巻号一覧
46 巻, 1 号
選択された号の論文の18件中1～18を表示しています
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SAKARU SUZUKI, NORIKO TAKAHASHI, FUJIO EGAMI1959 年 46 巻 1 号 p. 1-10
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリー1. A method of synthesis of p-nitrophenyl S35-sulfate was described.
2. Cell-free extract of the mucous gland of “Charonia lampas” has been found to incorporate radioactive sulfate into charoninsulfuric acid when incubated with p-nitrophenyl S35-sulfate.
3. Two arylsulfatases with different pH optimum (5.0 and 6.0) were found in the extract of mucous gland and separated by zone electrophoresis. These arylsulfatases were not able to transfer sulfate from phenol to carbohydrate.
4. Arylsulfatase of the liver of Charonia lamfias was purified. The purified arylsulfatase preparation has been found to catalyze the incorpora-tion of S35-sulfate to polysaccharide in the presence of a non-dialyzed preparation of charoninsulfuric acid and p-nitrophenyl S35-sulfate. This activity has not been observed with dialyzed charoninsulfuric acid. Some unknown factor may participate in the reaction.
A part of the expense of this study was defrayed by a grant from Seikagaku-Kenkyusho Ltd., to which our thanks are due. Some of the experiments were carried out in the Marine Biological Station of Nagoya University.抄録全体を表示PDF形式でダウンロード (430K) -
IV. CRYSTALLIZATION OF BEEF KIDNEY CYTOCHROME CBUNJI HAGIHARA, KUNIO TAGAWA, ICHIRO SEKUZU, ICHIRO MORIKAWA, KAZUO OK ...1959 年 46 巻 1 号 p. 11-17
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーkidney tissues contain an impure protein which prevents sufficient purification of cytochrome c for crystallization by any of the methods reported for muscle tissues. This impurity possessing a similar affinity to the resin to that of oxidized cytochrome c was found to be removed by chromatography of the cytochrome solution in its reduced form permitting easy crystallization of the pigment. On the basis of this finding, two methods, i.e., Methods A and B, were described for purification and crystallization of kidney cytochrome c. These two methods are the mo-difications of Method III (using direct adsorption) reported in the previous papers. In the latter method the yield of crystals was about 40mg. from 6kg. of kidneys (3kg. of cortex), while the former method gave a smaller yield because of modification of the pigment caused during the TCA precipitation. Crystals usually appeared as rosettes similar to those of beef heart cytochrome c and sometimes as hexagonal plates.抄録全体を表示PDF形式でダウンロード (933K) -
I. ACTION OF α-CHYMOTRYPSIN ON AMINOACYL-L-TYROSINAMIDESNOBUO IZUMIYA, TAKESHI YAMASHITA1959 年 46 巻 1 号 p. 19-30
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーI. A number of aminoacyl-L-tyrosinamide hydrochlorides in which the aminoacyl substituents are the aliphatic amino acid groups have been synthesized and tested as substrates for α-chymotrypsin.
2. By the use of glycyl-L-tyrosinamide, the pH optimum of chymotryptic hydrolysis was found to be near 8.3.
3. The values of proteolytic coefficients in various initial substrate concentrations and Cmax. were estimated, and were taken as measures of the relative susceptibility of hydrolysis of the substrates by chymotrypsin.
4. The rate of action of chymotrypsin was increased markedly by the presence of large side chain groups in the N-terminal L-amino acid residue of the substrate.
5. The presence of D-alanyl residue in the substrate rendered the sensitivebond more resistant to hydrolysis by chymotrypsin.
6. Chromatographic analysis of the incubation mixture proved that although L-alanyl-, L-aminobutyroyl-, L-norleucyl- and L-leucyl-L-tyrosinamide produce an unknown substance positive for ninhydrin in very slight extent, all the substrates are hydrolyzed significantly by chymotrypsin.
The authors wish to thank Drs. J. P. Greenstein, M. Winitz and S. M. Birnbaum for generous gift of the precious amino acids and crystalline enzyme, and Prof. S. Shibuy a for his interest in this study. They also thank Dr. A. Tanaka for his discussion in this study.抄録全体を表示PDF形式でダウンロード (479K) -
II. SPECIFICITY OF RIBONUCLEASE T1KIMIKO SATO-ASANO1959 年 46 巻 1 号 p. 31-37
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーI. Guanosine-2', 3'-cyclic phosphate and 3'-guanylic acid are formed as the digestion products of yeast RNA by RNase T1 at the initial and at the later stage respectively.
2. RNA digestion by RNase T1 is incomplete, and a resistant fraction remains.
3. Mononucleotide and the terminal nucleotides of oligonucleotides produced by RNase T1 are exclusively guanylic acid.
4. Consequently it is clear that RNase T1 splits the secondary phosphate ester bound of guanosine-3'-phosphate involving the formation of guanosine-2', 3'-cyclic phosphate as an intermediate and producing guanosine-3'-phosphate.
The auther wishes to thank Prof. F. Egami for his helpful discussion and encoura-gement. She is indebted to Sankyo Co. Ltd. for the gift of “Takadiastase Sankyo”, and to Dr. E. Iwase for the gift of U. V. filter of Scientific Research Institute.抄録全体を表示PDF形式でダウンロード (835K) -
II. 8-AZAGUANINE RESISTANT-AND 8-AZAXANTHINE RESISTANT STRAINSTOMOHARU OKUDA, MIKIHISA SAWA1959 年 46 巻 1 号 p. 39-41
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーThe purine metabolism of the 8-azaguanine resistant and 8-azaxanthine resistant strains of Leuconostoc mesenteroides P-60 were compared with that of the original strain. These resistant strains did not require preformed exogenous purines for their growth and were less sensitive to aminopterin and 6-mercaptopurine than the original strain. While the growth of the azaguanine-resistant strain was inhibited by 8-azaxanthine like the original strain, azaxanthine-resistant strain was not inhibited by 8-azaguanine.
The authors are grateful to Dr. Y. Miura and K. Kitahara, of the University of Tokyo, for their helpful advices and encouragements.抄録全体を表示PDF形式でダウンロード (130K) -
II. SPECTRAL PROPERTIES OF CYTOCHROME AICHIRO SEKUZU, SHIGEKI TAKEMORI, TAKASHI YONETANI, KAZUO OKUNUKI1959 年 46 巻 1 号 p. 43-49
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリー1. Potassium cyanide at a final concentration of 0.01M causes a shift of the γ-band of oxidized cytochrome a from 424mμ to 428mμ, but no shift of that of the reduced form.
2. The γ-band of reduced cytochrome a shifts from 444mμ to 430mμ in the presence of carbon monoxide, while there is a small change in the region of the α-band.
3. Nitric oxide can combine with reduced eytochrome a and absorption maxima appear at 430mμ, u and at 603mμ.
4. The oxygen complex of reduced eytochrome a has maxima at426-28mμ and 603mμ. The spectrum is clearly distinguishable from that of the oxidized form. In the presence of a small amount of cytochrome c, reduced cytochrome a shows a strong autoxidizability.
5. The function of cytochrome a as cytochrome oxidase is discussed on the basis of these findings.抄録全体を表示PDF形式でダウンロード (289K) -
VI. ON THE RATES OF INCORPORATION OF P32 INTO ENDIVIDUAL PHOSPHOLIPIDS IN TISSUESTOSHIO SAKAGAWA, TADASHI SHIMOJO, OSAMU MINARI1959 年 46 巻 1 号 p. 51-57
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリー1. The method reported by Dawson was investigated, and it was learned that it was able to determine the specific activities of phosphatidyl-choline, phosphatidylethanolamine, diphosphoinositide and phosphatidylserine in small P32 labelled samples.
2. The method has been used to measure the incorporation of P32 into individual phospholipids in the intact rat liver after intraperitoneal injection of labelled phosphate.
3. After 30 minutes of the P32 in it was observed that the highest specific activity was in diphosphoinositide fraction, the specific activities of phosphatidylethanolamine and phosphatidylserine were about 3/4 of that of diphosphoinositide and the specific activity of phosphatidyl-choline was significantly low as 1/6, but there was little difference among the specific activities of individual phospholipids 12 hours after the injection.抄録全体を表示PDF形式でダウンロード (623K) -
AKITANE MORI1959 年 46 巻 1 号 p. 59-62
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーThe synthesis of γ-amino-α-hydroaybutyric acid has been accomplished in the following manner: first, in order to protect the amino radical of γ-aminobutyric acid the acetylation of the radical is undertaken; and then its α-position is temporarily replaced by bromine. And finally bromine is replaced by hydroxy radical.
Acknowledgement is due to Prof. Jinnai for his encouragement and painstaking proof-reading and to Dr. Kurod a of Okayama Nutrists' College for his invaluable advices, in preparing this manuscript.抄録全体を表示PDF形式でダウンロード (155K) -
SATORU MAKISUMI1959 年 46 巻 1 号 p. 63-71
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーPaper chromatographic analyses of extracts from a fresh-water alga, Cladophora sp., showed the presence of two Sakaguchi-positive substances, which was isolated by means of ion exchange resins. These substances were identified as L-arginine and L-arginyl-L-glutamine, a new compound. However, there was no evidence that L-arginyl-L-glutamic acid is present as a preformed peptide in the material.
The author wishes to express his gratitude to Prof. S. Shibuya for his kind guidance, and to Assistant Prof. N. Izumiya for his valuable suggestions. This work was sup-ported in part by a Grant in Aid for the Miscellaneous Scientific Research from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (402K) -
II. PAPER CHROMATOGRAPHICAL DETECTION OF N-METHYL-PYRIDINE IN URINE OF RABBIT-ORGANISMS DOSED WITH PYRIDINEYOSHIO OKUDA1959 年 46 巻 1 号 p. 73-78
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリー1. This report is a study on the qualitative and quantitative micro-detection of N-methylpyridine in urine, which was obtained from rabbit-organisms administrated with pyridine, the detection was done by paper chromatography and ultraviolet absorption with spectrophotometer.
2. The N-methylpyridine (Methylpyridyl-ammoniumhydroxide) can be qualitatively micro-detected by means of the paper chromatograms colored with PABA-CNBr treatment, and the spot is pink-red, and giving Rf values 0.4-0.5, run with solvent n-butanol: acetic acid: water=4:1:2 or 4:1:5 (v/v) in mixture.
3. In the rabbit, N-methylpyridine has been excreted in urine during 5 days after pyridine was administrated, either in the case of injection, or per os.
4. The ultra-violet absorption spectra of acetone eluted N-methylpyridine in rabbit urine show the remarkable absorption maximum at 329 mμ in wave length (Fig. 2).
5. The quantitative estimation of N-methylpyridine in urine of rabbit-organisms dosed with 0.5g. pyridine was due to the paper chromatograms and the absorbance of the ultra-violet absorption by a Beckman's spectrophotometer, were as follows:抄録全体を表示PDF形式でダウンロード (539K) -
V. CONJUGATION OF SULFATE WITH PYRIDOXINE AND PYRIDOXAL IN RAT LIVERTOKURO SATO, MIEKO YAMADA, TAEKO SUZUKI, TOMITARO FUKUYAMA, HARUHISA Y ...1959 年 46 巻 1 号 p. 79-81
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーA study has been made of the biological sulfate conjugation with pyridoxine, pyridoxal and pyridoxamine. Pyridoxine and pyridoxal have been shown to be readily conjugated with sulfate in the presence of rat liver slices as well as homogenate.
The position of the conjugation was concluded to be in the phenolic hydroxyl group.抄録全体を表示PDF形式でダウンロード (136K) -
I. PAPER-CHROMATOGRAPHIC SEPARATION OF ACID-SOLUBLE PHOPHORUS COMPOUNDS INCORPORATING PHARUHISA YOSHIKAWA, MAKOTO NAKAO, KANJI MIYAMOTO, ISAMU YANAGISAWA1959 年 46 巻 1 号 p. 83-89
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーAcid-soluble phosphorus compounds of human erythrocytes were analysed with the aid of paper chromatography and P32. After 30 minute incubation of erythrocytes with P32 at 37° at least 15 radioactive compounds were obtained, including ATP, ADP, AMP, G-6-P, F-1, 6-DP, 2, 3-diphosphoglyceric acid, and 3-phosphoglyceric acid as identified. The presence of ribose-5-phosphate was indicated. An adenine nucleotide with two stable and two labile phosphate groups was also found.抄録全体を表示PDF形式でダウンロード (316K) -
KOZO NARITA, SHIRO AKABORI1959 年 46 巻 1 号 p. 91-92
発行日: 1959/01/25
公開日: 2008/11/18
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AKIRA TSUGITA, MASAYORI INOUE, SHIRO AKABORI1959 年 46 巻 1 号 p. 93-95
発行日: 1959/01/25
公開日: 2008/11/18
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TOKUJI KIMURA1959 年 46 巻 1 号 p. 97-99
発行日: 1959/01/25
公開日: 2008/11/18
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SHUNJI MIZUHARA, KYUICHIRO OKUDA, KENGO KURAHASHI, YOSHIYUKI IWADO1959 年 46 巻 1 号 p. 101-102
発行日: 1959/01/25
公開日: 2008/11/18
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SETSURO EBASHI, YASUO TOYOKURA, HIRONAO MOMOI, HIDEO SUGITA1959 年 46 巻 1 号 p. 103-104
発行日: 1959/01/25
公開日: 2008/11/18
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MAKOTO ISHIMOTO1959 年 46 巻 1 号 p. 105-106
発行日: 1959/01/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (98K)
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