The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
37 巻, 4 号
選択された号の論文の10件中1~10を表示しています
  • II. HOMOGENTISICASE
    MASAMI SUDA, YOSHIRO TAKEDA
    1950 年 37 巻 4 号 p. 381-385
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
    1. From rabbit liver we have isolated and purified an enzyme, which catalyzed the oxidative breakdown of homogentisic acid with the rupture of benzene ring.
    2. The enzymatic reaction proceeds in two steps. In the first benzene ring is ruptured accompanying the consumption of 1 atom of oxygen, and both fu maric acid and precursor of acetoacetic acid are produced. In the second the precursor is then converted to acetoacetic acid with uptake of 1 atom of oxygen. In this step of the reaction inorganic phosphate is required. This may suggest that phosphorylation is involved in the reaction.
    3. We designate the enzyme which ruptures the benzene ring as “homogentisicase.”
    4. Homogentisicase consists of a dialysable co-enzyme, apo-enzyme, and ferrous ion.
    5. The relation between ascorbic acid and metabolism of tyrosine was discussed.
  • II. FATTY ACIDS AND THEIR DERIVATIVES
    YOSHIAKI MIURA
    1950 年 37 巻 4 号 p. 387-395
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
  • RYUZO IWATSURU, TARO NAKAMURA
    1950 年 37 巻 4 号 p. 397-408
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
    Our results would be concluded in the followings.
    1. The excreted fats is constant, even if various sorts of fats are added to the fundamental diets (to 150g.)
    2. The excreted fats in feces…………………………cg.
    Ingesting fats in diets…………………………xg.
    a) Coefficient of excreted fats in feces.…………y%
    y=c/x×100
    b) Digestibility of fats in diets…………y%
    y=(1-c/x)×100
    c) Absorbed fat…………yg.
    y=x-c
  • RYUZO IWATSURU, TARO NAKAMURA, TATSUO INOKUCHI
    1950 年 37 巻 4 号 p. 409-420
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
    1) The excretion of fecal N as protein constituent is constant, independent on the amount of the ingesting proteins.
    2) The ingesting proteins may be absorbed in the intestine for 98-100 per cent.
  • TAIJI SHIMIZU
    1950 年 37 巻 4 号 p. 421-433
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
    The new method of the determination of α-ketoglutaric acid in blood and urine is offered which is sufficiently sensitive and specific. It is based upon the difference of the formation rate and solubility of 2, 4-dinitrophenylhydrazones of α-ketonic acids and the ease of decomposition of β-ketonic acids by aminoantipyrine. The final reading was made colorimetrically, adding alkali to the hydrazone of α-ketoglutaric acid. The extract with xylene in this method can be used for the determination of pyruvic acid. Thus it is possible to determine the amount of pyruvic acid and of α-ketoglutaric acid by using the same sample.
    The author wishes to express his sincere appreciation to Professor N. Shimazono for his encouragement and advice during the progress of this work.
  • II. IDENTITY OF GLUCOTRANSFERASE AND β-GLUCOSIDASE
    KATSUO TAKANO, TOMOO MIWA
    1950 年 37 巻 4 号 p. 435-444
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
    1). Enzymatic transfer of glucose from aryl-β-glucoside to alcohol, which had been observed with enzyme preparations from green leaves, was also recognized in usual β-glucosidase preparations, such as apricot emulsin and enzymes of mold fungi, Aspergillus niger and Penicillium chrysogenum.
    2). From the parallelism between transferring and hydrolyzing activity in enzyme preparations of different purity, it is suggested that the enzymes responsible for transfer and hydrolysis are identical.
    3). The hydrolysis and transfer action of β-glucodidase may be interpreted in terms of acceptor difference; when water acts as glucose acceptor the hydrolysis results, whereas the use of alcohol leads to the formation of alkyl-β-glucoside.
    Present research has been carried out with the aid of the Science Research fund of the Ministry of Education.
  • AKIJI FUJITA, KWANJI MATSUURA
    1950 年 37 巻 4 号 p. 445-458
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
    1. The improved lumiflavin method for estimating free and -esterified B2 is described in detail.
    2. The lumiflavin method is exact, reproducible and specific for B2, the recoveries being always practically cemplete and the blank fluorescence can be completely removed by permanganate treatment.
    3. Free and esterified B2 are equally converted to lumiiavin without preliminary enzymatic hydrolysis.
    4. The distribution of free and esterified B2 in animal and vegetable tissues is reported.
  • KATASHI MAKINO, FUMIO ITOH, KIYOHIKO OHTA
    1950 年 37 巻 4 号 p. 459-460
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
  • XII. FORMATION OF KYNURENIC ACID FROM D-ABRINE IN THE ANIMAL BODY
    TAKASHI KOMAKI
    1950 年 37 巻 4 号 p. 461-466
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
    1. A relatively large amount of kynurenic acid together with a small quantity of kynurenine yellow-like substance were obtained in the urine of the rabbits, to which D-abrine was administered.
    2. About 1/3 of administered D-abrine was excreted in the urine unchanged.
    3. The hematopoetic action of D-abrine is very weak.
    4. The course of the formation of kynurenic acid from D-abrine has been discussed.
    We are indebted to the Department of Education for the grant of “Research Fund for Science”, with the aid of which, this work was carried out. (Ryozo Hirohata)
  • 1950 年 37 巻 4 号 p. 466
    発行日: 1950年
    公開日: 2008/11/18
    ジャーナル フリー
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