巻号一覧
43 巻, 6 号
選択された号の論文の16件中1～16を表示しています
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I. AMINO ACID COMPOSITIONS OF CRYSTALLINE BACTERIAL AMYLASE FROM B. SUBTILIS NSHIRO AKABORI, YOSHIMI OKADA, SADAO FUJIWARA, KIN-ICHI SUGAE1956 年 43 巻 6 号 p. 741-748
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーThe amino acid compositions of crystalline bacterial amylase of B. subtilis N has been determined by chromatographic analyses.
The authors wish to express their sincere thanks to Mr. Bunji Hagihara for his kind supply of bacterial amylase.抄録全体を表示PDF形式でダウンロード (349K) -
YOICHI KONDO, TADANORI KAMEYAMA, NOBUO TAMIYA1956 年 43 巻 6 号 p. 749-760
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. β-Mercaptopyruvic acid is decomposed by an enzyme prepared from E. coll.
2. Products of this reaction are pyruvic acid and elemental sulfur. But in the case of reaction with intact cell β-mercaptopyruvic acid is further decomposed to carbon dioxide and hydrogen sulfide.
3. This enzyme is stable against heat in crude state. However, in pure state this thermal stability is lost.
4. The probability of this compound to be in the metabolic path of cysteine is discussed.抄録全体を表示PDF形式でダウンロード (615K) -
IX. PERTURBATION OF CYTOCHROME CKEIZOO TSUSHIMA, TOSHINA MIYAJIMA1956 年 43 巻 6 号 p. 761-777
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーThe modification of cytochrome c by the effect of benzoate, salicylate and lauryl sulfate was studied by measuring the spectral changes, CO-binding affinity and oxidatic activity as the criteria of the structural modification of its protein portion. The result obtained are as follows:
1. When benzoate was added to the solution of reduced cytochrome c, the latter became accessible to the bonding with carbon monoxide, which could be proved by its absorption spectra after the saturation of the solution with CO-gas. It was assumed thus, that sodium benzoate effects for the modification of the protein portion of cytochrome c. This stage of the modification was proved to be reversible.
The relation between the percentages of the formation of modified cytochrome c delivering CO-compound and the concentration of benzoate presented was indicated by a sigmoid curve of a high order reaction.
2. A similar result was obtained by using sodium salicylate in place of sodium benzoate. In this case, however, the modification of cytohrome c occured in lower concentration than in the case with benzoate. At 0.8M sodium salicylate, the absorption of the CO-compound of the modified cytochrome c reached the final figure.
Further increase of the salicylate concentration caused a diversed changes in the spectrum of reduced cytochrome c even without the presence of carbon monoxide. This absorption change could distinctly be observed also by the addition of lauryl sulfate. These absorption changes were proved to be irreversible. More serious modification seemed to be occuring, in this case, in the protein portion of cytochrome c.
3. Investigating the oxidase activity of modified cytochrome c with ascorbic acid as the substrate, the relation between the percentages of the promotion of oxidase activity and concentration of benzoate presented was obtained as a bell shaped curve. In the concentration range of added benzoate effective for the promotion of oxidase activity of thus modified cytochrome c, the formation of CO-compound of the latter in reduced form could not yet be proved. Above 1.0M of benzoate, at which its CO-compound is now available, the percentage of the promotion of oxidase activity was decreased. This seems to be due to the decreased rate of the reduction of thus modified cytochrome c by ascorbate. A similar phenomenon could also be observed by salicylate, as already mentioned above.
The present authors wish to thank Prof. K. Kaziro for his encouragement and helpful discussions during the course of this work. This work was supported in part by the Scientific Research Fund of Ministry of Education for which we wish to express our thanks.抄録全体を表示PDF形式でダウンロード (874K) -
ISAMU SHIIO, BUNJI MARUO, SHIRO AKABORI1956 年 43 巻 6 号 p. 779-784
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Water-lysed extracts of a halophilic Pseudomonas strain No. 101 catalyzed the transamination reactions between α-ketoglutaric acid and L-aspartic acid, L-phenylalanine and L-tyrosine (maximum QTN: 1, 640, 2, 170 and 388, respectively).
2. These transaminases were not halophilic unlike alkaline phosphomonoesterase of this halophilic bacterium.
3. From the activity changes during heat-treatment and growth of the culture, it was suggested that at least two transaminases existed in the lysed extracts, one corresponding to aspartic acid and the other to phenylalanine.
The authors are indebted to Prof. F. Egami, Chemical Institute, Faculty of Science, Nagoya University, for the gift of the bacterial strain and also to Mr. M. Shiroishi, Food Research Institute, the Department of Agriculture and Forestry, for the preparation of pyridoxal phosphate.抄録全体を表示PDF形式でダウンロード (310K) -
VI. DIE PROTEOLYTISCHE AKTIVITÄT DES SOJA-LEGUMELINSVON YASUO TAZAWA, TOYOYASU HIROKAWA1956 年 43 巻 6 号 p. 785-795
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーDas Legumelin wurde aus ungekeimten Samen, Kotyledonen der Keimpflanzen und unreifen grünen Samen von Sojabohne in nativem Zustand dargestellt, und seine proteolytischen Wirkungen wurden mit den verschiedenen Substraten wie Gelatine, Glycinin, Asparagin, Hippursäure, Benzoylglycylglycin, Leucylglycin und Leucylglycylglycin im variierenden pH-Bereiche geprüft. Dabei hat es sich ergeben, daβ das Legumelin der Kategorie von Papain-oder Kathepsin-Proteinase angehört, und völlig frei von jedweder peptidatischen Wirkung dargestellt ist. Ausserdem wurde es festgestellt, daβ die Proteinase durch den Zusatz des Trypsininhibitors ihre Aktivität verliert, und daβ dies Enzymprotein in aktivem Zustand nie einer Autolyse unterliegt, aber nach der Denaturierung leicht ins geeignete Substrat für dasselbe Enzym verändert werden kann.抄録全体を表示PDF形式でダウンロード (730K) -
III. ISOLATION OF SUCCINIC MONOUREIDEKATASHI ICHIHARA, YUKIYA SAKAMOTO, HITOSHI SATANI, NAOTADA OKADA, SHIR ...1956 年 43 巻 6 号 p. 797-801
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Succinic monourede was isolated as the oxidative decomposition product of urocanic acid in the presence of EDTA to the extract of Pseudomonas aeruginosa.
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2. In spite of the complete disappear of urocanic acid, the yield of succinic monoureide is small. Other intermediary metabolites may therefore be expected.
3. Succinic monoureide is not further metabolized by the extract of Pseudomonas aeruginosa.抄録全体を表示PDF形式でダウンロード (268K) -
III. AMMONIUM AND POTASSIUM IONS AS THE ACTIVATOR OF BETA-TYROSINASEKATASHI ICHIHARA, HISAO YOSHIMATSU, YUKIYA SAKAMOTO1956 年 43 巻 6 号 p. 803-810
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Phenol, pyruvate and NH3 are produced from tyrosine by the β-tyrosinase, obtained from B. coli phenologenes, in an equimolar ratio simultaneously.
2. Pyridoxal phosphate is the coenzyme of the β-tyrosinase.
3. For this reaction, the presence of NH4+ or K+ is required and Na+ or Li+ counteract K+ or NH4+ activation. The previous report was thus corrected.
4. This enzyme is inhibited by metal chelating compounds. Therefore, the participation of a certain heavy metal is suggested.
5. This enzyme is also inhibited by various SH reagents, and regains its activity by the addition of mercapto compounds. Concequently the participation of the SH group is suggested.
6. The mechanism of the enzymatic reaction is discussed.抄録全体を表示PDF形式でダウンロード (381K) -
SHIN FURIYA, TETSUO IZUMITANI, SHIGERU AKAMATSU1956 年 43 巻 6 号 p. 811-820
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーThe phosphodiesterase of snake (Habu) venom partly purified free from monoesterase liberates p-nitrophenol from ethyl ester of p-nitro-phenyl phosphoric acid. Ethyl phosphate itself is hydrolyzable by urine monoesterase. Combined action of those two enzymes produces ethyl phosphate as an intermediate, the amount of which increases to a maximum extent and then decreases. The form of curves indicating the decrease of the substrate, the increase and successive decrease of ethyl phosphate, and the progressive increase of free inorganic phos-phate, respectively, were discussed, taking into consideration the affinities of the substrate and its hydrolytic products to each of the two enzymes. The experimental data agreed fairly well with the values calculated applying those affinity constants and the activities of the enzyme solutions applied.
Some methods for the estimation of the dissociation constants of enzyme-inhibitor compounds were tested with satisfactory results.
The work was aided by a Grant in Aid of Scientific Research from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (564K) -
KATASHI ICHIHARA, YUKIYA SAKAMOTO, HIROSHI WADA, NAOTADA OKADA, KIN-IC ...1956 年 43 巻 6 号 p. 821-826
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. From 5-Hydroxytryptophan the substituted indole was not formed by the extract of E. colt but did not inhibit indole formation from tryptophan. The substituted indole was formed from 5-methyltryptophan.
2. 5-Methyltryptophan was decomposed by the liver extract of rabbit and the cell suspension of Y-strain and gave a kynurenine reaction, but 5-hydroxytryptophan was not decomposed and rather inhibited the kynurenine formation from tryptophan.
3. 5-Methylindole and 7-hydroxyindole were decomposed completely by the Y-strain. Pyrrole, 2-methyl-3-carbethoxypyrrole, 2-methyl-3-carboxypyrrole and 1-methylindole were not decomposed. The benzene nucleus of indole must be important for the opening of pyrrole ring. It was suggested, that the benzene moiety of indole can not be cleaved before the pyrrole ring opens.抄録全体を表示PDF形式でダウンロード (363K) -
I. EFFECTS OF ALKALI-TREATED RIBONUCLEIC ACID IN THE HEMOLYSIN FORMATIONKENTARO TANAKA, SHIZUE MAEKAWA, TAKAYOSHI HAYASHI, YOSHIRO KUROIWA1956 年 43 巻 6 号 p. 827-832
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. The hemolysin-forming potencies of the differently alkali-hydrolyzed nucleic acids were studied.
2. In the early stages of alkaline hydrolysis slight enhancement of the potency was observed.
3. Through acetone fractionation of unhydrolyzed nucleic acid and of that briefly treated with alklai, the fractions were tested for hemoly-sin-forming potency. In both cases, it appears that only rather large molecules have such potency.
The authors wish to thank Prof. F. Egami for his helpful suggestions in this work. It is a pleasure to acknowledge several helpful discussions with Dr. Y. Yagi.抄録全体を表示PDF形式でダウンロード (322K) -
II. EFFECTS OF RIBONUCLEASE DIGESTION OF RIBONUCLEIC ACID ON THE HEMOLYSIN FORMATIONKENTARO TANAKA, SHIZUE MAEKAWA, TAKAYOSHI HAYASHI1956 年 43 巻 6 号 p. 833-840
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. The hemolysin-forming potency of the RNase-hydrolyzed RNA was studied in some detail.
2. The enhancement of the potency by the RNase hydrolysis was confirmed.
3. The hemolysin-forming potency of alkali-hydrolyzed RNA was also enhanced by the RNase hydrolysis.
4. The hemolysin-forming potency of the slightly alkali-prehy-drolyzed fraction which is almost inactive and is composed of smaller molecules was also enhanced by RNase hydrolysis.
5. Comparison between the potencies of alkali-hydrolyzed RNA and of RNase-hydrolyzed RNA having nearly the same degradation degree, showed that the remarkable enhancement of hemolysin-forming potency by RNase hydrolysis is closely connected with the specificity of the RNase.
The authors wish to thank Prof. F. Egami for his encouraging interest and advices during the course of this work.抄録全体を表示PDF形式でダウンロード (390K) -
II. EFFECT OF AMINO ACID ANALOGUES ON AMYLASE FORMATION BY BACILLUS SUBTILIS; AN APPARENT COMPETITION BETWEEN AMYLASE FORMATION AND NORMAL CELLULAR PROTEIN SYNTHESISMASAYASU NOMURA, JUNKO HOSODA, BUNJI MARUO, SHIRO AKABORI1956 年 43 巻 6 号 p. 841-850
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーAmylase formation by washed cell suspensions of Bacillus subtilis H was studied. A glucose and amino acid mixture, while it promoted normal cellular protein synthesis, showed no promoting effect on amylase formation during an initial period of 3 to 4 hours.
The addition of ethionine did not inhibit amylase formation, although it greatly inhibited the incorporation of S35-methionine into cellular protein. Sometimes even a considerable activating effect was observed when ethionine was added alone or together with the glucose and amino acid mixture. Other analogues tested, 5-methyltryptophan and norleucine, also did not inhibit amylase formation.
Active factors, prepared from boiled cell extract, markedly promoted the formation of amylase. This process was greatly inhibited by the addition of the glucose and amino acid mixture. This inhibition was partially suppressed by the addition of amino acid analogues (ethionine plus norleucine).
The concept of competition between amylase formation as a kind of abnormal biosynthesis and normal cellular protein synthesis was proposed and discussed.抄録全体を表示PDF形式でダウンロード (555K) -
I. THE PREPARATION OF L-PHENYLALANINESETSUJI SAKURAI1956 年 43 巻 6 号 p. 851-855
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. Phenylpyruvic acid was prepared by decarboxylation of phenyl-oxalacetic acid obtained by heating ethyl phenyloxalacetate with acetic acid and p-toluenesulfonic acid.
2. L-Phenylalanine was obtained from phenylpyruvic acid by heart muscle transaminase in an yield of 64 per cent.抄録全体を表示PDF形式でダウンロード (276K) -
KAZUO OKUNUKI, HIROSHI MATSUBARA, SHIGEHIKO NISHIMURA, BUNJI HAGIHARA1956 年 43 巻 6 号 p. 857-866
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーThe specificity of crystalline bacterial proteinase has been investigated by the fluorodinitrobenzene and hydrazinolysis-DNP methods. Oxidized lysozyme was mainly used as the substrate and other proteins, such as bacterial α-amylase and proteinase itself, were also used. It was found that this proteinase has a broad specificity and mainly attacks both N- and C-sides of neutral and acidic amino acid residues and others partially in the proteins.
The authors express their many thanks to Prof. S. Akabori, Dr. K. Ohno, Mr. T. Ikenaka, and Mr. I. Haruna for the kind technical guidance and to Mr. T. Seki for his kind advice on the technique of ion exchange resin chromatography and also to Nagase Co. Ltd., for the kind supply of the crystalline bacterial proteinase.抄録全体を表示PDF形式でダウンロード (622K) -
TAMIO YAMAKAWA1956 年 43 巻 6 号 p. 867-874
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリー1. From human and equine delipidized blood stroma, acetylated form and glycolated form of sialic acids, were obtained, respectively.
2. Acetylated form of sialic acid was also obtained from human and equine serum.
3. The analysis of these substances was presented.
The author wishes to thank Mr. B. Kurihara and Miss R. Qta for C, H and N analyses.抄録全体を表示PDF形式でダウンロード (457K) -
1956 年 43 巻 6 号 p. e1
発行日: 1956年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (43K)
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