巻号一覧
54 巻, 1 号
選択された号の論文の19件中1～19を表示しています
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III. Terminal Groups and Amino Acid Composition of the Proteolytically Modified Phenylazobenzoyl-Taka-Amylase AHIROKO TODA1963 年 54 巻 1 号 p. 1-7
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーThe dinitrofluorobenzene method has been applied to the identification and quanti-tative determination of the N-terminal amino acid of M-PhAB-TAA. It was shown that alanine was a sole N-terminal amino acid.
The carboxypeptidase technique has been applied for the characterization of the C-terminal group of the modified enzyme and it was supposed that the C-terminal region of M-PhAB-TAA was changed by proteolysis.
The amino acid composition of the modifi-ed enzyme has been determined by chromato-graphic techniques on columns of Amberlite IR-120. Separate determination has been made for tryptophan by colorimetric analysis using p-dimethylaminobenzaldehyde.
From these results, the best estimation for the amino acid residues of M-PhAB-TAA was made and compared its side chain groups with those of PhAB-TAA.
The author is grateful to Prof. S. Akabori and Prof. K. Narita for their kind guidances for this investigation. Thakns are also due to Sankyo Co., Ltd. for their kind supply of “Takadiastase Sankyo”.抄録全体を表示PDF形式でダウンロード (455K) -
XIV. Hydrolyses of Insulin B Chain and Glucagon by Proteinase c from Agkistrodon halys blomhoffii VenomMASATOSHI SATAKE, TAMOTSU OMORI, SADAAKI IWANAGA, TOMOJI SUZUKI1963 年 54 巻 1 号 p. 8-16
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーIt was presumed that proteinases a, b and c which were separated from the venom of Agkistrodon halys blomhoffii (Mamushi) by DEAE-cellulose chromatography have the different substrate specificities judging from the result of the patterns of the digest of B chain of insulin and glucagon. Some informations about the investigations of the hydrolytic site of B chain of insulin and glucagon with venom proteinase c were presented. B chain of insulin was readily hydrolyzed by proteinase c and the three hydrolytic sites, _??_ and_??_, were identified. The peptides containing arginine or lysine as the C-terminal residue, which are expected to be present in the tryptic digest, were not ob-tained. From the digest of glucagon with venom proteinase c, histidine, serine, phenyla-lanine and leucine were demonstrated as the N-terminal amino acids, and aspartic acid, threonine and presumably tryptophan as the C-terminal residues.
The authors wish to express their gratitude to Dr. T. Murachi of the Department of Biochemistry, Nagoya City University, for the kind supply of precious sample of glucagon. This work was supported, in part, by a grant for scientific researches from the Ministry of Education for “Enzymatic studies on animal toxins”, to which our thanks are due.抄録全体を表示PDF形式でダウンロード (613K) -
SHIZUO WATANABE, TERRY TROSPER, MORTON LYNN, LARRY EVENSON1963 年 54 巻 1 号 p. 17-24
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーA sensitive method for magnesium determination which utilized the fluorescence of magnesium-8-hydroxyquinoline complex made estimation of the magnesium binding constant of adenosine triphosphate possible to be carried out in solutions containing both adenosine triphosphate and magnesium ion in concent-rations as low as 10-5M. The constant for Mg2+-ATP4- was thus found to be 6-8×104 (M)-1 in 0.1M tris-(hydroxy)-methyl-amino-methane buffer (pH 8.0-8.3) and 3-5×104 (M)-1 in 0.1M triethanolamine buffer (pH 8.0-8.3) at 25°C.
The magnesium binding constants of ITP, ADP, pyrophosphate, orthophosphate, citrate, and oxalate were also estimated by the same method. It was then found that the constants for all the phosphate compounds tested were higher in Tris-buffer than in triethanolamine buffer but that the constants for citrate and oxalate were independent of the difference in buffer.抄録全体を表示PDF形式でダウンロード (570K) -
I. Fractionation, Molecular Weight Determination, Amino Acid and Fatty Acid CompositionTOMOJI SUZUKI, HIROYUKI INOUYE, KAZUO FUJIKAWA, YASUNOBU SUKETA1963 年 54 巻 1 号 p. 25-33
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーA commercial colistin was fractionated by countercurrent distribution into three different peptides. Paper chromatography showed these peptides corresponded to colistin A, B and C.
On acid hydrolysis both colistin A and B gave DAB 6, L-threonine 2, L-leucine I and D-Ieucine 1. From the ether extracts of colistin A hydrolysate MOA was isolated, and IOA from colistin B.
Molecular weights of colistins were deter-mined by the method of partial DNP-sub-stitution and spectrophotometric measurement of the picrates and the values of about 1360 were found for both colistins A and B as their pentahydrochlorides.
The authors wish to thank Mr. T. Yano and his coworkers of Banyu Pharmaceutical Co., Ltd. for the gifts of commercial colistin, and for the test of antibacterial activity. We are indebted to Mr. Y. Yoichi of Nippon Shinyaku Co., Ltd. for the gas chromatography, Dr. H. Hatano of Department of Biochemistry, Faculty of Science, Kyoto University for amino acid analysis by amino acid analyser, Mr. S. Esaka of our laboratory for microbiological assay and the members of microanalytical center of Kyoto University for elementary analysis.抄録全体を表示PDF形式でダウンロード (680K) -
II. Citramalate Metabolism in Pseudomonas fluorescens Grown on ItaconateJUN NAGAI1963 年 54 巻 1 号 p. 34-40
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーDL-l-C14-Citramalate was used to investigate the metabolic pathway of free citra-malate. By the crude extract from itaconate-grown Pseudomonas fluorescens, citramalate was first converted to citramalyl-CoA by the CoA transfer from succinyl-CoA and then citra-malyl-CoA was cleaved into acetyl-CoA and pyruvate. The activities of succinyl-CoA synthetase and CoA transferase in the glucose-grown cells were the same as the one in the itaconate-grown cells, but the activity of citramalyl-CoA lysing enzyme was increased more than 10-fold by the cultivation in the presence of itaconate.
The author wishes to express his thanks to Profs. Tanaka and Dr. H. Katsuki for their discussions and encouragements. He also thanks to Mrs. F. Katsuki for the colaboration.抄録全体を表示PDF形式でダウンロード (490K) -
LI. Isolation of 3α, 7α, 12α-Trihydroxy-Δ24-homo-5α-cholene from Bull Frog BileTAKAKO MASUI1963 年 54 巻 1 号 p. 41-46
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーIt was verified that chemical constitution of β-trihydroxyhomocholene isolated by Ku-roda (9) from bull frog bile is 3α, 7α, 12α-trihydroxy-Δ24-homo-5α-cholene.
The author wishes to express her sincere thank to Prof. T. Kazuno for his valuable suggestions throughout this work. She also thanks Prof. G. A. D. Haslewood for his most pertinent advice and for getting infrared spectra of my sample.抄録全体を表示PDF形式でダウンロード (370K) -
LIII. Synthesis and Metabolism of Cholic AldehydeHIROMASA YASHIMA1963 年 54 巻 1 号 p. 47-50
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. 3α, 7α, 12α-Trihydroxycholanic aldehyde was synthesized by lead tetraacetate oxidation of 3α, 7α, 12α, 24_??_, 25-pentahydroxy-homocholane which was prepared from 3α, 7α, 12α-trifor moxy-25-ace toxy-24-oxo-homo ch olane.
2. 3α, 7α, 12α-Trihydroxycholanic alde-hyde was oxidized to cholic acid by rat liver homogenate.
The author wishes to thank prof. T. Kazuno and Assist. Prof. K. Okuda for their helpful suggestions throguhout this work.抄録全体を表示PDF形式でダウンロード (809K) -
Effect of p-Chloronitrobenzene upon the Oxygen Affinity of HemoglobinHIROMICHI HASEGAWA, MITSUO SATO1963 年 54 巻 1 号 p. 51-57
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリー1. When rabbits received 0.5g. of p-chloronitrobenzene per Kg. of body weight, the formation of methemoglobin and Heinz bodies was observed as reported by many investigators.
2. It was found that p-chloronitrobenzene or its metabolites cut off the interaction among the four hemes of the hemoglobin molecule concerning the combination of oxygen by com-bining irreversibly with the protein part of the hemoglobin molecule and that the affinity of hemoglobin of poisoned rabbits for oxygen increased. This functional change of hemoglobin molecule has a conciderable physiolo-gical consequences.
3. It was observed that the catalase ac-tivity in blood decreased considerably by the injection of p-chloronitrobenzene.
The authors wish ot express their gratitude to Drs. H. Sakabe and M. Yamaguchi for their guidace in this work.抄録全体を表示PDF形式でダウンロード (511K) -
Effect of Nitroglycol upon the BlooodHIROMICHI HASEGAWA, MITSUO SATO1963 年 54 巻 1 号 p. 58-64
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーRabbits received subcutaneously 0.3g. of nitroglycol per kg. of the body weight every day, and the blood was punctured from the heart at the intervals of one or two hours after the injection. Using this blood, the mechanism of methemoglobin formation, the oxygen affinity of hemoglobin and the catalase activity were investigated.
1. The methemoglobin content was plotted with time after the injection, and it was found that the ratio of methemoglobin to total hemoglobin was about 50 per cent at a maximum, but it recovered to the normal level at 24 hours after the injection. From this fact, the reducing power in the blood, by which methemoglobin was converted into hemoglobin, was found to be very strong. The methemoglobin formed in vivo by the injection of nitroglycol was not NO2-methemo-globin.
2. The oxygen affinity of hemoglobin of poisoned animals increased with time after the injection and recovered to the normal at 24 hours. The mechanism of the increment of the oxygen affinity was interpreted by the following equation;
Hb+nitroglycol_??_Hb•itroglycol
3. The catalase activity in the blood decreased with time after the injection and recovered partially at 24 hours. It was concluded that the decrease of catalase activity was caused by the reversible inhibitory action of an un-known substance accumulated in the blood of poisoned animals.
The authors wish to express their graitude to Drs. M. Yamaguchi and H. Sakabe for their guidance in this work.抄録全体を表示PDF形式でダウンロード (534K) -
HACHIRO NAKAGAWA, HIDEO INOUE, YOSHIRO TAKEDA1963 年 54 巻 1 号 p. 65-74
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーA pyrocatechase was isolated from a strain of Brevibacterium fuscum and purified about 40-fold. This enzyme was homogeneous by ultracentrifugation. Its molecular weight was estimated as 78, 000.
Brevibacterium pyrocatechase has unique substrate specificity. Thus it catalyzes the oxidative cleavage of the benzene ring of various catechols including 3- and 4-methyl-catechols and pyrogallol unlike the pyrocate-chases from other bacterial species.
The purified enzyme requires both ferrous ion and a reducing agent for maximal activity.
The reaction products from catechol, 3-and 4-methylcatechols and pyrogallol were identified as cis, cis-muconic acid, α- and β-methylmuconic acids and α-hydroxymuconic acid, respectively.
It was also demonstrated that the substrate specificity of the enzyme obtained from Brevibacterium fuscum was the same on what-ever metabolic precursors of catechol the bacteria were grown.
We are indebted to Dr. S. Senoh for his many stimulating discussions and suggestions during the course of this work.抄録全体を表示PDF形式でダウンロード (1453K) -
YUMIKO UENO, KEIKO HAYASHI, RYOITI SHUKUYA1963 年 54 巻 1 号 p. 75-80
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーApokynurenine transaminase has been purified to 380 fold from horse kidney and its properties have been studied. The Michaelis constants for L-kynurenine, α-ketoglutarate, pyridoxal phosphate and pyridoxamine phos-phate have been evaluated to be 1.8×l0-3M, 1.0×10-4M, 1.4×10-6M and 2.6×10-6M, respectively. Optimum pH was 6.5. It has been able to show individual half-reactions of kynurenine transaminase.
The authors wish to express their appreciation to Dr. K. Kaziro for his interest in and support of this research and are indebted to Miss S. Iimura for her competent assistance. This work was supported by a Grant for Scientific Research from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (399K) -
IV. Transfer of Nuclear Nucleoprotein into CytoplasmAKIKO MORIYAMA, MINORU MITSUHASHI, HIDESATO OHTSUKA, SHOKO OHMURA, YOS ...1963 年 54 巻 1 号 p. 81-88
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーEvidence is presented for the transfer of labeled nuclear RNA or ribonucleoprotein to unlabeled cytoplasm in reconstituted hornogenate of hepatoma cells. The transferred RNA or ribonucleoprotein was found in the upper layer of sucrose density gradient centri-fugation as well as in the elute with low concentration of NaCI from DEAE cellulose column.
For the transferring mechanism, positive role of cytoplasmic factor was suggested. The nuclear RNA or ribonucleoprotein transferred to the cytoplasm stimulates amino acid incorporation in microsomes.
This study was supported research grants from the National Institute of Health, U.S.A. (CY 5869 CY).抄録全体を表示PDF形式でダウンロード (546K) -
II. Regeneration of Urea Denatured Enzyme by Removal of Dilution of UreaAKIRA IMANISHI, KINJI KAKIUCHI, TOSHIZO ISEMURA1963 年 54 巻 1 号 p. 89-96
発行日: 1963年
公開日: 2008/11/18
ジャーナル フリーThe molecular conformation of bacterial α-amyase, which contains neither disulfide linkage nor sulfhydryl group, is extensively unfolded in 8M urea solution, is extensively unfolded in 8M urea solution, containing 1/400M EDTA. The reversal of the urea denatured enzyme has been examined by means of dilution or removal of urea. Regeneration of the enzymatic activity depends on pH values and ionic strength of the solution, and concentration of the enzyme. The reversal of the stable conformation of the enzymatical-ly reactivated enzyme has also been examined by spectrophotometric titration in alkaline region in comparing with those for the native and denatured enzyme. The results obtained suggest that under an appropriate condition regeneration of the enzymatically active bacterial α-amylase should be possible, so far as the molecule does not suffer any chemical modification during process of denaturation.
The authors wish to thank Nagase Sangyo Co. Ltd. for supplies of crystalline bacterial amylase.抄録全体を表示PDF形式でダウンロード (587K) -
KYUICHIRO OKUDA, TAKAKO MASUI, TAKAHIKO HOSHITA, TARO KAZUNO1963 年 54 巻 1 号 p. 97-98
発行日: 1963年
公開日: 2008/11/18
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YOSHIMI OKADA, SHOICHI NAKASHIMA, YUICHI YAMAMURA1963 年 54 巻 1 号 p. 99-100
発行日: 1963年
公開日: 2008/11/18
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YOSHIMI OKADA, TOKUJI IKENAKA, TAKAYASU YAGURA, YUICHI YAMAMURA1963 年 54 巻 1 号 p. 101-102
発行日: 1963年
公開日: 2008/11/18
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MAKOTO SEIJI, SADAO IWASHITA1963 年 54 巻 1 号 p. 103-106
発行日: 1963年
公開日: 2008/11/18
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TOSMOHARU OKUDA, MASARU TAKESHITA, HIROKO NAKAMURA, YUKIO ITO1963 年 54 巻 1 号 p. 107-108
発行日: 1963年
公開日: 2008/11/18
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YOSHIO MATSUSHIMA, TSUYOSHI MIYAZAKI, JAMES T. PARK1963 年 54 巻 1 号 p. 109-110
発行日: 1963年
公開日: 2008/11/18
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