巻号一覧
50 巻, 3 号
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Ueber die Freien Aminosaeuren in ZahnpulpaSEIICHI MOTOMURA1961 年 50 巻 3 号 p. 167-171
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーIn dem wãssrigen Extrakt von Rinderzahnpulpen wurden folgende freie Aminosäuren nachgewiesen: Lysin, Arginin, Histidin, Glutamin-, and Asparaginsäuren, Serin, Threonin, Prolin, Alanin, Valin, Phenylalanin, Isoleucin, α-und γ-Aminobuttersäuren.
Herrn Prof. Dr. M. Tomita spreche ich für seine freundliche Anleitung meinen verbindlichsten Dank aus.抄録全体を表示PDF形式でダウンロード (265K) -
SEIICHI MOTOMURA1961 年 50 巻 3 号 p. 172-177
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーIn dem Zahnhartgewebe von Rind wurden folgende freie Aminosäuren mit Glycerin extrahiert: Glutaminsäure, Aspara-ginsäure, Lysin, Arginin, Histidin, Serin, Threonin, Cystin, Prolin, Hydroxyprolin, Tyrosin, Valin, Phenylalanin, Leucin, β-Alanin and γ-Aminobuttersäure. Mit Wasser wurde weder Cystin noch Hydroxyprolin extrahiert.
Aus Zahnhartgewebe wurden zwei unbe-kannte Substanzen isoliert, welche ω-Amino-säuren zu sein scheinen. Die eine gab gelben Fleck durch Ninhydrin aber kannte mit γ-A.minocrotonsaure, die auch gelben Fleck aufzeigt, nicht identifiziert werden, während die andere nicht mit β-Amino-isobuttersaure identifiziert wurde.
Herrn Prof. Dr. M. Tomita spreche ich für seine freundliche Anleitung bei der Ausführung dieser Arbeit meinen verbindlichsten Dank aus.抄録全体を表示PDF形式でダウンロード (359K) -
SEIICHI MOTOMURA1961 年 50 巻 3 号 p. 178-181
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーIn dem Nervus mandibularis von Rind wur-den chromatographisch folgende freie Amino-säuren nachgewiesen: Lysin, Arginin, Histidin, Asparaginsäure, Glutaminsäure, Threonin, Glycin, Prolin, Leucin, Phenylalanin, Valin and Alanin, sowie zwei ω-Aminosäuren: β-Alanin and γ-Aminobuttersäure.
Herrn Prof. Dr. M. Tomita spreche ich hier-mit für seine freundliche Anleitung bei der Ausführung dieser Arbeit meinen verbindlichsten Dank aus.抄録全体を表示PDF形式でダウンロード (253K) -
HIROSHI ASAI1961 年 50 巻 3 号 p. 182-189
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリー1. For the purpose of investigating the polymerization mechanism, the electric bire-fringence of'rabbit tropomyosin was measured at various conditions of solutions by the use of the rectangular and reversing pulse method. Electric dipole moments and lengths of mono-mers and polymers of tropomyosin were determined by the analysis of transient elect-ric birefringence behaviors.
2. It was found that the aggregated tropomyosin molecules were completely de-polymerized to monomers by infinite dilution even under salt-free condition and that tropo-myosin monomer had a permanent dipole moment of about 390 Debye unit at neutral pH.
3. Theoretical analysis was successfully made on the relation between the birefrin-gence and the tropomyosin concentration under the assumption of the reversible linear polymerization. As a result, it was concluded that the polymerization was not only due to the head-to-tail association but also due to the head-to-head or antiparallel side-by-side association, by which the permanent dipole of polymer was canceled in part. Also, it was shown that the binding constant between two tropomyosin molecules was decreased with increasing concentration of salt or urea.
4. At temperature higher than 30°C, associated tropomyosins were abruptly de-polymerized and birefringence also decreased. At pH lower than 6.0 and at pH higher than 9.0, birefringence remarkably decreased.
The author expresses his thanks to Prof. F. Oo-sawa and Dr. T. Ooi for their helpful advice and discussion in this investigation.抄録全体を表示PDF形式でダウンロード (1283K) -
SIGEHIRO MORITA1961 年 50 巻 3 号 p. 190-196
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリー1. Using Rhodopseudomonas palustris as material, investigations were made of the aerobic metabolism, in light and darkness, of α-ketoglutaric, succinic, fumaric and malic acids.
2. The molar amounts of oxygen taken up for one mole of each substrate consumed were found to be as follows:
In light: α-ketoglutaric, 1.13; succinic, 0.73; malic, 0.45.
In dark: α-ketoglutaric, 1.51; scccinic, 1.45; malic, 0.92.
3. Experiments with uniformly-C14-la-beled fumarate and succinate showed that the distribution of their four carbons in cell material and in carbon dioxide evolved was modified by light-and-dark conditions as follows:
In light: 1.5 atoms per mole in CO2, 2.5 atoms per mole in cell material.
In dark: 2.5 atoms per mole in CO2, 1.5 atoms per mole in cell material.
4. Using 1, 4-C14-labeled succinate as sub-strate, it was revealed that the two carboxyl-C14 were distributed to cell material and carbon dioxide evolved in the following ratio:
In light: 1.5 atoms per mole in CO2, 0.5 atoms per mole in cell material.
In dark: 1.9 atoms per mole in CO2, 0.1 atom per mole in cell material.
5. In the light, and when potassium hydroxide was applied as carbon dioxide absorber, the efficiency of incorporation of substrate (succinate) carbon into cell material was the same as that in the presence of sufficient amounts of non-labeled carbon dioxide in the reaction mixture, indicating that in the presence of the carbon dioxide absorber, the respiratory C14O2 produced from the substrate is not assimilated photosynthe-tically.
6. Based on the present data and those obtained earlier with pyruvate, the following sequence of reactions was assumed for the metabolism of the organic acids in Rhodopseudomonas palustris.
It is a pleasure for the author to express his thanks to Prof. H. Tamiya for his continuous guidance and keen interest throughout this work. His thanks are also due to Prof. A. Takamiya for helpful advice and kind suggestions. The author is also indebted to Dr. H. Takahashi and Dr. S. Kat: oh for their help to prepare the radioactive compounds.抄録全体を表示PDF形式でダウンロード (460K) -
A New O-Acetyl Sugar Obtained from Leucomycin Minor ComponentsTETSUO WATANABE, TADASHIRO FUJII, KAZUO SATAKE1961 年 50 巻 3 号 p. 197-201
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (345K) -
KOHEI HAYASHI, TADAO KANOH, SABURO YAMAZOE1961 年 50 巻 3 号 p. 202-209
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーThe incorporation of P32-labelled ortho-phosphate into heart and femoral muscle phosphatides of rat has been investigated in vivo by three methods: column chromatography with silicic acid, paper chromatography on silicic acid-impregnated paper and two-dimen-sional paper chromatography of hydrolysates of phosphatides. From the results obtained some criticism of those methods was made.
1. The phosphatide content of heart muscle (500-750μg. of P per g. of wet tissue) was about twice as great as in femoral muscle (300-350μg. of P per g. tissue). The specific radioactivity of phosphatides in heart muscle, was about 7-8 times as high as that in femoral muscle at the second hour after the injection of P32. However, these muscles showed almost the same activity 16 hours after the injection.
2. Lecithin was the most abundant phosphatide, and phosphatidylethanolamine was the next in both muscles, and the sum of the two phosphatides amounted to more than 51 and 68 per cent of the total phos-phatides in heart and in femoral muscle respectively.
3. The radioactivity of P32 incorporated into lecithin was about 50 per cent of the radioactivity incorporated into the total phos-phatides in both types of muscle. However the specific radioactivity of lecithin was lower than that of the total phosphatides until 16 hours after P32 injection.
4. The specific radioactivity of inositol phosphatide was highest, paticularly in heart muscle. It was about 3 times as high as that of the total phosphatides.
5. The specific radioactivity of phos-phatidic acid was high only at the early stage after Par injection.
6. The specific radioactivity of phos-phatidylethanolamine was lowest, in spite of its relatively large content in the tissue.
7. A single spot separated on silicic acid impregnated paper did not consist of a single phosphatide, at least when an exactly measu-rable amount of phosphatides was applied. Therefore this technique by itself seemed to be insufficient for quantitative analysis of phosphatides. It was useful, however, in determining the composition of each fraction separated previously by column chromato-graphy with silicic acid.
The authors wish to express their gratitude to Miss M. Takayama for assistance in the perfor-mance of various analytical procedures.抄録全体を表示PDF形式でダウンロード (2382K) -
YOICHI KONDO1961 年 50 巻 3 号 p. 210-217
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーA thyroidal cell-tree system wnicn is ante to iodinate thyroglobulin effectively is report-ed.
When thyroglobulin was aerobically in-cubated at 37°C with pig thyroid particulate fraction (mitochondria-microsomes), iodide, glucose and glucose oxidase, iodine was in-corporated into thyroglobulin efficiently, and iodinated amino acids were formed. The particulate fraction, too, was iodinated, but the extent was less than in the case of thyro-globulin. The reaction appears to be specific since the liver particulates fraction was in-effective and serum or egg albumin could not be iodinated in the present system. Mg++ or Ca++ ions inhibited the reaction. Thyro-tropic hormone (TSH) was found to affect the reaction, but the effect depended upon the concentration of particulates in the in-cubation medium.
The author is indebted to Prof. N. Ui for his guidance and encouragement and to Dr. T. Hosoya for his criticism. Thanks are also due to Miss H. Tamura for her technical assistance. The expense of this study was defrayed in part by a grant from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (2026K) -
KEN HOTTA1961 年 50 巻 3 号 p. 218-229
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリー1. Two kinds of SH groups were defined chemically and enzymatically, i. e., difference in reactivity with the SH reagents and inter-action with substrates.
2. Role of divalent cations, especially as the chelaters of substrates have been discussed showing the evidence that the existence of interaction between the ring and groups and Mg++ or Ca++.
3. Rate-retarding steps of myosin NTP-ase among the consecutive enzymatic process are suggested. For ATP class, at neutral pH, rate-retarding step is desorption of ADP. For the other pH, AET treated myosin for ATP, and ITPase case, that is hydrolytic process.
4. Histidine group introduced as a possi-ble group for NTPase of myosin from the pH dependency of enzymatic action and contractility of muscle model.
5. Model for active site is proposed as a combination of two kinds of SH groups and histidine group. One of the SH groups and the histidine group form the hydrolytic site and one SH group makes the substrate Ifinding site.
6. Relation between ATPase and cont-raction mechanism of the muscle model were discussed briefly.
I would like to express my sincere thanks particu-larly to Prof. M. F. Morales for valuable sugges-tions and discussions. I also wish to thank Dr. Watanabe for helpful discussions, and Drs. Blum and Tonomura for communicating to me their unpublished data.
This research was supported in part by National Herrt Institute Research Grant H-3598, and was per-formed during tenure of a Fulbright scholarship.抄録全体を表示PDF形式でダウンロード (881K) -
KIICHIRO TANAKA1961 年 50 巻 3 号 p. 230-235
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーWhen capillin, a polyyne antifungal substance, was incubated with freshly harvested mycelium of Penicillium italicum, its carbonyl group was found to be specifically reduced with resultant formation of a cor-responding polyyne alcohol, capillinol. Such a reduction was also observed by using Piricularia oryzae as a test organism. Important is the fact that the reduction occurred stereo-specifically producing one of stereoisomers of capillinol.
In relation to the present finding, further investigations were carried out to examine the capacity of various microorganisms to reduce capillin. Remarkable reduction of capillin was observed in yeasts and fungi, whereas practically no reduction in bacteria so far tested. Thus the reduction of capillin was considered to be correlated with the antibiotic activity of this antibiotic.
The author wishes to express his deep gratitude to Prof. T. Yanagita of Chiba University and Prof. A. Takamiy a of University of Tokyo for their kind guidance and enthusiastic encouragement throughout this work. Thanks are also due to Prof. H. Umezawa of University of Tokyo for his valuable advice in the preparation of this paper, and to Mr. M. Matsui, director, and Dr. Y. Miura and Dr. I. Iwai of this Laboratory for their interest and encouragement. The author is indebted to Messrs. T. Once, O. Amakasu, and H. Higuchi, and to Misses C. Furukawa and H. Ohtsuka of this Laboratory, for elemental analysis and infrared spectrophotometry.抄録全体を表示PDF形式でダウンロード (409K) -
SETSURO EBASHI1961 年 50 巻 3 号 p. 236-244
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリー1. A minute amount of calcium is es-sential for actomyosin superprecipitation.
2. Relaxation activities of some chelat-ing agents have a definite correlation with their calcium binding activities.
3. ATP-dependent calcium binding activ-ity of vesicular relaxing factor has a capacity comparable to that of the chelating agents. This accounts for the relaxing activity of the factor.
4. The role of the vesscular relaxing factor, presumably identical with some part of the endoplasmic reticulum, in physiological process of muscular contraction was discussed. The possible role of calcium in the mechanism linking excitation and contraction was sug-gested.
The author wishes to express his sincere thanks to, Prof. F. Lipmann for his encouragement and advice. Thanks are also due to Prof. G. E. Palade for his suggestions and Mr. R. Traut for his kind assistance in preparing this manuscript and for his discussions.抄録全体を表示PDF形式でダウンロード (617K) -
ITARU TAKEBE1961 年 50 巻 3 号 p. 245-255
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーA strain of Pseudomonas which is able to grow on choline sulfate as its sole organic substrate was isolated from soil. This organism was shown to form adaptively an enzyme catalyzing hydrolysis of choline sulfate into choline and inorganic sulfate. Studies on the behavior of the partially purified enzyme preparation toward various sulfate and choline esters led to the conclusion that this enzyme is a sulfatase of a so far unknown type, and the name “choline sulfatase” was proposed. Choline sulfatase was shown to have a pH optimum of 8.3 and to be inhibited by sulfite but not by sulfate, phosphate, fluoride or cyanide. The role of this enzyme in choline sulfate metabolism in microorganisms is discussed.抄録全体を表示PDF形式でダウンロード (1406K) -
II. Amino Acid Composition and Terminal Sequences of Bonito InsulinAKIRA KOTAKI1961 年 50 巻 3 号 p. 256-263
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリー1. Bonito insulin-II contained two differ-ent polypeptide chains, glycyl (A) and alanyl (B), which could be resolved only after treat-ment with performic acid.
2. Electrophoretic and amino acid analy-ses suggested the presence of three disulfide linkages, two as interpeptide linkage and one as intra-peptide linkage in the A-chain.
3. Amino acid compositions of alanyl (bonito) and phenylalanyl (bovine) chains were fairly similar, although the sequences of ala-nyl chain was H-Ala-Ala-Asp (or AspNH2)-Glu (or GluNH2)-Pro-Lys-OH instead of H-Phe-Val-AspNH2-Thr-Pro-Lys-Ala-OH in the mammalian insulins.
4. Amino acid composition of the A-chain differed as much as 30 per cent (=6/21) from the corresponding bovine one although they had just the same N-and C-terminals.
The author wishes to express his thanks to Prof. K. Satake for his valuable guidance and encourage-ment during the cource of this work.抄録全体を表示PDF形式でダウンロード (552K) -
I. Comparison of Laccases Obtained from Rhus vernicifera and Rhus succedaneaTSUNEO OMURA1961 年 50 巻 3 号 p. 264-272
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリー1. Highly purified laccases were obtained by ion-exchange column chromatography from Japanese lacquer tree (Rhus vernicifera) and Indo-Chinese lacquer tree (Rhus succedanea). From comparative studies of the two enzymes, it was concluded that they represent different chemical entities.
2. The two laccases have different pH optima and Michaelis constants in the oxida-tion of hydroquinone, catechol, and L-ascorbic acid. R. succedanea laccase has a much higher specific activity and heat stability than R. vernicifera laccase.
3. Both enzymes are strongly blue in color and contain nearly equal amounts of copper. Calcium and traces of magnesium can also be detected in the two enzymes. Their absorption spectra are closely similar to each other, though the positions of absorption maxima are slightly different in the two.
4. Molecular weights of both laccases calculated from their sedimentation coeffici-ents, and partial specific volumes are nearly the same. The two enzymes both contain 5 or 6 atoms of copper per molecule.
The author wishes to express his hearty gratitude to the late Prof. A. Nishimura for his interest and encouragement during this investigation, and to Prof. Y. Ogura, University of Tokyo, for his valuable advices. Thanks are also due to Mr. S. Yamamoto, the Institute for Infectious Diseases, University of Tokyo, for sedimentation measurements, to Miss K. Watatsu, the Institute of Applied Microbiology, University of Tokyo, for the operation of Spinco model H electrophoresis apparatus, and to Misses F. Shimazaki and Y. Asahi for their co-operation in this work. The author also expresses his gratitude to Saito Co. Ltd. for the kind supply of lacquer latexes.抄録全体を表示PDF形式でダウンロード (1448K) -
TAKASHI KAWACHI, SETSURO FUJII, TOMOKAZU SUZUKI, YUICHI YAMAMURA1961 年 50 巻 3 号 p. 273-274
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (130K) -
AKIRA KOBATA, MAKOTO KIDA, SUZUOKI ZIRÔ1961 年 50 巻 3 号 p. 275-276
発行日: 1961/09/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (145K)
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