巻号一覧
44 巻, 9 号
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KOSCAK MARUYAMA, HIROYUKI MATSUMIYA1957 年 44 巻 9 号 p. 537-542
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリーThe contractile protein from starfish tube-foot muscles showed res-ponses to ATP. It had an ATPase action, which was enhanced by Ca and to a less extent by Mg. ATP caused the so-called superprecipitation in 0.1M KCI and drop in light-scattered intensity in the presence of 0.6M KCl and 10mM MgCl2.
We wish to thank Assist. Prof. Y. Tonomura of Hokkaido University for his warm encouragements and also for his helpful criticism.抄録全体を表示PDF形式でダウンロード (622K) -
I. INHIBITION OF FERMENTATION BY PHOSPHATEMASAYA HAYASHIBE1957 年 44 巻 9 号 p. 543-556
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリー1. When some kinds of bakers' yeast cells were placed in phosphate-containing sugar solution, the rate of fermentation increased intensely at the initial stage and then rapidly decreased at a later stage, but the rate of respiration was not affected.
2. This fermentation inhibition was scarcely observed in low pH ranges.
3. The cells that accepted fermentation inhibition by phosphate did not recover the original fermentation rate on being merely washed of the external phosphate.
4. This phenomenon occurred not only in the phosphorus-poor cells but in the phosphorus-rich cells after phosphate starvation. The phosphorus-poor cells accumulated phosphate within the cells as meta-phosphate during fermentation. 2, 4-Dinitrophenol and sodium azide blocked this fermentation inhibition, which indicated the intimate relation between metaphosphate formation and fermentation inhibition.
5. Metal ions such as magnesium, calcium, and zinc counteracted this inhibition at the stage of metaphosphate accumulation, thereafter only magnesium released from this inhibition and calcium and zinc did not indicate any action.
6. It was inferred from the above observation that metaphosphate formed during fermentation had combined with magnesium, by which the rate of fermentation decreased.
The author would like to thank Mr. T. Shoda, the Director of Oriental Yeast Manufacturing Company, who gave him the opportunity to publish this report. The author is grateful for continued encouragement of Mr. K. Miyakawa, the chief of Research Department, and Dr. S. Nishimura, the former chief of this Department. The author wishes also to express his sincere gratitude to Mr. T. Matsuo for his technical assistance.抄録全体を表示PDF形式でダウンロード (700K) -
III. THE PREPATATION OF L-METIONINESHIGEKI YAMADA, SETSUJI SAKURAI1957 年 44 巻 9 号 p. 557-564
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリー1. α-Keto-γ-methylmercaptobutyric acid, keto analogue of methio-_??_ FIG. 2. Chromatogram of 2, 4-dinitrophenyl-methionine.
Column and developing solvent; The same as in Fig. 1.
Band A; 2, 4-Dinitrophenyl-methionine sulfoxide (9).
Band B; 2, 4-Dinitrophenyl-methionine.
No. 1; 3 μM of 2, 4-dinitrophenyl-methionine.
No. 2; 3 μM of 2, 4-dinitrophenyl-methionine was dissolved in 5ml.
of commercial ether and dried in vacuo.
No. 3; The same conditions as in No. 2 except that purified ether was used.
No. 4; The same conditions as in No. 2 except that commercial ethyl acetate was used instead of ether.
No. 5; The same conditions as in No. 4 except that purified ethyl acetate was used.
No. 6; 3 μM of methionine was reacted with 2, 4-dinitrofluorobenzene and the reaction mixture was extracted with commercial ethyl acetate and then the solution was dried in vacuo.
N. 7; The same conditions as in No. 6 except that purified ethyl acetate was used.
No. 8; 2, 4-Dinitrophenyl-methionine sulfoxide.
nine, was prepared by hydrolysis of methyl α-methoxalyl-γ-methyl-mercaptopropionate with dilute hydrochloric acid.
2. L-Methionine was obtained from ketomethionine by heart muscle transaminase in an yield of 46 per cent based on the ketomethio-nine used.
3. The effect of peroxide contained in commercial solvents on the determination of ketomethionine or methonine was described.
The author's best thanks are due to Prof. S. Akabori for his continued interest and advice.抄録全体を表示PDF形式でダウンロード (432K) -
SHOSHICHI NOJIMA, NOBUYUKI UTSUGI1957 年 44 巻 9 号 p. 565-573
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリー1. A new procedures with dinitrofluorobenzene using xylene as the fractionating solvent have been introduced in the method for the estimation of lipid-ethanolamine and -serine in animal tissues.
2. Lipid-choline, -ethanolamine and -serine have been determined in several tissues of rats and pig.
The authors wish to express their gratitude to Dr. D. Mizuno, Chief of the De-partment of Biochemistry, National Institute of Health, Tokyo, Prof. Dr. T. Ukita, Tokyo University, and Assistant Prof. Dr. Y. Yamakawa, Institute for Infectious Diseases, Tokyo, for their constant interests and encouragements throughout this work.抄録全体を表示PDF形式でダウンロード (513K) -
ON THE PURIFICATION OF THE REACTION PRODUCTS AND THEIR SPECTROSCOPICAL PROPERTIESKOOZOO KAZIRO, KEIZOO TSUSHIMA, FUJIKO UCHIMURA1957 年 44 巻 9 号 p. 575-585
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリー1. Two intermediary products of heme decomposition in the system of dicyanhematin-ascorbic acid-hydrogen peroxide were isolated. They were designated tentatively as 588- and 618-heme from their spectroscopic properties.
2. The millimolar extinction coefficients of dicyanhemochromes and dipyridine hemochromes derived from 588- and 618-heme purely isolated were determined as ε588mM=14.73, ε580mM=1 7.2, ε618mM=10.02, and ε608mM=9.48.
3. By detachment of iron from these two hemes, 588- and 618-porphyrin were prepared and purified. Absorption maxima of 588-porphyrin dimethylester in 20 per cent HC1 solution were at 624, 573 and (520)mμ. Those of 618-porphyrin dimethylester were at (690), 643, 588 and (540)mμ. In chloroform solution, 588-porphyrin showed its maxima at 640, 586, 554 and 514 mμ, while 618-porphyrin at 658, 605, 570 and 525mμ.
The spectroscopical properties of these two products and their de-rivatives are summarized in Table I.
4. No biliverdin nor its precursor could be obtained from 588-heme nor 618-heme in the system of their pyridine-hemochrome-ascorbic acid-hydrogen peroxide.
5. In the present reaction, 588-heme has been proved to be the immediate precursor of 618-heme.
The present work was aided in part by the grant of Scientific Research Fund of the Ministry of Education for which the authors wish to thank.抄録全体を表示PDF形式でダウンロード (607K) -
V. DENATURATION AND INACTIVATION OF YEAST ALCOHOL AND MUSCLE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASESICHIRO SEKUZU, BUNJI HAGIHARA, FUMIO HATTORI, TAKESHI SHIBATA, MITSUHI ...1957 年 44 巻 9 号 p. 587-594
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリーInactivations and denaturations of yeast ADH and muscle GDH were investigated in the presence of urea and two SH inhibitors, MIA and PCMB. The “ratio of denaturation” (“RD”) and the “ratio of inactivation” (“RI”) almost agreed with one another at any stage in the duration of urea treatment. In the case of PCMB treatment, however, “RI” was larger than “RD”, while, with MIA, denaturation was hardly observed in spite of the strong inactivation.抄録全体を表示PDF形式でダウンロード (436K) -
VI. EFFECT OF DIPHOSPHOPYRIDINE NUCLEOTIDE ON DENATURATION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASEMITSUHIRO NOZAKI, ICHIRO SEKUZU, BUNJI HAGIHARA, TAKASHI YONETANI, MAS ...1957 年 44 巻 9 号 p. 595-600
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリーEffect of DPN+ against GDH in the presence of urea, MIA or PCMB was studied using the proteolytic method presented by us. Denaturation of GDH caused by urea or PCMB was markedly decreased by the presence of DPN+, whereas inactivation caused by PCMB or MIA was not influenced by it. Based on the above results, the protec-tive effect of intramolecular change of GDH caused by the addition of DPN+ was discussed in connection with the difference of the mode of action of SH inhibitors.抄録全体を表示PDF形式でダウンロード (304K) -
VII. EFFECT OF COENZYME AND SUBSTRATES ON DENATURA-TION OF DEHYDROGENASESICHIRO SEKUZU, JINPEI YAMASHITA, MITSUHIRO NOZAKI, BUNJI HAGIHARA, TAK ...1957 年 44 巻 9 号 p. 601-614
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリーExtensive studies were carried out in an attempt to clarify whether the two DPN-linked dehydrogenases, lactic and alcohol dehydrogenases, are stabilized in the presences of substrates, DPN, and their related compounds. Using the bacterial proteinase method for the determina-tion of the ratio of denaturation and the amperometric titration methodfor the measurement of the number of reactive SH-groups in enzyme protein, it was demonstrated that the inactivation and denaturation of LDH in a solution of urea proceeded in parallel. Under this condition, both dehydrogenases were considerably protected by DPN+ and DPNH, and slightly by some substrates. When some of the substrates and DPN were coupled, the protection against urea denaturation was marked-ly enhanced. The combination of DPN+ and pyruvate protected LDH from the denaturation markedly, but not ADH. The latter enzyme, on the other hand, was protected by the combination of DPN+ and ethyl alcohol. While ATP also protected LDH at a higher concentra-tion from the action mentioned above, the protection was not increased by the addition of pyruvate.
The stabilizing effect of DPN+ and DPNH on both enzymes was also essentially confirmed in the case of heat denaturation.
Based on the above results, the mechanism of the stabilization of DPN-linked dehydrogenases protein was discussed.抄録全体を表示PDF形式でダウンロード (744K) -
MASAKIYO KONO, SHIGEHIKO TANIGUCHI, FUJIO EGAMI1957 年 44 巻 9 号 p. 615-618
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (179K) -
FUJIO EGAMI, MAKOTO ISHIMOTO, TAKESHI MORI, YASUYUKI OGURA, KAZUO OKUN ...1957 年 44 巻 9 号 p. 619-622
発行日: 1957/09/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (224K)
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