巻号一覧
42 巻, 2 号
選択された号の論文の13件中1～13を表示しています
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IV. EFFECT OF SOME PROTEIN PERTURBATORS UPON THE CATALYTIC FUNCTION OF CATALASEMITSUO SUZUKI, TARO TOMIMURA, HITOSHI MIZUTANI1955 年 42 巻 2 号 p. 99-109
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリーThe present experiment deals with the effects of perturbators such as sodium benzoate or salicylate upon the catalytic activity of catalase and the physical constitution of its protein molecule. The results can be summarized as follows:
1. The catalatic activity of catalase decreased with increasing concentration of perturbators added to the system. Under the presence of a small amount of pyrogallol in the reaction system, the enzyme is active both catalatically and peroxidatically. Under such a condition, catalatic activity is recovered by the addition of benzoate. When pyrogallol was added to the system in concentrations equivalent to H2O2, so that the enzyme can behave prevalently as peroxidase, no recovery of catalatic activity was observable by the addition of perturbators.
2. The peroxidatic activity of catalase was decreased by the addi-tion of perturbator to the system only under the condition in which H-donor was added lower than H2O2, so that catalase can behave simul-taneously as catalase as well as peroxidase. Under the condition in which, however, H-donor was added higher than H2O2, so that only peroxidatic activity prevails, the peroxidatic activity of catalase in-creased by the addition of lower concentration of perturbators.
3. Tie structural modification of catalase molecule by perturbators proceeds stepwise with the increasing concentration of perturbators. In higher concentration range of perturbators, an absorption change of catalase could actually be observed, indicating the occurrence of a more progressed modification of the molecule. In this state, the catalytic activity of catalase was greatly decreased.
4. It was proved that catalase shows an oxidative activity under certain experimental conditions. and Dr. R. Shukuya for their suggestions and kind advices during the course of this work.
The present work was supported by the subsidy of the Scientific Research Fund of the Ministry of Education (K. Kaziro).抄録全体を表示PDF形式でダウンロード (688K) -
TOMOICHI KUSUNOKI, HIDEKO KIMURA1955 年 42 巻 2 号 p. 111-122
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリー1. By the enzymatic hydrolysis of serum albumin, methyl orange binding ability of the digested system decreased with splitting time.
2. As follows, three groups are classified, based on the relation between RD and RN, where RD is the rate of survival of the protein calculated from the decrease in dye binding ability of the digested system and RN is that from the increase in N content of non protein fraction.
1. RD_??_RN, peptic hydrolysis at pH 3.8 or 4.3, papaic hydrolysis at pH 5.5. tryptic hydrolysis at pH 6.4.
2. RD<RN, peptic treatment at pH 5.5 or 6.4, tryptic hydrolysis at pH 6.3.
3. RD>RN, tryptic hydrolysis at pH 8.4 or 9.0.
These results are considered to be due to the difference in splitting mechanisms of these enzymes.
3. Molecular extinction coefficient of bound dyes, intrinsic dis-sociation constants, and isobestic point of the absorption curve for the binding of methyl orange by the hydrolyzate at pH 6.7 were fairly the same as those obtained in the case of native albumin.
4. By the addition of hydrochlorides of basic amino acids of the supernatant of TCAA precipitation of the hydrolyzate to methyl orange solution, any binding reaction was not recognized. From these results, it is concluded that for binding of methyl orange not only free amino groups of the protein but also the proper molecular weight and con-stant and suitable configuration around the binding sites for the inter-action are required.
5. Free arginine content per N of TCAA precipitate of the hydro-lyzate increased more or less than that of native albumin. From the results obtained for these arginine content and dye binding ability per N, Bergmann's idea for the specificity of the hydrolytic enzyme action upon the synthetic peptide is considered to be extendible to the case of tryptic digestion of serum albumin at optimal pH of the enzyme.
6. In the case of peptic or tryptic treatment at neutral pH range when RD was smaller than RN, viscosity of the hydrolyzate solution in-creased especially at the initial stage of the reaction. This result sug-gests that aggregation of remained protein molecules occurs by a kind of denaturing action of these enzymes.
The authors express their thanks to Dr. T. Satofor his kindness during these investigations.
This work was supported by the Grant in Aid for Fundamental Scientific Research from the Ministry of Education to which the authors extend their grateful thanks.抄録全体を表示PDF形式でダウンロード (695K) -
TOMOICHI KUSUNOKI, HIDEKO KIMURA1955 年 42 巻 2 号 p. 123-130
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリー1. The ultraviolet absorption curves were obtained by the use of Beckman's spectrophotometer for native serum albumin and peptic or tryptic digest of the protein. In the case of peptic digestion, the absorption at shorter wavelengths than 275mμ increased from that of native albumin and at longer wavelengths decreased. After tryptic digestion the absorption at shorter wavelengths than 275mμ increased but at longer wavelength identical with that of native albumin. After hydrolysis by pepsin or trypsin absorption maximum shifted to 275mμ and extinctions of the digested systems of various steps were in coincidence at this wavelength.
2. In the case of tryptic digestion, SN- and SU-values were in agree-ment, where SN and SU were the splitting rates of the protein based on increases in non protein nitrogen and in ultraviolet absorption of non protein fraction, respectively.
3. In the case of peptic digestion, SN was larger than Su especially at the initial stage of hydrolysis. This difference in the two splitting rates might be due to the difference in the mode of actions of pepsin and trypsin. And Bergmann's theory for the specificity of these enzymes to synthetic peptide was applied for the interpretation of this pheno-menon.
The authors express their thanks to Dr. T. Satofor his kindness during these in-vestigations.
This work was supported by the Grant in Aid for Fundamental Scientific Research from the Ministry of Education to which the authors extend their grateful thanks.抄録全体を表示PDF形式でダウンロード (429K) -
GENKICHIRO TAKAGAKI1955 年 42 巻 2 号 p. 131-139
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリー1. In brain homogenate, the ammonia formation without specific substrates is accompanied by the great decrease of glutamic acid content. In the first 10 minutes, glutamic acid is decreased considerably, but adenylic compounds are scarcely dearninated and glutamine is rather increased. These facts show that the main source of ammonia formation during the first 10 minutes is glutamic acid.
2. Brain slices does not deaminate adenylic compounds contained, and moreover, added adenylic acid is not changed over into inosinic acid. It is concluded that adenylic deaminase is inactive in slices. The transformation between adenylic acid and inosinic acid is not considered to be an active process in brain.
3. The problem of ammonia formation in brain was discussed from its great physiological significance, with special reference to the glutamic acid metabolism.
The author expresses his appreciation to Prof. G. Kato and Prof. T. Hayashi for their interest and encouragement during the course of this work and to Dr. Y. Tsukada for his many suggestions and criticism.抄録全体を表示PDF形式でダウンロード (543K) -
I. ITS PRESENCE IN THE SUPRARENAL BODYKANAKA MORI1955 年 42 巻 2 号 p. 141-150
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリーIt is established that the both hydrolytic and esterified cholesterol esterase systems exist in the suprarenal bodies of male and female rats. Its nature is as follows;
1. The esterifying activity of the enzyme is stronger than its hydrolytic ractivity.
2. The optimum pH of the enzyme stands between 6.8 and 7.0 (6.9).
3. The enzyme is activated by bile salts.
4. Each activity of both enzyme systems is enhanced in proportion to its concentration and is inhibited in the presence of the products of the reaction in proportion to their concentrations.
The author wishes to express his thanks to Prof. Raijiro Taka hashi for his interest and encouragement. Thanks are also due to Mr. Atsushi Irizawa, Mr. Mr. Takeo Tanaka and Mr. Shigeki Umezane for their able collaborations in the course of these experiments.
And this work has been partly supported by a grant in aid for the Miscellaneous Scientific Research from the Ministry of Education in 1953.抄録全体を表示PDF形式でダウンロード (527K) -
II. ITS PRESENCE IN THE TESTES AND OVARYKANAKA MORI1955 年 42 巻 2 号 p. 151-161
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリーFrom this series of experiments, it may be concluded:
1. Both hydrolytic and esterifying cholesterol esterase systems are established in the testes and the ovary of the rat, though their activity is remarkably weak as compared with adrenal cholesterol esterase. The differences of the enzyme activity are not almost recognized between the testes and the ovary.
2. Esterifying activity of these two enzymes are stronger than their hydrolytic activity.
3. The optimum pH of the enzymes are 6.8 (6.6_??_6.9) in the testes and 6.6 (6.5_??_6.8) in the ovary.
4. These two enzymes are activated by bile salts.
The author wishes to express his thanks to Prof. Raijiro Takahashi for his interest and encouragement. Thanks are ablso due to Mr. Mitsuyoshi Ueno, Mr. Atsushi Irizawa and Mr. Shigeki Umezane for their able collaborations in the course of these experiments. And this work has been partly supported by a grant in aid for the Miscellaneous Scientific Research from the Ministry of Education in 1953.抄録全体を表示PDF形式でダウンロード (481K) -
II. HEAT OF HYDROLYSIS OF METAPHOSPHATE EXTRACTED FROM YEAST CELLSAKIRA YOSHIDA1955 年 42 巻 2 号 p. 163-168
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (323K) -
TETSUZO ARIMA1955 年 42 巻 2 号 p. 169-179
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリーIn view of the important clinical significance of the appearance of free bile acid in bile, a method for differential micro-determination of bile acids by using chromatogram has been devised. The advantages of this new method are summarized as follows:
1. Any minute quantity of free bile acid contained in a small amount of human bile is quantitatively determinable.
2. By Murakami's colorimetric determination by using vanillin phosphoric acid reagents, the quantity of trihydroxycholanic acids and that of total dihydroxycholanic acids are determined separately, one as the free acid and the other as the conjugated acid. As dexosoycholic acid and chenodesoxycholic acid can not be separately determined, they are taken as a whole in determination.
3. The recovery of free bile acid added to human bile is ca. 99 per cent.
In conclusion, the author expresses his deep gratitude to Prof. H. Miyake and Prof. T. Shimizu, president of Okayama University, for their directions throughout this research, and also to Dr. E. Murakami, former lecturer of our Department, and Mr. M. Funatsu, assistant Prof. of Biochemistry of Kyushu University for their kind advices.抄録全体を表示PDF形式でダウンロード (646K) -
YASUSHI WATANABE, KENSUKE SHIMURA1955 年 42 巻 2 号 p. 181-192
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリーThe conversion of L-homoserine to L-threonine was catalyzed by yeast cell suspension in the presence of glucose under aeration. The stable enzyme preparation was prepared from acetone powder of yeast. The properties and requirements of this enzyme system are described.
1. ATP is an indispensable component for the enzyme activity.
2. Mg++ stimulates the reaction remarkably. The optimal concentration is about 5×10-3M. Mg++ may be replaced by Mn++
3. Optimal pH of this reaction ranges at pH 6.5 to 7.0.
4. Only L-isomer of homoserine is utilized for threonine synthesis. D-Homoserine is not effective. The threonine synthesized by this enzyme is found to be only L-threonine, other isomers of threonine are not formed.
5. The reaction is completely inhibited by p-chloromercuribenzoic acid at a concentration of 1×10-3M.
6. The enzyme appears to have wide distribution in micro-organisms but is not present in a rat.抄録全体を表示PDF形式でダウンロード (623K) -
KENZO TORII, TADASHIGE MORIYAMA1955 年 42 巻 2 号 p. 193-199
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリー1. Coexistence of ascorbic acid in the solution of tryptophan, histidine or tyrosine caused apparent lowering of the observed values of amino acids. Workers in these field, should make some allowance for in the experiments of enzymological decomposition of amino acids by using the homogenate of various organs.
2. Kynurenine could be detected, by air-bubbling through the mixture of tryptophan and ascorbic acid.
3. A new spot was confirmed on the paper-chromatogram of the mixture of tryptophan and ascorbic acid.
It is planned to study further the quantitative relation and the mechanism of nucleic splitting.
Particular thanks are due to Prof. K. Ichiharain Osaka University who has kindly given us abrine. The authors wish to thank Prof. N. I to for his guidance.抄録全体を表示PDF形式でダウンロード (290K) -
KENZO TORII, TADASHIGE MORIYAMA1955 年 42 巻 2 号 p. 201-206
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリー1. Within the range of our experiments, the optimum pH was at kynurenine formation by air-bubbling through the mixture of tryptophan and ascorbid acid.
2. Cu++ ion was one of the catalyst in this reaction.
3. Inhibition of colloidal platinum in this reaction was about 70 per cent.
4. Action of KCN on colloidal platinum could not be recognized.抄録全体を表示PDF形式でダウンロード (264K) -
VIII. ÜBER DIE REDUKTIONSPRODUKTE VON VERSCHIEDENEN GALLENSÄUREN UND STEROGALLENSÄUREN MITTELST NATRIUMÄTHYLATKENZI MATUMOTO1955 年 42 巻 2 号 p. 207-215
発行日: 1955年
公開日: 2008/11/18
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YASUHARU SEKIZAWA1955 年 42 巻 2 号 p. 217-219
発行日: 1955年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (159K)
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