Volume 44 Issue 12 Pages
3001-3002
Published: 1980
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Kimikazu IWAMI, Kyoden YASUMOTO
1980 Volume 44 Issue 12 Pages
3003-3004
Published: 1980
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Masaaki YASUDA, Seizen TOYAMA, Robert R. RANDO, Nobuyoshi ESAKI, Katsu ...
1980 Volume 44 Issue 12 Pages
3005-3006
Published: 1980
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Chimaki HOSHINO, Kenji MORI
1980 Volume 44 Issue 12 Pages
3007-3009
Published: 1980
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Hideo KUBO, Kunio OISHI, Ko AIDA
1980 Volume 44 Issue 12 Pages
3011-3013
Published: 1980
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Nobuhiro MORI, Bunsei KAWAKAMI, Kimiaki HYAKUTOME, Yoshiki TANI, Hidea ...
1980 Volume 44 Issue 12 Pages
3015-3016
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Tsutomu YAMAGUCHI
1980 Volume 44 Issue 12 Pages
3017-3018
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Makiko TAKAGI, Saburo FUNAHASHI, Keiichi OHTA, Toshio NAKABAYASHI
1980 Volume 44 Issue 12 Pages
3019-3020
Published: 1980
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Hiroomi WATABE, Eiji HAYASE, Tadahiko ANDO
1980 Volume 44 Issue 12 Pages
3021-3023
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Daisuke YOSHIDA, Hitoshi OKAMOTO
1980 Volume 44 Issue 12 Pages
3025-3027
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Akira ISOGAI, Akinori SUZUKI, Shizuo HIGASHIKAWA, Shimpei KUYAMA, Sabu ...
1980 Volume 44 Issue 12 Pages
3029-3031
Published: 1980
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Akira ISOGAI, Akinori SUZUKI, Shizuo HIGASHIKAWA, Shimpei KUYAMA, Sabu ...
1980 Volume 44 Issue 12 Pages
3033-3035
Published: 1980
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