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Kunihiko WATANABE, Kousaku MURATA, Akira KIMURA
1986 Volume 50 Issue 8 Pages
1925-1930
Published: 1986
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γ-Glutamylcysteine synthetase was purified from
E. coli B. The enzyme had a molecular weight of 5.5 × 10
4 and required only magnesium ion for activity. The optimal pH and temperature for reaction were 8.5 and 45°C, respectively. The
Km values for L-glutamate, L-cysteine, and ATP were 0.50, 0.09, and 0.01 HIM, respectively. GTP and UTP were also used as energy sources. The enzyme activity was inhibited by phosphate anions and by various sulfhydryl reagents. Unlike the enzyme from mammalian tissues, the
E. coli B enzyme was not inhibited by α-alkyl analogues of methionine. The enzyme was feedback inhibited by reduced glutathione, although oxidized glutathione had no inhibitory effect.
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Ali ASGHAR, Jun-Ichiro MORITA, Kunihiko SAMEJIMA, Tsutomu YASUI
1986 Volume 50 Issue 8 Pages
1931-1940
Published: 1986
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The influence of under-nutrition (sub-maintenance feeding) and
ad libitum feeding on the deposition of proteins in different Subcellular Sarcoplasmic fractions of red (tonic) and white (phasic) muscles of growing broilers was investigated. The relative concentration of overall Sarcoplasmic proteins was lesser in red than in white muscles from
ad libitum fed broilers. The content of mitochondrial proteins was slightly more and that of lysosomal and microsomal proteins and of true soluble proteins was lesser in red than in white muscles. Besides, the relative amount of some specific molecular weight proteins in each Subcellular fractions differed by more than 50% between red and white muscle.
There was also conspicuous differences in the influence of under-nutrition on the proteins in red and white muscles. Some reduction in mitochondrial, lysosomal, microsomal, and soluble protein content occurred only in white muscle, whereas little change was found in Subcellular fractions in red muscle from underfed broilers. The relative amount of some proteins in each Subcellular fraction of both muscles remained unaffected, and that of others either decreased or increased more than 20 to 50% due to nutritional stress.
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Ali ASGHAR, Jun-Ichiro MORITA, Kunihiko SAMEJIMA, Tsutomu YASUI
1986 Volume 50 Issue 8 Pages
1941-1949
Published: 1986
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The influence of under-nutrition (sub-maintenance feeding) and
ad libitum feeding on myofibrillar and connective tissue (collagen-like) proteins of red (leg) and white (breast) muscle of growing broilers was studied. The content of 0.3 M KCl-extractable myofibrillar proteins was lower, and that of 6M guanidine-HCl- and 1% Triton X-100-soluble proteins and connective tissue proteins was higher in red than in white muscle of
ad libitum fed broilers. The relative amount of some specific proteins in 0.3 M KCl-, 0.6 M KI-, and Triton X-100-extracts differed by more than 20 to 50% between red and white muscle.
Under-nutrition caused some decrease in KCl-extractable proteins of red muscle, and an increase in the connective tissue content of red muscle and in KI-, guanidine-HCl-extractable proteins, and connective tissue of white muscle. SDS-polyacrylamide gel electrophoretograms of 0.3 M KCl extracts showed that the relative concentration of 95 K protein (α-actinin) in both muscles and 59 K protein in red muscle from underfed broilers was lower than that from controls, whereas the relative content of 200 K (myosin heavy chain), 160K, and 43 K (actin) proteins was rather high in both muscles from underfed broilers. Noticeable changes in the levels of some amino acids of connective tissue were also observed in red and white muscle as a result of under-nutrition.
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Fumitaka HAYASE, Seon Bong KIM, Hiromichi KATO
1986 Volume 50 Issue 8 Pages
1951-1957
Published: 1986
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Nondialyzable melanoidins, which were prepared from a glucose-glycine system at 95°C for 7, 26 and 44 hr, were subjected to NMR analyses before and after ozonolysis. On
13C CP-MAS NMR, signals in the regions of 10-50, 60-75, 105-115 (I), 130-140 (II), 170-180 and 190-205 (III) ppm were observed for the melanoidins. The signals of I, II and III completely disappeared on ozone-treatment. These findings indicate that the melanoidins were composed mainly of saturated and aliphatic carbons. The
13C NMR spectra of glucose (1-
13C)-glycine melanoidins showed that C-1 carbons originating from glucose in the melanoidins were distributed at the positions of aliphatic alkyl methyl (IV), saturated, unsaturated or aromatic and carbonyl (V) carbons. The signals of IV and V disappeared on ozone-treatment. Studies using glycine-2-
13C revealed that most of the glycine is incorporated into the melanoidins. On
15N CP-MAS NMR, weak broad signals in the region of 0 - 70 ppm and strong signals in the region of 70-120 ppm with a shoulder at 120-170 ppm (VI) were observed for the melanoidins. The signals of VI disappeared on ozonetreatment. The main linkage of nitrogen (70-120ppm) was remarkably shifted from that of the original glycine and was determined to be a conjugated enamine linkage.
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Tateki HAYASHI, Sadako MASE, Mitsuo NAMKI
1986 Volume 50 Issue 8 Pages
1959-1964
Published: 1986
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Formation of a product easily converted to methylglyoxal on TLC with silica gel was observed in an early stage of the reaction mixture of sugar with an alkylamine or amino acid. NMR spectra of the ether extract of reaction mixtures indicated that methylglyoxal dialkylimine was produced mainly at an early stage of the reaction of glucose with alkylamine, and was assumed to change to methylglyoxal on the TLC. The C
3 imine production in the
t-butylamine system was apparently little and slow compared to that in the normal alkylamine system. A large, rapid production of C
3 imine was also observed in the system of the Amadori product and
n-butylamine. These results suggested that the C
3 formation in the system with normal alkylamine may occur mainly
via a newly proposed mechanism, though the t-butylamine system may possibly produce it according to the scheme proposed by Hodge.
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Tateki HAYASHI, Mitsuo NAMIKI
1986 Volume 50 Issue 8 Pages
1965-1970
Published: 1986
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Glycolaldehyde and methylglyoxal, both model compounds structurally related to potential C
2 and C
3 sugar fragments, showed extremely high reaction rates in browning with β-alanine compared to the usual reducing sugars and even to such active intermediate products of aminocarbonyl reaction as the Amadori product and osones. Production of C
2 and C
3 sugar fragments in a glucose-β-alanine system was negligible in acidic conditions, but increased with pH in a manner parallel to the increase in browning and also to the N/C ratio of the melanoidins. These results indicated that the proposed new pathway of browning, involving sugar fragmentation, is very important in the initial stages of browning in the Maillard reaction of neutral or alkaline solutions.
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Toshihiko OSAWA, Harue ISHIBASHI, Mitsuo NAMDCI, Tsuneo KADA, Keiichi ...
1986 Volume 50 Issue 8 Pages
1971-1977
Published: 1986
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The desmutagenic activity of various food components on C-nitro mutagens formed by the nitrite/sorbic acid reaction was assayed and several vegetable juices were found to be effective for eliminating the mutagenicity of the nitrite/sorbic acid system. Especially, the desmutagenic activity of pumpkin juice was investigated, and ascorbic acid, cysteine and other reducing compounds were found to be responsible for desmutagenic actions on 1, 4-dinitro-2-methyl pyrrole, the main mutagen formed by the reaction of sorbic acid with sodium nitrite, by reduction of the conjugated C-nitro group to a C-amino group.
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Shoichi TAKAO, Hiroshi SASAKI, Kazuhiko KUROSAWA, Masatoshi TANIDA, Yo ...
1986 Volume 50 Issue 8 Pages
1979-1987
Published: 1986
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Corticium rolfsii AHU 9627, which we isolated from a tomato stem, is one of the most promising producers of a raw starch saccharifying enzyme. The effects of the cultural conditions and medium components on the enzyme production were investigated. The enzyme production was improved by increasing both the concentrations of carbon sources and organic nutrients in the medium. Under the optimum cultural conditions, the enzyme activity of the culture supernatant against raw starch reached a maximum after 8-days incubation at 27°C and the activity reached 80 units per ml (when determined at 40°C and pH 4.0). The optimal pH and temperature for the enzyme reaction were 4.0 and 65°C, respectively. The saccharifying reaction was scarcely inhibited even with a high substrate concentration, and raw starch was rapidly hydrolyzed into glucose.
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Naotoshi MATSUDOMI, Atsushi TANAKA, Akio KATO, Kunihiko KOBAYASHI
1986 Volume 50 Issue 8 Pages
1989-1994
Published: 1986
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The functional properties of gluten obtained by treating with chymotrypsin at alkali pH were investigated. The gluten was treated by chymotrypsin at pH 10.0 and 20°C, and was found to be deamidated to a state that was scarcely subject to proteolysis by chymotrypsin. The degree of deamidation of the gluten reached about 25% by this treatment for 2 hr. The functional properties of the gluten thus obtained were investigated in regard to deamidation. The enzymatically deamidated gluten greatly improved such functional properties as solubility and emulsifying ability. In particular, the solubility of the treated gluten was remarkably high in the pH range of 5 to 8, in which native gluten is insoluble. It was apparent that the improvement in functional properties of gluten was mainly due to the deamidation induced by treating with chymotrypsin at pH 10.0 and 20°C.
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Kohei ODA, Sawao MURAO
1986 Volume 50 Issue 8 Pages
1995-2001
Published: 1986
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S. lignicolum acid proteinases A-l, A-2, and B (S-PI-insensitive) were examined for the structure of the active sites by the method of Nakatani using PAD as a probe. We attempted to screen for substances that release zinc(II)-PAD from the complex of zinc(II)-PAD-S-PI-insensitive acid proteinases. Angiotensin I released zinc(II)-PAD from the complex as well as from the complex of S-PI-sensitive acid proteinases. Angiotensin I is a good substrate for these enzymes regardless of S-PI-sensitivity. These results suggest that the two carboxyl groups capable of binding to zinc(II)-PAD are at the active site regions of
S. lignicolum enzymes and that they are involved in their catalytic actions.
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Nobuhisa MOROOKA, Takashi TATSUNO, Hiroshi TSUNODA, Kimiko KOBAYASHI, ...
1986 Volume 50 Issue 8 Pages
2003-2007
Published: 1986
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A phytotoxin, isopiraarane diterpene γ-lactol, 6α, 7β, 9α-trihydroxy-8(14), 15-isopimaradiene20, 6-γ-lactone, was isolated from the culture filtrate of the fungus
Phomopsis sp., which was obtained from the interior part of the trunk of most wilting Japanese red pines,
Pinns densiflora Sieb. et Zucc. The stereostructure was established by X-ray crystal analysis and spectroanalysis. This γ-lactol was identical with LL-S491, a fermentation product of the fungus
Aspergillus chevalieri. The cytotoxic activity of the γ-lactol isopimarane was the same as pentachlorophenol at 150 ppm on cell cultures from Japanese red pine buds.
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Takamitsu YORIFUJI, Kenzo KOIKE, Takao SAKURAI, Ken-ichi YOKOYAMA
1986 Volume 50 Issue 8 Pages
2009-2016
Published: 1986
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L-Arginine induced two NAD
+-specific dehydrogenases acting on 4-guanidinobutyraldehyde (GBAL) in
Pseudomonas putida ATCC 12633. One was identified as 4-aminobutyraldehyde dehydrogenase because it was induced by putrescine also and was active toward 4-aminobutyraldehyde (ABAL). The other was specific for GBAL and was identified as GBAL dehydrogenase. We purified both enzymes. The molecular weight and the subunit weight of ABAL dehydrogenase were 240, 000 and 57, 000, respectively. GBAL dehydrogenase had a molecular weight of 107, 000 and a subunit weight of 57, 000. ABAL dehydrogenase was most active toward ABAL at pH 8.0 and toward GBAL at pH 8.5 to 9.5. This enzyme dehydrogenated ABAL (100%), GBAL (130%), 5-aminovaleraldehyde (100%), valeraldehyde (66%), butyraldehyde (66%), and propionaldehyde (49%). The apparent
Km values for ABAL and NAD
+ were estimated to be 0.26 and 0.05 mM, respectively. GBAL dehydrogenase exhibited the optimum pH to be 9.0 and the high substrate specificity for GBAL. The apparent
Km values for GBAL and NAD
+ were 0.03 and 0.14 mm, respectively. This enzyme was readily inactivated in the absence of 2-mercaptoethanol, and NAD
+ and 2-mercaptoethanol were required for full reactivation of the inactivated enzyme.
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Yasumasa KUWAHARA, Yasuhiko YONEKAWA, Takashi KAMKIHARA, Takahisa SUZU ...
1986 Volume 50 Issue 8 Pages
2017-2024
Published: 1986
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Since all positional isomers of methyl undecenoates were available and also an undecenoate sex pheromone of the varied carpet beetle, identification of the double bond position of the pheromone was attempted by comparing the mass spectra of the isomers with that of the pheromone, using a newly devised fitness index. The index (%) was calculated by dividing the absolute difference between the intensity ratio of every fragment ion-pair of an isomer and the corresponding ratio of the pheromone by the latter for each isomer. All the index values were summed up for each isomer and averaged to determine the similarity to the pheromone. The smallest average among the isomers indicates the best fit to the pheromone.
The results of a comparison based on all commonly observable ions (a total of 63 ions above
m/z 41, with intensities more than 0.1%) and 17 selected ion groups (above
m/z 55 or
m/z 59 with intensities of more than 0.1%, 1%, 3%, 5%, 10% or 20%, respectively, and above
m/z 41 with intensities more than 1%, 3%, 5%, 10% or 20%, respectively) indicated that methyl 5-undecenoate was the most probable candidate for the pheromone, except two ion groups (above
m/z 41 with intensities of more than 10% and 20%, respectively). Furthermore, statistically significant results were obtained for all groups above
m/z 41 with intensities up to 5% and groups above
m/z 55 and 59 with intensities more than 10%. Therefore the present study verified the feasibility of using mass spectrometry for the identification of the double bond position of a pheromone.
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Katsumi SHIBATA, Kazumi TANAKA, Kiku MURATA
1986 Volume 50 Issue 8 Pages
2025-2032
Published: 1986
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The nutritional efficiency of quinolinic acid as a substitute for nicotinic acid was investigated using weanling rats of the Sprague Dawley strain (3-weeks old) fed a nicotinic acid-free, tryptophan-limited diet containing various amounts of nicotinic acid or quinolinic acid. Judging from the growth response, food efficiency ratio, levels of NAD activity in the blood, liver, brain and upper small intestine, and urinary excretion of niacin we have concluded that exogenous quinolinic acid is approximately 1/9 as active as nicotinic acid. As many foods contain quinolinic acid, dietary quinolinic acid cannot be ignored from the standpoint of tryptophan and nicotinic acid replacement.
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Takanori KASAI, Keiko NISHINO, Kei SONOYAMA, Shuhachi KIRIYAMA
1986 Volume 50 Issue 8 Pages
2033-2037
Published: 1986
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Absorption rates of four diastereoisomers of dimethionine from rat small intestine were measured as the first step of an attempt to find methionine derivatives which are absorbed much more slowly than L-methionine from rat small intestine. The absorption rates of L, D- and D, L-dimethionine were nearly the same and roughly one half of that of the L, L-diastereoisomer. The absorption rate of D, D-dimethionine was far slower than those of the other three diastereoisomers (about one tenth of L, L-dimethionine). The absorption rate of γ-L-glutamyl-L-methionine was nearly the same as those of L, D- and D, L-dimethionine.
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Shigeru HAYAKAWA, Ryo NAKAMURA
1986 Volume 50 Issue 8 Pages
2039-2046
Published: 1986
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Mapping super-simplex optimization (MSO) and the response surface method (RSM) were performed to determine the optimum conditions for giving the maximum gel breaking strength (BS) of heat-induced lysozyme gel.
A maximum BS of 138.8dyn/cm
2 was obtained at pH 7.24, NaCl 46.9mM, dithiothreitol 7.0 mM and a heating time 12.7min at 80°C by MSO. However, a low predicted maximum value of 22.7dyn/cm
2 was obtained by using RSM at conditions close to those for the local optimum in MSO after iterative steps. A multiple regression, analysis showed the significant contribution of NaCl (p<0.01) and dithiothreitol (p<0.05) to gel formation.
It is suggested that an ion shielding effect by salt and disruption of the disulfide bonds, causing exposure of the hydrophobic regions followed by interchanges between sulfhydryl and disulfide groups, were responsible for the heat-induced gelation of lysozyme.
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Katsuji WATANABE, Eiji TANIGUCHI
1986 Volume 50 Issue 8 Pages
2047-2052
Published: 1986
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Several derivatives of 2-(1', 3'-dioxo-3'-phenylpropyl)phenylacetic acid, a homolog of the auxin transport inhibitor 2-(1', 3'-dioxo-3'-phenylpropyl)benzoic acid (CPD), were synthesized. The phenylacetic acids with a heterocyclic ring such as isoxazole between the two phenyl groups inhibited the growth of lettuce, rice, and buckwheat. They prevented the active movement of auxin within the bean segments and abolished the root geotropic response of lettuce seedlings, as well as auxin transport-inhibiting benzoic acids. These phenylacetic acids may be intrinsic inhibitors of auxin transport.
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Shozo FUJIOKA, Akira SAKURAI, Naotsugu SUGIMOTO, Isomaro YAMAGUCHI, No ...
1986 Volume 50 Issue 8 Pages
2053-2059
Published: 1986
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Efforts were made to isolate flower-inhibitory substances from extracts of the short-day plant
Lemna paucicostata 381.
Lemna paucicostata 151, which was used in the bioassay, exhibits poor flowering in response to the photoperiod, but flowers profusely in response to benzoic acid. Therefore, only those substances that inhibit benzoic acid-induced flowering were studied. Several fractions obtained by silica gel column chromatography exhibited flower-inhibitory activity when tested on
L. paucicostata 151. After several purification steps, one of the active principles was identified as lutein by MS, UV and NMR spectroscopic analyses. Lutein and its isomer zeaxanthin inhibited benzoic acid-induced flowering in both
L. paucicostata 151 and 381.
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Emiko EZAKI-FURUICHI, Gen-ichiro NONAKA, Itsuo NISHIOKA, Katsuya HAYAS ...
1986 Volume 50 Issue 8 Pages
2061-2067
Published: 1986
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Two new procyanidin trimers (
1,
3) and one tetramer (
2) were isolated in their free phenolic forms from the bark of
Rhaphiolepis umbellata, which is used as the dyeing agent for Oshima Tsumugi. On the basis of
1H- and
13C-NMR spectral examinations and the results of partially acidcatalyzed degradation,
1,
2 and
3 were characterized as epicatechin-(4β→8)-epicatechin-(4β→6)-epicatechin, epicatechin-(4β→8)-epicatechin-(4β→8)-epicatechin-(4β→6)-epicatechin andcatechin(4α→6)-epicatechin-(4β→8)-epicatechin, respectively. In addition, the occurrence of the known compounds,
i.e., (-)-epicatechin (
4), procyanidins B-l (
9), B-2 (
5), B-5 (
6) and C-l (
7), proanthocyanidin A-2 (
10), cinnamtannins A
1 (
8) and A
2 (
14), cinchonains la (
11) and lib (
12), and (-)-catechin 7-
O-β-D-glucopyranoside (
13) in the bark of
Rhaphiolepis umbellata was confirmed.
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Kyozo SUYAMA, Susumu ADACHI, Takahiro TOBA, Taeko SOHMA, Chien-Jung HW ...
1986 Volume 50 Issue 8 Pages
2069-2075
Published: 1986
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The enzymatic transgalactosylation reaction of
O-nitrophenyl-β-D-galactoside and lactose in the presence of sucrose as an acceptor was studied. The main product of
E. coli β-galactosidase was a hitherto undescribed trisaccharide,
O-β-D-galactopyranosyl-(1→6)-
O-α-D-glucopyranosyl-(1→2)-
O-β-D-fructofuranoside, to which we assigned the trivial name isoraffinose. Sucrose was an excellent acceptor to accumulate isoraffinose in good yield. Allolactose and trace of unidentified oligosaccharides were also formed by this reaction.
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Hisao WATANABE, Yoshinori KOBAYASHI
1986 Volume 50 Issue 8 Pages
2077-2082
Published: 1986
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The effects of a high dose of X-rays on the distribution of inorganic elements in living leaves of rice (
Oryza saliva) was studied by X-ray fluorescence element mapping spectrometry (XEMS) and chemical analysis. We found that 10 krad irradiation (1500μmφ) decreased the potassium, magnesium, and sulfur concentrations in the leaves at the irradiated part, and increased the calcium and manganese concentrations. The irradiation exerted its effects on elemental transport up to 4-8 cm distant from the irradiated part along the vein. Measured by XEMS, potassium had higher mobility than those of calcium and manganese. The different mobility between potassium and calcium or manganese may be related to the different chemical status of these elements in the living rice seedlings.
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Hajime HATTA, Naofumi KITABATAKE, Etsushiro DOI
1986 Volume 50 Issue 8 Pages
2083-2089
Published: 1986
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The turbidity and hardness of a heat-induced gel prepared from ovalbumin were examined at various pHs and ionic strengths. Depending on the conditions of the medium, a transparent solution, transparent gel, turbid gel, or turbid suspension was obtained by heating. The hardness was a maximum with the conditions that gave a transparent or slightly turbid gel. The gel and coagulums were solubilized by 1% SDS, but not by 6M urea or 50 mM mercaptoethanol. The solution obtained by SDS treatment contained polymers shorter than octamers.
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Akira HASEGAWA, Koji KIGAWA, Makoto KISO, Ichiro AZUMA
1986 Volume 50 Issue 8 Pages
2091-2094
Published: 1986
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A variety of 1-
O-acyl and 1, 6-di-
O-acyl derivatives of N-acetylmuramoyl-L-alanyl-Disoglutamine methyl esters were synthesized from
N-[2-
O-(2-acetamido-2, 3-dideoxy-4, 6-
O-isopropylidene-D-glucopyranose-3-yl)-D-lactoyl]-L-alanyl-D-isoglutamine methyl ester, and their biological activities were examined in guinea-pigs and mice.
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Akira HASEGAWA, Shuichi HARA, Makoto KISO, Ichiro AZUMA
1986 Volume 50 Issue 8 Pages
2095-2099
Published: 1986
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A variety of the lipophilic derivatives at C-1 and C-6 in
N-[2-
O-(2-acetamido-2, 3-dideoxy-1-thio-β-D-glucopyranose-3-yl)-D-lactoyl]-L-alanyl-(
N1-fatty acyl)-D-isoglutamine methyl esters were synthesized from 2-
N-acetyl-1-S-acetyl-4, 6-
O-isopropylidene-1-thiomuramoyl-L-alanyl-D-isoglutamine methyl ester. Their immunoadjuvant activity in guinea-pigs, and the protective effect in mice infected with
Escherichia coli (E-77156) were examined.
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Kunihiko SAMEJIMA, Yukio OKA, Katsuhiro YAMAMOTO, Ali ASGHAR, Tsutomu ...
1986 Volume 50 Issue 8 Pages
2101-2110
Published: 1986
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Heat-induced gel forming ability of cardiac myosin and reconstituted actomyosin at different weight ratios was investigated in a model system. In this study, myosin alone always had greater binding power than actomyosin under identical conditions of temperature, pH, and salt and protein concentrations. Especially, myosin formed the strongest three-dimensional gel structure with fine filaments and uniform porosity at pH 5.4 and salt concentration <0.2M KCl on heating to 65°C.
We also found that the "cross-linking" role of F-actomyosin, if any, does not seem necessary for enhancing the binding strength, at least of cardiac myosin, since the incorporation of actin in different proportion into the myosin system always produced a weaker gel than that formed by myosin alone at identical protein concentration and thermal processing conditions. However, among acto-myosin complexes, the one with 1:15 weight ratio always yielded a stronger gel than that formed by actomyosin with other ratios of pH 6.0 and 0.6 M KCl. The gel-forming potential of isolated myosin declined with prolonged storage at 4°C. In view of the similarity of the thermogel behavior of cardiac myosin to that of skeletal myosin filaments formed at low salt concentrations, the relationship between the high gelation ability and the native polymer (filament) formability of cardiac myosin is discussed.
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Aoumeur BAALIOUAMER, Brahim Y. MEKLATI
1986 Volume 50 Issue 8 Pages
2111-2114
Published: 1986
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S. S. TOMAR, V. S. SAXENA
1986 Volume 50 Issue 8 Pages
2115-2116
Published: 1986
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Minoru YOSHIDA, Kazunori HASHIMOTO
1986 Volume 50 Issue 8 Pages
2117-2118
Published: 1986
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Minoru YOSHIDA, Kazunori HASHIMOTO
1986 Volume 50 Issue 8 Pages
2119-2120
Published: 1986
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Ken WATANABE
1986 Volume 50 Issue 8 Pages
2121-2122
Published: 1986
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Yasuo KIMURA, Hiroaki FUJIOKA, Hiromitsu NAKAJIMA, Takashi HAMASAKI, M ...
1986 Volume 50 Issue 8 Pages
2123-2125
Published: 1986
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Toshiaki SHINOHARA, Shigeo SUZUKI, Susumu HONDA
1986 Volume 50 Issue 8 Pages
2127-2129
Published: 1986
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Haruhiko TOYOHARA, Yasuo MAKINODAN
1986 Volume 50 Issue 8 Pages
2131-2132
Published: 1986
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Akira HASEGAWA, Eiji SEKI, Koji KIGAWA, Makoto Kiso, Ichiro AZUMA
1986 Volume 50 Issue 8 Pages
2133-2135
Published: 1986
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Akira HASEGAWA, Eiji SEKI, Makoto Kiso, Ichiro AZUMA
1986 Volume 50 Issue 8 Pages
2137-2139
Published: 1986
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Masahito TAYA, Toshiyuki YAGI, Takeshi KOBAYASHI
1986 Volume 50 Issue 8 Pages
2141-2142
Published: 1986
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Ichiro TOMIDA, Ichiro UMEDA, Ikuko KUDO, Hiroshi KAYAHARA
1986 Volume 50 Issue 8 Pages
2143-2145
Published: 1986
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Shintaro KAMIYA, Sachiko ESAKI, Ryuko SANO, Chika YAMAGUCHI
1986 Volume 50 Issue 8 Pages
2147-2149
Published: 1986
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Tsuyoshi NISHITOBA, Hiroji SATO, Sachiko SHIRASU, Sadao SAKAMURA
1986 Volume 50 Issue 8 Pages
2151-2154
Published: 1986
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Using the fruiting bodies obtained from different strains of
Ganoderma lucidum, several terpenoids were analyzed, and the constituents showed a pattern peculiar to the strains. The fruiting body from Strain I contained C
27 lucidenic acids, and that from Strain II contained C
30 ganoderic acids as major products. A sample different from these strains showed a terpenoid pattern similar to that of Strain II. The results indicate that the terpenoid pattern can be useful to distinguish the strains between the two groups.
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Bunsei KAWAKAMI, Toshiaki KUDO, Yoshiko NARAHASHI, Koki HORIKOSHI
1986 Volume 50 Issue 8 Pages
2155-2158
Published: 1986
Released on J-STAGE: April 05, 2006
JOURNAL
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We have established the sequence of a DNA fragment containing the
Escherichia coli N-acetylneuraminate lyase gene (
nanA or
npl). The coding sequence has an open reading frame of 297 amino acids. Amino acid compositions obtained by analysis of amino acids were mostly consistent with those calculated from the DNA sequence. The open reading frame contains a codon sequence of 15 amino acids deduced to be identical to the known N-terminal amino acid sequence of
N-acetylneuraminate lyase. The first methionine was removed from the enzyme because the N-terminal amino acid was alanine.
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Masanobu SUZUKI, Takeyoshi SUGIYAMA, Masashi WATANABE, Kyohei YAMASHIT ...
1986 Volume 50 Issue 8 Pages
2159-2160
Published: 1986
Released on J-STAGE: April 05, 2006
JOURNAL
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Takashi KATO, Koki HORIKOSHI
1986 Volume 50 Issue 8 Pages
2161-2162
Published: 1986
Released on J-STAGE: April 05, 2006
JOURNAL
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Kiyoshi ISONO, Kimie KOBINATA, Hideaki OKAWA, Hiroo KUSAKABE, Masakazu ...
1986 Volume 50 Issue 8 Pages
2163-2165
Published: 1986
Released on J-STAGE: April 05, 2006
JOURNAL
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Kenji YAMAMOTO, Kaoru TAKEGAWA, Hidehiko KUMAGAI, Tatsurokuro TOCHIKUR ...
1986 Volume 50 Issue 8 Pages
2167-2169
Published: 1986
Released on J-STAGE: April 05, 2006
JOURNAL
FREE ACCESS