巻号一覧
40 巻, 6 号
選択された号の論文の15件中1～15を表示しています
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FUJIO EGAMI, MICHIKO ITAHASHI, RYO SATO, TAKESHI MORI1953 年 40 巻 6 号 p. 527-534
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーA procedure was described for the extraction and purification of a soluble cytochrome from strains of halotolerant bacteria. The ab-sorption spectra of this preparation were measured in the visible and near ultraviolet region. The hemin group of the cytochrome was isolated and some of its properties were investigated. From these results it was suggested that this cytochrome may be a hitherto unknown one. Relations to other kinds of cytochrome were also discussed to a certain extent.抄録全体を表示PDF形式でダウンロード (481K) -
III. INHIBITION OF CHOLINESTERASE BY URETHANERYOITI SHUKUYA1953 年 40 巻 6 号 p. 535-545
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. Urethane inhibits the cholinesterase in two qualitatively different ways as the incubated concentration of the former differs.
2. The inhibition of the enzyme by urethane in concentrations below 0.5M is reversible; it increases with decreasing temperature.
3. When urethane is in concentrations above 0.5M the inactivation becomes irreversible and it proceeds according to the first order reaction. It is of high order reaction in respect to the urethane concentration. Activation energy of the reaction was calculated to be 65 kcal.抄録全体を表示PDF形式でダウンロード (471K) -
I. BACTERIAL HISTAMINE- AND PUTRESCINE-DEHYDROGENASESKAZUO SATAKE, HISATOSHI FUJITA1953 年 40 巻 6 号 p. 547-556
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. Two adaptive bacterial enzymes, histamine- and putrescine-dehydrogenases were obtained in a cell-free extract, from the cells of Achromobacter sp., previously adapted to the corresponding amines.
2. The dehydrogenases were found to be flavoproteins, having flavin-adenine-dinucleotide as their prosthetic group, which was rather resistant against dialysis.
3. The inhibition of both enzymes caused by methylene blue, Atabrine, and quinine seemed to be due to their competition with the prosthetic group for the apo-enzymes.
4. The inhibition by these substances was prevented by the previous presence of riboflavin and its 5-phosphate, though their later addition failed to recover the already inactivated enzymes.
5. These results were disccussed in connection with the facts known about animal and plant amine oxidases.
We wish to express our sincere thanks to Prof. K. Miyaki and Prof. T. Yanagita, for their unfailing interest and for many helpful advice and discussion during the course of this work. We also thank Mr. T. Seki for flavin-adenine-dinucleotide offered. A part of the expenditure of this work was defrayed by the Scientific Research Funds from the Ministry of Education, for which grateful acknowledgement is here made.抄録全体を表示PDF形式でダウンロード (612K) -
KIYOO SATOH1953 年 40 巻 6 号 p. 557-561
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (307K) -
KIYOO SATOH1953 年 40 巻 6 号 p. 563-569
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (452K) -
I. COLORIMETRIC DETERMINATION OF GLUCOSE DEHYDRO-GENASE ACTIVITY BY THE TRIPHENYLTETRAZOLIUM BROMIDE (TTB)MOTOOMI NAKAMURA1953 年 40 巻 6 号 p. 571-578
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. A colorimetric quantitative determination of liver glucose de-hydrogenase activity was attempted by use of 2, 3, 5-triphenyltetrazolium bromide as hydrogen acceptor.
2. Reduction of TTB was carried out anaerobically in Thun-bergtubes.
3. At pH 7.4, the Michaelis constant (Km) of the enzyme was 0.075 or 0.085M according as Michaelis-Menten's or Line-weaver-Burk's method was employed.
4. DPN was necessary as hydrogen carrier between glucose de-hydrogenase and terminal hydrogen acceptors.
5. It may appear that flavoprotein (Straub's) may act as hydro-gen carrier between reduced DPN and TTB which functions as a hydro-gen acceptor.
The author is indebted to Dr. Y. Oshima and Dr. M. Shirakawa, of the Laboratory of Biochemistry, Faculty of Agriculture, and Dr. T. Misao, of the First internal Clinic, Faculty of Medicine, Kyushu University, who suggested the problem and gave many valuable advices.抄録全体を表示PDF形式でダウンロード (409K) -
HISAKO NISHIHARA1953 年 40 巻 6 号 p. 579-587
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (454K) -
HISAKO NISHIHARA1953 年 40 巻 6 号 p. 589-597
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーA group of 49 cationic surface active agents were tested for bacterio-static activity against Mycobacterium tuberculosis H37Rv. Many of the substances tested were completely inhibitory at a dilution of 1:105, and a few were highly inhibitory at 1:106 dilution. Only partial growth, if any, was observed at surface tensions of less than about 40-45 dynes per centimeter, although complete inhibitions were obtained with some of the tested substances at as high a surface tensions as 54-57 dynes per centimeter.
I wish to thank Dr. C. Richard Smith of Barlow Sanatorium, Dr. Milton C. Kloetzel of the University of Southern California, Department of Chemistry, and Dr. Cyrus O. Guss of the Department of Chemistry, Colorado Agriculture and Me-chanical College for their expert guidance and advice.
Thanks are also due to the various companies which submitted their products for this study, and to Mrs. Annie Fry and Miss Annie Pearl Fry for their skillful assistance.抄録全体を表示PDF形式でダウンロード (485K) -
SUZUOKI ZIRÔ, SUZUOKI TUNEKO1953 年 40 巻 6 号 p. 599-609
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. The occurrence of an enzyme in mammalian liver was as-certained which was capable to hydrolyze lower thioesters.
2. Of various tissues of rats, the liver was found to have the most active enzymatic activity, while the other tissues exerted almost no activity except kidney and blood. On the other hand, acetyl thiocholine was hydrolyzable not by livers, but well by brain-homogenates.
3. From rabbit livers, we obtained the active enzyme preparation by ammonium sulphate fractionation. Although it contained also O-esterase activity, it attacked thioesters much more rapidly than O-esters.
4. Comparing with O-esterase activity, we investigated several enzymatic properties of S-ester hydrolyzing activity of the liver enzyme, that is, pH-activity, pS-activity, temperature-activity, heat-stability and the influences of atoxyl and other inhibitors. These properties of S-esterase activity are well accordant with those of O-esterase activity.
5. On the .basis of these results, there seemed not to exist S-ester specific hydrolyzing enzyme, but O-esterase itself hydrolyzes thioester possibly due to its loose substrate-specificity.
6. We could obtain no experimental evidence for phosphorolysis of these S-esters by our liver enzyme.抄録全体を表示PDF形式でダウンロード (643K) -
V. GLYCOLIPIDS OF ERYTHROCYTES STROMA AND GANGLIOSIDETAMIO YAMAKAWA, SHIZUE SUZUKI, TETSUYA HATTORI1953 年 40 巻 6 号 p. 611-620
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. In order to elucidate the question as to ganglioside is a mixture or a single substance, a pure specimen of ganglioside was prepared from lyophilized grey matter of bovine brain.
2. Ganglioside prepared by us was the same substance as that of Klenk and electrophoretically homogenous. Its solution in water flowed readily, while the aqueous solution of blood cell glycolipids were extremely viscous.
3. From the physico-chemical properties of aqueous solution, globoside, hematoside and ganglioside are likely to be highly polymerized substances.
The authors are indebted to Drs. S. Kobayashi, O. Ichikawa and S.Katada of the Government Experimental Station for Animal Hygiene for the permission and courtesy by the usage of analytical centrifuge, to Dr. N. Ui of the Institute for Science and Technology for the performance of diffusion studies.
They also thank to Drs. M. Izawa, H. Uchida and G. Matsumura of the National Institute of Health for helpful advice and suggestion about the physicochemical considerations.
The expences for the work were aided by the Scientific Fund, furnished to Prof. Akiya by the Ministry of Education, for which the authors wish to their gratitude.抄録全体を表示PDF形式でダウンロード (1035K) -
I. QUANITATIVE ESTIMATION OF CARBOXYL-TERMINAL AMINO ACID BY IMPROVED HYDRAZINOLYSIS METHODKO OHNO1953 年 40 巻 6 号 p. 621-627
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. An improved method for characterization and estimation of C-terminal amino acids in proteins, by means of hydrazinolysis and dinitrophenylation, is described.
2. Lysozyme is proved to possess one leucine residue at its C-terminal.
The author wishes to express his gratitude to Professor S. Akabori for his kind guidance and encouragements through this investigation, to Mr. K. Narita who supplied DNFB and DNP-amino acids, and also to Mr. A. Nagata for his much techni-cal assistance.抄録全体を表示PDF形式でダウンロード (412K) -
I. SYNTHESIS OF A LABILE FRECURSOR OF PHENYLALANINE BY NON-PROLLFERATING GELLS OF AN AUXOTROPH OF ESCHERICHIA COLIMASAYUKI KATAGIRI1953 年 40 巻 6 号 p. 629-639
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. There was ascertained by bioautography the accumulation of phenylalanine in the culture medium of a phenylalanineless mutant (M-Ph601) of E. coli.
2. It was further found that the synthesis of an unidentified and labile precursor (Compound X) of phenylalanine occurred in the sus-pension medium by shaking the resting (non-proliferating) cells of M-Ph601 with glucose, K, NH4 (or NO3') and inorganic phosphate, neither of which were dispensable for the synthesis.
3. The effects of the concentration of these supplements, and of pH on the synthesis were investigated. The synthesis did not occurred with shikimic acid and ATP in place of glucose. It was inhibited by CO and CN', but not by penicillin.
4. Compound X had no ability to support the growth of M-Ph601 and was readily convertible by heat or acid-treatment to Compound Y, which seemed to be phenylpyruvic acid and was capable of supporting the growth of M-Ph601.
5. The synthesis of Compound X was accomplished only with the cells grown on a minimal medium conatining phenylalanine and not with the cells grown on peptone-broth-agar media.
6. The medium obtainde after shaking the suspension of M-Ph601 or tryptophan requiring mutants (e. g. M-Tr459) with above-menttioned supplements, also contained a factor capable of supporting the growth of a shikimic acid requiring mutant (M-Sh508) of E. coli.抄録全体を表示PDF形式でダウンロード (648K) -
HISAKO NISHIHARA1953 年 40 巻 6 号 p. 641-647
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリーAmino acid analyses of the tuberculin protein and of defatted M. tuberculosis H37Rv streptomycin sensitive and streptomycin resistant variants were done by the starch and Dowex 50 chromatographic nin-hydrin methods of Moore and Stein.
Glutamic acid, alanine, and leucine were present in relatively high amounts. Tyrosine and phenylalanine were present in relatively low amounts. Among the basic amino acids arginine was present in the largest amount. No significant difference in the amino acid ccmoosi-tions of the sensitive and the resistant strains was noted. Also, no outstanding difference was observed between the tuberculin protein and the bacterial cell protein.
I wish to thank Dr. C. Richard Smith for his indispensible guidance and advice and for the use of the facilities of the Laboratory of Barlow Sanatorium. I also wish to acknowledge my indebtedness to Mr. Cornelius Y. Chiamori and Mr. Elmer Rice of the University of Southern California, School of Medicine, Department of Biochemistry, and to Mrs. Annie Fry and Mrs. Annie Pearl Lockett for their invaluable assistance.抄録全体を表示PDF形式でダウンロード (415K) -
YUKITO OOTA, RYOHEI FUJII, SYOZO OSAWA1953 年 40 巻 6 号 p. 649-661
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1. The growth of each embryonic organ, i. e., plumule, epioctyl, hypocotyl, radicle, and cotyledon of Vigna sesquipedalis was followed daily during the course of the germination stage, by determining increase in dry weight and water-soluble and water-insoluble fractions. The water-soluble fraction of each organ was further analyzed in respect to sugars, polysaccharides, nitrogen, pentose nucleic acid, and proteins. The water-insoluble fraction of hypocotyls was estimated for fats and waxes, pectic substances, proteins, hemicelluloses, and cellulose.
2. Growth curves of sigmoid shape were obtained for each anabolic organ, whereas cotyledons lost their weight in an exponential way. Growth of epicotyl did not proceed practically until the middle of the germination stage, when it was enhanced to a considerable rate (epigeal germination).
3. With plumules, epicotyls, and radicles, a general trend that tissue constituents examined change in parallel to the extent of growth of respective organs was observed. In hypocotyl, however, there oc-curred an abrupt ceasing of protein accumulation in the water-soluble fraction and distinct decrease in amount of pentose nucleic acid (PNA) as well as sugars in the same fraction at about the middle of the germi-nation stage. The amount of cell-wall materials grew unceasingly thoughout the stage; especially that of cellulose continued its ex-ponential increase. 4. Cotyledons were found to have a large amount of PNA storage, which together with other tissue constituents studied diminished rapidly as the germination proceeded.
5. A possible role of PNA played in the protein accumulation has been discussed and the epigeal type of germination in the present material was interpreted in terms of acropetal migration of metabolites, above all that of PNA, along the stem axis.
Grateful acknowledgement is made to Prof. T. Mori of this institute for his con-tinuing interest throughout the course of this study.抄録全体を表示PDF形式でダウンロード (842K) -
I. EXPERIMENTS WITH LIVER SLICESTOKURO SATO, TAEKO SUZUKI, HARUHISA YOSHIKAWA1953 年 40 巻 6 号 p. 663-671
発行日: 1953年
公開日: 2008/11/18
ジャーナル フリー1.The conjugation of sulfate with various phenolic compounds and related substances in the presence of liver slices was studied by means of S35-sulfate.
2. The percentage of sulfate incorporated into phenyl sulfate was greater, the lower the ratio of sulfate to phenol.
3. Anaerobiosis as well as oxidation and phosphorylation inhibitors such as cyanide, fluoride, azide, iodoacetate and dinitrophenol inhibit the synthesis of phenyl sulfate.
4. If cationoid group is introduced into the benzene ring of phenol the extent of sulfate conjugation increases. On the contrary anionoid group or halogen decreases the sulfate conjugation.
5. Indole, benzene, aniline and other aromatic substances can combine with inorganic sulfate probably after they are oxidized to pheno Is.
6. Kidney is the only organ other than liver which promotes the synthesis of phenol sulfate in considerable extent.抄録全体を表示PDF形式でダウンロード (535K)
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