巻号一覧
50 巻, 1 号
選択された号の論文の16件中1～16を表示しています
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III. Oxidation and ReductionYOSHIMI OKADA1961 年 50 巻 1 号 p. 1-5
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリー1. Tuberculin active protein, hC, was treated with cysteine and thioglycollic acid but the tuberculin activity was not affected.
2. The hC was oxidized with performic acid. Tuberculin activity remained unchang-ed even after all of the cystine and/or cysteine
TABLE II Effect of Reagents on hC and Tuberculin Activity
residues were oxidized to cysteic acid.
3. When hC was photooxidized by visible light in the presence of methylene blue, inactivation of the tuberculin activity of hC took place along with the decomposi-tion of all of the histidine residues in the molecule.
4. It is probable that histidine residues are essential, but cystine or cysteine residues are not essential for the activity.
The author wished to thank Prof. Akabori, Osaka University, for his continuous interest and advice during the course of this investigation, and to thank Prof. Yamamura, Kyushu University, for his encouragement and guidance.抄録全体を表示PDF形式でダウンロード (381K) -
KAZUO SATAKE, MASARU TANAKA, HARUO SHINO1961 年 50 巻 1 号 p. 6-11
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリー1. TNP-Amino acid could be ammo-nolyzed to picramide and the corresponding amino acid with an excellent yield, under the action of ammonia at 110°C for few minutes.
2. Under these conditions, not only TNP-group in ω-amino acid, but also in ω-amino acid or in peptide was similary de-TNP-ated without any undesirable side-reactions, such as hydrolysis or other decompositions.
3. The only exceptions were TNP-glycine and -glycyl peptide, the ammonolysis of which was accompanied with appreciable amount of unidentified side-reaction (s).
4. These results were discussed mainly in connection with protein chemistry.抄録全体を表示PDF形式でダウンロード (378K) -
XXXVI. Isolation of a New Bile Sterol, 3α, 7α, 12α, 26-Tetrahydroxy-Δ23-bishomocholene, from Bull Frog BileTARO KAZUNO, TAKAKO MASUI, TAKAHIKO HOSHITA1961 年 50 巻 1 号 p. 12-19
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリー1. Two new and two already known bile sterols were isolated from bull frog bile by use of reversed phase partition chromatogra-phy.
2. It was verified that chemical consti-tution of one of the new bile sterols (m. p. 198°C) corresponded to 3α, 7α, 12α, 26-tetra-hydroxy-Δ23-bishomocholene.
3. 3α, 7α, 12α, 25-Tetrahydroxyhomocho-lane, 3α, 7α, 12α 26-tetrahydroxybishomocho-lane and 3α, 7α, 12α, 27-tetrahydroxycopro-stanc were synthesized.
The authors wish to thank Prof. S. Bergström of the Karolinska Institute who has kindly given Hostalene.抄録全体を表示PDF形式でダウンロード (445K) -
XXXIX. Metabolism of Lithocholic AcidKYUICHIRO OKUDA, TARO KAZUNO1961 年 50 巻 1 号 p. 20-23
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリー1. Lithocholic acid was incubated res-pectively with liver homogenates of rat and guinea pig.
2. Lithocholic acid was converted mainly to 3α, 6β-dihydroxycholanic acid and partly to chenodeoxycholic acid by rat liver homo-genate.
3. Guinea pig liver homogenate meta-bolized lithocholic acid mainly to chenode-oxycholic acid and partly to an unidentified dihydroxycholanic acid different from 3α, 6β-dihydroxycholanic acid.
The authors wish to thank Mr.-T. Hoshita for synthesis of 3α, 6β-dihydroxycholanic acid.抄録全体を表示PDF形式でダウンロード (255K) -
III. Terminal Amino Acids and Sugars of Porcine CaeruloplasminTOSHIO KAYA, SHIGEMASA OSAKI, TETSUO SATO1961 年 50 巻 1 号 p. 24-28
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーThe primary structure of porcine caerulo-plasmin was studied by paper chromato-graphy, paper electrophoresis and combination method of paper chromatography and electro-phoresis. The molecule is composed of common amino acids as well as three kinds of sugars. Both amino-terminal and carboxy-terminal amino acids were found to be phenylalanine. A sugar containing oligopep-tide was isolated after hydrolysis with Pro-nase-P. The peptide is made up of four amino acids : threonine, alanine, lysine and histidine. It was suggested that the sugar is bound to the ε-amino group of lysine.
The authors wish to express their deep gratitude to Prof. S. Hattori, University of Tokyo and Prof. T. Shibata and Dr. Nagahisa, Tokyo Institute of Technology, for their kind guidance throughout this work. The authors are also thankful to Mr. Kikuchi for his help in this investigation.抄録全体を表示PDF形式でダウンロード (312K) -
IV. The Effect of Borate Ion on the Oxidase Activity of Porcine CaeruloplasminSHIGEMASA OSAKI1961 年 50 巻 1 号 p. 29-33
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーThe oxidase activity of crystalline porcine caeruloplasmin was studied spectroscopically with p-phenylenediamine as the substrate. Borate ion inhibited the oxidase activity and the inhibition was found to be of reversible nature. The functional relationship obtained between borate concentration and the degree of inhibition indicated that the inhibition is the non-competitive type and occurs by combination of a borate ion with a sugar contained in the caeruloplasmin molecule. The structure of the active center near the copper atom of the molecule is discussed, refering the previous results described in this series of reports.
The author wishes to express his deep gratitude to Prof. S. Hattori, University of Tokyo, and Prof. T. Sato, Prof. K. Shibata and Dr. Nagahisa, Tokyo Institute of Technology, for their valuable advice and interest throughout this investigation. He is also thankful to Dr. Kanazawa, Mr. Kaya and Mr. Kikuclii for their help in carrying out this study.抄録全体を表示PDF形式でダウンロード (321K) -
VI. Metabolism of the Intermediates of the TCA Cycle and of the Glyoxylate Bypass in Brevibacterium flavum No. 2247ISAMU SHIIO, SHIN-ICHIRO ÔTSUKA, MASAHIRO TAKAHASHI1961 年 50 巻 1 号 p. 34-40
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリー1. When succinate was incubated aero-bically with the resting cells of Brevibacterium flavum No. 2247, α-ketoglutarate, or glutamate in the presence of ammonium salts, was accumulated. The addition of semicarbazide, of arsenite, and of monofluoroacetate caused the accumulation of oxaloacetate, of pyruvate, , and of citrate, respectively, instead of α-ketoglutarate. Similar results were obtained with the oxidative metabolism of L-malate, which was found to be formed from fumarate. From the inhibition experiments, following metabolic sequence of succinate was suggest-ed: succinate/fumarate/malate/oxaloacetate. pyruvate/citrate/α-ketoglutarate/glutamate.
2. When glyoxylate was incubated aero-bically with the resting cells, 2 moles of CO2 per mole of glyoxylate was obtained, indica-ting the complex oxidation. The reaction was accelerated by the addition of acetate. The anaerobic metabolism of glyoxylate wass observed only in the presence of succinate, with the formation of citrate.
The authors are indebted to Dr. H. Oeda, Mr. S. Motozaki, and Mr. T. Tsunoda of our laboratory for the helpful advices and encourage-ment.抄録全体を表示PDF形式でダウンロード (416K) -
II. Fractionation of the InhibitorTADAKAZ OHOKA1961 年 50 巻 1 号 p. 41-45
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリー1. A catalase inhibitor was isolated from silkworm blood plasma in a partially purified form, its specific activity being raised to about 500-fold as compared with the starting material.
2. The inhibiting action of the inhibitor was found to be intensified by preincubating the enzyme in the presence of nitrate, nitrite or chloride.
3. The increase in catalatic activity, ob-served on dilution of silkworm blood plasma, was explained by this effect of the anions present in the blood plasma.
The author wishes to express his thanks to Prof. A. Takamiya of the Tokyo University for valuable advices on this work.抄録全体を表示PDF形式でダウンロード (347K) -
I. The Phenol-hypochlorite Method for the Estimation of Amides and Related Compounds in Uptake StudiesA. DOMNAS1961 年 50 巻 1 号 p. 46-51
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーA new method has been described for the colorimetric estimation of amide and amide-like compounds, based on the phenol-hypo-chlorite reaction. Various factors such as effect of pH, color stability, order of reagent addition, Beer's law relationship and speci-ficity of reaction have been investigated. Water solutions, of allantoin, the main test compound can be determined in the range of 10-50μg. with an estimated precision of ±5%. Procedures are described for using the phenol-hypochlorite method in uptake studies. Tests in biological systems show that glucose, citrate and unidentified reducing substances are interfering factors.
The author wishes to thank Prof. L. Massart in whose laboratories this work was completed, and Dr. J. DeLey for his kindly criticism and advice. The author is indebted to the Arthritis and Rheuma-tism Foundation, New York, for financial assistance which permitted this investigation.抄録全体を表示PDF形式でダウンロード (380K) -
MICHIKO ITAHASHI1961 年 50 巻 1 号 p. 52-61
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーThe presence of choline sulfate in a high-er plant and the behavior of the exogenously supplied ester in the mouse were examined. As for the plant studied, the compound is neither formed in the tissue nor utilized as sulfur source. Choline sulfate injected into mouse bodies was very rapidly excreted into urine without changing its original form. The obtained results strongly suggest that this ester may be completely indifferent material both for the higher plant and for the animal.
The distribution of choline sulfate among 104 strains belonging to Phycomycetes, As-comycetes, Fungi Imperfecti; Basidiomycetes and Bacteria has been summarized. The results so far obtained suggest that the pre-sence of choline sulfate is restricted to the fungi belonging to the subclass Euascomycetes and other several fungi taxonomically closely relating to the subclass.
The author wishes to express her sincere appre-ciations to Prof. F. Egami (University of Tokyo), Prof. T. Miwa (Tokyo University of Education) and Prof. T. Mori (Nagoya University) for their valuable guidance throughout this work. This research has been supported in part by the Grant in Aid for Sci-entific Research of the Ministry of Education.抄録全体を表示PDF形式でダウンロード (622K) -
KENTARO TANAKA1961 年 50 巻 1 号 p. 62-69
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリー1. The RNase of Streptomyces erythreus was purified 1, 700-fold by the combination of acrinol precipitation and by DEAE-cellulose column chromatography.
2. The enzyme preparation was found to be thermostable.
3. By digestion of yeast RNA with the enzyme preparation, guanosine-2', 3'-cyclic phosphate was formed at an early stage, 3'-guanylic acid at a later stage, and other mononucleotide was scarcely detected.
4. Guanosine-2', 3'-cyclic phosphate was the only cyclic mononucleotide which was digested with this RNase preparation.
5. Even after the exhaustive digestion of yeast RNA with this preparation, resistant polynucleotides remained.
6. The terminal nucleotide of the resis-tant polynucleotides was exclusively guanylic acid.
7. Besides the main peak of RNase activity (Fraction A), a very small peak (Fraction B) was found in DEAE-cellulose chromatography and these two fractions had apparently the same specificity for yeast RNA and cyclic nucleotides.
The author wishes to thank Dr. T. Sakaki for his kind gift of rabbit appendix DNA. He is also indebted to Dr. E. Iwase for the gift of U. V. filter of Scientific Research Institute.抄録全体を表示PDF形式でダウンロード (1841K) -
HIDEMI HAYASHI1961 年 50 巻 1 号 p. 70-71
発行日: 1961年
公開日: 2008/11/18
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KAZUHIKO YAMADA, HIROMI YAMADA, YOSHIKI TAKESUE, SHOZO TANAKA1961 年 50 巻 1 号 p. 72-73
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (112K) -
MINORU KAWADA, KAZUO YAMADA, YASUO KAGAWA, YOSHITAKE MANO1961 年 50 巻 1 号 p. 74-76
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (159K) -
FUMIKO EBASHI1961 年 50 巻 1 号 p. 77-78
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (84K) -
Removal of Bound Nucleotide and Calcium of G-Actin by Treatment with Ethylenediaminetetraacetic AcidYUJI TONOMURA, JUNKO YOSHIMURA1961 年 50 巻 1 号 p. 79-80
発行日: 1961年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (133K)
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