巻号一覧
48 巻, 5 号
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TOMOMI SAKURADA1960 年 48 巻 5 号 p. 623-632
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. An azotometric method for the determination of CoA was established on the basis of the enzymic acetylation of INAH by the function of CoA and specificity of INAH in this azotometry.
2. The characteristics of this method are (i) absence of INAH consumption in the reaction of this system and (ii) absence of substances, other than INAH and its derivatives, which might generate nitrogen gas in this azotometry.抄録全体を表示PDF形式でダウンロード (532K) -
III. PHOTOOXIDATION OF CYTOCHROME C IN THE PRESENCE OF METHYLENE BLUEMISAKO NAKATANI1960 年 48 巻 5 号 p. 633-639
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. Cytochrome c was photooxidized in the presence of methylene blue. Its enzymatic activity and histidine content were decreased during the reaction.
2. The complete inactivation was brought about by a loss of 43 per cent of total histidine which corresponded to 1.3 moles per mole of cytochrome c.
The kinetic analysis of the reaction revealed the presence of two kinds, of histidine residues in terms of their susceptibilities to the photooxidation: “slow” destructable and “fast” destructable groups. Also it was suggested that only the “fast” destructable histidine is required for the activity of cytochrome c.
3. The absorption spectrum was modified considerably by photooxidation, and the decreases in absorbancy at 500 to 600mμ and 404mμ were particularly significant.
The author wishes to thank Prof. K. Okunuki and Dr. I. Sekuzu, Osaka Univ., for kindly supplying ‘crystalline cytochrome c’, and to express her appreciation for the invaluable advice and criticism of Prof. Y. Oshima and Prof. M. Funatsu, Kyushu Univ., during the course of this work.抄録全体を表示PDF形式でダウンロード (441K) -
IV. PHOTOOXIDATION OF PEROXIDASE IN THE PRESENCE OF METHYLENE BLUEMISAKO NAKATANI1960 年 48 巻 5 号 p. 640-644
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. Crystalline Japanese radish peroxidase was photooxidized in the presence of methylene blue. The enzymatic activity remained without a detectable loss when 85 per cent of the total histidine residues in the molecule was lost.
2. By photooxidation of the peroxidase, only a slight decrease in the absorbancy in the visible region was observed.
The authour wishes to thank Dr. Y. Morita, Kyoto University, for kindly supplying crystalline japanese radish peroxidase, and to express her appreciation for the invaluable advice and criticism of Prof. Y. Oshima and Prof. M. Funatsu, Kyushu University, during the course of this work.抄録全体を表示PDF形式でダウンロード (259K) -
III. THE IMMUNOLOGICAL ACTIVITIES OF INSULIN DERIVATIVESMASAYASU KITAGAWA, KAORU ONOUE, MOTOAKI ANAI, YUICHI YAMAMURA1960 年 48 巻 5 号 p. 645-650
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. Insulin derivatives were tested immunologically, using the absorption test and skin reaction.
2. Oxidized-, dinitrophenylated- and largely acylated-insulin lost not only the hormonal activity but the immunological activity.
3. Esterified-, acetylated-, iodinated- and phenylthiocarbamyl-insulin lost the hormonal activity but were still immunologically active.
The authors are very grateful to Dr. Y. Nojima, the Kodama Shoji Co., Dr. T. Sawada, 3rd Department of Internal Medicine, Faculty of Medicine, Kyushu University, Dr. T. Shibata, Taiyo Fishery Co., Ltd. and Dr. N. Okuyama, Department of Chemistry, Faculty of Science, Tokyo prefectural University, for gifts of insulin samples. We are also indebted to Dr. M. Yamaguchi, Research Laboratory of the National Sanatorium, Toneyama Hospital for preparation of heat killed tubercle bacilli in the series of these studies.抄録全体を表示PDF形式でダウンロード (369K) -
IV. ACTION OF α-CHYMOTRYPSIN ON ACYL-L-TYROSYLGLYCINES AND ACYL-L-TYROSYLGLYCYLGLYCINESTAKESHI YAMASHITA1960 年 48 巻 5 号 p. 651-661
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. A number of acyl-L-tyrosylglycines and acyl-L-tyrosylglyeylglyeines were synthesized and tested as substrates for α-chymotrypsin.
2. Using AcGly-L-TyrGlyGly and AcGly-L-TyrAm as substrates, the pH optimum of chymotryptic hydrolysis in phosphate buffer was found to be near 7.8.
3. When proteolytic coefficient (C) at an initial substrate concentration of 0.01M was used as a measure for susceptibility of a substrate to hydrolysis by α-chmotrypsin, the effect of acyl groups on the susceptibility was in a decreasing order of Bz-, CbzGly-, AcGly-, Cbz-, Form-, BzGly- and Ac-, and that for other groups which combined with carboxyl groups of tyrosine residues was in a decreasing order of -Am, -GlyGly, and -Gly.
4. The values of Km, k3 and Cmax. were obtained for several acetyl substances.
5. The inhibitory activities of structural analogues of several substrates for α-chymotrypsin were estimated against AcGly-L-TyrAm.
The author is grateful to Prof. S. Shibuya and Assoc. Prof. Izumiya for their guidance and encouragement, to Dr. M. Winitz for his interest in this study and to Mr. W. Hlmel for reading the manuscript.抄録全体を表示PDF形式でダウンロード (659K) -
MICHIO TSUKAMURA1960 年 48 巻 5 号 p. 662-671
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーPicric acid can be reduced enzymatically into picramic acid. It is conceivable that picric acid accepts hydrogen through flavotprotein.
Yellow picric acid can be converted into red picramic acid by whole cells and cell-free preparations under aerobic conditions. Picric acid appears to be utilizable as a redox dye.
The author wishes to express his appreciation to Dr. R. Katsunuma, director of the Obuso National Sanatorium, Prof. S. Hibino, Nagoya University, and Prof. N. Katsunuma, Osaka University, for their kind encouragement and advice. The author is also indebted to Mr. S. Mizuno for his technical assistance.抄録全体を表示PDF形式でダウンロード (532K) -
IV. DESTRUCTION OF ADENINE NUCLEOTIDES IN STORED BLOODMAKOTO NAKAO, MASAMITI TATIBANA, HARUHISA YOSHIKAWA1960 年 48 巻 5 号 p. 672-684
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーChanges in the concentrations of phosphorus compounds in red blood cells during storage in acid-citrate-dextrose medium and in the same medium supplemented with inosine at 4° were persued by column chromatography with the use of P32 as the indicator. The most prominent change was decrease of 2, 3-diphosphoglyceric acid and concurrent accumulation of inorganic orthophosphate. Adenosine triphosphate content declined continually during the storage due to retrogression of re-phosphorylation process and at the end of 8 weeks the total amount of adenine nucleotide became extremely low. On the other hand, inosine monophosphate was increased considerably, and hypoxanthine accumulated in an amount which was correspondent to the decrease of the total of nucleosides including inosine monophosphate.
After incubation of the stored blood with added inosine, an appreciable rise of sugar phosphates and 2, 3-diphosphoglycerate was observed, while the total amount of adenine nucleotides was slightly decreased though adenosine triphosphate was increased markedly. Inosine monophosphate was also increased by incubation with added inosine, and its considerably high specific radioactivity suggests that inosine monosphosphate may be formed not only from adenosine monophosphate by deamination but also synthetized newly from hypoxanthine.
In conclusion, ATP in erythrocyte undergoes gradual destruction to hypoxanthine, the enzyme involved in the process being adenosine triphosphatase, adenylate kinase, adenylic deaminase, 5'-nucleotidase, adenosine deaminase and nucleoside phosphorylase. Supplementation of inosine alone is not sufficient to restore adenosine triphosphate level in the long-stored erythrocytes.抄録全体を表示PDF形式でダウンロード (901K) -
V. INCORPORATION OF P32 DURING VERY SHORT TIME INTERVALSMASAMITI TATIBANA, KANJI MIYAMOTO, TAKASHI ODAKA, MAKOTO NAKAO1960 年 48 巻 5 号 p. 685-690
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーAs an attempt to elucidate the mechanism of phosphate uptake by human erythrocyte, P32 incorporation into acid-soluble organic phosphates during very short time period of incubation with added P32 orthophosphate was pursued by the use of ion exchange resin chromatography.
The P32 incorporation into 2, 3-diphosphoglycerate was found to be a rather sluggish one. In an incubation time up to 90 seconds no detectable labeling of the ester was obtained. Even after 30 minutes of incubation the specific activity of 2, 3-diphosphoglyceric acid was several times as small as that of ATP.
ATP and ADP became labeled to a detectable extent as early as 10 seconds after the addition of P32, when neither sugar phosphates nor phosphoglycerate took up practically no P32. The incorporation of P32 into ATP increases with time linearly with a very short lag. This suggests that there is an intermeniary step between inorganic orthophosphate in plasma and ATP in the cell. In addition to ATP, and ADP, several unidentified compounds with very early incorporation of P32 were found. However, their biological significance is ambiguous because they were present some times even in zero-time controls.
A very rapid isotopic equilibration among the labile phosphate groups of ATP and ADP was demonstrated.抄録全体を表示PDF形式でダウンロード (379K) -
III. DIGESTION OF CELLULOSEPOLYSULFATENORIKO TAKAHASHI1960 年 48 巻 5 号 p. 691-696
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. Cellulosepolysulfate was digested with cellulosepolysulfatase of the liver of Charonia lampas free from polysaccharases. Thus S-poor cellulose-sulfate was obtained.
2. After further digestion of the S-poor cellulosesulfate with polysaccharases, glucose 6-monosulfate and glucose were obtained,
3. In the digestion of cellulosepolysulfate by the crude liver extract, when the reaction was carried out in the cellophane bag dialyzing against acetate buffer, glucose 2- and/or 3-monosulfate and a small amount of glucose trisulfate were obtained as the intermediates.
The author thanks Prof. F. Egami for his interest and encouragement. A part of the expense of this study was defrayed by a grant from the Ministry of Eduction and from Seikagaku-Kenkyusho Ltd. to Prof. Egami, to which her thanks are due. Some of the experiments were carried out in the Marine Biological Institute of Nagoya University.抄録全体を表示PDF形式でダウンロード (405K) -
II. DIAZOTIZATIONYOSHIMI OKADA1960 年 48 巻 5 号 p. 697-705
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. Tuberculin active protein, hC, was diazotized with several aromatic diazonium compounds. p-APA-hC, which was prepared with p-aminophenol, and o-APA-hC, prepared with o-aminophenol, were as potent as unmodified hC. SAA-hC, prepared with sulfanilic acid, and AAA-hC, prepared with p-aminoacetophenone, were less potent than hC.
2. Amino acid analysis, particularly about aromatic and basic amino acids, was performed upon hC and diazo-hC's.
3. Mole ratios of several amino acids contents of hC are similar to those of crystalline tuberculin active peptide.
The author wishes to thank Prof. S. Akabori, Osaka University, for his conti-nuous interest and advice during the course of this investigation, and to thank Prof. Y. Yamamura, Kyushu University, for his encouragement and guidance. The author also wishes to thank Dr. K. Ogura, Toneyama Hospital, for his helpful advice, especially on the skin test technique.抄録全体を表示PDF形式でダウンロード (576K) -
VII. ISOLATION OF VENOM CHOLINESTERASE BY ZONE ELECTROPHORESISCHEN-CHUNG YANG, WEN-CHING CHIU, KUANG-CHING KAO1960 年 48 巻 5 号 p. 706-713
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーCholinesterase activity in the Formosan cobra and Banded krait venoms has been determined by colorimetric method of Hestrin. The results obtained were in good agreement with that of electrometric titration method. The cholinesterase activity in both Formosan cobra and Banded krait venoms were separated from venom toxicity by zone electrophoresis as an electrophoretically single fraction and the specific activity were increased 10.7 and 26.4 folds respectively. The results proved definitely that there is no relationship between the cholinesterase activity and the venom toxicity.
The authors are gratefully indebted to Prof. R. Hirohata, chief of the Laboratory of Protein Chemistry, Yamaguchi Medical School, Ube, for his continued interest and encouragement during the course of this study.抄録全体を表示PDF形式でダウンロード (395K) -
VIII. ELECTROPHORETIC STUDIES OF BANDED KRAIT (Bungarus Multicinctus) VENOM AND THE RELATION OF TOXICITY WITH ENZYME ACTIVITIESCHEN-CHUNG YANG, KUANG-CHING KAO, WEN-CHING CHIU1960 年 48 巻 5 号 p. 714-722
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. Banded krait (Bungarus multicinctus) venom was fractionated by zone electrophoresis into three fractions : one main protein fraction (2nd) with two minor (1 st and 3rd) protein fractions on both sides of it.
2. Although some toxic principles were detected in the 2nd protein fraction, the majority of the toxicity, representing 74.3 per cent of the recovered toxicity, was concentrated in the 1 st protein fraction.
3. All of the enzymes studied: phosphomonoesterase, phosphatidase A, cholinesterase, L-amino acid oxidase, and hyaluronidase activities were separated from the venom toxicity very distinctly, and were revealed as an electrophoretically single fraction just coinciding with the 3rd protein fraction.
4. Phosphodiesterase, 5'-nucleotidase, ATPase, DPN-pyrophosphatase, and proteinase activities in Banded krait venom were so feeble, hence they would not be a cause of venom toxicity.
5. It was definitely proved that the venom toxicity has nothing to do with any of enzyme activities studied, confirming our previous work.
The authors are gratefully indebted to Prof. R. Hirohata, chief of the Laboratory of Protein Chemistry, Yamaguchi Medical School, Ube, for his continued interest and encouragement during the course of this study.抄録全体を表示PDF形式でダウンロード (457K) -
HARUHIKO NODA, KOSÇAK MARUYAMA1960 年 48 巻 5 号 p. 723-732
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーA fibrous protein was extracted from the pellet formed by centrifuging the solution of myosin B in the presence of ATP. This protein had properties which were to be expected for the f-actin component separated from myosin B by the action of ATP. The length of the molecule estimated from the measurement of flow birefringence was about 1 to 3 micra which agreed well with that for myosin B. The amino acid composition of this protein was similar to actin obtained directly from muscle by the method of Straub.
When the f-actin from myosin B was combined with myosin A, reconstituted actomyosin was formed. The properties of the reconstituted actomyosin and its reaction with ATP were quite similar to those for myosin B from the viewpoints of flow birefringence, light scattering and superprecipitation.抄録全体を表示PDF形式でダウンロード (573K) -
IV. A PYRIDOXINE DEHYDROGENASE FROM BAKER'S YEASTYOSHIMASA MORINO, YUKIYA SAKAMOTO1960 年 48 巻 5 号 p. 733-744
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリー1. Pyridoxine dehydrogenase from baker's yeast was purified about 80-fold over crude extracts.
2. The enzyme required TPN ; DPN was not effective.
3. The reaction seems to favor the reduction of pyridoxal.
4. The optimum pH for this enzyme was pH 9.3.
5. The enzymatic activity was stimulated by Co++ and Mg++
6. The enzyme activity was inhibited by p-CMB. The inhibition by p-CMB was prevented by preincubation of the apoenzyme with TPN and reversed by the addition of reduced glutathione.
7. This enzyme seems to be concerned with the oxido-reduction reaction between the hydroxymethyl group and the formyl group at position 4 of pyridine ring.
8. The distribution of pyridoxine dehydrogenase was examined.
9. A possible action mechanism for B6-antagonists such as 5-deoxypyridoxine and 5-deoxypyridoxal is discussed.抄録全体を表示PDF形式でダウンロード (646K) -
II. ACTIVATION BY COBALTOUS ION AND REACTION MECHANISM OF TAKA-ACYLASEMASAYORI INOUYE, IKUNOSHIN KATO1960 年 48 巻 5 号 p. 745-757
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーThe activation of Taka-acylase by Co++ was invesigated kinetically. A scheme for the acylase reaction is proposed and it is concluded that Co++ mediates in the binding of the substrate to the enzyme and that with aromatic substrates such as ace tyl-L-phenylalanine, the enzyme-metal-substrate complex is stabilized by the interaction of the benzene ring of the substrate with a site of the enzyme. This is in contrast to the reaction with aliphatic substrates such as chloroacetyl-L-valine.
Inhibition by carboxylic acids and p-choloromercuribenzoate was also investigated kinetically.
A model of the active area of Taka-acylase is proposed and discussed.
The authors wish to express their deep gratitude to Dr. S. Akaboi, Prof. of Institute for Protein Research, for his kind guidance and Dr. Y. Ogura, Prof. of the University of Tokyo, for his valuable discussion throughout this investigation and also to Sankyo Co., Ltd. for kindly supplying “Taka-diastase Sankyo”.抄録全体を表示PDF形式でダウンロード (607K) -
ARASUKE NISHI1960 年 48 巻 5 号 p. 758-767
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーEnzyme activities involved in phosphorus metabolism in germinating spores of Aspergillus niger were investigated. A cell-free extract of the spores showed hydrolyzing activities on pyrophosphate, β-glycerophosphate, ATP, and polyphosphate. The spore extract also can transfer phosphorus from polyphosphate to ADP, producing ATP. The activities of these enzymes, except phosphomonoesterase and apyrase increased markedly during the course of germination, especially in an early period.
The author wishes to thank to Prof. T. Yanagita for his valuable advice in this work.抄録全体を表示PDF形式でダウンロード (1137K) -
JUNZO KATO1960 年 48 巻 5 号 p. 768-771
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (182K) -
TADASHI ASAHI1960 年 48 巻 5 号 p. 772-773
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (137K) -
IWAO SUZUKA, SHIGEAKI TANAKA, KENSUKE SHIMURA1960 年 48 巻 5 号 p. 774-776
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (155K) -
SADAKO SUGAI, FUJIO EGAMI1960 年 48 巻 5 号 p. 777-779
発行日: 1960/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (126K)
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