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Volume 45, Issue 3
Displaying 1-10 of 10 articles from this issue
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IV. ON THE ALLIIN HOMOLOGUES IN THE VEGETABLESMOTONORI FUJIWARA, MASAO YOSHIMURA, SADAKO TSUNO, FUJIO MURAKAMI1958 Volume 45 Issue 3 Pages 141-149
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSStarting from the discovery of allithiamine formed from the extract of garlic and thiamine, we have discovered the distributions of alliin homologues in any other plants than allium plants.
The alliin homologue is contained mainly in the plants of allium species and crucifera.
Alliinase detected by Stol et al., is generally contained in the plants of allium species, but not in the other plants than this species, even if alliin homologues is contained.
The nutritional significance of garlic has been discussed.
The authors express their hearty gratitute to Dr. U. Miura, Kyoto University, for encouragement, to Dr. T. Matsukawa and Dr. S. Yurugi, Research Laboratory, Takeda Pharmaceutical Industries, Ltd., for offer of help in various ways.View full abstractDownload PDF (492K) -
I. EFFECT OF C6-D14 FATTY ACIDS ON THE TRYPTIC DIGESTION OF OVALBUMINMICHIYO KONDO1958 Volume 45 Issue 3 Pages 151-158
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. C8 to C14-fatty acids promote the tryptic digestion of ovalbumin, but C6-fatty acid does not at concentrations 0.1-0.4M.
2. Ovalbumin becomes susceptible to tryptic digestion by the addition of fatty acids nearly as much as by heat denaturation.
3. At higher concentration of fatty acids the tryptic digestion decreased for both heated and unheated ovalbumin.
4. The optimum concentrations of fatty acids for tryptic digestion of ovalbumin decrease with the increase of the carbon chain.
5. The laevo-rotatory power of ovalbumin solution increases by the addition of fatty acid. The effect differs from fatty acid to fatty acid.
6. The greatest change of optical rotation is obtained by C12 acid at 0.015M concentrations, indicating that C12 acid is most effective in a modification of protein structure. This fits in with the observation by tryptic digestion.
The author wishes to express her sincere thanks to Prof. S. Akabori and Assist. Prof. B. Maruo for their kind guidance and encouragement throughout this work.View full abstractDownload PDF (1350K) -
YASUHARU SEKIZAWA1958 Volume 45 Issue 3 Pages 159-162
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSIt was already reported that trans-cinnamic acid amide (1) and ethoxy-ethene-1, 2-dicarboamide (2) were isolated from the cultured broth filtrate of Streptomyces No. 902.
In this paper, the futher fractionation of the filtrate against HeLa cell was reported to give a new antitumour substance designated as lenamycin having the properties of a specific organic acid amide.View full abstractDownload PDF (700K) -
IV. RATE OF INCORPORATION OF DIETARY LABELED CALCIUM INTO GROWING BONESUSUMU TSURUFUJI1958 Volume 45 Issue 3 Pages 163-168
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSIt was shown that rats placed on a calcium-deficient diet could be kept in a well-nourished condition when sufficient amount of calcium carbonate was given to the rats with a stomach tube. Rats, being fed as described above, were given one dose of calcium carbonate labeled with Ca45 in place of non-labeled calcium carbonate and sacrificed at intervals of 24 hours. The amount of labeled dietary calcium taken up by their incisor and specific activity of serum calcium were measured. The amount of calcium deposited into one lower incisor tooth of a rat through the formation of new incisor mineral has been estimated and the estimated value agrees with the previously measured value by the procedure based on quite different principles.
Physicochemical exchange seems to take only a little part in the fixation of labeled calcium by the incisor of the rat when labeled calcium is given orally as calcium carbonate. In such a condition, supposing that overall incorporation of radioactive calcium into the incisor is due to the formation of new minerals, the proportion of radioactive calcium deposited through bone growth is estimated to be only 15.6 per cent of overall uptake of radioactive calcium by the skeleton. Another 84.4 per cent seems to be due to physicochemical exchange and biological reconstruction processes.View full abstractDownload PDF (379K) -
YOSHIHARU MARUYAMA, HIROMI MITUI1958 Volume 45 Issue 3 Pages 169-175
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSPyrocatechase adaptability of Pseudomonas was influenced markedly by the growth phase of the cells. The adaptability of the cells was weakst at the begining of log phase, gradually increased and reached to the maximum level at stationary phase.
Supplement of glutamic acid to young cells caused an increase in adaptability to the level of the old cells.
Among non-nitrogenous compounds tested α-ketoglutaric acid was particularly effective in increasing adaptability of young cells. Strangely enough, just aeration of young cells in phosphate buffer was effective.
Free amino acids of young and old cells were determined. Usually old cells contained a larger amounts of free amino acids than young cells. The aeration technique, however, did not stimulate the accumulation of free amino acids within the cells. The relationship between free amino acid pool and adaptability was discussed.
The authors wish to express their many thanks to Prof. S. Akabori, Prof. S. Funahashi and Dr. B. Maruo for their kind guidance and many useful suggestions throughout the present work.View full abstractDownload PDF (380K) -
YOICHI MARUYAMA1958 Volume 45 Issue 3 Pages 177-184
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSEmploying T2 phage/Escherichia coli strain B system, the mode of several antiviral substances on virus multiplication was investigated. The actions of these substances on virus and bacterial growth were compared quantitatively under identical experimental conditions as far as possible.
Correlation analysis of the results obtained revealed three typical types of mode of action of antiviral substances. In Type I, substances (cyanide and hydrogen peroxide) were found to exert parallel activities on virus and bacterial growth. On the other hand, Type II substances, such as gentisate and oxytetracycline, showed selective toxicity on virus growth, whereas Type III substance, represented by penicillin, showed selective activity on bacterial growth.
The author wishes to express his gratitude to Dr. T. Yanagita for his valuable suggestions and criticism during the course of this work.
He is indebted to the Ministry of Education for a Grant in Aid for Fundamental Scientific Research.View full abstractDownload PDF (438K) -
I. PREPARATION OF CRYSTALLINE PROTEINASE OF BAC. SUBTILISBUNJI HAGIHARA, HIROSHI MATSUBARA, MASASHI NAKAI, KAZUO OKUNUKI1958 Volume 45 Issue 3 Pages 185-194
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSA convenient method for the preparation of crystalline proteinase of B. subtilis using a cation exchange resin, Duolite C-10, was described. A solution of commercial enzyme preparation was passed through a column of the resin at neutral pH and, after washing the column, the adsorbed proteinase was eluted with alkaline solution containing a salt in high concentration in order to prevent elution of the coloring matter. From the colorless eluate, the enzyme was concentrated by salting out and acetone precipitation, and crystallized as needles or plates from acetone solution. The yield of the recrystallized enzyme was more than 25 per cent in activity.
The authors wish to express their many thanks to the Nagase & Co., Ltd., Osaka for the enzyme preparation and also to the Nippon Kiko Co., Ltd., Osaka, and The Scientific Reserch Institute Ltd. for the resins.View full abstractDownload PDF (1303K) -
I. PURIFICATION OF CYTOCHROMES FROM PSEUDOMONAS AERUGINOSATAKEKAZU HORIO1958 Volume 45 Issue 3 Pages 195-205
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSWater-soluble cytochromes from Pseudomonas aeruginosa have been purified by a simple, continuous procedure which consists of chromatographies on an ion exchanger, Duolite CS-101, and aluminium oxide, and ammonium sulfate fractionation. A cytochrome adsorbed on CS-101 has the absorption maxima at 620-630, 551, 548, 521-523, and 418 mμ in the reduced state (Pseudomonas cytochromeGB), and two cytochromes purified by chromatography on aluminium oxide have the absorption maxima at 551, 521, and 416 mμ (Pseudomonas cytochrome551), and at 554, 525, and 416 mμ (Pseu-domonas cytochrome554), in the reduced state, respectively. Further, a bluecoloured protein havinu an nhcnrntinn maximum at 62.5 mμ in the nxirdized state was purified (Pseudomonas blue protein) and when this protein was reduced, the absorption maximum disappeared. Attempts were made to extract other cytochromes present in the bacteria by other procedures.
The author wishes to express his great thanks to Dr. Y. Takeda for his kind supply of the bacterial strain and fruitful advice, and would like to thank his colleagues Messrs. H. Matsubara, T. Higashi, M. Nozaki, J. Yamashita, T. Yamanaka, and H. Mizushima (same address to the author's) for helpful discussions during the course of this work, and Messrs. K. Kusai and M. Nakai (Nagase Co., Ltd., Amagasaki Factory, Amagasaki) for co-works.View full abstractDownload PDF (569K) -
TOSHIKO NAKAO, MAKOTO NAKAO, TERUMI NAKAJIMA1958 Volume 45 Issue 3 Pages 207-209
Published: March 25, 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSDownload PDF (152K) -
1958 Volume 45 Issue 3 Pages e1
Published: 1958
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSDownload PDF (21K)
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