巻号一覧
43 巻, 5 号
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TAKASHI AKAZAWA, IKUZO URITANI1956 年 43 巻 5 号 p. 579-587
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Changes in the metabolism of sweet potato tissue induced by infection with Ceratostomella fimbriata were studied.
2. The respiration of sound tissue adjacent to the infected tissue was about twice greater as much as the control 72 hours after the fungus inoculation ; thereafter it dropped. Rate of the respiratory increase due to DNP addition was reciprocal to that of the respiratory increase caused by the fungus infection, and the finding was assumed to be caused by the acceleration of ADP generation in the tissue.
3. In the sound tissue next to the infected, inorganic phosphate decreased in line with organic phosphate formation, and a concomitant increase of acid insoluble nitorogen conpounds (protein) occurred, a decrease of acid soluble nitrogen compounds (amino acids and amides) being also observed. These facts indicate an activation of anabolism in the infected sweet potato, but occasionally at one stage (72 hours) Pi increase was found, and the partial participation of an uncoupling reaction or the activation of ATP-ase is suggested.
4. In the infected sweet potato, the levels of phospholipide and RNA remained unchanged.
We wish to express our thanks to profs. Y. Sumiki and S. Funahashi, University of Tokyo, for their cordial advices and helpful suggestions and also for the assistance of Mrs. M. Uritani, Miss Y. Hirata and Mr. M. Egawa.
This communication was already presented before the meeting of the Agriculture) Chemical Society of Japan (Tokyo, December 18, 1954).抄録全体を表示PDF形式でダウンロード (518K) -
TAKASHI AKAZAWA1956 年 43 巻 5 号 p. 589-595
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Metabolic changes of white potato infected with C. f. to which it is strongly resistant were studied by methods similar to those used in a previous study of sweet potato.
2. In sound tissue next to infected tissue of white potato respiratory increase, Pi decrease and concomitant increase in Po were observed. The degree of change in these factors was smaller than that observed in the sweet potato. But the increase in Ninsol. (protein) compensating the decrease in Nsol. (amino acids and amides) reached the same level as in infected sweet potato. Thus the respiratory increase observed in white potato infected with C. f. is thought to be caused by the APT-utilizing reaction.
The author wishes to express his gratitude to Dr. I. Uritani, Nagoya University, for his sincere advice, and to Dr. L. Davis, University of Calif. at Los-Angels, for her gift of Ceratostomella fimbriata from Syngonium auritum.
This communication was presented before the meeting of the Biochemical Society of Japan (Kyoto, April 4, 1955).抄録全体を表示PDF形式でダウンロード (300K) -
TAKASHI AKAZAWA1956 年 43 巻 5 号 p. 597-602
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. To elucidate the nature of the anabolic processes of infected sweet potato, protein synthesis, and the activation of respiratory oxidation and oxidative phosphorylation were determined.
2. In the infected sweet potato, protein increased in every cellular fraction (microsomes, mitochondria, and supernatant). RNA was higher in the microsomal fraction in parallel with the decrease in supernatant. This protein synthesis may be carried out in the microsomes, which are rich in RNA.
3. Respiratory oxidation and the oxidative 'phosphorylation were higher in the mitochondira of infected sweet potato than in those of the control. The activity of respiratory oxidation per unit mitochondrial nitrogen was also higher than in the control.
4. ATP-ase and phosphatase activities were enhanced in the infected sweet potato and the physiological implication was discussed.
The author wishes to express his gratitude to Dr. I. Uritani, Nagoya University, for his sincere advice.
This communication was presented before the Meeting of the Agricultural Chemical Society of Japan (Tokyo, April 1, 1955).抄録全体を表示PDF形式でダウンロード (365K) -
KATASHI ICHIHARA, SHIGENOBU ITAGAKI, YOSHITOSHI SUZUKI, MAKIO UCHIDA, ...1956 年 43 巻 5 号 p. 603-609
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Urocanic acid is decomposed by enzyme, prepared from the rabbit liver, under O2-uptake. This fact became clear by the addition of EDTA to the enzyme solution.
2. When the liver extract was treated with Norit, the partially purified enzyme solution decomposed urocanic acid oxidatively without EDTA.
3. The liver extract of rabbit, administered 0.5 mg. aminopterine daily by injection for 40 days, decomposed urocanic acid oxidatively without EDTA.抄録全体を表示PDF形式でダウンロード (357K) -
SHOZO OTA, TSUTOMU WACHI, MAKIO UCHIDA, YUKIYA SAKAMOTO, KATASHI ICHIH ...1956 年 43 巻 5 号 p. 611-618
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. When the acetone-dried powder of Pseudomonas aeruginosa was kept for a long time in the refrigerator, the pathway from urocanic acid to glutamic acid was lost and the oxidative pathway appeared. In the latter, one atom of oxygen was consumed and by the addition of EDTA one further atom was taken up.
2. Enzyme for the oxidative decomposition of urocanic acid was partially purified from Pseudomonas. The purified enzyme did not form glutamic acid but decomposed urocanic acid under uptake of one atom of oxygen either with or without addition of EDTA. This indicated, that the substance sensitive to EDTA, was removed by the procedure of enzyme preparation.
3. Oxine and α, α'-dipyridyl were proven to be as effective as EDTA but Ag+ and Hg_??_inhibited the decomposition of urocanic acid completely. Hydroxylamine was an exception and 54 per cent of the urocanic acid was decomposed by it without O2-uptake.
4. Among the metal ions, only Cu_??_inhibited the effect of EDTA.抄録全体を表示PDF形式でダウンロード (420K) -
KATASHI ICHIHARA, YUKIYA SAKAMOTO1956 年 43 巻 5 号 p. 619-621
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (149K) -
RYO SATO, SHIGEKI TAKEMORI, MITUO EBATA1956 年 43 巻 5 号 p. 623-633
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. A Bacillus species, strain W-2, possesses a glucokinase as a “constitutive” enzyme, which can be extracted from cells by grinding with glass powder.
2. Cell-free preparations prepared and partially purified from the organism phosphorylate glucose with the aid of ATP, but are completely inactive towards fructose. Mannose, galactose and L-arabinose are also phosphorylated by the preparations at rates of 10, 8 and 15 per cent, respectively, of that for glucose, whereas D-glucosamine, D-ribose, D-xylose and sedoheptulose are all inactive.
3. The pH-activity curve of this enzyme has two maxima at pH 6.9 and 8.4.
4. The activity of this enzyme depends on the presence of either Mg_??_ or Mn_??_. Mg_??_is twice more effective as activator than Mn_??_ as compared at their respective optimal concentrations.
5. The enzyme is strongly inhibited by Cu_??_, Hg_??_, iodoactate and p-chloromercuribenzoate. Alloxan is also inhibitory to the enzyme. The inhibition caused by alloxan can be reversed by cysteine.抄録全体を表示PDF形式でダウンロード (533K) -
KUNIO YAGI1956 年 43 巻 5 号 p. 635-644
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーTo estimate flavin compounds in animal tissues by lumiflavin fluorescence method, the conditions of photodecomposition of flavins to lurniflavin were examined in detail using dilute flavin solutions. The best condition for photodecomposition was 30-60 minutes irradiation by fluorescent lamp at 10-30° of solution temperature and solution pH higher than 13. Under these conditions, the amount of lumiflavin produced exactly showed the original flavin quantity.
A standard procedure is as follows: Warm-water extract of tissue is mixed with equal volume of N NaOH, irradiated under the above conditions, extracted once with chloroform and intensity of fluorescence of chloroform layer is estimated. At the same time, the addition test is made by the same way, and amount of flavin is calculated.
By this procedure, flavin quantities of several samples will be estimated simply within 2 hours.
The author wishes to thank Mr. Jun Okuda for his valuable technical assistance in the coures of this work.抄録全体を表示PDF形式でダウンロード (474K) -
IKURO AKAEDA1956 年 43 巻 5 号 p. 645-647
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Rabbit testis appears to contain C-1-glucokinase which is inhibited in diabetic state.
2. Fructokinase of rabbit testis which phosphorylates carbon 6 is not affected in diabetes.
The author is indebted to Dr. S. Mizuhara for his generous advice. This investigation was supported by the Grant for Scientific Research of the Department of Education.抄録全体を表示PDF形式でダウンロード (169K) -
IKURO AKAEDA1956 年 43 巻 5 号 p. 649-652
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーGlucose, ATP, Mg_??_, and NaF were mixed with the homogenate of rabbit testis, and the barium soluble-alcohol insoluble fraction of the mixture was examined by paper chromatography. A large spot of G-1-P was detected only after incubation at 37° for twenty minutes. Without incubation, no spot of G-1-P was observed. So it seems quite probable that rabbit testis contains C-1-glucokinase.
The author is indebted to Dr. S. Mizuhara for his continued interest and to Dr. T. Baba for his technical advice.
This investigation was supported by a scientific research grant from the Department of Education.抄録全体を表示PDF形式でダウンロード (185K) -
KIKUO OGATAM, MASANA OGATA, YOSHIO MOCHIZUKI, TADAMOTO NISHIYAMA1956 年 43 巻 5 号 p. 653-668
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーFollowing the injection of crystalline ovalbumin into the foot-pads of rabbits, the popliteal lymph nodes were removed and the incorporation of 1-C14-glycine into antibody and other cellular protein fractions was studied, using cell suspensions or cell free homogenates.
The following results were obtained.
1. Under suitable aerobic conditions the cell suspensions of the lymph nodes from immunized rabbits incorporated very rapidly C14-glycine into antibody. The rates of incorporation of glycine into antibody, soluble protein, the protein moiety of ribonucleoprotein and that of desoxyribonucleoprotein, expressed as μM glycine per g. protein per hour, were 11.9, 3.5, 2.1, and 0.9, respectively, when glycine concentration was 0.68mM.
2. The rate of the C14 glycine uptake into antibody was higher than the proteins of the cellular components, fractionated by the procedure of Schneider, of which that of the microsome fraction showed the highest value, that of the soluble fraction the next, and protein moiety of desoxyribonucleoprotein the lowest value.
3. Aresenate, azide, dinitrophenol, monoiodoacetate and anaerobiosis inhibited the incorporation of glycine into antibody as well as into other cellular proteins. Under anaerobic conditions, the addition of glucose, ATP, and other P-compounds restored only slightly the C14-glycine uptake.
4. When the cell free homogenate was used, the rate of the incorporation was greatly depressed. But even in this condition, the isotope uptake into antibody was more rapid than that into other protein fractions. The arsenate, monoiodoacetate, 2.4-dinitrophenol, or malonate inhibited the amino acid uptake in the cell free homogenate system.
This work was supported by a Grant in Aid for Scientific Research from the Department of Education.
The authors express sincerely their gratitude to Prof. Masuo Ogata and Issei Otawara for their advice and encouragement during this work.抄録全体を表示PDF形式でダウンロード (848K) -
II. EFFECTS OF RIBOFLAVIN-MONOSULFATE ON BACTERIAL GROWTHFUJIO EGAMI, MARIKO NAOI, MITSUHIKO TADA, KUNIO YAGI1956 年 43 巻 5 号 p. 669-674
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Riboflavin-monosulfate (FMS) does not support the growth of L. casei.
2. It competes with riboflavin, FMN, and FAD, and when added to growth promoting concentration of these flavins, it inhibits the growth of L. casei.
3. Inhibition indices of FMS are 5, 6, and 63 for FAD, FMN, and riboflavin, respectively. The higher inhibition index found for riboflavin may be explained by the higher permeability of riboflavin.
4. The growth inhibition by FMS is a bacteriostatic action.
5. FMS has no effect on the growth of Str. facalis.
We acknowledge the gift of bacterial strains by the Institute for Infectious Dis-eases and the Institute of Applied Microbiology of the University of Tokyo.抄録全体を表示PDF形式でダウンロード (236K) -
SEIICHIRO NAKATSU1956 年 43 巻 5 号 p. 675-681
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーAn enzyme from hog kidney was found to catalyze the transfer of the amidine moiety of canavanine to glycine with the formation of glycocyamine. The enzyme was sensitive to heavy metal ions, and was inhibited by p-chloromercuribenzoate. No cofactor requirement was observed, and the optimal pH was about 7.4. It was suggested that an isothiourea-type enzyme-arnidine intermediate complex might be formed.
The author wishes to thank Dr. S. Sbibuy a and Dr. N. Izumiya for their valuable advice.抄録全体を表示PDF形式でダウンロード (386K) -
SHIGERU YAMAWAKI1956 年 43 巻 5 号 p. 683-689
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーDiphenyl pyrophosphate is hydrolysed by diaryl pyrophosphatase into two moles of monophenyl phosphate. This enzyme is inactive to flavin adenine dinucleotide, which is in turn split by nucleotide pyro-phosphatase to produce flavin monophosphate and adenylic acid. For either diaryl pyrophosphatase or nucleotide pyrophosphatase there are two isodynamic, acid and alkaline, enzymes. The acid FAD pyro-phosphatase is found in potato extract and the alkaline enzyme in muscle autolysate. Acid diaryl pyrophosphatase contained in potato extract can be obtained free from isodynamic alkaline enzyme, whereas the two isodynamic diaryl pyrophosphatases of the liver, though they could be made free from other phosphatases, are incapable at present, of being separated from each other.
This work was supported in part by a grant from the Ministry of Education, given to Prof. S. Akamatsu, the director of this department.抄録全体を表示PDF形式でダウンロード (489K) -
SAKARU SUZUKI1956 年 43 巻 5 号 p. 691-696
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. The uptake of S35-sulfate into the charoninsulfuric acid of the mucous gland of Charonia lampas has been studied by in vivo and in vitro experiments.
2. An increase in the radioactivity of charoninsulfuric acid was demonstrated during 69 hours after intramuscular injection of S35-sulfate.
3. Sodium sulfate labeled with S35 has been found to be taken up by the slice of the mucous gland and built into charoninsulfuric acid. In the boiled mucous gland no similar uptake occurs.
The author thanks Prof. Fujio Egami for his interest and encouragement. A part of the experiments was executed in the Marine Biological Laboratory of Nagoya University.抄録全体を表示PDF形式でダウンロード (324K) -
SAKARU SUZUKI, KEN-ICHI OGI1956 年 43 巻 5 号 p. 697-700
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーThe incorporation of S35-sulfate into the mucous gland of Charonia lampas was studied by means of autoradiography.
A particularly high uptake of S35 is noted in a layer bordering with the connective tissue in mucous cells. It is shown with Feulgen's nucleal reaction that numerous granules regarded as nuclei are distributed in the layer.
Sections of the liver, obtained 3, 45 and 69 hours after the injection of S35-sulfate, give no blackening of the films in contrast with mammalian livers.
We thank Prof. Fujio Egami for his interest and encouragement. A part of the experiments was executed in the Marine Biological Laboratory of Nagoya University.抄録全体を表示PDF形式でダウンロード (1373K) -
YOSOJI ITO, SUSUMU TSURUFUJI, YOSIO HIRAMATSU, MIKITO CHIBA1956 年 43 巻 5 号 p. 701-710
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Within 4.5 hours' experimental period, rectilinear relationship is observed between time and overall uptake of calcium including physico-chemical exhange and physiological accretion by the permanently growing lower incisor of the rat.
2. No difference is observed in overall uptake of calcium by the incisor between day and night.
3. In the initial period after the administration of radioactive calcium, physicochemical exchange and physiological deposition form, respectively, 40 and 60 per cent of the overall incorporation of Ca45 into the lower incisor of the rat.
4. Correlation between overall uptake of calcium by the incisor and body weight of the animals are discussed.抄録全体を表示PDF形式でダウンロード (575K) -
V. ON A PRINCIPAL INTERMEDIATE IN THE BREAKDOWN OF CHOLIC ACID BY STREPTOMYCESYASUO SABURI, SHOHEI HAYAKAWA, TAKAO FUJII, IKURO AKAEDA1956 年 43 巻 5 号 p. 711-721
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. The culture conditions for the formation of a new unsaturated C-22 acid (presumably 7α-hydroxy-3, 12-diketo-Δ4-bisnorcholenic acid) from cholic acid by S. gelaticus 1164 were investigated by shake bottle culture.
2. All of the seven Streptomyces spp. which were identified in the previous paper (6) degraded cholic acid through an intermediate containing 7α-hydroxy-3-keto-Δ4-ene grouping. This fact was confirmed through the spectrophotometric analyses of the cultures.
3. Ability of the above seven Streptomyces spp. to utilize the various kinds of bile acids was investigated and it was found that the bile acids containing three functional groups at C3, C7 and C12 were utilized except norcholic and homocholic acids. This fact suggests that the utilization of bile acids by Streptomyces is dependent on both the nucleus constitution and the length of side chains of bile acid.
4. An oxidative pathway of cholic acid by Streptomyces was discussed.
The authors express their hearty thanks to Prof. T. Shimizu and Prof. S. Mizuhara for their kind guidances.抄録全体を表示PDF形式でダウンロード (625K) -
VI. ON THE STRUCTURE OF THE UNSATURATED C-22 ACID TRANSFORMED FROM CHOLIC ACID BY S. GELATICUS 1164SHOHEI HAYAKAWA, YASUO SABURI, TAKAO FUJII, YOSHIYA SONODA1956 年 43 巻 5 号 p. 723-730
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーS. gelaticus 1164 converts cholic acid to a new unsaturated C-22 acid with m. p. 280-282° (decomp.) which was previously postulated as 7α-hydroxy-3, 12-diketo-Δ4, 9(11)sali-bisnorcholadienic acid. Further in-vestigations demonstrated that the new acid may be 7α-hydroxy-3, 12-diketo-Δ4-bisnorcholenic acid.
The authors express their hearty thanks to Prof. T. Shimizu and Prof. S. Mizuhara for their kind guidances, and to Mr. Y. Matsui of Research Laboratory, Shionogi & Co., Ltd. for his help in measuring the infrared absorption spectra and for his valuable criticism.抄録全体を表示PDF形式でダウンロード (518K) -
VII. PARTIAL SYNTHESIS OF 3, 12-DIKETO.Δ4, 6-CHOLADIENIC ACID AND ITS DERIVATIVES FROM CHOLIC ACIDSHOHEI HAYAKAWA, YASUO SABURI, TAKAO FUJII, YOSHIYA SONODA1956 年 43 巻 5 号 p. 731-736
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. The identification of the dehydrobromination product of 3-keto-4_??_-bromo-7α, 12α-diacetoxycholanic acid with pyridine was attempted and it was shown that the product having the dienone grouping is 3-keto-12α-acetoxy-Δ4, 6-choladienic acid and it is not in the structure of Δ4, 7-3-ketone reported by Matsumoto (2).
2. The partial syntheses of 3, 12-diketo-Δ4, 6-choladienie acid and its derivatives from cholic acid were described, and these compounds were used to confirm the structure of the various intermediates of cholic acid obtained through the action of S. gelaticus 1164.
The authors express their hearty thanks to Prof. T. Shimizu and Prof. S. Mizuhara for their interests on this study and their encouragements, and to Mr. Y. Matsui of Research Laboratory, Shionogi & Co., Ltd. for his help in measuring the infrared absorption spectra and for his valuable criticism.抄録全体を表示PDF形式でダウンロード (355K) -
Koiti TITSNI, Hiroshi YOSHIKAWA, Kazuo SATAKE1956 年 43 巻 5 号 p. 737-738
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (124K)
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