1. Glucosulfatase is a simply hydrolysing enzyme. 2. It is almost perfectly adsorbed by kaolin. 3. It can not be adsorbed by BaSO4, aluminium hydroxide gel and charcoal. These adsorbents can be used for the purification of the enzyme. Thus a perfectly clear enzyme solution is obtainable. A solid preparation of very active glucosulfatase was obtained. Its purity (G-Sulf) is 11.6 while the original value was 1.0. 4. The enzyme action is inhibited by: glucose, galactose, MgCO3, inorganic sulphate and phosphate. 5. Glucosulfatase always contains phenosulfatase but they are not one and the same enzyme.
(1) The properties and composition of the nigaki oil (a mixture of the shell and kernel oils) obtained from the whole fruit of “nigaki,” Picrasma quassioides Benn., have been examined. (2) As the chief constituent of the fatty acids of the oil, the occurrence of petroselinic acid, C18H34O2 (Δ6:7 octadecenoic acid), has been ascertained. (3) As the other constituent, palmitic acid has been identified from the saturated acids; lauric acid appears also to be present. Of the unsaturated acids, oleic, linolic, and linolenic acids are likely to be present. (4) The shell and kernel oils have also been examined separately. The former contains an appreciable proportion of saturated acids, while the latter has been found to contain petroselinic acid as the chief constituent.