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SAKARU SUZUKI, NORIKO TAKAHASHI, FUJIO EGAMI
1959 Volume 46 Issue 1 Pages
1-10
Published: January 25, 1959
Released on J-STAGE: November 18, 2008
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1. A method of synthesis of
p-nitrophenyl S
35-sulfate was described.
2. Cell-free extract of the mucous gland of “Charonia lampas” has been found to incorporate radioactive sulfate into charoninsulfuric acid when incubated with
p-nitrophenyl S
35-sulfate.
3. Two arylsulfatases with different pH optimum (5.0 and 6.0) were found in the extract of mucous gland and separated by zone electrophoresis. These arylsulfatases were not able to transfer sulfate from phenol to carbohydrate.
4. Arylsulfatase of the liver of
Charonia lamfias was purified. The purified arylsulfatase preparation has been found to catalyze the incorpora-tion of S
35-sulfate to polysaccharide in the presence of a non-dialyzed preparation of charoninsulfuric acid and
p-nitrophenyl S
35-sulfate. This activity has not been observed with dialyzed charoninsulfuric acid. Some unknown factor may participate in the reaction.
A part of the expense of this study was defrayed by a grant from Seikagaku-Kenkyusho Ltd., to which our thanks are due. Some of the experiments were carried out in the Marine Biological Station of Nagoya University.
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IV. CRYSTALLIZATION OF BEEF KIDNEY CYTOCHROME C
BUNJI HAGIHARA, KUNIO TAGAWA, ICHIRO SEKUZU, ICHIRO MORIKAWA, KAZUO OK ...
1959 Volume 46 Issue 1 Pages
11-17
Published: January 25, 1959
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kidney tissues contain an impure protein which prevents sufficient purification of cytochrome c for crystallization by any of the methods reported for muscle tissues. This impurity possessing a similar affinity to the resin to that of oxidized cytochrome c was found to be removed by chromatography of the cytochrome solution in its reduced form permitting easy crystallization of the pigment. On the basis of this finding, two methods,
i.e., Methods A and B, were described for purification and crystallization of kidney cytochrome c. These two methods are the mo-difications of Method III (using direct adsorption) reported in the previous papers. In the latter method the yield of crystals was about 40mg. from 6kg. of kidneys (3kg. of cortex), while the former method gave a smaller yield because of modification of the pigment caused during the TCA precipitation. Crystals usually appeared as rosettes similar to those of beef heart cytochrome c and sometimes as hexagonal plates.
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I. ACTION OF α-CHYMOTRYPSIN ON AMINOACYL-L-TYROSINAMIDES
NOBUO IZUMIYA, TAKESHI YAMASHITA
1959 Volume 46 Issue 1 Pages
19-30
Published: January 25, 1959
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I. A number of aminoacyl-L-tyrosinamide hydrochlorides in which the aminoacyl substituents are the aliphatic amino acid groups have been synthesized and tested as substrates for α-chymotrypsin.
2. By the use of glycyl-L-tyrosinamide, the pH optimum of chymotryptic hydrolysis was found to be near 8.3.
3. The values of proteolytic coefficients in various initial substrate concentrations and
Cmax. were estimated, and were taken as measures of the relative susceptibility of hydrolysis of the substrates by chymotrypsin.
4. The rate of action of chymotrypsin was increased markedly by the presence of large side chain groups in the N-terminal L-amino acid residue of the substrate.
5. The presence of D-alanyl residue in the substrate rendered the sensitivebond more resistant to hydrolysis by chymotrypsin.
6. Chromatographic analysis of the incubation mixture proved that although L-alanyl-, L-aminobutyroyl-, L-norleucyl- and L-leucyl-L-tyrosinamide produce an unknown substance positive for ninhydrin in very slight extent, all the substrates are hydrolyzed significantly by chymotrypsin.
The authors wish to thank Drs. J. P. Greenstein, M. Winitz and S. M. Birnbaum for generous gift of the precious amino acids and crystalline enzyme, and Prof. S. Shibuy a for his interest in this study. They also thank Dr. A. Tanaka for his discussion in this study.
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II. SPECIFICITY OF RIBONUCLEASE T1
KIMIKO SATO-ASANO
1959 Volume 46 Issue 1 Pages
31-37
Published: January 25, 1959
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I. Guanosine-2', 3'-cyclic phosphate and 3'-guanylic acid are formed as the digestion products of yeast RNA by RNase T
1 at the initial and at the later stage respectively.
2. RNA digestion by RNase T
1 is incomplete, and a resistant fraction remains.
3. Mononucleotide and the terminal nucleotides of oligonucleotides produced by RNase T
1 are exclusively guanylic acid.
4. Consequently it is clear that RNase T
1 splits the secondary phosphate ester bound of guanosine-3'-phosphate involving the formation of guanosine-2', 3'-cyclic phosphate as an intermediate and producing guanosine-3'-phosphate.
The auther wishes to thank Prof. F. Egami for his helpful discussion and encoura-gement. She is indebted to Sankyo Co. Ltd. for the gift of “Takadiastase Sankyo”, and to Dr. E. Iwase for the gift of U. V. filter of Scientific Research Institute.
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II. 8-AZAGUANINE RESISTANT-AND 8-AZAXANTHINE RESISTANT STRAINS
TOMOHARU OKUDA, MIKIHISA SAWA
1959 Volume 46 Issue 1 Pages
39-41
Published: January 25, 1959
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The purine metabolism of the 8-azaguanine resistant and 8-azaxanthine resistant strains of
Leuconostoc mesenteroides P-60 were compared with that of the original strain. These resistant strains did not require preformed exogenous purines for their growth and were less sensitive to aminopterin and 6-mercaptopurine than the original strain. While the growth of the azaguanine-resistant strain was inhibited by 8-azaxanthine like the original strain, azaxanthine-resistant strain was not inhibited by 8-azaguanine.
The authors are grateful to Dr. Y. Miura and K. Kitahara, of the University of Tokyo, for their helpful advices and encouragements.
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II. SPECTRAL PROPERTIES OF CYTOCHROME A
ICHIRO SEKUZU, SHIGEKI TAKEMORI, TAKASHI YONETANI, KAZUO OKUNUKI
1959 Volume 46 Issue 1 Pages
43-49
Published: January 25, 1959
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1. Potassium cyanide at a final concentration of 0.01
M causes a shift of the γ-band of oxidized cytochrome a from 424mμ to 428mμ, but no shift of that of the reduced form.
2. The γ-band of reduced cytochrome a shifts from 444mμ to 430mμ in the presence of carbon monoxide, while there is a small change in the region of the α-band.
3. Nitric oxide can combine with reduced eytochrome a and absorption maxima appear at 430mμ, u and at 603mμ.
4. The oxygen complex of reduced eytochrome a has maxima at426-28mμ and 603mμ. The spectrum is clearly distinguishable from that of the oxidized form. In the presence of a small amount of cytochrome c, reduced cytochrome a shows a strong autoxidizability.
5. The function of cytochrome a as cytochrome oxidase is discussed on the basis of these findings.
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VI. ON THE RATES OF INCORPORATION OF P32 INTO ENDIVIDUAL PHOSPHOLIPIDS IN TISSUES
TOSHIO SAKAGAWA, TADASHI SHIMOJO, OSAMU MINARI
1959 Volume 46 Issue 1 Pages
51-57
Published: January 25, 1959
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1. The method reported by Dawson was investigated, and it was learned that it was able to determine the specific activities of phosphatidyl-choline, phosphatidylethanolamine, diphosphoinositide and phosphatidylserine in small P
32 labelled samples.
2. The method has been used to measure the incorporation of P
32 into individual phospholipids in the intact rat liver after intraperitoneal injection of labelled phosphate.
3. After 30 minutes of the P
32 in it was observed that the highest specific activity was in diphosphoinositide fraction, the specific activities of phosphatidylethanolamine and phosphatidylserine were about 3/4 of that of diphosphoinositide and the specific activity of phosphatidyl-choline was significantly low as 1/6, but there was little difference among the specific activities of individual phospholipids 12 hours after the injection.
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AKITANE MORI
1959 Volume 46 Issue 1 Pages
59-62
Published: January 25, 1959
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The synthesis of γ-amino-α-hydroaybutyric acid has been accomplished in the following manner: first, in order to protect the amino radical of γ-aminobutyric acid the acetylation of the radical is undertaken; and then its α-position is temporarily replaced by bromine. And finally bromine is replaced by hydroxy radical.
Acknowledgement is due to Prof. Jinnai for his encouragement and painstaking proof-reading and to Dr. Kurod a of Okayama Nutrists' College for his invaluable advices, in preparing this manuscript.
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SATORU MAKISUMI
1959 Volume 46 Issue 1 Pages
63-71
Published: January 25, 1959
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Paper chromatographic analyses of extracts from a fresh-water alga,
Cladophora sp., showed the presence of two Sakaguchi-positive substances, which was isolated by means of ion exchange resins. These substances were identified as L-arginine and L-arginyl-L-glutamine, a new compound. However, there was no evidence that L-arginyl-L-glutamic acid is present as a preformed peptide in the material.
The author wishes to express his gratitude to Prof. S. Shibuya for his kind guidance, and to Assistant Prof. N. Izumiya for his valuable suggestions. This work was sup-ported in part by a Grant in Aid for the Miscellaneous Scientific Research from the Ministry of Education.
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II. PAPER CHROMATOGRAPHICAL DETECTION OF N-METHYL-PYRIDINE IN URINE OF RABBIT-ORGANISMS DOSED WITH PYRIDINE
YOSHIO OKUDA
1959 Volume 46 Issue 1 Pages
73-78
Published: January 25, 1959
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1. This report is a study on the qualitative and quantitative micro-detection of
N-methylpyridine in urine, which was obtained from rabbit-organisms administrated with pyridine, the detection was done by paper chromatography and ultraviolet absorption with spectrophotometer.
2. The
N-methylpyridine (Methylpyridyl-ammoniumhydroxide) can be qualitatively micro-detected by means of the paper chromatograms colored with PABA-CNBr treatment, and the spot is pink-red, and giving
Rf values 0.4-0.5, run with solvent
n-butanol: acetic acid: water=4:1:2 or 4:1:5 (
v/v) in mixture.
3. In the rabbit,
N-methylpyridine has been excreted in urine during 5 days after pyridine was administrated, either in the case of injection, or
per os.
4. The ultra-violet absorption spectra of acetone eluted
N-methylpyridine in rabbit urine show the remarkable absorption maximum at 329 mμ in wave length (Fig. 2).
5. The quantitative estimation of
N-methylpyridine in urine of rabbit-organisms dosed with 0.5g. pyridine was due to the paper chromatograms and the absorbance of the ultra-violet absorption by a Beckman's spectrophotometer, were as follows:
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V. CONJUGATION OF SULFATE WITH PYRIDOXINE AND PYRIDOXAL IN RAT LIVER
TOKURO SATO, MIEKO YAMADA, TAEKO SUZUKI, TOMITARO FUKUYAMA, HARUHISA Y ...
1959 Volume 46 Issue 1 Pages
79-81
Published: January 25, 1959
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A study has been made of the biological sulfate conjugation with pyridoxine, pyridoxal and pyridoxamine. Pyridoxine and pyridoxal have been shown to be readily conjugated with sulfate in the presence of rat liver slices as well as homogenate.
The position of the conjugation was concluded to be in the phenolic hydroxyl group.
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I. PAPER-CHROMATOGRAPHIC SEPARATION OF ACID-SOLUBLE PHOPHORUS COMPOUNDS INCORPORATING P
HARUHISA YOSHIKAWA, MAKOTO NAKAO, KANJI MIYAMOTO, ISAMU YANAGISAWA
1959 Volume 46 Issue 1 Pages
83-89
Published: January 25, 1959
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Acid-soluble phosphorus compounds of human erythrocytes were analysed with the aid of paper chromatography and P
32. After 30 minute incubation of erythrocytes with P
32 at 37° at least 15 radioactive compounds were obtained, including ATP, ADP, AMP, G-6-P, F-1, 6-DP, 2, 3-diphosphoglyceric acid, and 3-phosphoglyceric acid as identified. The presence of ribose-5-phosphate was indicated. An adenine nucleotide with two stable and two labile phosphate groups was also found.
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KOZO NARITA, SHIRO AKABORI
1959 Volume 46 Issue 1 Pages
91-92
Published: January 25, 1959
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AKIRA TSUGITA, MASAYORI INOUE, SHIRO AKABORI
1959 Volume 46 Issue 1 Pages
93-95
Published: January 25, 1959
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TOKUJI KIMURA
1959 Volume 46 Issue 1 Pages
97-99
Published: January 25, 1959
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SHUNJI MIZUHARA, KYUICHIRO OKUDA, KENGO KURAHASHI, YOSHIYUKI IWADO
1959 Volume 46 Issue 1 Pages
101-102
Published: January 25, 1959
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SETSURO EBASHI, YASUO TOYOKURA, HIRONAO MOMOI, HIDEO SUGITA
1959 Volume 46 Issue 1 Pages
103-104
Published: January 25, 1959
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MAKOTO ISHIMOTO
1959 Volume 46 Issue 1 Pages
105-106
Published: January 25, 1959
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