巻号一覧
58 巻, 6 号
選択された号の論文の16件中1～16を表示しています
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I. Purification and PropertiesSADAMASA MINATO, TADASHI TAGAWA, KAZUO NAKANISHI1965 年 58 巻 6 号 p. 519-525
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリー1. Adenosine deaminase of Takadiastase was purified 340-fold by passage through a Duolite A-2 column, ethanol precipitation, and column chromatography on DEAE Sephadex A-50.
2. The enzyme has an optimal pH at 5.5. The enzymatic activity was inhibited by Ag+, Fe+++ and Hg++, but no ions with an activating affect were found. EDTA scarcely affected the enzymatic activity. The enzyme does not require metal ions.
3. The enzyme deaminates various adenosine derivatives and deoxyribose com-pounds, but NADP and NADPH2 are not deaminated. The nucleosides and nucleotides of guanine and cytosine are also refractory towards the enzyme action.
4. 2'-AMP and β-L-adenosine are de-aminated slowly, 9-β-D-glucopyranosyladenine is a substrate for the enzyme, but 9-α-pyranosyladenine is not.抄録全体を表示PDF形式でダウンロード (1127K) -
KIHACHIRO UEHARA, MASAO TAKEDA, YOSHITSUGU SAKATA1965 年 58 巻 6 号 p. 526-529
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーOsone reductase catalyzed the reduction _??_ of D-arabinosone to two pentoses. They were separated by column chromatography and converted into two hydrazone derivertives. One of them was identified as p-bromo-phenylhydrazone of D-ribose and the other p-nitrophenylhydrazone of D-xylose.抄録全体を表示PDF形式でダウンロード (297K) -
IV. Heterogeneity of Mitochondrial and Supernatant CatalaseTOKUHIKO HIGASHI, YASUO SHIBATA1965 年 58 巻 6 号 p. 530-537
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリー1. Rat liver mitochondrial fraction pre-pared with 10 per cent polyvinylpyrrolidone-8.5 per cent sucrose have been found to contain four catalases different in chromatographic behavior. Those catalases have purified and compared with each other.
2. Two out of the four catalases left the mitochondrial fraction to appear in the supernatant when sucrose alone was used as homogenizing medium.
3. The catalase appearing in the superna-tant fraction also showed a heterogeneous pattern on DEAE-cellulose chromatogram.
4. The heterogeneity of rat liver catalase
has been discussed in relation to the localiza-tion inside the cell and the metabolic sequence of this enzyme protein.
The authors wish to express their gratitude to Prof. N. Shimazono of the Department of Biochemi-stry, Faculty of Medicine, University of Tokyo, and Prof. H. Hirai of the Department of Biochemistry, School of Medicine, Hokkaido University, for their continuous encouragements during the course of this work. Their thanks are also due to Dr. T. Peters, JR., of the Mary Imogene Bassett Hospital, Cooper-stown, New York, for his valuable discussions and help in preparing the manuscript.抄録全体を表示PDF形式でダウンロード (1781K) -
IKUO YAMASHINA, KUNIHIKO IZUMI, HIROSHI OKAWA, EISUKE FURUYA1965 年 58 巻 6 号 p. 538-542
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリー1. For further studies of intracellular localization of hexosamine and sialic acid, the mitochondrial and the microsomal frac-tions of rabbit liver cells were submitted to purification and subfractionation. Mito-chondria was purified with particular atten-tion paid for microsomal contamination. Microsome was fractionated into smooth- and rough-surfaced microsomes.
2. It could be concluded that mitoch-ondria contained definite amounts of hexo-samine (1.7-2.0μg. per mg. protein) and sialic acid (1.3-1.6μg. per mg. protein).
3. Microsomal hexosamine and sialic acid were found to be concentrated in the smooth-surfaced microsome rather than in the rough-surfaced microsome.
4. The ratios of hexosamine: sialic acid in mitochondria and the smooth-surfaced microsome were similar for each other and also comparable to that found for nucleus.抄録全体を表示PDF形式でダウンロード (287K) -
I. Determination of the Glycolytic Intermediates in Human ErythrocytesSHIGEKI MINAKAMI, CHIYO SUZUKI, TAKAYASU SAITO, HARUHISA YOSHIKAWA1965 年 58 巻 6 号 p. 543-550
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリー1. The enzymatic methods were described for determining systematically the levels of glycolytic intermediates and nucleotides in the red blood cells, which were deproteinized immediately after removal from the body.
2. The contents of the metabolites in red blood cells of human males were found to be fairly constant, if the blood was subjected to analysis immediately after sampling from a vein and without further manipulation.
3. The sources of errors in the enzymic determination of glycolytic intermediates in red blood cells were discussed.抄録全体を表示PDF形式でダウンロード (543K) -
MOTOO HOZUMI, KUNIHIKO MATSUOKA, TAKASHI SUGIMURA1965 年 58 巻 6 号 p. 551-555
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリー1. Liver catalase activity of mouse was depressed progressively with time by the repeated administration of DDC, the effective doses being proportional to the body weight.
2. Repeated administration of DDC as well as AIA inhibited almost completely the recovery of the catalase activity after a single injection of AT.
3. The rate of destruction (KD) of the catalase activity lies around 0.020 with DDC, AT and AIA alike.
4. It is concluded that DDC inhibits the catalase synthesis in vivo just as AIA, although the intrisic mechanisms of inhibition may be different between them.抄録全体を表示PDF形式でダウンロード (312K) -
SHIGETAKE GANNO1965 年 58 巻 6 号 p. 556-560
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーThe physico-chemical and chemical pro-perties of the purified bacterial proteinase were reported. It was found that enzyme has a pH optimum for activity at 7 to 8.5, hydrolyzes casein optimally at 50° to 70°C, and is most stable at pH 6.5 to 10 and at the temperature below 35°C.
The electrophoretic mobility at pH 3.00 indicated the acidic nature of the enzyme protein and suggested the presence of a large number of free acidic groups in the enzyme molecule.
The enzyme was found to have a sedimenta-tion constant of 1.58 S from which its molecular weight was calulated to be 27, 000±2, 500.
The amino acid composition of the enzyme protein showed that it contained no cystine residues. The enzyme was not in-activated by the addition of thiol reagents, indicating that it is a non-sulf hydryl enzyme.
The author wishes to thank Prof. H. Hatano of Kyoto University for his helpful discussion and sug-gestions during this work and Dr. K. Sumizu and other members of Kyoto College of Liberal Arts for technical assistance on amino acid analysis.抄録全体を表示PDF形式でダウンロード (328K) -
XV. Cytochrome Oxidase Activity of the Okunuki Preparation and Its Activation by Heat, Alkali and Detergent TreatmentsYUTAKA ORII, KAZUO OKUNUKI1965 年 58 巻 6 号 p. 561-568
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーThe cytochrome oxidase activity of our preparation was investigated and found to be similar to those of preparations obtained in several other laboratories for which different purification procedures were used.
The pH optimum was at 5.62 in McIlvaine's buffer at 0.04M with respect to phosphate, and the maximum specific activity was 5.64 sec.-1/mg. protein/cuvette. Using a Na2HPO4-KH2PO4 buffer system, the highest activity was found at 0.075M at pH 5.95.
The activity was increased by treating cytochrome a with heat, alkali or anionic detergents. Guanidine hydrochloride had the greatest effect, increasing the specific activity to 17.1.
The relationship between the physical state of cytochrome a and its activity was discussed.抄録全体を表示PDF形式でダウンロード (524K) -
KOICHI KIMURA, TETSU ONO, TOMOMICHI YANAGITA1965 年 58 巻 6 号 p. 569-576
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーRibosome monomers were isolated from dormant and germinated conidia of Aspergillus oryzae. These ribosomes obtained at different developmental stages showed essentially the same chemical and physicochemical character-istics, indicating that the dormancy of conidia is not a state involving a modification of ribosome monomers.
A. oryzae conidia ribosomes contain 44 per cent protein and 56 per cent RNA and have a sedimentation coefficient of 80 S at 20°C on infinite dilution. These particles are stable at pH 7.6 in the presence of 10mM Mg++ and undergo a dissociation into 60 S and 39 S subunits at lower concentrations of Mg++. A prolonged dialysis of the particles against 0.1mM Mg++ caused a conversion of 60 S subunit into 51 S particles. The base composition of ribosomal RNA was analyzed. The presence of latent ribonuclease in the ribosomes was also demonstrated.
We are indepted to Dr. K. Miura, Nagoya University, for his kind assistance in nucleotide analysis. This work was aided in part by a grant from the Scientific Research Funds of the Ministry of Education.抄録全体を表示PDF形式でダウンロード (1832K) -
TOMONOBU TOKIWA, TAKAMICHI SHIMADA, YUJI TONOMURA1965 年 58 巻 6 号 p. 577-581
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーThe time-course of the change in H+ concentration during actin polymerization was measured with a pH meter and a titri-graph. The pH-dependence of the amount of H+-liberation during the polymerization agreed well with that of H+-liberation on hydrolysis of ATP by myosin. Between pH 8 and 9, 1 mole of H+ liberated per 57, 000g. of actin, but the liberation decreased at below pH 8. However, the time-course of H+-libera-tion during the polymerization was not parallel with the increase in viscosity or P1-liberation which was measured after stopping the reac-tion by adding perchloroacetic acid; H+-liberation occurred after a lag phase, while P1-liberation and increase in viscosity occurred as a l st order reaction, without any lag phase.抄録全体を表示PDF形式でダウンロード (339K) -
HIROSHI NAKAMURA, TOHRU MORI, YUJI TONOMURA1965 年 58 巻 6 号 p. 582-586
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーMyofibrillar fragments were obtained from chicken pectoral muscle, rabbit leg red muscle and psoas white musle. The contrac-tion of myofibrillar fragments and their ATPase activites were measured in 0.04M KC1-20μM MgCl2 at pH 7.0. The following results were obtained.
1. Pi-liberation in the initial phase of myofibrillar ATPase was measured but it seemed to be linear with time. The average ATPase acivities at 15°C of preparations from chicken pectoral muscle, preparations from rabbit leg red muscle and preparations from rabbit psoas white muscle were 98, 107 and 84μmoles P1/g./minute, respectively.
2. It was found that the length of sarco-meres of chicken pectoral muscle and rabbit
psoas muscle shortened from the original length of 1.8 to 1.0μ at the stage of forma-tion of contraction bands, and that myofibril-lar fragments from rabbit psoas muscle and leg red muscle were considerably homogeneous in their ability to undergo ATP-induced shortening.
3. The amounts of ATP-splitting of myofibrillar fragments from chicken pectoral muscle, rabbit leg red muscle and rabbit psoas muscle necessary to shorten to the stage of disappearance of the I-bands were 6.8, 425 and 2.7μmoles ATP-split/g. protein, respectively.抄録全体を表示PDF形式でダウンロード (345K) -
YOKO TSUSUÉ, TAMIO YAMAKAWA1965 年 58 巻 6 号 p. 587-594
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーThe chemical structure of oligosaccharides isolated from the blue-green alga, Tolypothrix tenuis, was examined by gas-liquid partition chromatography and enzymatic hydrolysis.
After the methylation of the sample with methyl iodide catalyzed by methylsulfinyl carbanion, products were subjected to metha-nolysis. The gas chromatogram of a group of methyl methylated hexosides thus derived from T. tennis material was compared with those derived from several saccharides, such as sucrose, melezitose, planteose, inulin and Jerusalem artichoke oligosaccharides.
From the results of gas chromatography together with those of enzymatic hydrolysis employing α- and β-glucosidases and p-fructo-furanosidase, a formula of fructofuranosyl-(2→4)n-fructofuranosyl-(2→1)-α-glucopyranoside is tentatively presented for the T. tennis oligo-saccharides.
The junior author (Y. T.) expresses her heartfelt gratitude to Prof. T. Yanagita of Institute of Applied Microbiology, University of Tokyo, for his valuable suggestions during the course of this investigation and in the preparation of this manuscript, and to Prof. K. Hogetsu of Tokyo Metropolitan University for his encouragement throughout. Thanks are also due to Dr. A. Makita of Tohoku University and to Dr. H. Suzuki of Tokyo Kyosiu Universiyt for their valuable discussions.抄録全体を表示PDF形式でダウンロード (575K) -
KEIKO IKEDA, MOTOO HOZUMI, TAKASHI SUGIMURA1965 年 58 巻 6 号 p. 595-598
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーCytochrome b5 and P-450 in microsomes, and catalase were analysed in the liver of normal and rats bearing Rhodamine sarcoma, Methyleholanthrene sarcoma, ascites hepatomas AH 130 or AH 66 F.
In the liver of all animals bearing any one of tumors, the liver catalase activity was depressed. Significant decreases in cytochrome b5 and P-450 level were observed in the liver bearing AH 66F or methylcholanthrene sarcoma.
This is the first case of the depression of hemoprotein in the liver of tumor-bearing animals other than catalase.
The authors gratefully acknowledge the interest of Dr. W. Nakahara, the director of this institute, in this work.抄録全体を表示PDF形式でダウンロード (284K) -
MASACHIKA IRIE1965 年 58 巻 6 号 p. 599-603
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (300K) -
HAJIME SHIGEMATSU, HIRO-O TAKESHITA, SHIZUE ONODERA1965 年 58 巻 6 号 p. 604-606
発行日: 1965/12/25
公開日: 2008/11/18
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YUMIKO OHTA, YASUYUKI OGURA1965 年 58 巻 6 号 p. 607-608
発行日: 1965/12/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (103K)
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