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Makio MORITA
1981 Volume 45 Issue 11 Pages
2403-2407
Published: 1981
Released on J-STAGE: March 27, 2006
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Non-metal-requiring catalysts in recovered methyl linolenate from an autoxidizing sample were unstable on contact with silicic acid and the activity varied widely with the way of recovery, countercurrent extraction and column chromatography under different conditions. After reduction with stannous chloride, a substance was found which was characteristically present in the products derived from the recovered methyl linolenate high in the catalytic activity. It was tentatively identified by MS, IR, OsO
4 oxidation-IO
4- oxidation, and LiAlH
4 reduction-IO
4- oxidation as methyl 9, 13-dihydroxy-10-epoxy-15-octadecenoate.
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Atsushi HATTORI, Keijiro ISHIBASHI, Sadamasa MINATO
1981 Volume 45 Issue 11 Pages
2409-2416
Published: 1981
Released on J-STAGE: March 27, 2006
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Dextranase (EC 3.2.1.11) produced by
Chaetomium gracile was purified by sequential chromatographies on CM-and DEAE-cellulose columns, and two active fractions, CD-I and CDII, were isolated in electrophoretically pure states. The former fraction was obtained in a crystalline state. The estimated molecular weights were 77, 000 for CD-I and 71, 000 for CD-II, and their isoelectric points were 6.2 and 5.7, respectively. Both active fractions contained a sugar moiety (4.5%). Their amino acid compositions were determined. They were very similar to each other in enzymatic properties: The optimum pH was at around 5.5, and they were stable between pH 5.5 and 1 1.0, and at temperatures lower than 55°C. They were typical endodextranases, but their maximal degrees of dextran hydrolysis reached 55% as glucose.
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Akira YOKOTA, Ken-ichi SASAJIMA
1981 Volume 45 Issue 11 Pages
2417-2423
Published: 1981
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Sporulation marker enzymes, D-glucose dehydrogenase and L-alanine dehydrogenase were synthesized derepressively by vegetative cells of a
Bacillus species mutant which was isolated as an improved D-ribose producer. It was also elucidated by electron microscopy that no morphological change concerning sporulation took place during the course of the enzyme syntheses in the mutant strain. The presence of Mn
2+ and Ca
2+ in the mediumwas necessary for the morphological development of sporulation even in the mutant strain. The mechanismof derepressed enzyme syntheses is discussed in relation to regulation of sporulation.
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Hajime YOSHIOKA, Nobuo NAGATO, Surina CHAVANICH, Naline NILUBOL, Shins ...
1981 Volume 45 Issue 11 Pages
2425-2432
Published: 1981
Released on J-STAGE: March 27, 2006
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Thermostable xylanase from
Talaromyces byssochlamydoides YH-50 was fractionated into three components, tentatively named X-a, X-b-I and X-b-II during the purification steps. X-a, X-b-I and X-b-II were further purified by consecutive column chromatographies until found to be in homogeneous states on disc electrophoresis. X-a, X-b-I and X-b-II contained 36.6%, 31.5% and 14.2% carbohydrate residues, respectively. The carbohydrate residues were glucose, mannose and fucose. X-a, X-b-I and X-b-II were optimally active at 7075°C and pH 4.55.5. X-a, X-b-I and X-b-II retained 65, 54 and 30%, respectively, of the original activity after heating at 95°C for 5 min. The activities of X-a, X-b-I and X-b-II were considerably inhibited by HgCl
2 and KMnO
4. The hydrolysis products from xylan with X-a were xylose, arabinose, glucose, xylobiose and other xylooligosaccharides, whereas the hydrolysis products from xylan with X-b-I and X-b-II were xylose and xylobiose.
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Masato TERASAWA, Joji TAKAHASHI
1981 Volume 45 Issue 11 Pages
2433-2441
Published: 1981
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Metabolic pathways for the oxidation of 1-tetradecene by
Candida lipolytica were investigated and compared with those for
Candida tropicalis in order to elucidate the difference in the productivity of citrates reported in the previous paper. No difference was found in the pathways for the initial stage of oxidation of 1-tetradecene between the two strains, indicating that the difference in the productivity ofcitrates is not attributable to the metabolic pathways. The metabolic rate of 1- tetradecene with
C. lipolytica was found to be muchlower than that with
C. tropicalis. The production of citrates was much enhanced in the presence of surfactants which were knownto be stimulative for microbial metabolism of hydrocarbons and 1115 g/liters was attained on the 6th day of cultivation.
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Dong Ki PARK, Junji TERAO, Setsuro MATSUSHITA
1981 Volume 45 Issue 11 Pages
2443-2448
Published: 1981
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Trilinoleoylglycerol (TL) was autoxidized at 37°C in the dark. Monohydroperoxides (MHP) obtained from the oxidized products were analyzed by high performance liquid chromatography (HPLC). Several peaks which appeared in the chromatogram were identified by infrared (IR), gas chromatography mass spectrometry (GC-MS) and enzymatic hydrolysis. Some positional and geometrical isomers of their hydroperoxy fatty acid components were separated using both absorption and reversed phase systems. Furthermore, 1-hydroperoxylinoleoyl-2, 3-dilinoleoylglycerol and 1, 3-dilinoleoyl-2-hydroperoxylinoleoylglycerol were partly separated by HPLC using an absorption system. MHP obtained from autoxidized corn oil, safflower oil and soybean oil were separated into some peaks by HPLC, although resolution into the individual isomers was incomplete. Whenoxidized oils were subjected to HPLC analysis directly, a linear relationship was observed between the peak areas of MHP and peroxide value in the range of 1050 meq/kg.
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Tadashi YOSHIMOTO, Daisuke TSURU
1981 Volume 45 Issue 11 Pages
2449-2455
Published: 1981
Released on J-STAGE: March 27, 2006
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An arylamidase was purified from
Flavobacterium meningosepticum by a series of chromatographies on CM-cellulose, DEAE-Sephadex A-50 and Sephadex G-150. The purified enzyme appeared homogeneous on SDS-gel electrophoresis. The molecular weight of the enzyme was estimated to be more than 500, 000 dalton by using a column of Sepharose 4B and to be 62, 000 when checked by SDS-gel electrophoresis. The enzyme was most active at pH 7.5 toward Leu-β-naphthylamide (Leu-β-NA). It catalyzed the hydrolysis of not only various amino acid-β- naphthylamides but also some peptides, but the hydrolysis rate of the latter substrates was quite low. Cys-di-β-naphthylamide was split by this enzyme at an optimal pH of 6.2. Incubation of oxytocin with the enzyme resulted in a decrease in the biological activity, indicating that this arylamidase possesses an oxytocinase (cystyl aminopeptidase)-like activity.
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Effect of Mitomycin C on Extracellular DNA Production
Toshio HARA, Seinosuke UEDA
1981 Volume 45 Issue 11 Pages
2457-2461
Published: 1981
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The mechanism of excretion of DNA was investigated using strain KYU-1. This strain produced a considerable amount of DNA extracellularly (7.8 mg/ml), and the amount of extracellular DNA per ml of the culture was scores of times more compared to that of intracellular DNA per ml of the culture. DNA synthesis was repressed by the addition of inhibitors (nalidixic acid, 5-fluoro uracil or mitomycin C), their inhibition rates were 35 to 54%. With the addition of 100 μg/ml of mitomycin C at the middle of the logarithmic growth phase, only 0.89 mg of extracellular DNA per ml of the culture was obtained, the inhibition rate was 95%. However, the amount of organic phosphate synthesized in the culture was almost the sameno matter whether with or without an inhibitor.
Furthermore, the excretion of DNA from the cell surface of strain KYU-1 was observed with on electron microscope and the DNA molecules excreted were found to be double-stranded.
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Fumihiko SATO, Naruhiko NAKAGAWA, Tadashi TANIO, Yasuyuki YAMADA
1981 Volume 45 Issue 11 Pages
2463-2467
Published: 1981
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The photoautotrophic culture medium for
Cytisus scoparius Link cells was improved. Linsmaier and Skoog mediumwas modified by adding a 4-fold concentration of phosphate and lowering the auxin concentration (α-naphthaleneacetic acid 10
-6 M). Concentrations of all the inorganic elements except phosphate in the original basal mediumwere adequate for the photoautotrophic growth of
Cytisus cells. Ahigh concentration of cytokinin compensated for the inhibition of growth caused by a high concentration of auxin.
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Wen-Chang CHIANG, Sadaaki IIBUCHI, Toshimasa YANO
1981 Volume 45 Issue 11 Pages
2469-2473
Published: 1981
Released on J-STAGE: March 27, 2006
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An unexpected phenomenon named "reverse enrichment" was observed, in which, contrary to the normal behaviour, isoelectric hemoglobin coagula concentrated in the bottom stage of a multistage bubble separator. The phenomenon was observed when the hemoglobin particles were highly concentrated, the bubble diameter was larger than one third of the hole diameter of the partition plate, and the superficial air velocity at the hole was high. Transient and final behaviours of the multi-stage bubble separator with the reverse enrichment were analyzed by assuming the rejection of particles from upward thin liquid streams.
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Yoshitake TANAKA, Yoko Takahashi, Rokurou MASUMA, Yuzuru IWAI, Haruo T ...
1981 Volume 45 Issue 11 Pages
2475-2481
Published: 1981
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Stimulation of leucomycin production with
Streptomyces kitasatoensis by magnesium phosphate (MgP), and a concomitant decrease of ammonium ion concentration in the culture supernatant have been reported from this laboratory. Cultural characteristics of the MgPsupplemented culture were further examined in relation to the stimulation of leucomycin production. When the leucomycin titer increased 4- to 5-fold in the presence of MgP, mycelial growth level and assimilation rate of a major carbon source (glycerol) increased about 2-fold. MgPgrown mycelia were not appreciably different in shape or in carbon and nitrogen contents from the control mycelia. Magnesium and phosphate ions were released from MgP into the medium, while the addition of magnesium or phosphate salts to the control medium was rather inhibitory for leucomycin production. The pH values of MgP-supplemented cultures were lower than those of the control culture. When modifications of the basal media and culture conditions were done so as to permit the pH-change almost identical with that of the control media, the leucomycin titer was still higher in the presence of MgP than in its absence. When uric acid, an insoluble compound, was used as the sole nitrogen source, the leucomycin production increased to a high level even in the absence of MgP, which was comparable to that seen when MgP was added to the ammonium lactatecontaining medium. The ammonium ion concentration in the culture supernatant of the uric acidcontaining medium was maintained at a considerably low level. While in the ammonium lactatecontaining medium, leucomycin production was low and ammonium ion concentration remained at a high level. Thus, it was demonstrated that the stimulation of leucomycin production by MgP is related to the lowering of ammonium ion level, but not to the other factors examined such as growth level, carbon or phosphate regulation and pH-effect.
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Tetsuya MASUTANI, Junkichi IWASA, Itsuo ICHIMOTO, Hiroo UEDA
1981 Volume 45 Issue 11 Pages
2483-2489
Published: 1981
Released on J-STAGE: March 27, 2006
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Rhodojaponin-III and 10, 14-epoxy-10-deoxy grayanotoxin-III were derived from grayanotoxin-I and -III, respectively. Preparation of 13 new derivatives from α-dihydro grayanotoxin-II was discussed. Some of them showed higher physiological activity than that of natural grayanotoxins.
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Makoto SHIMIZU, Takamoto KAMIYA, Kunio YAMAUCHI
1981 Volume 45 Issue 11 Pages
2491-2496
Published: 1981
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Whey proteins adsorbed on fat globule surfaces during emulsification with coconut oil at pH 3 - 9 were examined. The amount of proteins adsorbed on the fat surface was dependent on the pH during emulsification. At any pH examined here, however, tightly-adsorbed proteins which were not extracted from the fat surface with urea or guanidine-HCl were 2 - 3 mg/m
2. Marked selectivities in the adsorption of individual whey proteins were observed at any pH. No correlation between the adsorbabilities and the surface hydrophobicities of individual whey proteins was observed. Whey proteins adsorbed on the emulsified fat were much more easily digested with proteases compared to the native whey proteins, indicating that conformational changes of whey proteins occurred at the fat surface. The results suggested that conformational properties, such as flexibility of the structure, of whey proteins are important in the adsorption and possibly affect their emulsifying ability.
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Shoji SHIMA, Heiichi SAKAI
1981 Volume 45 Issue 11 Pages
2497-2502
Published: 1981
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As a result of screening for a Dragendorff-positive substance of microbial origin, L-lysine homopolymer (25-30 residues) with alpha and epsilon linkages was obtained from the culture filtrate of an actinomycete identified as
Streptomyces albulus. Various cultural conditions for the favorable production of the lysine polymer were studied. The decline of pH during the fermentation process was an essential condition for the accumulation of lysine polymer. The addition of proline had a considerable effect.
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Shoji SHIMA, Heiichi SAKAI
1981 Volume 45 Issue 11 Pages
2503-2508
Published: 1981
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The chemical nature of the Dragendorff-positive substance produced by
Streptomyces albulus was investigated. The substance was found to be a homopolymerof L-lysine connected with 25 - 30 residues in an isopeptide linkage. The ε-poly-L-lysine is a unique natural substance not described before now.
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Toshio SUGURO, Wendell L. ROELOFS, Kenji MORI
1981 Volume 45 Issue 11 Pages
2509-2514
Published: 1981
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Both (
E)- and (Z)-isomers of (±)-6-isopropyl-3, 9-dimethyl-5, 8-decadienyl acetate were stereoselectively synthesized. The former was shown to be the racemate of the sex pheromone of the yellow scale,
Aonidiella citrina.
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Satoru MASUDA, Shigefumi KUWAHARA, Toshio SUGURO, Kenji MORI
1981 Volume 45 Issue 11 Pages
2515-2520
Published: 1981
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The (
S)-enantiomer of the sex pheromone of the yellow scale (
Aonidiella citrina), (
S,
E)-6-isopropyl-3, 9-dimethyl-5, 8-decadienyl acetate, was stereoselectively synthesized from (
R)-(+)-citronellic acid.
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Yasuo MIYASHITA, Kenji MORI
1981 Volume 45 Issue 11 Pages
2521-2526
Published: 1981
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Both the enantiomers ( - 96% e.e.) of (2Z, 6Z)-2, 6-nonadien-4-olide, a possible pheromone of
Aphomia gularis, were synthesized by resolving an acetylenic intermediate.
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Atsushi SUZUKI, Takakazu YAMADA, Makoto SAITO, Yoshinobu NONAMI
1981 Volume 45 Issue 11 Pages
2527-2533
Published: 1981
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Reconstitution of Z-disks extracted with a low ionic strength solution by incubating proteins released from myofibrils by Ca
2+ -activated factor (CAF) with Z-disk-extracted fiber bundles was successful, but the physiological activity of the fiber bundles with reconstituted Z-disks has not been studied.
To elucidate the relationships between the structure of Z-disks and physiological activity of myofibrils, the contractilities of intact, Z-disk-extracted and -reconstituted fiber bundles were studied by optical and electron microscopy. Intact fiber bundles that contracted immediately after the addition of contracting solution (Mg
2+ -ATP solution) showed the disappearance of the I-band and the formation of a double overlap of thin filaments in the center of the A-band. The contractility of the fiber bundles decreased with the progress of extraction of Z-disks, and formation of the double overlap of thin filaments in the center of the A-band was not observed. The fiber bundles with Z-disks reconstituted recovered the mechanism of contraction similar to that of the intact fiber bundles. When the Z-disks were removed by CAF instead of the low ionic strength solution, the reconstitution of Z-disks was not successful and therefore the contractility of the fiber bundles was not recovered.
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Atsushi SUZUKI, Makoto SAITO, Akihiro OKITANI, Yoshinobu NONAMi
1981 Volume 45 Issue 11 Pages
2535-2542
Published: 1981
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The materials released from myofibrils by Ca
2+ -activated factor (CAF) were fractionated by
using a combination of Sepharose 6B and Bio-gel A 50m columns. To investigate the exact constituents of Z-disks on the basis of success or failure of reconstitution of Z-disks, reconstitution of Z-disks by incubating individual fractions with Z-disk-extracted fiber bundles was carried out, and the proteins in each fraction were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis.
From the electron micrographs of the reconstituted Z-disks, SDS-polyacrylamide gel electrophoretic analysis and amino acid analysis of the isolated proteins from the gels, it was clear that the proteins required for the reconstitution of the Z-disk, in other words, the principal constituents of the Z-disk, were Z-nin, a new high molecular weight protein having a subunit molecular weight of 300, 000-400, 000 dalton mentioned in this report, α-actinin and tropomyosin.
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Hidetsugu NAKAZAWA, Hidehiko KUMAGAI, Hideaki YAMADA
1981 Volume 45 Issue 11 Pages
2543-2552
Published: 1981
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An aromatic L-amino acid decarboxylase was crystallized from the cell free extract of
Micrococcus percitreus. The purification procedure included protamine sulfate treatment, ammonium sulfate fractionation, DEAE-Sephadex column chromatography and Sephadex G-200 filtration. Crystals were obtained from a solution of the purified enzyme by the addition of ammonium sulfate.
The crystalline enzyme preparation was homogeneous as judged by ultracentrifugation and SDS-polyacrylamide gel electrophoresis. The molecular weight was determined to be approximately 101, 000. The enzyme was evidently composed of two identical subunits of a molecular weight of 48, 000. The enzyme catalyzed the stoichiometric conversion of L-tryptophan to tryptamine and CO
2 in the presence of pyridoxal phosphate. The optimum pH was 9.0 for the conversion.
The
Km value and the maximum velocity of L-tryptophan decarboxylation were 2.4 x 10
-3 M and 44 μmol/min/mg of protein, respectively. This enzyme also catalyzed decarboxylation of 5-hydroxy-L-tryptophan, L-phenylalanine, L-tyrosine, 3, 4-dihydroxy-L-phenylalanine, L-kynurenine and their α-methyl amino acid derivatives.
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Hidetsugu NAKAZAWA, Katsumi AJISAKA, Masatsune KAINOSHO, Seiji SAWADA, ...
1981 Volume 45 Issue 11 Pages
2553-2557
Published: 1981
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The stereochemistry of the decarboxylation reaction catalyzed by an aromatic L-amino acid decarboxylase, purified from
Micrococcus percitreus, was studied using stereospecifically deuterium labelled phenylalanine (Phe). The
1H NMR spectrum of [1, 2-
2H
2]-β-phenethylamine enzymatically derived from (2
S, 3
R)-[3-
2H]-Phe in
2H
2O was compared with that of [l-
2H]- β-phenethylamine from unlabelled Phe in
2H
2O. The results clearly indicate that the decarboxylation reaction of this enzyme proceeds exclusively through a course in which the configuration at C-2 of Phe is retained.
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Fumitaka HAYASE, Hiromichi KATO
1981 Volume 45 Issue 11 Pages
2559-2567
Published: 1981
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The nondialyzable melanoidins prepared from glucose-glycine (I), xylose-glycine (II) and glucose-β-alanine (III) reaction systems were pyrolyzed at 350°C for 0.5 2 hr and the volatile pyrolyzates were investigated. To trap the volatile compounds, cold trapping and Tenax GC trapping methods were used. Identification of these compounds was made by gas chromatographymass spectrometry using a glass capillary column. The volatile components of I and II were qualitatively similar to each other. Major components of volatile pyrolyzates of I and II were identified as methyl acetate, five furans, toluene, two pyrroles, two pyridines and acetic acid. The major volatile components of III were methyl acetate, five furans, two 2-cyclopentenones, pyrrole, two pyridines and acetic acid.
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Shinsuke TANABE, Ryo TATSUKAWA, Hiroyuki TANAKA, Kohji MARUYAMA, Nobuy ...
1981 Volume 45 Issue 11 Pages
2569-2578
Published: 1981
Released on J-STAGE: March 27, 2006
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As the first of a series of studies on the ecology and bioaccumulation of striped dolphins (
Stenella coeruleoalba), whole-body burdens of chlorinated hydrocarbons such as PCBs, DDT compounds, HCH isomers and HCB in striped dolphins were estimated by detailed biometric measurements of organs and tissues, and determination of chlorinated hydrocarbon concentrations in them.
The majority (over 90%) of the chlorinated hydrocarbon burden in the bodies of striped dolphins, excluding the fetus, was in their blubber which comprised on average 17.3% of the body weight. So, it is justifiable to think that the residual amounts of chlorinated hydrocarbons in their blubber are usable in place of those in their whole-bodies. This enabled us to easily determine the chlorinated hydrocarbon burdens and concentrations on a whole-body basis from the concentrations in the blubber.
The approach presented here for estimating the whole-body burdens of residual chemicals should be useful for studies on the dynamics and fates of chlorinated hydrocarbons in food chains, especially in large-sized marine mammals.
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Kojiro WADA, Shingo MARUMO
1981 Volume 45 Issue 11 Pages
2579-2585
Published: 1981
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The analogues of brassinolide, 15, 19-23, 29 and 30, were synthesized
via key intermediate 5 from stigmasterol. Compound 15 which has the same functional groups as brassinolide on rings A and B showed one tenth of the activity of brassinolide in the lamina inclination test on rice seedlings. However, other analogues with modified ring A were poorly active, and an analogue with modified ring B, 6-oxa-ketone 30, showed only one hundredth of the activity of its 7-oxa-ketone isomer, 29, suggesting that the 2α, 3α-dihydroxy-7-oxa-6-ketone moiety is one of the requisites for the biological activity.
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Koji IKURA, Masaaki YOSHIKAWA, Ryuzo SASAKI, Hideo CHIBA
1981 Volume 45 Issue 11 Pages
2587-2592
Published: 1981
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Transglutaminase catalyzes the formation of ε-(ν-glutamyl)lysine crosslinks and the substitution of a variety of primary amines for the ν-carboxamide groups of protein-bound glutaminyl residues. This study examined the feasibility of the use of the transglutaminase reaction for fortifying food proteins by the covalent attachment of limiting essential amino acids.
In the reactions using L-methionine ethyl ester as a substrate, the methionine content of α
s1 and β-caseins, and soybean 7S and 11S proteins increased to 200, 150, 240 and 350% of the starting materials, respectively. With wheat gluten, incorporation of L-lysine was tested and a 5.1-fold increase in lysine content was observed. These results suggest that transglutaminase could be a useful tool for improving the amino acid composition of food proteins.
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Teruhiko YOSHIHARA, Katsuyoshi YAMAGUCHI, Seiji TAKAMATSU, Sadao SAKAM ...
1981 Volume 45 Issue 11 Pages
2593-2598
Published: 1981
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Two new phenolic amides, grossamide (7) and
N-
cis-feruloyl tyramine (2), have been isolated from the roots of bell pepper (
Capsicum annuum var.
grossum) together with
p-aminobenzaldehyde (1),
N-
trans-feruloyl tyramine (3),
N-
trans-
p-coumaroyl tyramine (4),
N-
trans-feruloyl octopamine (5), and
N-
trans-
p-coumroyl octopamine (6).
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Sawao MURAO, Akira GOTO, Yoshihiro MATSUI, Kunio OHYAMA, Motoo ARAI
1981 Volume 45 Issue 11 Pages
2599-2604
Published: 1981
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A screening test was carried out to obtain microbes which produce hog pancreatic α-amylase inhibitor and a new inhibitor was found in culture broth of an actinomycete, strain YM-25.This inhibitor was designated as Haim, an abbreviation for hog pancreatic α-amylase inhibitor from a microbe. The determined morphological and physiological properties of strain YM-25 led to the conclusion that the microorganism was
Streptomyces griseosporeus.
When the microorganism was aerobically cultured at 30°C in a jar fermentor containing the most suitable medium for growth which consisted of 5% glycerol, 0.5% polypepton, 0.2% meat extract, 0.1% yeast extract, 0.4% Na
2HPO
4- 12H
2O, 0.1% KH
2PO
4, and 0.05% MgSO
4 - 7H
2O (pH 7.3), the highest activity of Haim was obtained on 23-26 hr cultivation.
Haim had specific inhibitory activities against animal α-amylases but not against microbial and plant α-amylases.
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Takeshi OGITA, Haruo SETO, Noboru OTAKE, Hiroshi YONEHARA
1981 Volume 45 Issue 11 Pages
2605-2611
Published: 1981
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The structures of minor congeners of the detoxin complex,
viz., detoxins E
1, C
1, C
2, C
3, B
1, B
3 and A
1, have been established on the basis of degradative and spectroscopic evidences.
It is of interest that the detoxin B group contains 5-deoxydetoxinine in place of detoxinine, and that a change of the terminal amino acid residue from L-valine to L-isoleucine was first recognized in detoxin E
1.
The structure-activity relationship of the detoxin compounds is discussed with comparison of their antagonistic activities against blasticidin S.
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Masanobu MUNEKATA, Gakuzo TAMURA
1981 Volume 45 Issue 11 Pages
2613-2618
Published: 1981
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Two diketopiperazines have been isolated from the culture broth of a soil Actinomycetales,
Streptomyces sp. strain ML1532. They were cyclo(L-Val-L-Pro) and cyclo(L-Tyr-L-Pro). The selective cytotoxicity of cyclo(L-Tyr-L-Pro) was slightly higher than that of cyclo(L-Val-L-Pro) against SV40-transformed cells.
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Yuji ODA, Kazutaka MIYATAKE, Yoshihisa NAKANO, Shozaburo KITAOKA
1981 Volume 45 Issue 11 Pages
2619-2621
Published: 1981
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Yoshihisa OZOE, Kazuo MOCHIDA, Morifusa ETO
1981 Volume 45 Issue 11 Pages
2623-2625
Published: 1981
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Akira KIMURA, Kenji ARIMA, Kousaku MURATA
1981 Volume 45 Issue 11 Pages
2627-2629
Published: 1981
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Yoshimitsu YAMAZAKI, Hidekatsu MAEDA
1981 Volume 45 Issue 11 Pages
2631-2632
Published: 1981
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Makoto NISHIDA, Suong-Be HYEON, Akinori SUZUKI
1981 Volume 45 Issue 11 Pages
2633-2635
Published: 1981
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Makoto NISHIDA, Suong-Be HYEON, Akira ISOGAI, Akinori SUZUKI
1981 Volume 45 Issue 11 Pages
2637-2638
Published: 1981
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Yoshinobu NAOSHIMA, Tomowa MORITA, Shoji WAKABAYASHI, Shuichi HAYASHI
1981 Volume 45 Issue 11 Pages
2639-2640
Published: 1981
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-
Takahisa SUZUKI
1981 Volume 45 Issue 11 Pages
2641-2643
Published: 1981
Released on J-STAGE: March 27, 2006
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Takashi SHINOHARA, Masazumi WATANABE
1981 Volume 45 Issue 11 Pages
2645-2651
Published: 1981
Released on J-STAGE: March 27, 2006
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Yasuo KIMURA, Takashi HAMASAKI, Hiromitsu NAKAJIMA
1981 Volume 45 Issue 11 Pages
2653-2654
Published: 1981
Released on J-STAGE: March 27, 2006
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Takeyoshi SUGIYAMA, Junji MASAKI, Hiroshi SASADA, Kyohei YAMASHITA
1981 Volume 45 Issue 11 Pages
2655-2658
Published: 1981
Released on J-STAGE: March 27, 2006
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Unusual five 4-ethyl-fatty acids were estimated as main constituents of sebaceous gland secretion of male goat which showed the releaser pheromone activity against the estrous female. These acids exist in both free state and ester form. The structures were confirmed by synthesis. Among the five acids, 4-ethyloctanoic acid showed the strong goaty odor at low concentration.
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Stanislaw GWIAZDA, Kyoko SAIO
1981 Volume 45 Issue 11 Pages
2659-2660
Published: 1981
Released on J-STAGE: March 27, 2006
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Yoshitake TANAKA, Satoshi OMURA, Kazumi ARAKI, Kiyoshi NAKAYAMA
1981 Volume 45 Issue 11 Pages
2661-2664
Published: 1981
Released on J-STAGE: March 27, 2006
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Naofumi MORITA, Keiichi INOUE, Masanosuke TAKAGI
1981 Volume 45 Issue 11 Pages
2665-2668
Published: 1981
Released on J-STAGE: March 27, 2006
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Osamu KIRINO, Kunihiko FURUZAWA, Hiroshi MATSUMOTO, Naganori HINO, Aki ...
1981 Volume 45 Issue 11 Pages
2669-2670
Published: 1981
Released on J-STAGE: March 27, 2006
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Yuzo YAMADA, Joji SASAKI, Chin Fei HOU, Yasutaka TAHARA, Mary MIRANDA, ...
1981 Volume 45 Issue 11 Pages
2671-2673
Published: 1981
Released on J-STAGE: March 27, 2006
JOURNAL
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