巻号一覧
45 巻, 1 号
選択された号の論文の8件中1～8を表示しています
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YUKIYA SAKAMOTO, TOMOJI MITSUHASHI, KATASHI ICHIHARA1958 年 45 巻 1 号 p. 1-7
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリー1. In the intact rabbit and its liver extract, in the presence of nitroso-R salt, the formation of GA from HGA, GAL, DPGCA and DPGOA was demonstrated respectively. In the case of HGA four spots beside that of substrate were found on the paperchromatogram which were identified as GA, GAL, DPGCA and DPGOA. The Rf 0.77 compound was remained unidentified, though it could be metabolized to GA.
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FIG 1. Paperchromatogram of the reaction mixture of HPAA by liver extract.
(1) GA
(2) SA
(3) HPAA
(4) HPAA 60 μM, liver extract 20ml.; pH 7.2, time 3 hrs.
(5) (4) plus nitroso-R salt:final concentration 10-3M;
Solvent:
isopropanol: NH4OH: water=8:1:1
Notice: The spots corresponding to GA, SA and HPAA respectively are easily distinguished by their behavior to the ultraviolet-light. GA shows bluish-white fluorescence, SA greenish-white to the ultraviolet-light, while HPAA absorbs the ultraviolet-light.
2. Among 10 metalcatchers tested, nitroso-R salt, oxine and o-phenanthroline were effective on the formation of GA, and seem to block the different point of this pathway individually. R-acid, G-acid, Schaeffer's acid and N-W-acid were proved to be inert.
3. Since the side chain of HPAA was not abbreviated to form SA, 2, 5-dihydroxy group of HGA is essential for the abbreviation of its acetate rest in the organism.
The authors are indebted to Dr. T. Sakan for synthesizing of GAL, DPGCA and DPGOA.抄録全体を表示PDF形式でダウンロード (364K) -
YASUHARU SEKIZAWA1958 年 45 巻 1 号 p. 9-11
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリーThe amide of trans-cinnamic acid (I) was recognized as the metabolite of an unidentified Streptonyces No. 902.
Author gratefully thanks to Prof. Dr. Y. Nakamura for his many kind advices and Mrs. Y. Baba, Tokyo Pharmaceutical University, for micro elementary analyses. Author was also indebted to Mr. M. Arishima for his advices in microbiological field, and to Mr. M. O gas a war a for the pilot plant fermentation and to Mrs. K. Harada for the skilful assistance.抄録全体を表示PDF形式でダウンロード (129K) -
KYUTARO SHIMIZU, TOSHIAKI USUI, TADASHI FUJIOKA, KAZUMI YAMASAKI1958 年 45 巻 1 号 p. 13-16
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリーHydroxamic acids of some bile acids were prepared and paper as well as column chromatography of these acids were presented, where those of deoxycholic and chenodeoxycholic acids were shown to be separable by the paper chromatography.抄録全体を表示PDF形式でダウンロード (224K) -
YOSHIE MURAKOSHI1958 年 45 巻 1 号 p. 17-25
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリーThe alkaline phosphomonoesterase is a magnesium-apoenzyme complex. In the dialyzed swine kidney autolysate, magnesium, zinc, manganese, calcium and iron were found but no copper.
A slight hydrolytic action, even without the addition of magnesium, is due to this ion present in the autolysate itself. Its concentration was calculated to be 4.8×10-6M in the hydrolyzing test solution through analysis of the activity curve and the value agreed approximately with that obtained by colorimetric analysis of the dialyzed autolysate.
Zinc ion is inhibitory to apoenzyme by competition with magnesium. Some chelating reagents, for instance, ethylenediamine capable of complex formation with zinc could slightly activate the enzyme of the dialyzed autolysate.
Polarographic estimation of zinc concentration in the autolysate revealed it to be 3.8×10-7M. This value nearly agreed with 6.9×10-7M, calculated from the point of competition with magnesium.
Manganese salt was effective for activation of enzyme at lower concentrations than magnesium. However, at its higher concentration an inhibition took place because of formation of inactive complex with the second manganese ion. The result of manganese analysis in the autolysate showed that the concentration of manganese in the test solution was so low that any activation of the enzyme could not take place.
The inhibition by copper was non-competitive with magnesium.
The author wishes to express his sincere thanks to Prof. S. Akamatsu for his kind guidance in this work. This investigation was supported by a Grant in Aid for Scientific Research from the Ministry of Education to Prof. S. Akamatsu.抄録全体を表示PDF形式でダウンロード (585K) -
II. DETERMINATION OF THE END GROUPS OF CLUPEINE AND SALMINE AND THEIR MOLECULAR WEIGHTTOSHIO ANDO, KOICHI IWAI, MASAMI KIMURA1958 年 45 巻 1 号 p. 27-39
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリー1. Methylesters of clupeine and salmine were prepared from purified sulfates and crude picrates of the protamines (derived from Clupea pallasii and Oruorhynchus keta, respectively) by two methods, in which esterification was carried out (A) by allowing them to stand at room temperature for prolonged periods of time in methanol containing 0.1 N hydrochloric acid, or (B) by short time treatment under cooling in methanol saturated with hydrogen chloride.
2. Methoxyl contents of the purified ester sulfates of both protamines were found to be 0.40-0.41 per cent in the products obtained by method A when esterified thoroughly, and 0.39-0.46 per cent by method B. Since these methoxyl groups are considered to be introduced only into C-terminal carboxvl groups of the protamine molecules, molecular weight of 6, 000-6, 200 (or 5, 400-6, 400 by method B) was calculated for both protamines from the result.
3. Free amino groups (N-terminal groups) of clupeine and salmine were determined by manometric Van Slyke method. Consideration was given to an amount of contribution from the guanidyl groups to the results. Thus clupeine was found to have an 0.5-0.9 equivalent of the amino group per molecule (molecular weight=6, 000), the remaining part appearing to be an imino group. Salmine was found to contain almost none of the amino group, hence it was supposed to have mainly an imino group in the N-terminus.
The authors are indebted to Drs. S. Ishii and M. Yamasaki for their valuable discussion and to Dr. C. Hashimoto for the methoxyl determination. The expense of this work was partly aided by the Scientific Research Grant from the Ministry of Education, to whom our thanks are also due.抄録全体を表示PDF形式でダウンロード (994K) -
GENKICHIRO TAKAGAKI, SHUSUKE HIRANO, YASUZO TSUKADA1958 年 45 巻 1 号 p. 41-48
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリーThe effects of inhibitors on the oxygen uptake, lactic acid formation and glucose utilization by guinea pig brain slices metabolizing aerobically with glucose as a substrate were studied. Inhibitions of oxygen uptake of brain slices under the normal conditions by some inhibitors were not so predominant, although under the effect of potassium they were very clearly observed. But if considered resting upon their effects on lactic acid formation and glucose utilization, the differences of the metabolic characters between in presence and in absence of the potassium effect were not clearly confirmed.
Many complicated factors to be considered upon understanding the results obtained in the brain slices using metabolic inhibitors were mentioned and discussed. From the considerations cited above, it might be thought that glucose would be metabolized aerobically through the glycolytic and citric acid cycle routes either in presence or in absence of the potassium effect in brain slices as seen in many other tissues.抄録全体を表示PDF形式でダウンロード (558K) -
I. MALTASE AND TRANSGLUCOSYLASE ACTIVITIES OF THE AMYLASETERUO SAWAI1958 年 45 巻 1 号 p. 49-56
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリーAmylase of Candida tropicalis var. japonica was inferred to have maltase and transglucosylase activities on the basis of the following experimental results:
Optimum pH's of these three enzyme activities were all 4.0 and activity ratios among them were always essentially constant even if the enzyme preparation was developed on an ion exchange resin column, treated with heat, acid or alkali, or fractionated by electrophoresis, or the yeast cells from which enzyme solutions were obtained were grown on different carbon sources.
The author wishes to express his hearty gratitude to Prof. T. Miwa, Prof. J. Fukumoto and Dr. T. Yamamoto for their kind advices and encouragement, and to Dr. M. A. J ermyn, Wood Textile Research Laboratory, Commonwealth Scientific and Industrial Research Organization, Australia, for his kindness in reviewing this manuscript. The major part of this work was carried out in the laboratory, Institute of Polytechnics, Osaka City University. The expenses of this work were in part defrayed by a Grant in Aid for Scientific Research from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (483K) -
II. CHANGES ON LEVELS OF AMINO ACIDS AND AMIDES DURING THE ASSIMILATION OF AMMONIA AND UREA BY NITROGEN-STARVED CELLSAKIHIKO HATTORI1958 年 45 巻 1 号 p. 57-64
発行日: 1958/01/25
公開日: 2008/11/18
ジャーナル フリー1. The process of assimilation of ammonia and urea added as the sole source of nitrogen was investigated with nitrogen-starved cells of Chlorella ellibsoidea.
2. The changes that occurred during the assimilation were followed with special reference to the levels of various amino acids and amides involved in the soluble nitrogen fraction of the cell, and the results obtained with ammonia were compared with those obtained with urea.
3. Tentative formulae were proposed for the pathway of assimilation of the nitrogen souces as follows:
assimilation of ammonia: ammonia→amide (mainly glutamine)→arginine→cell substance.
assimilation of urea: urea→arginine→cell substance.
4. Additional experimental data showing the role of arginine in the assimilatory processes in question were presented.
The author expresses his cordial thanks to Dr. H. Tamiyaand Dr. A. Takamiya of the University of Tokyo for their kind guidance and encouragement in carrying out this work. This work was supported by a Grant in Aid for Fundamental Scientific Research from the Ministry of Education to which the author extends his grateful thanks.抄録全体を表示PDF形式でダウンロード (571K)
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