Bioscience and Microflora
Online ISSN : 1349-8355
Print ISSN : 1342-1441
ISSN-L : 1342-1441
Volume 25, Issue 4
Displaying 1-2 of 2 articles from this issue
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  • Terue Shioiri, Keiko Yahagi, Sachie Nakayama, Takashi Asahara, Norikat ...
    2006 Volume 25 Issue 4 Pages 137-146
    Published: 2006
    Released on J-STAGE: November 16, 2006
    JOURNAL FREE ACCESS
    We evaluated the effects of ingestion of a synbiotic fermented milk beverage containing Lactobacillus casei strain Shirota (LcS) at 3 × 1010 and transgalactosylated oligosaccharides (GOS) at 2.5 g per 80 ml (once a day, 2 weeks) on the defecation frequency in 35 female university students with constipation as well as the defecation frequency, intestinal microflora, and intestinal environment in elderly persons in whom the intestinal microflora and the levels of putrefactive metabolites were abnormal in a placebo-controlled double-blind study. In the female students, the defecation frequency after 1 week of synbiotic fermented milk beverage ingestion was significantly higher than that after 1 week of placebo ingestion or before ingestion. In the elderly persons, the fecal Bifidobacterium and Lactobacillus bacterial counts after 1 and 2 weeks of synbiotic fermented milk beverage ingestion were significantly higher than those after placebo ingestion or before ingestion (p<0.05 and p<0.01, respectively). The fecal lecithinase-positive Clostridium bacterial count after 1 week of synbiotic fermented milk beverage ingestion and the fecal Enterobacteriaceae bacterial counts after 1 and 2 weeks of synbiotic fermented milk beverage ingestion were significantly lower than those after placebo ingestion (p<0.05). LcS at 107 CFU per gram of stool was collected during the ingestion period. The acetic acid levels after 1 and 2 weeks of synbiotic fermented milk beverage ingestion were significantly higher than those after placebo ingestion (p<0.01). The stool pH values after 1 and 2 weeks of synbiotic fermented milk beverage ingestion and the ammonia and phenol levels after 2 weeks of synbiotic fermented milk beverage ingestion were significantly lower than those after placebo ingestion (p<0.05). These results suggest that ingestion of the synbiotic fermented milk beverage containing LcS and GOS improves the stool quality, intestinal microflora, and intestinal environment.
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  • Makoto KIMURA, Kuninaga DANNO, Hisako YASUI
    2006 Volume 25 Issue 4 Pages 147-155
    Published: 2006
    Released on J-STAGE: November 16, 2006
    JOURNAL FREE ACCESS
    We screened lactic acid bacteria which had cellular or humoral immunomodulatory function from 51 strains isolated from Mongolian fermented milk by measuring the inductive activity of IL-12 and INF-γ or IgM, IgG and IgA, respectively. Two strains induced large quantities of IL-12 and INF-γ and had cellular immunomodulatory function, and 3 strains induced large quantities of IgA and had humoral immunomodulatory function. The probiotic properties of these 5 strains were studied by measuring the tolerance to stomach acid, bile and pancreatic fluid and the adherence to human enterocyte-like Caco-2 cells in in vitro tests. Results showed that the N-17 strain (Lactobacillus plantarum), which induced large quantities of IgA, strongly tolerated stomach acid, bile and pancreatic fluid and expressed strong adherence to human enterocyte-like Caco-2 cells. In addition, survival of the N-17 strain during passage through the gastrointestinal tract of mice was examined after 6, 12, 24, 36, 48, 60 and 72 hr of oral administration to mice. Six hr after oral administration, the highest number of viable cells of the strain appeared from feces, and almost all of the administered bacteria were recovered within 48hr. These results suggested that the N-17 strain isolated from Mongolian fermented milk induced large quantities of IgA and showed high survival during passage through the mouse digestive tract. Therefore, this is an excellent probiotic strain which has humoral immunomodulatory function and may be useful as a carrier for the oral vaccine.
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