The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Volume 11, Issue 3
Displaying 1-14 of 14 articles from this issue
  • KOOZOO KAZIRO, KANEJI TSUJI
    1930 Volume 11 Issue 3 Pages 333-343
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • On the Digestive Enzymes of the Silkworm
    OSAMU SHINODA
    1930 Volume 11 Issue 3 Pages 345-367
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    The pH of the intestinal juice of the 5th instar larvae of Bombyx mori after being starved for one day is ca. 9.8.
    Proteases, both from the intestinal juice and from the glycerin extract of the intestinal epithelium, have the same optimal pH of 9.5. With the gliadin only, it is ca. 10. The protease can digest both vegetable and animal proteins and peptone.
    The digestion of glycylglycin occurs only with glycerin extract of the epithelium, and its optimal pH is ca. 8.
    Amylase has its pH optimum at 9.5; maltase, at 6.8; and invertase, at 7.0. The composite optimum of amylase and maltase (the saccharogenesis by the crude intestinal juice) is 8.0. Cellulase and Zytase are absent.
    Disaccharide ferments are, as is the case with dipeptide ferment, localized to an extent in the intestinal epithelium.
    The most interesting and important thing is that the depolymerisation of the food materials (protein, starch) begins within the intestinal lumen by the juice secreted therein and goes on most favourably at the natural pH (9.5) of the juice, whereas the final disintegration of the end products of the above, so to speak, preliminary digestion (dipeptides, disaccharides) occurs rather exclusively within the epithelial cells and at more or less neutral reaction on their way from the lumen to the circulatory system.
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  • MUTSUO HEKI
    1930 Volume 11 Issue 3 Pages 369-390
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    In the present paper, data are presented on the distribution of the total fatty acids, the total cholesterol and lipoid phosphorous in the blood, liver, lung, kidney, spleen, heart- and skeleton muscle of anemic rabbits, and the following conclusions are drawn.
    1. Lipemia was observed in case of the phenyl-hydrazine anemia only in a rabbit where anemia was most highly developed prior to death, whereas in case of the bleeding anemia in almost all the test animals even where anemia was not so advanced as because of the hydrazine poisoning.
    2. In the bleeding anemia, the concentration of the total fatty acids in the whole blood was high in every stage of recovery from anemia. The fact that the fat content in the whole blood was yet high in almost recovered ones, in spite of the normal content in the plasma, indicates that even after the recovery from anemia, blood cells were much charged with fat.
    3. In the bleeding anemia, the content of fat in the spleen was also increased till a certain stage of recovery from anemia. The high content of fat returned t onormal after the disappearance of lipemia in the blood plasma. The size of the spleen was not varied thereby. In the phenyl-hydrazine anemia the size of the spleen enlarged itself greatly and the content of fat was not changed on the contrary.
    4. Lipemia was not necessarily accompanied by increase or decrease of fat in the liver. In the lipemi a of the phenyl-hydrazine anemia and in the earlier stage of the bleeding anemia the content of fat in the liver was increased as a rule, compared with the non-anemic rabbits.
    5. An abnormally high content of fat was observed in lung, kidney, heart and skeleton muscle in cases of partial recovery from bleeding anemia.
    6. No definite results were obtained about the distribution of cholesterol throughout the whole experiment. Only in the severest cases of lipemia, in two kinds of anemia, cholesterol increased remarkably in the heart muscle.
    7. In respect to lipoid P. there was not observed any remarkable varia-tion.
    The author offers many thanks to Dr. Osato for his helpful advice in carrying out this investigation.
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  • TADAO SEKITOO
    1930 Volume 11 Issue 3 Pages 391-406
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    1. Die wiederholte Blutentnahme veranlasst eine leichte Hypocaleämie, welche bald nach der Unterbrechung der Blutentnahme zu dem Normalzustand zurückkehrt.
    2. Eine Probelaparatomie übt keinen merklichen Einfluss auf den Blutkalkgchalt des Kaninchens aus.
    3. Die experimentelle Ableitung der Galle nach aussen aus der Gallenblasenfistel verursacht eine allmählich sich einstellende Hypocaleämie.
    4. Diese Hypocalcämie wird durch die Zufuhr von Gallensäure wieder ausgeblieben.
    5. Der Blutkalkgehalt bei experimentellem Stauungsikterus zeigt anfangs eine allmählich sich steigernde Hypercalcämie und späcer eine allmählich absteigende Hypocalcämie.
    6. Eine teilweise Unterbindung der Gallenblase veranlasst eine vorübergehende Hypocalcämie, die bald wieder den normalen Wert erreicht.
    Aus den Daten geht hervor, dass der Gallensäureverlust aus dem Organismus Hypocalcämie, überschüssige Zufuhr von Gallensäure in den Organismus Hypercalcämie erzeugt, und dass die Gallensäure zu der Regulation des Calciumstoffwechsels in inniger Beziehung steht.
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  • TSUNEO MATSUMORI, MIZUHO OKUDA
    1930 Volume 11 Issue 3 Pages 407-414
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • SHIROZO AKAGI
    1930 Volume 11 Issue 3 Pages 415-421
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    1. Die Durchflusszeiten der verschiedenen Flüssigkeiten wurden beim Ubbelohde-Viskosimeter unter den verschiedenen Treibdrucken zwischen 10 und 70mm. Wassersäule bestimmt.
    2. Mit Hilfe der Methode der kleinsten Quadrate wurde genau festgestellt, dass das Produkt der Durchlaufszeit mit dem Druck konstant ist, unabhängig von dem Druck.
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  • SHIROZO AKAGI
    1930 Volume 11 Issue 3 Pages 423-425
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • JUN-ICHIRO SAGARA
    1930 Volume 11 Issue 3 Pages 427-431
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    1. Der Kaninchenorganismus ist befähigt, die einverleibte Ornithursäure zum Teil zu spalten.
    2. Durch Organbrei- and Durchblutungsversuche konnte festgestellt werden, dass die Leber and die Niere des Kaninchens, wenn auch nur in geringer Menge, die zugesetzte Ornithursaure zu spalten vermag.
    3. Der Organismus des Kaninchens ist nicht imstande, die Synthese der Ornithursäure aus der verfütterten Benzoesäure and dem Ornithin auszuführen.
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  • JUN-ICHIRO SAGARA
    1930 Volume 11 Issue 3 Pages 433-436
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    1. In vollkommenem Einklang mit den früheren Angaben babe ich zuerst festgestellt, dass der im Hühnerei vorhandene freie Zucker während der Bebrütung mit fortschreitender Entwicklung des Embryos bis zum 20ten Bebrütungstag abnimmt.
    2. Die weitere Untersuchung ergab, dass im Hühnerembryo der gebundene Zucker bei der Bebrütung eine stetige Abnahme erfährt.
    3. Bei 7- und 10 tägiger Bebrütung tritt eine Vermehrung des gebundenen Zuckers im Eiweiss deutlich zutage, während dabei im Dotter eine Abnahme erfolgt.
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  • V. Über das Verhalten des Chinaldins im Organismus des Kaninchens und des Huhns
    MASAO TAKAHASHI
    1930 Volume 11 Issue 3 Pages 437-439
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • VI. Über das Verhalten der Nitrozimtsäure im Tierorganismus
    MASAO TAKAHASHI
    1930 Volume 11 Issue 3 Pages 441-444
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • VII. The Effect of a Vitamin C given Parenterally to Guinea Pigs fed on a Vitamin Free diet
    T. NAGAYAMA, T. TAGAYA
    1930 Volume 11 Issue 3 Pages 445-460
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    The above results indicate, that all the guinea pigs fed on oatmeal and water, and those injected with dextrose, show a remarkable decrease in body weight. Namely the rate of decrease in final body weight to that at the beginning, in the former and in the latter was respectively, 42% and 39% on an average. Accordingly the length of life was short, i.e. 17-23 days. In spite of this the appetite was fairly good even until the last moment of life. On the dissection after death, we found such signs of scurvy as loosened teeth and hemorrhage in organ tissue. On the contrary, those which were given the rra dish. juice orally, though they showed a little decrease in body weight (26.5%), on the autopsy findings after slaughter, no symptom of scurvy was found. In the case of another one, the rate of decrease in body weight was 43.6%, and it lived for 27 days. But the death was caused by deglutition of pneumonia, and we could not find any symptom of scurvy. In the case of guinea pips which were given “C Sample” both orally and parenterally, the decrease in body weight was not remarkable, so that the rate of decrease in the final body weight to the initial, on the oral side and on the parenteral side, was but 15.3% and 23.9% respectively. The amounts of food ingested were normal. The animal was entirely healthy under feeding for more than a month, and besides no abnormal condition was recognised at all. On dissection, we could not find even the slightest symptom of scurvy. But in either case, the decrease in body weight was indispensable. As McCollum, Simmonds and Pitz (1917) already stated, this fact rises from oats being not only imperfect in amino acid and salts but also devoid of vitamin A.
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  • VIII. Contribution to the Study on the Carbohydrate Metabolism of Guinea Pigs Fed on a Vitamin C Free Diet
    YOSHIYUKI KOGA
    1930 Volume 11 Issue 3 Pages 461-477
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • MORIO YASUDA
    1930 Volume 11 Issue 3 Pages 479-503
    Published: 1930
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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