巻号一覧
38 巻, 2 号
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KAZUMI YAMASAKI1951 年 38 巻 2 号 p. 93-98
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーDie Galle von Hühner, von Citellus mongolicus ramosus, Thomas und von Schafen wurde untersucht und die Ergebnisse sind folgende:
1. Aus der Hühnergalle wurde die höhere Gallensäure C27H46O6 isoliert, die zuerst aus der Galle von _??_Gigi_??_-fisch (Knochenfisch) dargestellt wurde.
2. Das taurocholsaure Natrium machte den Hauptbestandteil der Galle von Citellus mongolicus ramosus, Thomas aus.
3. Aus der Schafgalle wurde ausser den Hauptgallensäuren, Cholsäure und Desoxycholsäure auch eine minimale Menge von Chenodesoxycholsäure isoliert.抄録全体を表示PDF形式でダウンロード (413K) -
I. NORMAL VALUES AND THE EFFECTS OF EXERCISE AND THIMAMINE OR RIBOFLAVIN INJECTIONTAIJI SHIMIZU1951 年 38 巻 2 号 p. 99-108
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. The normal value of pyruvic acid in the human blood was 0.85±0.02 mg./dl., and that of α-ketoglutaric acid was 0.60±0.02 mg./dI. The normal excretion of pyruvic acid in daily human urine was 21-t 1.7mg. and the concentration of the acid of daily urine was 0.91±0.11 mg./dl. The normal excretion of α-ketoglutaric acid in daily urine was 30±3.6 mg. and the concentration of the acid of daily urine was 1.10±10.12 mg./dl.
2. After exercise both blood pyruvate and α-ketoglutarate was increased, and the latter returned to the normal value faster than the former.
3. One hour after the injection of 3mg. of thiamine both pyruvate and α-ketoglutarate in blood were decreased. One hour after the injection of 10 mg. of riboflavin pyruvate in blood increased slightly and α-ketoglutarate decreased. One hour after the injection of both vitamins together, pyruvate did not show any remarkable change, whereas a-ketoglutarate was decreased.
4. The thiamine injection repressed the increase of both acids after exercise, and the riboflavin injection repressed the increase of α-ketoglutaric acid stronger than that of pyruvic acid. When both vitamins were injected together, the repression was the strongest.
The author wishes to express his sincere'thanks to Prof. N. Shimazono for his guidance and helpful advice.抄録全体を表示PDF形式でダウンロード (649K) -
KOYATA NIWA1951 年 38 巻 2 号 p. 109-114
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. From Aspergillus niger, β-glucosidase preparation with very high activity (Fph.-β-g., 1050) was obtained by means of repeated tannin precipitation followed by adsorption with aluminum hydroxide gel B.
2. This preparation contained 15.7 per cent of nitrogen and no ash.
3. The preparation is almost free from oligases such as a-gluco-sidase, maltase, α- and β-galactosidase with a very minute content of saccharase.
The author wishes to express his gratitude to Prof. Tomoo Miwa for his constant advice and helpful criticism. Thanks are also due to Miss Sumiko Ishikawa for her technical assistance throughout this work.抄録全体を表示PDF形式でダウンロード (333K) -
YOSHIO KURODA1951 年 38 巻 2 号 p. 115-118
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーAmino acids of clupeine, scombrine and spheroidine were determined on their acid hydrolysates by the copper method combined with paper partition chromatography.
(A summary of this treatise was given as a lecture at the 22nd General Meeting of the Nippon Seikagakkai (Japanese Biochemical Society) on 27, April 1950. It was 3 March 1951, that I received and found a paper on a similar subject by K. Felix et al in Z. physiol. Chem., 286, 67 (1950).)抄録全体を表示PDF形式でダウンロード (234K) -
MITSUNORI MARUYAMA1951 年 38 巻 2 号 p. 119-133
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. When guinea pig serum was diluted with distilled water and left alone for 30 minutes at 37°, its complement activity disappeared.
2. The midpiece in the water complement could not be fixed with the sensitized corpuscles.
3. The fourth component existed in an inactive form in the water complement.
4. The fourth component which had been freed from the mid-piece was reversibly affected by the action of water and heat.
5. If the fraction containing both the fourth component and mid-piece was treated with water at 37°, the latter turned to a state not to be fixed by sensitized corpuscles.
6. The substance selectively adsorbed by tonearth from the water albumin fraction, inhibited the midpiece to combine with sensitized corpuscles. This substance was converted to the fourth component by treating with sodium chloride. Therefore the substance inactivating the midpiece in the water complement was considered to be the fourth component altered by an action of water reversibly.
7. The midpiece in the globulin fraction separated from the water complement by CO2 treatment was inactive, and, therefore, that midpiece was considered to be combined with the inhibiting substance in this fraction.
These facts may suggest that in the water complement the mid piece is combined with the altered fourth component and the former can not be fixed with sensitized corpuscles.
8. The fourth component was inactivated by digestion with pan-creatin. The fraction containing only the fourth component showed the colour and the precipitation reactions of protein.
9. The fourth component was inactivated by hydrogenation. The double bond seemed, therefore, to be an essential structure of the fourth component.
The author wishes to express my sincerest thanks to Prof. S. Fujimura for his kind asdvices and encouragement in carrying out this investigation.抄録全体を表示PDF形式でダウンロード (908K) -
I. FATE OF L-RHODINIC ACID AND ITS ALLIED ACIDS IN THE ORGANISMRYOZO HIROHATA, SEIICHIRO YAMASAKI, TOSHINORI ISEKI, TOKUWA TO, SYUN I ...1951 年 38 巻 2 号 p. 135-146
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. L-Rhodinic acid, when administered to a dog, cats, rabbits, and human beings, undergoes ω-oxidation to a large extent, and will be excreted in the urine as dihydro-Hildebrandt acid, the amount reaching about 25% of the ingested quantity in the case of rabbits.
2. A relatively large amount of unchanged acid will also be put out in the urine of rabbits and human beings, amounting in rabbits to more than 11% of the applied rhodinic acid.
3. Dihydro-Hildebrandt acid undergoes another oxidation, such as α, β-desaturation, and leaves the organism as Hildebrandt acid.
4. Hildebrandt- and dihydro-Hildebrandt acids were diminished in the urine together with tetrahydro-Hildebrandt acid after the application of tetrahydrogeranic acid to a man. It is a further evidence of α, β-desaturation of fatty acids in the organism.
5. Hildebrandt- and dihydro-Hildebrandt acids were isolated from the urine of a man to whom geranic acid had been administered.
6. Excretion of a large amount of ω-oxidation product of rhodinic acid or allied substance, and of a detectable amount of desaturation product of di- or tetrahydro-Hildebrandt acid may be probably due to the hindrance of β-oxidation by the branched chain at β- or α-carbon, or both.
We are indebted to Prof. Katsura and Nozoe, and also to Takasago Chem. Industry Co. for research materials, and to Prof. Sebe for his valuable assistance in some experiments. This work was carried out partly by the aid of the Scientific Research Funds of the Ministry of Education.抄録全体を表示PDF形式でダウンロード (666K) -
I. ON THE ACTIVATION OF PANCREAS LIPASETOSHIICHI YAMAMOTO1951 年 38 巻 2 号 p. 147-155
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. It is demonstrated that polyvinyl alcohol (I%), lauryl polyvinyl alcohol (2%) and sodium carboxy methyl cellulose (4%) are effective in emulsifying the natural fat about in the same degree as gelatin, and the polyvinyl alcohol is used as the most convenient dispersing agent in the experiments.
2. Dicarboxylic amino acids and diamino acids including ornithine have a notable stimulating effect (about 50%) on the pancreas lipase for the triacetin and olive oil hydrolysis, with the exception of arginine for the triacetin hydrolysis. An accelerating effect of histidine on the lipase for the olive oil hydrolysis is more than 100%.
3. Among several organic acids, succinic and citric acids are also effective (50%) on the lipase action. While fumaric acid remains almost without influence, maleic acid exhibits a notable stimulating effect on the lipase, as succinic acid. This fact suggests a certain significance of cis-form of these compounds.
4. With regard to the significance of the free amino group and the carboxyl of the amino acids, benzoyl-L-aspartic acid, benzoyl-L-glutamic acid and L-asparagine show almost similarly an accelerating influence (50%) as free aspartic, glutamic or succinic acids. Therefore, the free amino groups of these two amino dicarboxylic acids as well as one carboxyl of aspartic acid seem to have little influence onthe lipase.
Effect of lysine appears, on the contrary, to have its relation to both the free amino groups of the acid.
5. The pancreaslipase solution incubated with alkali at pH 9.2 and at 37° for 24 hours, loses 50% of its activity, which is not completely, but to about 85 % protected from alkali inactivation in the presence of aspartic acid. When the acid is afterwards added to the alkali treated enzyme, it is also able to reactivate the loss of lipase activity to about 85%, as asparagine and succinic acid.
The author has been much indebted to Prof. Dr. Senji Utzino for his hearty guidance and helpful suggestions. These investigations owe much to the Ministry of Education for its grants for the Scientific Researches, for which the author wishes here to express his deep appreciation.抄録全体を表示PDF形式でダウンロード (558K) -
II. ON CEREBRON SULPHURIC ACIDTEISHI NAKAYAMA1951 年 38 巻 2 号 p. 157-160
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーThe author isolated cerebron sulphuric acid after the method of Blix from pig brain.
To identify whether or not cerebron sulphuric acid has primary alcohol group, triphenylmethylchloride was made use of. The latter, however, did not react with the former, indicating any group of primary alcohol does not exist in the molecule of cerebron sulphuric acid. The configuration of cerebron sulphuric acid should be, as Blix reported, as follows:
The author wishes to express his deep indebtedness to Prof. M. Yasuda for helpful critisisrn of this work.抄録全体を表示PDF形式でダウンロード (225K) -
KUNIO YAGI1951 年 38 巻 2 号 p. 161-169
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (542K) -
TAKAMITSU SEKINE1951 年 38 巻 2 号 p. 171-179
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. The motion of pig spermatozoa is activated by Ach and depressed by eserine.
2. The average QAch value of spermatozoa in ejaculate is 15.4 and that of epididymal spermatozoa 18.0. These values are comparable with that for ChE of mammalian brains. The number of Ach molecules split by one spermatozoon per second has been calculated from ZAch value of ejaculated spermatozoa to be 1.35×106.
3. ChE in pig seminal plasma is about one third active as in human serum.
4. ChE of spermatozoa does not splits benzoylcholine; it splits butyrylcholine in higher rate than the enzyme in brain does.
5. The enzyme in spermatozoa is inhibited by 62% with 0.0025M caffeine, but not with monoiodoacetate. Merzonine inhibits the serum choline esterase by 70%, but has no effect on the activity of the ChE in spermatozoa or brain.
6. The motion of spermatozoa is stifled completely by quinine, merzonine and monoiodoacetate, but is not affected by caffeine which inhibits ChE in spermatozoa.
These facts indicate that pig spermatozoa has a high activity of choline esterase, which is classified in “specific” type.
The author is most grateful to Prof. K. Kodama and Ass. Prof. H. Yoshikawa for their helpful advice; and Mr. T. Usui for his technical assistance.
This work has been aided by a grant from Ministry of Education and Pharmacological Research Foundation.抄録全体を表示PDF形式でダウンロード (551K) -
ATUHIRO SIBATANI, MICHIO FUKUDA1951 年 38 巻 2 号 p. 181-198
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. The effect of variation in water content of developing agents for paper chromatography on the magnitude of RF values was investigated mainly in the case of amino acids, using various organic solvents completely or partially miscible with water.
2. The RF values of amino acids are increased with the increase of water content in a given solvent system.
3. The effect of the change of water content in a given organic solvent on the RF values is more pronounced than the modification of other factors, such as the change of pH or the addition of some hydro-phobic compounds, at least in- the chromatography of amino acids.
4. The maximal RF range of a given set of compounds does not necessarily lie at the lowest water content that affords a non-streaking separation of substances to be chromatographed.
5. In a few instances the order of magnitude of RF values of some amino acid pairs may be reversed by the change of water content in solvents.
6. The effect of increase of water content in developing solvents does not necessarily be reflected by an increase of RF values. With pyronin as a solute, entirely reversed situations are obtained.
7. The consequences of the results obtained were discussed in relation to practical as well as theoretical phases of paper chromatography, and the importance of controlling water content of chromatographic solvents was emphasized.
Lastly, it is our great pleasure to express our hearty thanks to Miss S. Take d a, Medico Biological Institute, Microphagen Pharmaceutical Co., Tokyo, for many suggestions and advices given to this work.抄録全体を表示PDF形式でダウンロード (1110K) -
I. CONCERNING THE ETHER-INSOLUBLE LIPIDS OF LYOPHILIZED HORSE BLOOD STROMATAMIO YAMAKAWA, SHIZUE SUZUKI1951 年 38 巻 2 号 p. 199-212
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. Two kg. of lyophilized horse blood stroma was prepared and extracted with organic solvents.
2. From the ether-insoluble lipid fraction, palmityl-sphingoniyelin and a glycolipid, which is described under the name of hematoside, were isolated.
3. The cleavage products from both lipids were studied in detail.
ADDENDUM: I . After this paper was submitted, Prof. Dr. E. Klenk had the kindness to send us his detailed papers. According to the report (21), he already noticed that neuraminic acid in paper (19) possessed a methoxyl group. He used, thereafter, the term neuraminic acid, C10H19O9N, for the non-methoxyl substance which corresponds our prehemataminic acid. Whereas he prefer for the methoxyl-containing acid C11H21O9N to C10H19O8N, we take the latter for our hemataminic acid.
2. Though the structural study of hematoside is being continued, it is justified to state that the amino group of prehemataminic acid in the original undegraded lipid is probably substituted by an acyl group, since no amino nitrogen is found in hematoside by Van Slyke's method.抄録全体を表示PDF形式でダウンロード (1048K)
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