巻号一覧
53 巻, 4 号
選択された号の論文の13件中1～13を表示しています
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I. Metabolic Conversion of Ricinoleic Acidy by a Certain Microorganism to 8-D-(+)-Hydroxy- Tetradesc-cis-5-enoic AcidSEIICHI OKUI, MITSURU UCHIYAMA, MICHINAO MIZUGAKI1963 年 53 巻 4 号 p. 265-270
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリーRicinoleic acid is metabolized to three metabolites by some strains of Escherichia coli and an unidentified Gram negative coccus. The main product which was produced with the yield of 60 per cent or more was proved to be 8-D-hydroxy tetradec-cis-5-enoic acid. This acid is colorless liquid, [α]18D+6.82° in ethanol, and its p-bromophenacyl ester melts at 47.5°C. Other two components are con-sidered to be 10-hydroxy hexadec-7-enoic and 6-hydroxy dodec-3-enoic acids.
For the isolation of these substances, sili-cic acid column chromatography, reversed phase column chromatography and thin layer chromatography were employed. It was found that the thin layer chromatography (solvent system: hexane-ethyl acetate-acetic acid; 70:30:1) was very useful for the detection of hydroxy acids and dicarboxylic acids.
We are indebted to Prof. N. Ishida of Tohoku University for his kind advice in microbiological techniques. Thanks are also due to the members of the Central Analysis Room of this Institute for the elementary analyses and infrared spectroscopy.抄録全体を表示PDF形式でダウンロード (427K) -
KAZUYOSHI HORISAKA, AKIHIKO MUSASHI1963 年 53 巻 4 号 p. 271-274
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリー1. Aus dem Walmuskel (10kg.) wurden 1.4g. Carnosin and 4.4g. Ophidin kristal-linisch dargestellt.
2. Die obengenannten Substanzen wurden durch Salzbildungen, Analyse and Papier-chromatographie respektive identifiziert.
3. Auf dem Papierchromatogramm zeigte sich, dass Lysin, Glutaminsaure, Asparagin-saure, Glycin, Serin, Threonin, Prolin, Valin, Alanin, β-Alanin, Phenylalanin, Leucin and Kreatin in freier Form vorkommen.抄録全体を表示PDF形式でダウンロード (218K) -
II. Isolation of Arabinose Mycolate and Identification of Its Chemical StructureICHIRO AZUMA, YUICHI YAMAMURA1963 年 53 巻 4 号 p. 275-281
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリー1. A new glycolipid, arabinose mycolate, was isolated from the B fraction of bound lipids of human tubercle bacillus Aoyama B strain after treatment of 0.1 N HCl at 37°C for 72 hours.
2. By acid and alkaline hydrolysis of this glycolipid, mycolic acid and D-arabinose were obtained. Mycoloyl alcohol was obtained by reductive cleavage with LiAlH4.
3. Methylation showed that one molecule of mycolic acid and one molecule of D-arabi-nose are attached through an ester linkage to 5 hydroxy group of D-arabinose.
The authors express their deep gratitude to Prof. T. Momose and Prof. H. Tsukamoto, School of Pharmacy, Kyushu University, for their helpful sug-gestion and Prof. M. Tomoda, Kyoritsu Pharmaceu-tical College for kind supplement of the samples of methylated sugar. The authors are indepted to Mrs. S. Matsuba and Mr. M. Shidoh for elementary analysis, and Mr. K. Hikita for spectral measurement. This work was supported by a grant for scientific researchs from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (496K) -
TAKESHI FUJITA, EIJI ITAGAKI, RYO SATO1963 年 53 巻 4 号 p. 282-290
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリーCytochrome b1 was solubilized from the particulate fraction of Escherichia coli with the aid of snake venom and deoxycholate and was extensively purified by ammonium sul-fate fractionation and hydroxylapatite column chromatography. The purifed preparation was still contaminated by a colorless impurity which sedimented more slowly than the cytochrome in the ultracentrifugal field; the purity being estimated to be 50 to 70 per cent. A molecular weight of 600, 000 to 800, 000 could be expected for the cytochrome from its sedimentation constant. On the other hand, a
minimum molecular weight of about 160, 000 was obtained from the heme content of the cytochrome. The cytochrome could be split by acid acetone into protoheme and an apoprotein moiety. The recombination of the two components could be observed spectro-photometrically, but the reconstructed cytochrome was no more native as evidenced by its capabiliiy to combine with CO. The normal oxidation-reduction potential of purified cytochrome bt was determined to be about -20 mV at pH 7.0 and 25°C. Spectral properties, reactivity and chemical composition of the purified preparation were also studied.
We wish to thank Dr. A. Ohsaka of the Natio-nal Institute of Health, Tokyo, for a generous gift of snake venom, and Dr. K. Kakiuchi of the Institute for Protein Research, Osaka University, Osaka, for carrying out the ultracentrifugal analyses.抄録全体を表示PDF形式でダウンロード (1335K) -
XLIX. Synthesis of 3α, 7α, 12α, 27-PentahydroxycoprostaneTAKAHIKO HOSHITA, MINORU KOUCHI, TARO KAZUNO1963 年 53 巻 4 号 p. 291-294
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリー3α, 7α, 12α, 26, 27-Pentahydroxycoprostane was synthesized.抄録全体を表示PDF形式でダウンロード (197K) -
L. Metabolism of Lithocholic Acid in Hog LiverYOSHINORI KURATA1963 年 53 巻 4 号 p. 295-298
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリーLithocholic acid-24-C14 was incubated with hog liver homogenate, and the radio-active hyodeoxycholic acid and chenodeoxy-cholic acid were isolated from the reaction mixture.
The author wishes to thank Prof. T. Kazuno and. Assist. Prof. K. Okuda for their helpful suggestion, throughout this work.抄録全体を表示PDF形式でダウンロード (216K) -
VII. Further Purification and Properties of Denitrifying EnzymeHIDEKAZU IWASAKI, SOSUKE SHIDARA, HIDEHO SUZUKI, TAKESHI MORI1963 年 53 巻 4 号 p. 299-303
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリー1. An ultracentrifugally homogeneous preparation of the denitrifying enzyme was obtained from cells of a strain of denitrifying bacterium, by the ammonium sulfate fractionation and column chromatography on Amberlite CG-50 and carboxymethyl cellulose.
2. The purified enzyme showed absorption maxima at 280 mμ with a shoulder at about 290 mμ, 594 mμ, and plateaux at about 750-780 mμ and 460-480 mμ, in the oxidized state, while no absorption was observed in the visible region for the reduced enzyme.
3. The determination of sedimentation coefficient (s20, w=6.46 S) and diffusion coeffi-cient (D20, w=4.00×10-7cm2./sec.) lead to a calculated molecular weight of 149, 000.
4. It seems likely that copper is the metal constituent of the denitrifying enzyme.抄録全体を表示PDF形式でダウンロード (1345K) -
XIII. Kinetic Studies on the Heat Denaturation of Bacterial Proteinase N' and its Complex with Diisopropyl-fluorophosphateICHITA FUKE1963 年 53 巻 4 号 p. 304-313
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリー1. The heat denaturation of BPN' and DFP- inhibited BPN' did not follow a simple first-order reaction but was a more complex reaction.
2. A study of the effect of the ionic strength of sodium chloride solution on the reaction shows that the ionic process during the heat denaturation of BPN' and DFP-inhibited BPN' in sodium chloride solution is an ion-ion reaction of ions with opposite signs.
3. A study of the effect of the ionic strength of phosphate solution on the reaction shows that the phosphate anion has two effect on the heat denaturation of BPN' and DFP-inhibited BPN'. One is a specific reaction of phosphate anion and the other is the ordinary reaction of ionic solutions.
4. During the heat denaturation of BPN' and DFP-inhibited BPN', the difference in the heat of activation is small, and the dif-ference in the entropy of activation is quite large.
The author wishes to express his sincere thanks to Prof. K. Okunuki for his continuous encourage-ment and interest during the course of this work and also to Nagase Co. for kind supplying crystalline bacterial proteinase, “Nagarse”.抄録全体を表示PDF形式でダウンロード (520K) -
RIKURO NAKAMURA1963 年 53 巻 4 号 p. 314-322
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリー1. Rat brain slices strongly concentrate L-histidine from the suspending medium. D-Histidine is also concentrated well. However there is some degree of stereospecificity between L- and D-histidine. Urocanic acid is not concentrated.
2. Active histidine uptake is markedly suppressed by dinitrophenol and in glucose-free medium.
3. L- And D-methionine inhibit the up-take of L- and n-histidine but the uptake of DL-methionine is stimulated by L-histidine.
4. When brain slices prepacked with L-histidine are incubated with methionine, a very rapid release of L-histidine takes place. On the other hand, prepacking slices with L-histidine stimulates the uptake of DL-methionine.
5. Exchange diffusion between L-histi-dine in slices and DL-methionine in the me-dium occurs in a mole-to-mole ratio.
6. The efflux rate of DL-methionine is much greater than the two isomers of histi-dine.
7. The uptake of histidine by rat brain in vivo is stereoselective.
The author is greatly indebted to Prof. M. Suda for his encouragement and advice during the work and for help in preparing the manuscript.抄録全体を表示PDF形式でダウンロード (602K) -
MINORU KAWADA1963 年 53 巻 4 号 p. 323-325
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (184K) -
KEIKO MATSUZAKI1963 年 53 巻 4 号 p. 326-327
発行日: 1963/04/25
公開日: 2008/11/18
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HIROHIKO KATSUKI, JUN NAGAI, AKIRA WADA, ISAMU FUKUMA, SHOZO TANAKA1963 年 53 巻 4 号 p. 328-330
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (172K) -
TARO KAZUNO, TAKAKO MASUI, TOSHIHIKO NAKAGAWA, KYUICHIRO OKUDA1963 年 53 巻 4 号 p. 331-332
発行日: 1963/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (711K)
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