Influences of viable or sterilized Lactobacillus and cellobiose on the fecal microbiota and fecal metabolites in dogs given boiled chicken head and cow milk were investigated. During the intake of boiled chicken head and cow milk, the number of Enterobacteriaceae (p<0.05) and the fecal pH (p<0.05) increased significantly, and the frequency of occurrence of lecithinase-negative and lecithinase-positive clostridia tended to increase in all test groups. Furthermore, fecal concentrations of sulfide increased significantly (p<0.05) or tended to increase in all test groups. Although the numbers of lactobacilli did not decrease during the intake of boiled chicken head and cow milk in the viable Lactobacillus and cellobiose intake group and sterilized Lactobacillus and cellobiose intake group, they decreased significantly (p<0.05) and bifidobacteria were not detected during the intake of boiled chicken head and cow milk in the skim milk intake group. The fecal concentrations of short chain fatty acids (acetic, butyric and propionic acids) were not changed throughout the test period in any test group. Although clinical symptoms such as fecal hardness in dogs given Lactobacillus and cellobiose were not markedly different from those of the skim milk intake group, there were no dogs which excreted only abnormal (soft, muddy to diarrhea) feces in the viable Lactobacillus administration group on day 21. These findings indicate that administration of Lactobacillus and cellobiose offered more protection against fecal microbiota disorders during the intake of boiled chicken head and cow milk than the administration of skim milk, and there is a possibility of alleviating clinical symptoms by the administration of Lactobacillus and cellobiose.
We encountered a neonate with necrotizing enterocolitis (NEC) caused by Klebsiella pneumoniae. Clinical symptoms and inflammation did not improve despite intravenous administration of antibiotics. Bifidobacterium breve enemas were performed. After beginning enema, clinical symptoms and inflammation improved within 24 hours. Probiotic enema has high potential as a new therapy for NEC.
The growth rates of four Bifidobacterium strains were compared in two different culture media containing either 1-kestose (GF2) or nystose (GF3), the main components of fructooligosaccharides (FOS). Of the four Bifidobacterium strains examined, the growth rates of B. longum ATCC 15707T and B. catenulatum ATCC 27539 in the GF2 medium were higher than those in the GF3 medium, whereas the growth rate of B. pseudocatenulatum ATCC 27919T in the GF2 medium was lower than that in the GF3 medium. The growth rate of B. adolescentis ATCC 15705 was the same in both media.