巻号一覧
52 巻, 6 号
選択された号の論文の16件中1～16を表示しています
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KOICHIRO SUMIZU1962 年 52 巻 6 号 p. 383-389
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリーCysteinesulfinic desulfinase which cleaves CSA to alanine and SO2 was identified in rat liver. The optimum pH of this enzyme was 6.25 (0.2M acetate buffer) at 37.5°C. The optimum temperature for the enzyme reaction was 37.5°C. Pyridoxal-5-phosphate was essential for the enzyme activity, calcium or magnesium ions increased the enzyme activity in the presence of pyridoxal-5-phosphate. The K, value was 2.2×10-3M (cysteinesulfinic acid), and the Ka. value was 2.5×10-4M (pyridoxal-5-phosphate).
The anther is indebted to Prof. O. Hayashi, Prof. T. Suzuki and prof. S. Tanaka for discussions on this work. The author wishes to express his gratitude Mr. H. Yamada for his continuing interest in this work.抄録全体を表示PDF形式でダウンロード (495K) -
I. Screening of Organisms and Investigation of Cultural ConditionsMASAHIKO KISUMI1962 年 52 巻 6 号 p. 390-399
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリーStudies on isoleucine fermentation using D-threonine as a precursor were carried out and cultural method which enables accumu-lation of a large amount of L-isoleucine was established.
1. A number of organisms were screened by testing the activity of accumulating isoleucine in the media containing D-threonine. As a result, the bacteria belonging to genus Serratia and Pseudomonas were found to be suitable strains for the isoleucine formation.
2. By using the most favorable strain, Serratia marcescens No. 1, various cultural con-ditions for the production of isoleucine were investigated, and the best condition so far obtained is as follows.
Serratia marcescens No. 1 was incubated in the medium containing 2% D-threonine, 10% glucose, 0.7% corn steeb liquor, 0.5% urea, 0.1% K2HPO4, 0.05% MgSO4•7H2O and 2 CaCO3 with a vigorous shaking (130c.p.m., 8cm. stroke) for 40 hours at 30°C, and isoleu cine over 8mg./ml. accumulated without the formation of other amino acids.
3. Isoleucine was isolated from the broth by the ordinary separation procedure employing ion exchange resin and the crystals obtained were identified as L-isoleucine.
4. Any accumulation of an appreciable amount of isoleucine was not observed in the respective media containing L-threonine, DL-allothreonine and other precursors of isoleucine as the substitute of D-threonine.
The author wishes to thank President S. Akabori, Prof. H. Kikkawa and Assistant Prof. Y. Izumi, Osaka University, for their interest and helpful advice throughout this work. I am also greatly indebted to to Mr. T. Hirabayashi, Representative Director of the Tanabe Seiyaku Co., Ltd., Dr. M. Fujisawa, Research Executive, Dr. N. Sugimoto, Director of Osaka Research Laboratory, Dr. I. Utsumi, and Dr. I. Chibata, and the staffs of the Biochemical Section of the Laboratory for continued encouragement and assistance.抄録全体を表示PDF形式でダウンロード (705K) -
II. On the Mechanism of Isoleucine Formation and Threonine DehydraseMASAHIKO KISUMI, YOSHITARO ASHIKAGA, JYOJI KATO, ICHIRO CHIBATA1962 年 52 巻 6 号 p. 400-408
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリーThe mechanism of isoleucine formation from D-threonine in isoleucine fermentation was studied with special reference to the rôle of D-threonine dehydrase.
1. Optimum condition for the formation of D-threonine dehydrase coincides with that for isoleucine fermentation.
2. D-Threonine dehydrase was inducibly formed by D-threonine.
3. DL-Allothreonine dehydrase activity was not found in the media containing any threonine isomer.
4. Although L-threonine dehydrase was markedly inhibited by lower concentration of L-isoleucine, D-threonine dehydrase activity was not inhibited by the presence of isoleucine at such a concentration as produced during the fermentation.
5. Presence of L-threonine resulted in the repression and inhibition of D-threonine dehydrase.
6. Although the difference in the iso-leucine forming activities from α-ketobutyric acid between the cells grown in the media containing L- and D-threonines was not so large, the latter activity was slightly better than the former.
From these facts, it was confirmed that D-threonine dehydrase plays an important rôle for this isoleucine fermentation using D-threonine, and that specific isoleucine accumulation observed only in the medium containing D-form among the threonine isomers is also explained by the difference of the threonine dehydrase activities.
The author wishes to thank President S. Akabori, Prof. H. Kikkawa and Assist. Prof. Y. Izumi, Osaka University, for their interest and helpful advice throughout this work. I am also greatly indebted to Mr. T. Hirabayashi, Representative Director of the Tanabe Seiyaku Co. Ltd., Dr. M. Fujisawa, Research Executive, Dr. N. Sugimoto, Director of Osaka Research Laboratory for continued encouragement and assistance.抄録全体を表示PDF形式でダウンロード (588K) -
TUNG-BIN LO1962 年 52 巻 6 号 p. 409-422
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリーThe polypeptide concentrates from the posterior-intermediate lobes of bovine and equine pituitary glands have been studied extensively by countercurrent distribution. Various active peptides, namely, β-MSH, vasopressin, α-MSH, and adrenocorticotropin, have been resolved by countercurrent distribution in two different system, 0.1%, acetic acid: n-butanol: pyridine (11:5:3), and 0.5% aqueous triehloroacetic acid: 2-butanol. In addition to the above-mentioned known peptide hormones, the isomers corresponding to α- and β-MSH have been isolated. These isomers showed poor malanocyte-stimulating activity compared with known α- and β-MSH. They were found to be methionine sulfoxide analogues of the latter. The presence of methionine sulfoxide instead of methionine in these analogues was demonstrated by amino acid analyses of alkaline hydrolysates and enzymatic digests, as well as from oxidation-reduction behavior correlated with biological activity.抄録全体を表示PDF形式でダウンロード (1065K) -
SHIRO OHNOKI, GINZABURO SUZUE, SHOZO TANAKA1962 年 52 巻 6 号 p. 423-427
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリー1. The formation of phosphorylated derivatives of mevalonic acid in the presence of adenosine triphosphate and Mn++ by enzymes extracted from Staphylococcus is described.
2. One of the products was purified by various chromatographic procedures and shown to be identical with 5-phosphomeva-lonic acid.
3. 5-Phosphomevalonic acid was shown to be converted to phytoene with twice the efficiency of the DL-mevalonic acid.抄録全体を表示PDF形式でダウンロード (392K) -
IV. Indole Hydroxylase and Aniline HydroxylaseTORU OTANI, KATSUKO AKAGI, YUKIYA SAKAMOTO1962 年 52 巻 6 号 p. 428-432
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリー1. Aniline hydroxylase had an optimum at pH 7.8, while that of indole hydroxylase was at pH 6.4.
2. Indole hydroxylase was less stable than aniline hydroxylase on storage.
3. In rabbit and hamster liver, indole hydroxylase was more active than aniline hydroxylase, while in guinea pig and mouse liver aniline hydroxylase was more active.
4. The degree of increase in enzyme activity after administration of methylcholanthrene differed for the two hydroxylases.
5. o-Phenanthroline strongly inhibited the hydroxylation of IBA while EDTA and KCN, which only inhibited the hydroxylation of IBA slightly, strongly inhibited that of aniline.
6. The activity of indole hydroxylase apparently decreased more than of aniline in regenerating liver.
7. It is concluded that indole hydroxylase and aniline hydroxylase are different enzymes. Indole hydroxylase is specific for indole compounds.抄録全体を表示PDF形式でダウンロード (286K) -
II. Tritiation of Deoxyribonucleic AcidsTORU TSUMITA, MICHIKO IWANAGA, TOMOYOSHI KOMAI1962 年 52 巻 6 号 p. 433-439
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリー1. Deoxyribonucleic acids of calf thymus and chick embryo were tritiated by the exposure to gaseous tritium. The tritiation did not affect at all the base composition but their ε(P) values increased in a limited extent (Table I).
2. The labelled DNAs were analyzed on specific radioactivities of base, deoxyribose and deoxyribonucleoside as well as residual protein.
3. Calf thymus DNA was hydrolyzed with deoxyribonuclease and a snake venom (‘Habu’) and components were isolated and purified. Among the bases, thymine was the most radioactive (7×106sc.p.m./μmole) as compared with guanine, adenine and cytosine (Table II).
4) Deoxyribose isolated from deoxy-guanosine and -adenosine had the same radio-activity, 5-6×lO4 c.p.m./μmole. At the level of deoxyribonucleoside, the specific radioactivity of thymidine was less than those of others (Table III) and, from the non-dialyzable fraction of the enzyme hydrolyzate, a highly active portion of thymine was found (Table IV).
5) Among amino acids of the residual protein, glycine and alanine contained higher radioactivities than other amino acids in both calf thymus and chick embryonal DNAs.
6) The susceptibility of tritiated DNA against hydrolyzing enzymes and the distribution of radioactivity of thymine were discussed.
The authors wish to thank Dr. A. Ohsaka for the supply of the snake venom (‘Habu’).抄録全体を表示PDF形式でダウンロード (534K) -
TOSHIHIKO UBUKA1962 年 52 巻 6 号 p. 440-443
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリーFor the identification of isovalthine and its related compounds, a combined method of high-voltage paper electrophoresis and paper chromatography is described.抄録全体を表示PDF形式でダウンロード (1821K) -
MASATERU SHIN, WATARU NAKAMURA1962 年 52 巻 6 号 p. 444-451
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリー1. It was shown that the IAA oxidase activity in wheat germ was solely attributed to the peroxidases isolated by the present authors. The IAA oxidase activity was divided into two activities, an “Acidic IAA Oxidase Activity” and a “Neutral IAA Oxidase Activity”.
2. The neutral IAA oxidase activity of peroxidase 566 had the pH optimum at 6.4 and was stimulated by 10-5M of manganese ion and inhibited by the larger amount of it. Catalase inhibited the activity.
3. The acidic IAA oxidase activity of peroxidase 556 had the pH optimum at 4.0 and was stimulated remarkably by 10-2M of manganese ion. Lower concentrations of manganese ion also stimulated the activity. Peroxidase 556 was inactive at neutral pH's.
The authors express their many thanks to Prof. Okunuki, K. for his guidance and encouragement during the work, to Prof. Hagihara, B. for analysis of oxygen consumption and his advice and to Dr. Ito, T. for his helpful discussion for the work.抄録全体を表示PDF形式でダウンロード (544K) -
TOSHIHARU NAGATSU, KUNIO YAGI1962 年 52 巻 6 号 p. 452-454
発行日: 1962/12/25
公開日: 2008/11/18
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TYUNOSIN UKITA, TADAO TERAO, MASACHIKA IRIE1962 年 52 巻 6 号 p. 455-457
発行日: 1962/12/25
公開日: 2008/11/18
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YOSHINARI ISHII, TAKASHI HASHIMOTO, MASAMITI TATIBANA, HARUHISA YOSHIK ...1962 年 52 巻 6 号 p. 458-460
発行日: 1962/12/25
公開日: 2008/11/18
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KIHACHIRO UEHARA, MASAO TAKEDA1962 年 52 巻 6 号 p. 461-463
発行日: 1962/12/25
公開日: 2008/11/18
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KAZUHIKO WAKABAYASHI, SACHIKO KAMEI, HIROSHI MURAKAMI, NORIO SHIMAZONO1962 年 52 巻 6 号 p. 464-465
発行日: 1962/12/25
公開日: 2008/11/18
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KUNIO KONNO, TSUNAO TETSUKA1962 年 52 巻 6 号 p. 466-467
発行日: 1962/12/25
公開日: 2008/11/18
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SEN-ITIROH HAKOMORI, TAIKO ISHIMODA, KIYOSHI NAKAMURA1962 年 52 巻 6 号 p. 468-469
発行日: 1962/12/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (950K)
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