巻号一覧
44 巻, 7 号
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SHIGEHARU NAGASAWA1957 年 44 巻 7 号 p. 399-411
発行日: 1957/07/25
公開日: 2008/11/18
ジャーナル フリー1. The esters accumulated in the mucosa of the rat small intestine during the absorption of the sugar given were identified as follows by means of paper chromatography combined with radioautography:
a. Glucose: G-1-P, G-6-P and F-1, 6-diP.
b. Fructose: G-6-P, F-1, 6-diP, F-1-P and an unknown ter.
c. Galactose: could not be decided.
2. G-1-P, G-6-P and F-1, 6-diP were also found each in an appreciable amount in the intestinal mucosa in rats which had been starved for 24 to 48hours.
3. The existence of phosphoglucomutase in the mucosa of rat small intestine was confirmed.
4. ATP accelerated the conversion of G-6-P to G-1-P by reversing the equilibrium.
5. Glycogen content in the mucosa during hexose-absorption increased.
6. The rate at which different sugar phosphates were split by the purified intestinal phosphatase was varied in this decreasing order:
G-1-P>β-glycerophosphate>F-1, 6-diP>G-6-P.
7. The amount in which three phosphorylated sugars, G-l-P, G-6-P and F-1, 6-diP, were absorbed in a difinite time was varied in the follow-ing order:
G-1-P>G-6-P>F-1, 6-diP.
I wish to thank Prof. Dr. R. Hirohata, director of the Department, for sug-gesting this problem, and for his valuable advice and encouragement throughout the investigation. Many thanks are also due to Assist. Prof. Dr. J. Nagai, for his encouraging interest during the course of this work.
The present work was partly aided by the Scientific Research Fund of Ministry of Education.抄録全体を表示PDF形式でダウンロード (1140K) -
VII. PURIFICATION OF THE CYTOCHROME OF SULFATE-REDUCING BACTERIA AND ITS PHYSIOLOGICAL ROLEMAKOTO ISHIMOTO, JIRO KOYAMA, TATSUHIKO YAGI, MASARU SHIRAKI1957 年 44 巻 7 号 p. 413-423
発行日: 1957/07/25
公開日: 2008/11/18
ジャーナル フリーCytochrome of sulfate-reducing bacteria, strict anaerobes, was purified by ion-exchange chromatography. Purified cytochrome has absorption maxima at 553, 525 and 419mμ in its reduced form and 410 and 350mμ in its oxidized form. Absorption at 280mμ is very low. Iron contents are determined to be 0.9 per cent. Reconstruction studies with the cytochrome and the extracts deprived of the cytochrome revealed its participation as the intermediary electron carrier between hydrogenase and thiosulfate reductase.
The authors wish to thank Dr. T. Soda and Dr. S. Akabori for their kind guidance and Dr. H. Tamiya for his valuable advice throughout this investigation. They are also grateful to Mr. Y. Sugiura for his technical assistances. The expences for the work were aided partly by the Scientific Research Funds from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (561K) -
ISAO YAMAZAKI1957 年 44 巻 7 号 p. 425-432
発行日: 1957/07/25
公開日: 2008/11/18
ジャーナル フリーIn the MB reducing activity of TP in the presence of RnH2, the molar ratio of reduced MB to added H2O2 becomes unity under the experimental conditions in which added H2O2 is used most effectively for the reduction of MB. This fact suggests the following mechanism
2 RnH2+H2O2TP→2RnH+2H2O (1)
2 RnH+MB→2Rn+MBH2 (2) that is;
2RnH2+H2O2+MBTP→2Rn+MBH2+2H2O (3)
The reaction of Equation I has been demonstrated by the experiments of George and Chance and the reducing ability of free radical RnH•has also been confirmed.抄録全体を表示PDF形式でダウンロード (341K) -
II. CHARACTERIZATION OF GLUCURONIDES IN HUMAN URINE AFTER ADMINISTRATION OF PARA-AMINO-SALICYLATEMAKOTO NAKAO, ISAMU YANAGISAWA1957 年 44 巻 7 号 p. 433-441
発行日: 1957/07/25
公開日: 2008/11/18
ジャーナル フリーThe occurrence of five different glucuronides, ester glucuronide and ether-glucuronide of PAS, the both types of glucuronides of N-acetyl PAS, and presumably an ether glucuronide of oxidation product of PAS, in human urine after ingestion of PAS were demonstrated by paper chromatography in combination with enzymatic hydrolysis.
The authors expresse their thanks to Prof. Yoshikawa for his constant leading and are indebted to Mr. Horie for the use of paper electrophoresis. This work was aided by a Grant from the Scientific Research Fund of the Ministry of Education.抄録全体を表示PDF形式でダウンロード (523K) -
I. SIZE AND SHAPE DETERMINATION BY THE MEASUREMENT OF SEDIMENTATION CONSTANT, DIFFUSION COEFFICIENT, AND VISCOSITYTOSHIZO ISEMURA, SHIZUE FUJITA1957 年 44 巻 7 号 p. 443-451
発行日: 1957/07/25
公開日: 2008/11/18
ジャーナル フリー1. The molecular weight and molecular shape of Taka-amylase A were studied by the measurements of sedimentation constant, diffusion coefficient and viscosity.
2. If we assume the molecule of a prolate ellipsoid of revolution of axial ratio 4.00 without or with scarce hydration, the molecular weight would be 51, 000, which is in good agreement with previous data. The length of the major axis of the molecule is 125 Å and that of the minor axis 30 Å, respectively.
3. The partial specific volume of Taka-amylase A is 0.700 and less than the calculated value from the specific volumes of constitutional amino acids.
In conclusion, the authors express their hearty thanks to Prof. S. Akabori and Mr. T. Ikenaka, of the Faculty of Science, Osaka University who supplied us the valuable sample of Taka-amylase and the authors are also indebted Prof. K. Fukai of the Institute for Microbiological Desease, Osaka University for his kind guidance for taking the photographs of sedimentation diagram.抄録全体を表示PDF形式でダウンロード (487K) -
I. PURIFICATION PROCEDURES AND PROTEINASE DIGESTION OF BAKER'S YEAST CYTOCHROME CMITSUHIRO NOZAKI, TATEO YAMANAKA, TAKEKAZU HORIO, KAZUO OKUNUKI1957 年 44 巻 7 号 p. 453-464
発行日: 1957/07/25
公開日: 2008/11/18
ジャーナル フリー1. Cytochrome c prepared from baker's yeast without using TCA indicates several chromatographic species on cation exchangers.
2. Though yeast cytochrome c is modified by treatments with TCA and heat in respect to chromatographic species and digestability by bacterial proteinase, its reducibility by yeast lactic dehydrogenase is not changed by these treatments.
3. It was established by the bacterial proteinase method that yeast cytochrome c is more stable to digestion by bacterial proteinase in its reduced form than in its oxidized form.
4. The bovine and horse cytochrome c prepared with the use of TCA are markedly less susceptible to the bacterial proteinase even in their oxidized form than yeast cytochrome c in its reduced form prepared without use of TCA.
Based on these facts, mode of existence of yeast cytochrome c in the living cells was descussed.
The authors wish to express their thanks to Oriental Yeast Co., Ltd. for source-material.抄録全体を表示PDF形式でダウンロード (636K) -
SHINJI ISHIKAWA, KYOKO NOGUCHI1957 年 44 巻 7 号 p. 465-469
発行日: 1957/07/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (257K)
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