巻号一覧
57 巻, 2 号
選択された号の論文の23件中1～23を表示しています
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JIRO KOYAMA1965 年 57 巻 2 号 p. 103-108
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリー1. Studies were done on kinetics of hemolysis induced by purified streptolysin S'. An examination of the kinetics of hemolysis revealed the existence of lag phase and that after the lag phase hemolytic rate was pro-portional to streptolysin S' concentration. Hemolytic rate was found to be decreased as
the initial erythrocyte concentration became higher.
2. Interaction of streptolysin S' with erythrocytes during the lag phase was exa-mined. There was always a large part of streptolysin S' left in liquid phase during the lag phase. The erythrocytes pretreated with the toxin at 10°C were found to hemolyze at. 37°C.
3. Lecithin and stromata, but not chole-sterol, inhibit the hemolysis by the toxin.
The present author wishes to express his sincere thanks to Prof. F. Egami for his encouragement during this work. He also expresses his thanks to Dr. S. Nojima of the University of Tokyo for his kind supply of purified egg yolk lecithin.抄録全体を表示PDF形式でダウンロード (404K) -
YUKIFUMI NAGAI, YASUKAZU AKITA1965 年 57 巻 2 号 p. 109-115
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリー1. The relationship between the ATPase-inhibitory activity and the ATPase activity of the relaxing granules was examined.
2. The treatments such as standing at 37°C, prolonged standing at 0°C and addition of DOC, decreased the ATPase-inhibitory activity concurrently with the increase of the ATPase activity.
3. DNP did not affect these two activities of the granules.
4. Oxalate potentiated the ATPase-in-hibitory activity of aged granules and also simultaneously suppressed their high ATPase activity. ‘Stimulated’ by the treatments, the ATPase was not affected by addition of oxalate.
5. The enzymatic properties between the ‘latent’ and ‘stimulated’ ATPase were com-pared. No marked changes were not observed and the stimulation due to the treatments was limitted to Mg++-dependent ATPase.
6. It was concluded that the relaxing granules were effective in inhibiting myofibrillar ATPase only when the granular ATPase was kept low.抄録全体を表示PDF形式でダウンロード (539K) -
KEIZO SUZUKI, YOSHIAKI MIYOSAWA1965 年 57 巻 2 号 p. 116-124
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーThe denaturation and the renaturation of bovine serum γ-globulin under high pressure were studied using solubility as a measure of denaturation. The coagulation of isoelectric γ-globulin is initiated by compression around 4, 000kg./cm.2 and completed by compression for 5 minutes at 8, 000kg./cm.2. The depression effect of pressure on the heat denaturation at 70°C has a maximum at about 2, 000kg./cm.2. The temperature coefficient of pressure denatu-ration is positive up to 50°C. The rate of pressure coagulation is more rapid when the pH of the protein solution (pH 5 to 10) is lower, and the initial protein concentration is higher.
The most striking result differing from ovalbumin and carbonyl hemoglobin is the fact that the amount of coagulated protein levels off after a certain pressure duration, and the amount of soluble protein in this final state depends upon the magnitude of pressure and temperature. Some physico-chemical properties of the soluble protein are different from those of the native protein, although the differences are small. It may be assumed that there is an equilibrium between soluble protein and coagulated pro-tein, although the reverse process (dissolution) is not so readily established.
The dissolution of partially pressure-coagulated γ-globulin is remarkably accel-erated by recompression at moderately high pressure, and the rate increases with tempera-ture up to 40°C. Some physico-chemical properties of completely dissolved protein are assumed not to be the same as those of the native protein. It was not possible to cause the completely pressure-coagulated sample (100% denatured) to go into solution. However, partially pressure-coagulated γ-globulin dissolves more readily than heat-coagulated γ-globulin.抄録全体を表示PDF形式でダウンロード (640K) -
TAKAHIRO KUWAKI1965 年 57 巻 2 号 p. 125-130
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーSynthetic samples of S-(isopropylcarboxy-methyl) glutathione (GSIV) are converted into isovalthine by guinea pig kidney homogenate or glutathionase preparations.
The cleavage reaction is accelerated by addition of glutamine or methionine to almost the same extent. The amount of glycine liberated, but not that of glutamic acid, is fairly proportional to that of isovalthine formed.
(+)-GSIV synthesized from (+)-α-bromo-isovaleric acid and glutathione is a better substrate for guinea pig kidney glutathionase than (-)-GSIV synthesized from (-)-α-bromo-isovaleric acid and glutathione. (+)-GSIV gives only L-isovalthine and (-)-GSIV only L-allo-isovalthine after treatment with gluta-thionase.
The author is indebted to Prof. Dr. S. Mizuhara for his interest.抄録全体を表示PDF形式でダウンロード (1298K) -
II. Reactions Catalyzed by Protein KinaseHIDEO MATSUI, EIJI ORIKABE, SHINJI ISHIKAWA, NORIO SHIMAZONO1965 年 57 巻 2 号 p. 131-141
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリー1. The protein kinase attached to “residue” particles, which were prepared from ox brain acetone-butanol powder, was able to catalyze the transphosphorylation from ATP32 not only to the residue phosphoprotein, but also to added casein. The soluble kinase was also able to phosphorylate the heat-treated and untreated residue. However, the phosphory-lation reaction of residue phosphoprotein by the residue kinase was found to be the most rapid.
2. General properties of the residue phos-phorylation were studied. Transfer of γ-P of ATP was confirmed. Km for ATP was 1.2×10-4M. The dependence on Mg++ was shown, and its optimum concentration was 5mM. Other divalent cations inhibited the activity. Optimum pH was about 7.4. These properties were similar to those of the soluble enzyme. Na+ and K+ ions inhibited the activity of the residue kinase, whereas that of the soluble enzyme was stimulated two fold by 0.1 M Na+ and K+. PCMB, at a concentration of 10-4M, inhibited completely the residue phosphory-lation, but it did not inhibit the soluble enzyme completely even at the concentration of 10-3M.
3. More than 80% of the activity of protein kinase was found in the particulate fraction. The activity of the particulate phos-phorylation was found mainly in the mito-chondrial and microsomal fractions. A phos-phoprotein which could be phosphorylated by protein kinase showed the same distribution as that of the particulate phosphorylation.
4. The activity of Na+, K+-activated ATPase was also found in the “residue” parti-cles. The velocity of hydrolysis was 25 times faster than that of phosphorylation.
5. The significance of the properties of the particulate phosphorylation has been dis-cussed, in relation to the rapid turnover of phosphoprotein phosphorus in vivo.抄録全体を表示PDF形式でダウンロード (734K) -
MASACHIKA IRIE, TYUNOSIN UKITA1965 年 57 巻 2 号 p. 142-146
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーA modification of the assay methods for phosphomono-or di-esterase activity using p-nitrophenyl or bis (p-nitrophenyl) phosphate as substrate was presented.
The method is based on the direct reading of the spectral difference between substrate and products.
The fundamental data for this method were presented and the possibility of its application to kinetic studies of phospho-mono- and di-esterases without taking out any aliquot from the reaction mixture from pH 2 through 12 was discussed.抄録全体を表示PDF形式でダウンロード (299K) -
SHIGEO HORIE1965 年 57 巻 2 号 p. 147-154
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーLow molecular-weight heme a compounds as spectral models for cytochromes a and a3 were studied.
1. Heme a imidazole ferrihemochrome and ferrohemochrome were found to be good spectral models for oxidized and reduced forms of cytochrome a, respectively.
2. Hemin a dissolved in histidine solution around neutral pH showed the spectrum which was similar to the oxidized form of cyto-chrome a3.
3. Heme a dissolved in 20% glutamate solution containing 2% of Tween 20 at pH 7.0 showed the reduced and CO-bound spectra having the essential characteristics of reduced and CO-bound spectra of cyto-chrome a3. Although this model was not completely satis-factory, it was better than the models hitherto reported.
4. It was thus demonstrated that heme a could show a- and a3-type spectra depending upon the conditions employed. The difference in 5, 6-coordination stapes of cytochromes a and a3 were discussed.
The author express s his cordial thanks to Prof. N. Shimazono for his ind advice and encouragement. The author wishes also to send his heartful thanks to Prof. K. Shibata otthe Tokyo Institute of Technology for valuable advices.抄録全体を表示PDF形式でダウンロード (541K) -
SUSUMU TSURUFUJI, SADAHIKO ISHIBASHI1965 年 57 巻 2 号 p. 155-160
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーCollagen biosynthesis was studied in vivo with special reference to glycine metabolism.
For the purpose, separation of almost all major amino acids in collagen was examined, and a method was established combining group separation of the amino acids by ion exchange chromatography, and separation of the dinitrophenylated amino acids by Celite column chromatography and paper chromato-graphy.
Guinea pigs of different ages were injected with 2-C14-glycine and sacrificed after 3 or 24 hours. Acetic acid extractable skin collagen and serum albumin, which was used as a control protein, were obtained from the animals and purified. After hydrolysis, amino acids of the two proteins were isolated and estimated for their radioactivities. Difference was observed between the two proteins in the pattern of labeling, especially in the ratio of the specific activity of glycine to that of serine, the ratio being 3-5 for collagen and about l for serum albumin, irrespective of the age of the animals and experimental period. The difference of the specific activity ratio may be due to the marked requirement of glycine for collagen biosynthesis, because collagen contains extraordinarily large amount of glycine. Re-lationship between protein biosynthesis and amino acid supplying system was considered.
The authors are grateful to Mr. Y. Mitsui for his co-operation.抄録全体を表示PDF形式でダウンロード (432K) -
HIROSHI HYODO, IKUZO URITANI1965 年 57 巻 2 号 p. 161-166
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーo-Diphenol oxidase [EC 1. 10. 3. 1] activity in sweet potato roots infected with the black rot fungus was fractionated by DEAE-cellulose column chromatography to three fractions, called components I, II and III. The enzyme in the cut and healthy tissues was found to be composed of II and III, and II, re-spectively. Component III was different from component II in terms of immunological and electrophortic behaviours. Two components were detected in component III by starch-gel electrophoresis which were identical with each other by the immunoelectrophresis.
The authors are indebted to Professor M. A. Stahmann for his valuable advice for preparing this manuscript. They are also grateful to Dr. T. Tomita for his helpful aid in the immunological experiments and Dr. T. Asahi for his constant discussions through this work.抄録全体を表示PDF形式でダウンロード (476K) -
III. The Effects of pH and Salts on the Monomer-Dimer TransformationKINJI KAKIUCHI, KOZO HAMAGUCHI, TOSHIZO ISEMURA1965 年 57 巻 2 号 p. 167-175
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリー1. The change in the equilibrium between monomer and dimer of Bacillus subtilis α-amylase with pH from 6 to 9 was examined by the sedimentation velocity experiments. It was found that the slower moving com-ponent in the sedimentation pattern increases with increasing pH.
2. The monomer-dimer transformation of B. α-amylase was studied at various conditions by light scattering measurements. The value of Kc/R90 depended upon the protein con-centration. In 0.1M NaCl-0.005M calcium acetate, Kc/R90 was independent of the protein concentration when the protein concentration was more than 0.5%. The molecular weight calculated from the values of Kc/R90 in this region was found to be 97, 600, the molecular weight of the dimer. As the value of pH increases the equilibrium shifted toward the monomer. This was in accord with the results of the sedimentation measurements. Treat-ment with calcium-EDTA at pH 7.0, however, did not cause a perfect dissociation of the dimer into the monomer and the dimer remained slightly.
3. B. α-amylase treated with sodium-EDTA at pH 7.0 was studied by the Archibald method and by optical rotatory dispersion. For at least three days of the treatment, the inhomogeneity in the molecular weight was not observed and the molecular weight was found to be 48, 200. However, the optical rotatory dispersion constant decreased gradually during the incubation.
4. An attempt to analyze the light scat-tering data was made in order to evaluate the equilibrium constant between monomer and dimer. The equilibrium constant was estimated to be 2×109 mole-2 liter2.
The authors would like to express their thanks to Nagase Sangyo Co., Ltd. and Daiwa Kasei Co., Ltd. for supplying Bacillus subtilis α-amylase.抄録全体を表示PDF形式でダウンロード (2211K) -
KOZO NARITA, NOBUO SATO, KIKUO OGATA1965 年 57 巻 2 号 p. 176-183
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーActivation of sixteen acetylamino acids by rat liver pH 5 enzyme fraction was studied. When the assay was made by pyrophosphate-ATP exchange reaction, only acetylmethionine and acetylleucine were apparently active. However no incorporation of radioactivity of acetyl-C14-L-methionine into s-RNA could be observed, and radioactive acetate was identified from the reaction mixture, indicating the presence of an acetylase in the crude enzyme preparation. Thus the conclusion that acetyl-amino acids examined could not be activated by the rat liver enzyme, was presented.
The authors are grateful to Dr. H. Asoka for his help in part in the presnt studies. The present work has been aided in part by a grant from the Ministry of Education for which the authors are grateful.抄録全体を表示PDF形式でダウンロード (577K) -
II. Amino Acid Activation, Formation of Amino Acyl s-RNA, and Transfer of Amino Acids from s-RNA to Microsomal Protein in Brain Cell-free SystemMEI SATAKE, YASUO TAKAHASHI, KEIKICHI MASE, KIKUO OGATA1965 年 57 巻 2 号 p. 184-191
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーThe presence and enzymatic characters of each step in protein biosynthetic cell-free system of guinea pig brain were investigated.
1. Considerably high amino acids depen-dent PP32-ATP exchange activity, comparable to that of liver cell sap, was observed in brain cortex cell sap, although P32-ATP exchange was not demonstrable. Among three subfrac-tions of cell sap, the pH 4.5 fraction was found to be most active.
2. Amino acids are divided into three groups depending upon their efficiency on PP-ATP exchange by brain cell sap. Valine, tryptophan, methionine, alanine, threonine and lysine belong to the very effective amino acid group: tyrosine, histidine, glycine, serine, and isoleucine belong to the effective amino acid group: leucine, phenylalanine, proline, hydroxyproline, aspartic acid, asparagine, glu-tamic acid, glutamine, arginine, and γ-amino-butyric acid belong to the little or non-effec-tive amino acid group.
3. Active C14-leucine, and C14-valine in-corporation into s-RNA occurred in a system consisting of the precipitate of brain cell sap at pH 4.5, ATP and Mg++. Transfer of C14-glutamic acid to s-RNA was observed to be less active.
4. Existence of the transfer reaction of G14-leucine from s-RNA into microsomal pro-tein was observed in the presence of cell sap. This transfer was completed by about 10 min. ATP, ATP generating system and GTP were required for the transfer. Among three cell sap fraction pH 5.0 fraction was most active, followed by pH 4.5 fraction. The simultaneous addition of an excess amount of C12-leucine did not inhibit the transfer, although the leucine attachment to s-RNA reached its maximum at about one minute of incubation. The fact suggests very rapid rate of attachment of amino acyl s-RNA to microsomal templete. This attachment is followed by the peptide bond formation of more slow reaction rate.
5. These results may indicate that a series of enzymatic reactions: amino acid activation, attachment to s-RNA and transfer into microsomal protein, is actively operated in brain tissue.抄録全体を表示PDF形式でダウンロード (618K) -
KAZUKO SEKIYA, SANAE MII, YUJI TONOMURA1965 年 57 巻 2 号 p. 192-200
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーMyosin was photooxidized by the method of Weil et al. in the presence or the absence of Mg-ATP, Mg-PP or PCMB, and a kinetic analysis on time-course of change in ATPase activity was made. On the photooxidation, Ca-activated ATPase activity increased initially to a two fold of the original and then de-creased. The rate of activation of ATPase on the photooxidation was unsignificantly changed, but that of inhibitson decreased with the addition of Mg-ATP or Mg-PP. When myosin was treated with 3.5 moles PCMB pe 105g. protein before the photooxidation or when TP was used as a substrate of enzymatic activity, only a decrease in the activity was observed on the photooxidation.
Two moles of SH groups per 105g. of myosin reacted repidly with dithiodiglycolic acid, resulting in four fold increase in Ca-activated ATPase and in disappearance of EDTA-activated ATPase activity. When myosin was treated with an excess of the reagent for a long time, Ca-activated ATPase disappeared, remaining 2 moles of unreacted SH groups per 105g. myosin and the activity restored almost to the original with the further treatment with an excess of β-mercaptoethanol. Treatment of myosin with crude β-mercapto-ethanol or with L-ascorbic acid led to decrease in the SH content of myosin by one mole per 105g. myosin, which induced a marked en-hancement of Ca-activated ATPase.
The histidine content of fully disulfide exchanged myosin as above decreased with the photooxidation, and ATPase activity, measured after recovery of SH groups, disap-peared with loss of histidine residue by 25 per cent of the control. Effects of the photo-oxidation on the reduced viscosity and the -b0 term of the disulfide exchanged myosin were measured after recovery of SH groups by the treatment with an excess of β-mercapto-ethanol, and their marked changes were observed with the decrease in ATPase activity.
On the basis of these results and others, it was concluded that the histidine residues could be located near the active site but not involved in the active site itself of myosin ATPase.
We would like to express our sincere thanks to Dr. S. Kubo of our laboratory for his skillful analysis of amino acid composition of modified myosin. This work was supported by a Public Health Service Research Grant AM-08303 from the National Institute of Arithritis and Metabolic Diseases, U.S.A., and by grants from Toyo Rayon Foundation and from the Ministry of Education of Japan to the Research Group on “Cell Motility”.抄録全体を表示PDF形式でダウンロード (691K) -
KUNIO MATSUI1965 年 57 巻 2 号 p. 201-206
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーA yellowish green fluorescent substance was extracted from the choroid of cat's eye and was partially purified. The substance had the general properties of a flavin. Its absorption spectrum closely resembled that of riboflavin. The substance was distinguishable from riboflavin, its nucleotides, glucoside, and known photodecomposition products by its behaviour on paper chromatography. The substance was thought to be a new flavin compound, and was named “nekoflavin”.
The author would like to express his thanks to Prof. S. Otani, Emeritus Prof. Y. Hosoya, and Assistant Prof. Y. Saito for their advice, and to Prof. K. Uehara for his gifts of erythro- and threoflavin. He also thanks Miss K. Yaeno, Miss M. Kinoshita, Miss K. Yamaguchi and Miss A. Yokoi for their technical assistance.抄録全体を表示PDF形式でダウンロード (437K) -
II. Purification and Properties of Sulfite Reductase from Allium odorumGORO TAMURA1965 年 57 巻 2 号 p. 207-214
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーA sulfite reductase from A. odorum leaves was purified 193-fold and its properties were detemined. The optimal pH of the enzyme was 7.0 to 7.5 in phosphate and Tris-HCI buffers. The Km for sulfite was 6.3×10-4M. The enzymatic activity was inhibited by the addition of KCN. The enzyme catalyzed the conversion of sulfite to sulfide, both of which have been known as intermediates in microbial biosynthesis of cysteine from sulfate. It was suggested that cysteine biosynthesis from sulfate in higher plants, like in micro-organisms, proceeds through sulfite and sulfide as intermediates.
The author wishes to express his sincere gratitude to Prof. R. Sato, Dr. T. Nakamura and Mr. A. Yoshimoto of Institute for Protein Research Osaka University, for their guidance; this study has been partly carried out at this institute. The author is indebted to Dr. Y. Kakiuchi for the sedimentation experiments. Thanks are also due to Prof. M. Akino, Prof. M. Hasegawa and Assistant Prof. E. Onishi in Tokyo Metropolitan University for their valuable criticism and for reading manuscript.抄録全体を表示PDF形式でダウンロード (1501K) -
HIROSHI YOSHIDA, FUJIO EGAMI1965 年 57 巻 2 号 p. 215-220
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリー1. Mucous gland extract of Charonia lampas has been found to form S35-PAPS from inorganic S35-sulfate and ATP.
2. Mucous gland homogenate incorporat-ed S35-sulfate into charoninsulfuric acid when incubated with S35-PAPS.
3. Over 95% of the incorporated S35 was present in S-rich fraction of charoninsulfuric acid.
4. Any intermediate in the transfer of sulfate from PAPS to charoninsulfuric acid could not be found.
5. The mucous gland contains “PAPS-sulfatase”, an enzyme liberating sulfate from PAPS.
We thank Seikagaku Kogyo Co. for supplies of Charonia lam pas and other supports. This work was supported in part by a grant from the Ministry of Education.抄録全体を表示PDF形式でダウンロード (427K) -
SHIGEKI MINAKAMI, TAKAYASU SAITO, SHOJI KUME, HARUHISA YOSHIKAWA1965 年 57 巻 2 号 p. 221-222
発行日: 1965/02/25
公開日: 2008/11/18
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TAKASHI MURAMATSU1965 年 57 巻 2 号 p. 223-225
発行日: 1965/02/25
公開日: 2008/11/18
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TOMOJI SUZUKI, KYOZO HAYASHI, KAZUO FUJIKAWA, KYOZO TSUKAMOTO1965 年 57 巻 2 号 p. 226-227
発行日: 1965/02/25
公開日: 2008/11/18
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GENJI MATSUDA, TOMOYUKI MAEKAWA, YOSHIAKI OTSUBO1965 年 57 巻 2 号 p. 228-229
発行日: 1965/02/25
公開日: 2008/11/18
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TATSUHISA YAMASHITA1965 年 57 巻 2 号 p. 230-231
発行日: 1965/02/25
公開日: 2008/11/18
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SATOSHI YAMASHITA, SHINJI ISHIKAWA1965 年 57 巻 2 号 p. 232-234
発行日: 1965/02/25
公開日: 2008/11/18
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Stimulation of ATPase Activity by Deoxyribonucleic Acid in the Micrococcus lysodeikticus PreparationSHINJI ISHIKAWA, SATOSHI YAMASHITA, SHUN-ICHI ARAKI, NORIO SHIMAZONO1965 年 57 巻 2 号 p. 235-237
発行日: 1965/02/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (184K)
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