-
Takashi INAGAKI, Hiroo UEDA
1987 Volume 51 Issue 10 Pages
2635-2639
Published: 1987
Released on J-STAGE: April 05, 2006
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Microbial hydrolysis of the acetates of unsaturated cyclic terpene alcohols by
Pseudomonas sp. NOF-5 isolated from soil was investigated. (±)-
trans-Carveyl acetate ((±)-
trans-
3) was enantioselectively hydrolyzed with NOF-5 strain to give (-)-
trans-carveol ((-)-
trans-
2 of 86.6% optical purity). However, the hydrolysis of (±)-
cis-
3 was less enantioselective, while (±)-piperitylacetate ((±)-
6, a
cis and
trans mixture) was hydrolyzed to give the (-)-
trans- and (-)-
cis-piperitols ((-)-
trans-
5 and (-)-
cis-
5) in a poor optical yield. In this case, other
tert-alcohos, (+)-
trans- and (-)-
cis-2-
p-menthen-1-ols ((±)-
trans-
7 and (-)-
cis-
7), were also produced. Furthermore, microbial and enzymic allyl rearrangements of (+)-
trans-
6 and (-)-
trans-verbenylacetate ((-)-
trans-
11) were studied. Biological treatment of (+)-
trans-
6 and (-)-
trans-
11 with NOF-5 or its esterase gave (+)-
trans- and (-)-
cis-
7 and (+)-
cis-3-pinen-2-ol ((+)-
cis-
12), respectively.
View full abstract
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Masaharu KANAOKA, Chigusa KAWANAKA, Takaharu NEGORO, Yoshikazu FUKITA, ...
1987 Volume 51 Issue 10 Pages
2641-2648
Published: 1987
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The structural gene for the streptococcal acid glycoprotein (SAGP), an antitumor glycoprotein produced by
Streptococcus pyogenes Su, was cloned in
Escherichia coli, and the nucleotide sequence was determined. The deduced amino acid sequence of SAGP was composed of 410 amino acids, and was highly homologous with colicin E1, a bacterial antimicrobial protein. The SAGP gene was subcloned into an expression plasmid and was expressed in
E. coli. The gene product was demonstrated to have almost the same molecular weight and an immuno-chemical property with those of the native SAGP.
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Akinori AMEMURA, Joaquin CABRERA-CRESPO, Masatomo MAEDA, Masamitsu FUT ...
1987 Volume 51 Issue 10 Pages
2649-2656
Published: 1987
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In an attempt to finding polysaccharides with immunostimulating activity, we isolated 14 bacteria that produced polysaccharides from soil. The polysaccharides produced by these bacteria and by strains kept in this laboratory and commercial polysaccharides were tested for ability to activate J774.1 macrophage-like mouse cells. Acidic polysaccharides, especially those from the newly isolated bacteria, induced spreading of the cells and release of prostaglandin E
2 from the cells, which are manifestations of a state of activation. The prostaglandin E
2-releasing activity of the polysaccharides was 1/10 to 1/20 on a weight basis of that of
Escherichia coli lipopolysaccharide, a potent immunostimulant.
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Tetsuo MURO, Yoshio TOMINAGA, Shigetaka OKADA
1987 Volume 51 Issue 10 Pages
2657-2663
Published: 1987
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The protease from
Streptomyces cellulosae preferentially catalyzed the condensation reaction producing tripeptide amides in highly concentrated mixture solutions of various dipeptides and amino acid amides, although it weakly hydrolyzed the substrates at the same time. The tripeptide amides formed were L-Leu-Gly-Gly-NH
2 (P
LGGN) from L-Leu-Gly and Gly-NH2 and L-Leu-Gly-L-Leu-NH
2 (P
LGLN) from L-Leu-Gly and L-Leu-NH
2. Moreover, the ratio of the rate of P
LGLN formation per the proteolytic activity of this enzyme was much larger than those of the other proteases tested.
The formation of P
LGLN was studied at various concentrations of the substrates (L-Leu-Gly and L-Leu-NH
2). The dependences of the initial velocities of P
LGGN, formation on the substrates concentrations could be explained by a two-substrate, one-product reaction mechanism involving a single active center forming the peptide bonds and two substrate-binding sites. The values of the substrate dissociation constants for enzyme-substrate complexes were about 0.6 M for L-Leu-Gly and 0.008M for L-Leu-NH
2.
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Philip O. OWUOR, Calleb O. OTHIENO, Hiroshi HORITA, Tojiro TSUSHIDA, T ...
1987 Volume 51 Issue 10 Pages
2665-2670
Published: 1987
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High rates of nitrogenous fertilizers increased the caffeine content, as well as those compounds imparting inferior and superior flavor contents. However, the flavor index, the ratio of compounds imparting superior flavor to those contributing to inferior flavor characteristics, decreased with increasing rates of nitrogenous fertilizers. Although theaflavins generally increased while thearubigins decreased with nitrogenous fertilizer rates, the relationships were quadratic. On the average, the minimum value of theaflavins was produced at about 235 kg N/ha/year.
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Michiko MORI, Isamu SHIIO
1987 Volume 51 Issue 10 Pages
2671-2678
Published: 1987
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Brevibacterium flavum mutants defective in the phosphoenolpyruvate (PEP)-dependent glucose phosphotransferase system (PTS) were selected with high frequency by 2-deoxyglucose-resistance. Most of them (DOG
r) still had the fructose-PTS and grew not only on fructose but also on glucose like the wild-type strain. A mutant having 1/8th the fructose-PTS activity of the wild strain but normal glucose-PTS activity was isolated as a xylitol-resistant mutant. It grew on glucose but not on fructose. The glucose-PTS was active on glucose, glucosamine, 2-deoxyglucose and mannose, and slightly on methyl-α-glucoside and
N-acetylglucosamine, but not on fructose or xylitol. The fructose-PTS acted on fructose and xylitol, and to some extent on glucose but not on glucosamine or 2-deoxyglucose. Mutants unable to grow on glucose (DOGGlc
-) derived from a DOG
r mutant were all defective in the fructose-PTS. All revertants able to grow on glucose derived from a DOG
rGlc
- mutant had the fructose-PTS. The glucokinase activity was about 2/3rds the glucose activity of the fructose-PTS. All the DOGsp
rGlc
- mutants had normal levels of glucokinase. One of these mutants grew on maltose and sucrose, which were hydrolyzed to glucose. Thus, glucokinase seems to contribute to the phosphorylation of glucose liberated inside the cell. The fructose-PTS was induced by fructose and repressed by glucose. The glucose repression was not observed in a mutant defective in the glucose-PTS.
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Harushi NAKAJIMA, Ikuko OSHIMA, Makoto YASHIRO, Koji YODA, Makari YAMA ...
1987 Volume 51 Issue 10 Pages
2679-2689
Published: 1987
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We isolated a temperature-sensitive mutant which did not exhibit derepression of acid phosphatase and invertase at the restrictive temperature. The mutation was mapped in the
cryl gene, the structural gene for the catalytic subunit of adenylate cyclase. On electron microscopic observation of the mutant cells at the restrictive temperature, it was observed that they accumulated carbohydrate particles in the cytoplasm. Isolated particles had a rosette-like structure of 40 to 60 nm in diameter, and were identified as glycogen. After 2hr incubation at the restrictive temperature, glycogen particles occupied most of the cytoplasmic space and the vacuoles were observed to be fragmented.
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Tetsu ANDO, Mizue KOIKE, Masaaki UCHIYAMA, Hiroshi KUROKO
1987 Volume 51 Issue 10 Pages
2691-2694
Published: 1987
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Each of fifty-one conjugated dienes (alcohols, acetates and aldehydes) with a C
12, C
14 or C
16 straight chain was applied to a rubber cap in a sticky-type trap as a single-component lure. Some diene compounds were mixed with their functional derivatives or structurally related monoenes to make two-component lures. In field trials conducted from 1984 to 1986, the new attraction of male moths of nineteen species was observed. These species were classified into nine families as follow: Gracillariidae, Yponomeutidae, Oecophoridae, Cosmopterigidae, Tortricidae (Olethreutinae and Tortricinae), Pyralidae, Pterophoridae, Drepanidae and Geometridae. This result indicates that the conjugated dienes are one of the main groups of lepidopterous sex pheromones. Some interesting information concerned with the taxonomy of the attracted moths and with the characteristics of the chemical structures of their attractants was obtained.
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Taku KATO, Takayuki ORITANI, Kyohei YAMASHITA
1987 Volume 51 Issue 10 Pages
2695-2699
Published: 1987
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[2-
14CH
Z,
E)-γ-Ionylideneethanol and [2-
14C]-(2
Z, 4
E)-γ-ionylideneacetic acid were converted by
Cercospora cruenta to [2-
14C]-(2
Z, 4
E))-1', 4'-dihydroxy-γ-ionylideneacetic acid and [2-
14C]-(2
Z, 4
E)-4'-hydroxy-γ-ionylideneacetic acid, which are intermediates of ABA biosynthesis in
C. cruenta.
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Sumio KITAHATA, Shigeru EDAGAWA
1987 Volume 51 Issue 10 Pages
2701-2708
Published: 1987
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Acremonium sp. 15 a fungus isolated from soil, produces an extracellular enzyme system degrading cyclic (1→2)-β-D-glucan. This enzyme was found to be a mixture of endo-(1→2)-β-D-glucanase and β-D-glucosidase. The (1→2)-β-D-glucanase was purified to homogeneity shown by disc-electrophoresis after SP-Sephadex column chromatography, Sephadex G-75 gel filtration, and rechromatography on SP-Sephadex. The molecular weight of the enzyme was 3.6 × 10
4 by SDS-polyacrylamide gel electrophoresis. The isoelectric point of the enzyme was pH 9.6. The enzyme was most active at pH 4.0-4.5, and stable up to 40°C in 20mM acetate buffer (pH 5.0) for 2hr of incubation. This enzyme hydrolyzed only (1→2)-β-D-glucan and did not hydrolyze laminaran, curdlan, or CM-cellulose. The hydrolysis products from cyclic (1→2)-β-D-glucan were mainly sophorose.
The β-D-glucosidase was purified about 4000-fold. The rate of hydrolysis of the substrates by this β-D-glucosidase decreased in the following order:
p-nitrophenyl-β-D-glucoside, sophorose, phenyl-β-D-glucoside, laminaribiose, and salicin. This enzyme has strong transfer action even at the low concentration of 0.75 mM substrate.
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Gwi Gun PARK, Isao KUSAKABE, Yoko KÔMATSU, Hideyuki KOBAYASHI, T ...
1987 Volume 51 Issue 10 Pages
2709-2716
Published: 1987
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A β-mannanase was purified from the culture filtrate of
Penicillium purpurogenum No. 618 by 1st and 2nd DEAE-cellulose column chromatographies, and subsequent Ultro-gel chromatography. The final preparation thus obtained showed a single band on polyacrylamide disc-gel and SDS-polyacrylamide gel electrophoresis. The molecular weight and isoelectric point were determined to be 57, 000 and pH 4.1 by SDS-polyacrylamide gel electrophoresis and isoelectric focusing, respectively. The purified mannanase contained the following amino acids: glycine>serme>glutamic acid > alanine > aspartic acid. The mannanase exhibited maximum activity at pH 5 and 70°C, and was stable in the pH range of 4.5 to 8 and at temperatures up to 65°C. The enzyme activity was not affected considerably by either metal compounds or ethylenediaminetetraacetic acid. Copra galactomannan (Gal:Man = 1:14) was finally hydrolyzed to galactose, mannose and β-1, 4-mannobiose through the sequential actions of the purified mannanase and the α-galactosidase purified from the same strain.
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Katsuhiro ADCAWA, Yoshiro KOMATSU
1987 Volume 51 Issue 10 Pages
2717-2720
Published: 1987
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The antimutagenic effects of autoxidized Hnoleic and oleic acids on mutagenesis by UV irradiation were investigated in
Escherichia coli B/r WP2 and WP2s
uwA. When added to an agar medium, these autoxidized acids greatly reduced the number of Trp
+ revertants without significant effects on survival in WP2, but no such effect was observed with WP2s
uvrA. The presence of autoxidized linoleic acid decreased the survival of WP2s
uvrA greatly and CM571
recA somewhat. It thus appears that the autoxidized unsaturated fatty acid has antimutagenic effects on the wild type strain and lethal effects on the genetic repair-deficient strains.
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Hiroshi NISHIHARA, Tetsuo TORAYA
1987 Volume 51 Issue 10 Pages
2721-2726
Published: 1987
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Co-flocculation between cells of beer yeast IFO 2018, a flocculent strain, and non-flocculent strains was investigated by means of a chemical modification method. Treatment with periodate deprived non-flocculent cells, but not flocculent cells, of the ability to co-flocculate. Treatment with mercaptoethanol or photo-irradiation in the presence of methylene blue deprived flocculent cells, but not non-flocculent cells, of the co-flocculating ability. Mercaptoethanol-treated or photoirradiated flocculent cells (beer yeast IFO 2018) co-flocculated with periodate-treated flocculent cells, but periodate-treated cells subsequently subjected to mercaptoethanol treatment or photoirradiation neither flocculated by themselves nor co-flocculated with other cells. Thus, it is likely that both protein and carbohydrate components of the yeast cell surface play important roles in the mutual recognition and intercellular interaction involved in flocculation. It is strongly suggested that the essential carbohydrate which is widely distributed among
Saccharomyces species is the mannan fraction on the cell wall, and that a flocculent yeast strain produces surface protein component(s) which recognize and bind the mannan component of adjacent cells.
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Nobuji NAKATANI, Hiroe KIKUZAKI
1987 Volume 51 Issue 10 Pages
2727-2732
Published: 1987
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A new antioxidative glucoside (
1a) was isolated from the leaves of oregano (
Origanum vulgare L.), one of the herbal spices belonging to the Family Labiatae. The structure of
1a was determined as 4-(3, 4-dihydroxybenzoyloxymethyl)phenyl-β-D-glucopyranoside, on the basis of chemical and spectroscopic evidence. In addition, the structure was confirmed by synthesizing its acetyl derivative (
1b).
View full abstract
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Tsutomu HOSHINO, Tsuyoshi TAKANO, Shigeki HORI, Nagahiro OGASAWARA
1987 Volume 51 Issue 10 Pages
2733-2741
Published: 1987
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The biosynthetic origins of the hydrogen, nitrogen and oxygen atoms in the pyrrolidone ring of violacein were established by an anaylses of the
1H,
13C NMR and MS spectra of its isotopeenriched metabolites. Feeding experiments of [2-
2H] and [3-
2H
2]tryptophans have revealed that the hydrogen in the pyrrolidone ring was derived from the methylene protons at the 3-position of tryptophan. The stereochemical fate of the prochiral hydrogens was determined to be in the retention of the
pro-S hydrogen by these feeding experiments using [3
R-
2H] and [3
S-
2H]tryptophans. The incorporation experiment of [α-
15N]tryptophan demonstrated that the nitrogen atom in the ring originated from the α-amino group of tryptophan. The incorporation experiment of
18O
2 gas verified that all the oxygen atoms of violacein were derived from the molecular oxygen.
View full abstract
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Eiko MATSUMURA, Takashi SHIN, Sawao MURAO, Eiko YAMAMOTO, Tatsu KAWANO
1987 Volume 51 Issue 10 Pages
2743-2750
Published: 1987
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New enzymatic colorimetric reactions of benzoic acid derivatives with 2, 2'-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) in the presence of laccase are described. These laccase-catalyzed colorimetric reactions exhibited the characteristic colors arising from a strong absorption maximum in the visible region. Using these colorimetric reactions, benzoic acids
e.g., 4-hydroxy-, 3-hydroxy-, and 3, 5-diamino-benzoic acids, could be determined in micromolar amounts. These methods will offer broad applicability to those enzymatic assays which are designed to release benzoic acids.
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Tom MORITA, Akihiro SANDA, Yoshio TAKIZAWA, Kazuko OHGI, Masachika IRI ...
1987 Volume 51 Issue 10 Pages
2751-2761
Published: 1987
Released on J-STAGE: April 05, 2006
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To determine the distribution of a kidney acid RNase (RNase K
2) and other RNases, the levels of RNase K
2, RNase A, and seminal RNase (RNase Vs
1) in bovine tissues and body fluids were measured by enzyme immunoassay. The crude extracts of several tissues and body fluids were fractionated by phospho-cellulose column chromatography. The enzymatic activities at pH 7.5 and 6.0 and enzyme contents of each tube were measured by enzyme assay and enzyme immunoassay, respectively. In the pancreas, parotid gland, and heart, most RNase activity was due to a single peak of RNase A, but a small amount of RNase K
2 was always observed. In the kidney, there was about 5 times as much RNase K
2 as RNase A. In the lung, although RNase K
2 and RNase A were the major components, there are another two alkaline RNase peaks. In the spleen and liver, there are four RNases, two acid RNases, one of which is RNase K
2, and two alkaline RNases including RNase A. A new acid RNase (non RNase K
2-acid RNase) from both organs was immunologically the same. In serum, there are at least four RNases. By partial purification of serum RNases by phosphocellulose and heparin-Sepharose column chromatographies, at least 4 RNases, RNase A, RNase K
2 and the other two alkaline RNases, one of which is immunologically indistinguishable from liver alkaline RNase, were confirmed. The other serum alkaline RNase was immunologically related to lung and spleen alkaline RNases. In conclusion, in bovine tissues and body fluids there are at least 7 types of pyrimidine-base-specific RNases: brain RNase, seminal RNase, RNase A, RNase K
2, an acid RNase (RNase BSP
1), an alkaline RNase (RNase BL
4), and another alkaline RNase in serum.
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Keiko ABE, Hiroto KONDO, Soichi ARAI
1987 Volume 51 Issue 10 Pages
2763-2768
Published: 1987
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A proteinaceous substance that inhibited the activity of papain (EC 3.4.22.2) was found in seeds of rice,
Oryza saliva L. japonica. This cysteine proteinase inhibitor (CPI) was purified by a series of purification procedures including CM-Sephadex C-50, Sephadex G-75, and DEAE-Sephadex A-50 chromatography. The CPI was a single polypeptide with a molecular weight of about 12, 000, with an isoelectric point at pH 5.3. The CPI was stable below 100°C and between pH 2.2-9.0. The inhibition of papain by the CPI was non-competitive, with a
Ki value of 2.44 × 10
-8 M. The complete inhibition of papain was reached by an equimolar concentration of the CPI.
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Takashi HASHIMOTO, Yasuyuki YAMADA
1987 Volume 51 Issue 10 Pages
2769-2774
Published: 1987
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Tropane alkaloid formation was studied under various culture conditions in suspension cultures of
Hyoscyamus niger L. High aeration increased cell growth and the contents of hyoscyamine and scopolamine. White's medium inhibited cell growth but increased the hyoscyamine content. The low concentrations of nitrogen and phosphate in White's medium were responsible for such effects. NAA promoted cell growth but inhibited hyoscyamine formation. A hyoscyamine content of 0.05% dry weight (DW) (0.14 mg in 25 ml of culture medium) was achieved in modified LS medium in which the phosphate concentration was decreased to 0.2mM and the auxin omitted.
Effects of precursor feeding on alkaloid formation were also studied with the putative precursors of hyoscyamine.
N-Methylputrescine, tropine, phenylalanine, and tropic acid increased alkaloid formation, but the promotive effect of these precursors varied from one experiment to another.
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Tadao ASAMI, Nobutaka TAKAHASHI, Shigeo YOSHIDA
1987 Volume 51 Issue 10 Pages
2775-2780
Published: 1987
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A series of 3-(1-alkylaminoalkylidene)-6-methyl-2
H-pyran-2, 4(3
H)-diones was newly synthesized, and they were assayed as photosynthetic electron transport (PET) inhibitors because of their structural resemblance to cyanoacrylates and 2-alkylaminoalkylidene-l, 3-cyclohexanedione derivatives, which are potent PET inhibitors. Some of the compounds synthesized here showed very high PET inhibition.
View full abstract
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Shigeomi USHIJIMA, Tadanobu NAKADAI, Kinji UCHIDA
1987 Volume 51 Issue 10 Pages
2781-2786
Published: 1987
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A new breeding process for
Aspergillus sojae involving protoplast fusion was studied to obtain more desirable
koji-molds for soy-sauce production, especially as to their enzyme productivities. A pair of double-marker mutants with characteristic enzyme-productivities, derived from genealogically unrelated
A. sojae cultures, were fused by means of the protoplast fusion technique. The fusants were UV-treated, and stable heterozygous diploids were obtained. Further improvement of their enzyme-productivities was attempted using two approaches. Through the usual mutation of the heterozygous diploids, the activities of protease and glutaminase were simultaneously improved to a certain extent. Through the haploidization of the heterozygous diploids with benomyl or
p-fluorophenylalanine (FPA), some haploids considered phenotypically to be recombinants were segregated, in addition to a large number of haploids that had reverted to the original double marker mutants. On screening these haploid-recombinants, the authors obtained a few more excellent recombinants, as to their enzyme productivities, that is, strains producing as much protease as the hyper-protease producer and producing almost as much glutaminase as the hyperglutaminase producing parent. It has been shown that mutation or especially haploidization of heterozygous diploids produced by cell-fusion could be a very useful technique for the breeding of new industrial
koji-molds.
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Masayoshi SAITO, Harue TAIRA
1987 Volume 51 Issue 10 Pages
2787-2792
Published: 1987
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The effects of pH and NaCl on the denaturation of plasma protein during heat treatment were investigated, as well as the relationship between protein structure and emulsifying properties. When the plasma protein solution (1% w/v) was heated at 80°C, precipitation was accelerated by the presence of NaCl. The measurement of SH groups, surface hydrophobicity and CD spectrum revealed that denaturation occurs easily by heat treatment in the neutral pH region and in the presence of NaCl. The emulsifying activity index (EAI) did not change much after heat treatment at pH 3 irrespective of the presence of NaCl, but it decreased about 60% after heat treatment at pH 7 in the absence of NaCl. Gel nitration patterns indicated that a high molecular weight peak arose upon heat treatment at neutral pH. We concluded that the decrease in EAI was owing to the polymerization of serum albumin and γ-globulin, which are the main components of plasma protein, and disulfide bonds participated in this process.
View full abstract
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Hong-Yon CHO, Katsuyuki TANIZAWA, Hidehiko TANAKA, Kenji SODA
1987 Volume 51 Issue 10 Pages
2793-2800
Published: 1987
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We studied the distribution of aminoacylase, an enzyme catalyzing the hydrolysis of
N-acylamino acids, in thermophilic bacteria, and found
Bacillus thermoglucosidius DSM 2542 to be the best producer of the enzyme. The enzyme, purified 13, 400-fold to homogeneity in an overall yield of 34%, has a molecular weight of about 175, 000, and is composed of four subunits identical in molecular weight (43, 000). The enzyme contains 4 g atoms of zinc per mol of enzyme protei-n. The enzyme catalyzes hydrolysis of various kinds of
N-acyl-L-amino acids with very high molecular activity compared to those of fungal and mammalian enzymes:
Vmax and
Km for
N-acetyl-Lmethionine are 3410 units/mg protein and 7.9 mM, respectively. Great stability at high temperatures and with organic solvents and protein denaturants is a characteristic of the enzyme.
View full abstract
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Jeng-De SU, Toshihiko OSAWA, Shunro KAWAKISHI, Mitsuo NAMKI
1987 Volume 51 Issue 10 Pages
2801-2803
Published: 1987
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-
Noboru KAWAGUCHI, Haruki YAMADA
1987 Volume 51 Issue 10 Pages
2805-2806
Published: 1987
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-
Kunihiko WATANABE, Naoko SATO, Kouichi ASANO, Yoshiro HATANAKA, Jungo ...
1987 Volume 51 Issue 10 Pages
2807-2809
Published: 1987
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-
Shigeru SHIGEOKA, Toshio ONISHI, Norihide KISHI, Koji MAEDA, Hirotomo ...
1987 Volume 51 Issue 10 Pages
2811-2813
Published: 1987
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-
Atsushi SHIRAISHI, Hisao FUJII
1987 Volume 51 Issue 10 Pages
2815-2817
Published: 1987
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-
Hitoshi OIWA, Mutsuo NAGANUMA, Shun-ichi OHNUMA
1987 Volume 51 Issue 10 Pages
2819-2820
Published: 1987
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-
Tsutomu YASUI, Mayumi TAKAHASHI, Jun-Ichiro MORITA
1987 Volume 51 Issue 10 Pages
2821-2823
Published: 1987
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Isao KUSAKABE, Mitsuko ZAMA, Gwi Gun PARK, Keisuke TUBAKI, Kazuo MURAK ...
1987 Volume 51 Issue 10 Pages
2825-2826
Published: 1987
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-
Kousaku MURATA, Akira KIMURA
1987 Volume 51 Issue 10 Pages
2827-2829
Published: 1987
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-
Hiromitsu NAKAJIMA, Kaoru NISHIMURA, Takashi HAMASAKI, Yasuo KIMURA, S ...
1987 Volume 51 Issue 10 Pages
2831-2833
Published: 1987
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-
Hiroyuki UKEDA, Shoko TANAKA, Kiyoshi MATSUMOTO, Yutaka OSAJIMA
1987 Volume 51 Issue 10 Pages
2835-2836
Published: 1987
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-
Hiroyuki HORIUCHI, Koji YANAI, Masamichi TAKAGI, Keiji YANO
1987 Volume 51 Issue 10 Pages
2837-2839
Published: 1987
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-
Shohei SAKUDA, Akira ISOGAI, Shogo MATSUMOTO, Mitsuo OGURA, Kazuo FURI ...
1987 Volume 51 Issue 10 Pages
2841-2842
Published: 1987
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-
Tetsuzo TONO, Shuji FUJITA, Hirotaka KAWASAKI, Zhong-Fu LI
1987 Volume 51 Issue 10 Pages
2843-2844
Published: 1987
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-
Hiroyuki TANABE, Yoshinari KOBAYASHI
1987 Volume 51 Issue 10 Pages
2845-2846
Published: 1987
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-
Akira MURATA, Hidetoshi TASHIRO, Akio OGISU, Fumio KATO, Daiji NAITO, ...
1987 Volume 51 Issue 10 Pages
2847-2849
Published: 1987
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-
Sadaki FUJIMOTO, Susumu ISHIMITSU, Haruo KANAZAWA, Tadashi MIZUTANI, A ...
1987 Volume 51 Issue 10 Pages
2851-2853
Published: 1987
Released on J-STAGE: April 05, 2006
JOURNAL
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Eiji IDO, Toyokazu SAITO, Makoto YAMASAKI
1987 Volume 51 Issue 10 Pages
2855-2856
Published: 1987
Released on J-STAGE: April 05, 2006
JOURNAL
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Akio KOBAYASHI, Shinji YATA, Tomokazu HINO, Kazuyoshi KAWAZU
1987 Volume 51 Issue 10 Pages
2857-2860
Published: 1987
Released on J-STAGE: April 05, 2006
JOURNAL
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A new naphthoquinone was isolated from the culture broth of a
Penicillium species and its structure was determined to be (
S)-2, 5, 7-trihydroxy-3-(5'-hydroxyhexyl)-1, 4-naphthoquinone. An alfalfa callus stimulated with the quinone produced an antifungal compound.
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Shinji TANIMORI, Yoshihiko MITANI, Masahiro CHIKAI, Susumu OHIRA, Mits ...
1987 Volume 51 Issue 10 Pages
2861-2862
Published: 1987
Released on J-STAGE: April 05, 2006
JOURNAL
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Eiko MATSUMURA, Takashi SHIN, Sawao MURAO
1987 Volume 51 Issue 10 Pages
2863-2864
Published: 1987
Released on J-STAGE: April 05, 2006
JOURNAL
FREE ACCESS