巻号一覧
57 巻, 3 号
選択された号の論文の17件中1～17を表示しています
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TSUNEO KAMEYAMA, NORIO SHIMAZONO1965 年 57 巻 3 号 p. 339-345
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. The presence of ribulokinase in animal liver was demonstrated. The enzyme was purified about 100-fold from guinea pig liver.
2. Mg++ and ATP were essential for the enzyme reaction. Maxinal activity was given at the 2:1 concentration ratio of Mg++ to ATP.
3. D-ribulose 5-phosphate was produced by this reaction as reported previously. The stoichiometrical relationship was proved between the production of ADP and the decrease of ATP and D-ribulose.
4. This enzme was specific for ribulose among the substrates tested.
5. The optimal pH of this enzme was 8.5. The Michaelis constants were calculated to be 7.15×10-4 and 2.86×10-4 mole per liter for D-ribulose and ATP, respectively. The reaction was inhibited by the SH-blocking reagents.
The author wishes to express his gratitude to Dr. Y. Mano for his continuous interest and kind help throughout the investigation.抄録全体を表示PDF形式でダウンロード (508K) -
I. Phosphorylation of Bound Phosphopeptide and its IsolationEIJI ORIKABE, HIDEO MATSUI, SHINJI ISHIKAWA, NORIO SHIMAZONO1965 年 57 巻 3 号 p. 346-354
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. A convenient system for the specific incorporation of phosphate fo phosphoprotein of the brain in the cell free level was described.
2. The extraction of a phosphopeptide retaining the capacity as a substrate for protein kinase was achieved and its characteristics were found to resemble those of phosvitin.
3. O-phosphoserine was isolated from the radioactively phospholylated phosphopeptide, and was assumed to be sole radioactive organic phosphate in the peptide.
The authors are indebted to Dr. M. Yoshida, Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo for the bioassay of inositol, and to Dr. S. Tamura in the Food Research Institute for analysis of the amino acid composition of extracted phosphopeptide.抄録全体を表示PDF形式でダウンロード (584K) -
MASACHIKA IRIE1965 年 57 巻 3 号 p. 355-362
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. The effect of univalent salts on the reaction of bovine pancreatic RNase was studied and the univalent anions were found to inhibit the enzyme competitively at high salt concentrations (above 0.1M). Ki values of various univalent salt at pH 5.0 were determined.
2. pH-profile of initial velocity was studied using RNA, cytidine-2', 3'-cyclic phosphate and cytidylyl uridine as substrates. With RNA, marked shift of pH optimum was observed depending on the salt concentrations and the shift was found to be from pH 7.5 at μ=0.1 to 6.0 at μ=1.0. The shift of optimal pH, when low molecular substrate was used, was less than 0.5 pH unit.
3. A hypothesis was proposed that poly-anionic substrate can bind, in addition to the specific site which is effective for low molecular weight substrate, the non-specific binding site, presumably protonated base in protein. The non-specific bindings were readily cleaved at high salt concentrations.
4. Ki values of enzyme-inhibitor (cytidine 2'-phosphate) complex were measured at vari-ous salt concentrations using both RNA and cytidine-2', 3'-cyclic phosphate as substrates. The Ki values measured using eytidine-2', 3'-cyclic phosphate as a substrate were found to increase with the increase of salt concentrations, but those measured using both RNA and cytidine-2', 3'-cyclic phosphate as substrate were almost identical at high ionic strength. The phenomenon may be understood as follows: the binding between the polyanion (RNA) and the non-specific site of the enzyme may interfere with that between the inhibitor of low molecular weight and the specific site of the enzyme.
The author is grateful to Prof. T. Ukita for his encouragement and helpful discussions and to Dr. T. Tsumita of the Institute for Infectious Diseases of this university for affording the use of automatic recording spectrophotometer.抄録全体を表示PDF形式でダウンロード (554K) -
NARIMASA SAITO1965 年 57 巻 3 号 p. 363-371
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. Glucokinase was purified from Br. fuscum to the extent of 113-fold over cell-free ex-tracts, and the properties of the enzyme were examined.
2. The kinetic studies of the reaction were investigated.
The author wishes to express his sincerest thanks to Prof. K, Arima and Prof. Y. Ikeda of the University of Tokyo for their kind guidances throughout this work.
Great indebtedness is also acknowledged to Dr. S. Mizushima for his valuable suggestions. The author also wishes to thank Dr. M. Mogi and Dr. Iguchi for their kind advices and encouragements.抄録全体を表示PDF形式でダウンロード (583K) -
KENICHI KASAI1965 年 57 巻 3 号 p. 372-379
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. Inactivated RNase T1, in which the disulfide bonds had all been reductively cleaved by β-mercaptoethanol in 8M urea, could be reactivated completely by oxidation with air.
2. Reoxidized RNase T1 was indistinguishable from native RNase T1 in its biochemical and physico-chemical properties. This suggests that the disulfide bonds were reformed in the correct positions and that the conformation of the native protein molecule was completely regained.
3. Reactivation of reduced RNase T1 was fast at neutral pH and in the presence of sodium chloride. Variation in the protein concentration and temperature had no significant effect on the reactivation.
4. The presence of various chemicals and of other proteins had relatively small effect in contrast to the case with RNase I. This suggests that the difficulty of reactivation of the enzyme is correlated with the number of inter-nal disulfide bonds.
5. Protein molecules having disulfide bonds in abnormal positions were formed on oxidation of reduced RNase T1 in 8M urea, but these could be converted to normal molecules by reoxidation after removal of urea in the presence of β-mercaptoethanol.抄録全体を表示PDF形式でダウンロード (622K) -
XV. Separation of Arginine Ester Hydrolase of Agkistrodon halys blomhoffii Venom into Three Enzymatic Entities: “Bradykinin Releasing”, “Clotting”TADASHI SATO, SADAAKI IWANAGA, YUKIO MIZUSHIMA, TOMOJI SUZUKI1965 年 57 巻 3 号 p. 380-391
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. Venom of the snake Agkistrodon halys blomhoffii contains three or more AE hydrolases which were fractionated on a column of DEAE-cellulose.
2. The substrate specificities of all these AE hydrolases were strictly directed to the hydrolysis of ester linkages to which an arginine residue contributed the carbonyl group.
3. A “bradykinin releasing enzyme” which was almost free from clotting activity was obtained. The units of AE hydrolytic activity of the purified enzyme amounted to about 5 per cent less than the total units in the venom. There were large amounts of AE hydrolytic enzymes which were not related to the bradykinin releasing activity in the venom.
4. A considerable amount of AE hydro-lase, which did not show any “bradykinin releasing” and “clotting” activities was found in the venom. This enzyme, when injected into rabbits, induced an intense increase in capillary permeability.
5. A considerable amount of AE hydro-lase with “clotting activity” was obtained.
6. The “clotting enzyme” and “capillary permeability increasing enzyme” were purified to an ultracentrifugally and electrophoretically homogeneous state.
We are greatly indebted to Dr. D. F. Elliott of the National Institute for Medical Research, Mill Hill, London for the sample of synthetic bradykinin. We also wish to express our thanks to Mr. T. Omori of this Laboratory for carrying out capillary permea-bility tests and to Dr. K. Kakiuchi of this Institute for doing the ultracentrifugal examinations.抄録全体を表示PDF形式でダウンロード (1600K) -
XVI. Demonstration of a Proteinase with Hemorrhagic Activity in the Venom of Agkistrodon halys blomhoffiiSADAAKI IWANAGA, TAMOTSU OMORI, GENICHIRO OSHIMA, TOMOJI SUZUKI1965 年 57 巻 3 号 p. 392-401
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリーThe venom of Agkistrodon halys bloemhoffii (Japaneses snake “Mamushi”) has two hemor-rhagic proteins, one of which is identical with proteinase b. Proteinase b was purified by column chromatography on anion exchange cellulose derivatives, sephadex and hydroxyl-apatite. The yield of purified proteinase b protein from lyophilized crude venom was 2.5 per cent. The purified preparation of this enzyme appeared to be chromatographically and electrophoretically homogeneous. The results of studies on the effect of heat treatment and inhibition by EDTA and cysteine, suggest that the hemorrhagic activity of the venom is due to the action of proteinase b. Moreover, the hemorrhagic and proteinase b activities could not be separated by chromatographical treatments. The purified proteinase b was an acidic protein and its sedimentation constant was calculated to be s20=4.82×10-13.
The authors express their thanks to Drs. T. Takagi and K. Kakiuchi of this Institute for the boundary electrophoresis and ultracentrifugal analysis.抄録全体を表示PDF形式でダウンロード (2102K) -
MUTUMI MURAMATU, TOSHIO ONISHI, SUMIKO MAKINO, SETSURO FUJII, YUICHI Y ...1965 年 57 巻 3 号 p. 402-406
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリーThe effect of various synthetic inhibitors derived from L-lysine and ε-ACA of caseino-lysis by plasmin was studied and compared with the effect of these substances on fibrin clot lysis. The L-lysine derivatives substituted in α-amino group (Nα-benzoyl-L-lysine etc.) and ε-ACA showed the slight enchancing effect on caseinolysis by plasmin rather than the inhibitory effect at low concentration, while these substances had a considerable inhibitory activity in fibrinolysis.
The derivatives substituted in the both α- and ε-amino groups of lysine (N, N'-dicarbo-benzoxy-L-lysine etc.) exhibited higher inhib-itory effect on caseinolysis, but lower activity than ε-aminocaproic acid in fibrinolysis. These facts might suggest that there is difference in the mechanism of inhibitory action by these substances between fibrinolysis and caseinolysis.
Among the synthetic inhibitors so far examined, the ester of ε-ACA had the extensive inhibitory effect on plasmin activity, and aminocaproic acid hexyl ester was the most remarkable inhibitor.抄録全体を表示PDF形式でダウンロード (387K) -
MAKOTO SEIJI, KIKUO OGATA1965 年 57 巻 3 号 p. 407-416
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリーThe incorporation of L-leucine-C14 by various cell particles of Harding Passey and B-16 mouse melanoma in vivo and in vitro was studied.
The incorporation rate of leucine into ribo-somes in vivo was fast at the early stage after injection and reached its maximum at the end of 12 minutes and decreased thereafter. Smooth membranes which are thought to be a pre-stage of melanosomes, showed relatively fast incorporation. The incorporation by melanosomes was very slow and the amount incorporated was also very low, therefore, it is very unlike that the protein biosynthesis in melanosomes de novo is so active.
Ribosomes isolated from melanoma synthesize in vitro the protein by the same mechanisms as liver ribosomes do.
The rough membranes had high incrporating activity which was markedly inhibited by puromycin. By contrast the activity is very low in the smooth membranes. Melano-somes and DOG-treated melanosomes have the incorporating activity which is partially inhibited by puromycin and KF. This sug-gests that the melanosomes may posses the protein biosynthetic system in its structure.
We want to thank Prof. Dr. H. Miyazaki, Juntendo University School of Medicine, and Prof. Dr. T. B. Fitzpatrick, Harvard Medical School, Boston, U.S.A. for their continuous advice and encouragement. And also their appreciation_??_s ex-pressed to Dr. H. Sugano, Department of Chemistray, Faculty of Science, Niigata University, for his co-operation with the ultracentrifugal studies. The early stage of this work was carried out at Department of Dermatology, Harvard Medical School, Boston, U.S.A. by the authors.抄録全体を表示PDF形式でダウンロード (1321K) -
The Effect of Detergents on the Conformation of Bacillus subtilis α-Amylase and Bence-Jones ProteinAKIRA IMANISHI, YOSHIHIDE MOMOTANI, TOSHIZO ISEMURA1965 年 57 巻 3 号 p. 417-429
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリーThe effects of three different kinds of detergents, namely, anionic, cationic and nonionic detergents, on the conformation of two different proteins were studied by the measurements of optical rotatory disperson and ultraviolet spectra and infrared spectra as functions of pH and concentration of the detergents. One of the protein examined is bacterial α-amylase which has a folded structure of single poly-peptide chain without disulfide linkage. The other one is Bence-Jones protein which may contain an intramolecular β-structure but not an α-helix. The experimental results revealed that change in optical rotatory dispersion by adding detergent was due to destruction of the original conformation followed by a partial α-helix formation of the unfolded poly-peptide chain, and that the extent of the α-helix formed depends upon the pH value. This refolded polypeptide chain was imbeded in a hydrophobic fabric produced by deter-gent associated around the polypeptide. An-ionic and cationic detergents act as a helix former even at extremely alkaline or acid pH region. On the other hand, nonionic detergent has not such an action throughout the pH region studied.
The authors wish to thank Dr. K. Hamaguchi for his helpfull discussions during this work, Dr. K. Fukushima for his valuable advices on the interpretation of infrared spectral data and also Mr. H. Matsuura for the measurements of infrared spectra. The authors are also indebted to Dr. S. Migita for the generous gift of Bence Jones protein which made this work possible and to Mr. H. Tokiwa (Kao Soap Co., Ltd.) for the gifts of detergents.抄録全体を表示PDF形式でダウンロード (1021K) -
YUZURU AKAMATSU, SHOSHICHI NOJIMA1965 年 57 巻 3 号 p. 430-439
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. Total phospholipids extracted with chloroform-methanol (2:1) from both BCG and M. plilei have been successfully separated by chromatography on a silicic acid impregnated paper and a thin-layer chromatoplate into at least five components.
2. They have been identified as cardio-lipin, phosphatidyl ethanolamine and phos-phatidyl-inositol oligo-mannosides by their Rf values, staining behaviors with specific reagents and the products of mild alkaline and acid hydrolyses. As for phosphatidyl-inositol oligo-mannosides, they have been found to be at least three different components which are separated each other as a phospholipid and the difference of which appears to be only their mannose contents.
3. From the results of quantitative analyses of individual phospholipids of M. phlei by thin-layer chromatography, it was demonstrated that about a half of total phospholipids was cardiolipin and about 40% of them was phosphatidyl-inositol oligo-mannosides on the basis of the phosphorus content. Only about 10% of total phospholipids was phosphatidyl ethanolamine. Several minor components found on a paperchromatogram after mild alkaline hydrolysis were very difficult to be determined whether they were genuine or secondary products.
4. Cardiolipins obtained from BCG and M. phlei have been revealed to have the same antigenic activity for “Wasserman Reaction” as that of ox heart.
The authors are indebted to Professor D. Mizuno, University of Tokyo, for his interest and helpful suggestions throughout this work. They also wish to thank Misses. N. Ninomiya and F. Suzuki for infra-red spectral measurements. They are grateful to the Ministry of Education and Takeda Pharmaceutical Industries, Ltd., for grants covering part of the expenses of the present investigation.抄録全体を表示PDF形式でダウンロード (1481K) -
VIII. Effect of Chromomycin A3 on the Nuclear RNA MetabolismYOSHIAKI MIURA, YOICHI MORIYAMA, SHUJI HASEGAWA, NORIKO FUKUI1965 年 57 巻 3 号 p. 440-444
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリーChromomycin A3 inhibits remarkably the metabolism of the cancer nuclear RNA. The specific inhibitory effect of the antibiotic was observed in the S-RNA synthesis, however, the end-turnover of S-RNa was not affected. The inhibitory action of Chromomycin was compared with that of Actinomycin and some difference in the action of the two antibiotics was discussed.抄録全体を表示PDF形式でダウンロード (388K) -
II. Role in Metabolic PathwayNOBUHIKO KATUNUMA, MITSUKO OKADA, TAKEO MATSUZAWA, YOSHIKATA OTSUKA1965 年 57 巻 3 号 p. 445-449
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリー1. It was found that glutamate was effectively produced from α-ketoglutarate sup-plied through TCA cycle by ornithine-ketoacid transaminase in the presence of ornithine.
2. The above reaction was sensitively inhibited by canalline.
3. The addition of ornithine caused the formation of 2 moles of glutamate and/or aspartate per each mole of ornithine comsumed in the mitochondrial system added acetate and succinate.
4. In the above system the employment of radioactive ornithine resulted in the formation of glutamate and aspartate having a hall specific activity compared with that of ornithine added.
5. Ornithine-ketoacid transaminase was sensitively induced with an increase of the dietary protein level in rats. The increase of the dietary protein from 20 to 60% casein resulted in 5 times increase in activity of the trans-aminase compared with that of the control animals.抄録全体を表示PDF形式でダウンロード (328K) -
MUTUMI MURAMATU, TOSHIO ONISHI, SUMIKO MAKINO, SETSURO FUJII, YUICHI Y ...1965 年 57 巻 3 号 p. 450-453
発行日: 1965/03/25
公開日: 2008/11/18
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HIDEMATSU HIRAI1965 年 57 巻 3 号 p. 454-456
発行日: 1965/03/25
公開日: 2008/11/18
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MAKOTO SEIJI, SADAO IWASHITA1965 年 57 巻 3 号 p. 457-459
発行日: 1965/03/25
公開日: 2008/11/18
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TATSUHISA YAMASHITA, YAEKO SOMA, SHIZUKO KOBAYASHI, TAKAMITSU SEKINE1965 年 57 巻 3 号 p. 460-461
発行日: 1965/03/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (176K)
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