巻号一覧
59 巻, 3 号
選択された号の論文の19件中1～19を表示しています
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IV. Relationship between Rapidly Labelled RNA and Induced-Synthesis of Tryptophan Oxygenase in VivoKATSURO KOIKE, TADAHIKO OTAKA, SEIICHI OKUI1966 年 59 巻 3 号 p. 201-208
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. The microsomal RNA from mouse liver labelled with P32-phosphate in vivo was analyzed by sucrose-density-gradient centrifu-gation. After labelling with P32-phosphate for 60 and 120 minutes the isotope distri-bution in the microsomal RNA prepared in the presence of 0.01M magnesium sulfate is obviously heterogeneous, components possessing sedimentation rates of 4S to 29S, although most of the transfer RNA (4S) had been eliminated. After labelling for 60 minutes, the radioactive RNA in the 4S to 6S regions had a high specific activity and the base ratio measured by counting the four nucleoside monophosphates did not show the end-turnover of transfer RNA. It is clear that the rapidly labelled RNA exists in micro-some fractions which contain polysomes.
2. In the initial phase of substrate in-duction, no new synthesis of rapidly-labelled RNA was detected in the cytoplasm by sucrose-density-gradient centrifugation. In the fully induced-phase, the rapid breakdown of a certain fraction of rapidly labelled RNA is coupled with an actual increase in the total amount of enzyme protein (10). It seems likely that the template RNA for the enzyme protein is already present before the inducer acts.
3. Like the effect of inducer on the rapidly labelled RNA, in the initial phase and fully-induced phase of substrate induction, the rapid appearance and disappearance of the p-region of polysomes are coupled with enzyme protein synthesis in vivo. After sub-strate induction, the enzyme activity returns to the normal value and the mass distribution of polysomes also returns to almost normal.
4. Cortisone treatment does not result in the disappearance of the p-region in the polysomes distribution in contrast with sub-strate induction causes a significant increase in the distribution of a polysomes of larger size than in the d-region because of the direct intervention of the regulatory gene.抄録全体を表示PDF形式でダウンロード (658K) -
Effect of Some Aromatic Compounds on the Level of Glutathione and the Activity of Glutathionase in the RatTETSUYA SUGA, ISAO OHATA, MASUO AKAGI1966 年 59 巻 3 号 p. 209-215
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. Six hours after the administration to the rat of several compounds known to be excreted as mercapturic acids, glutathione level in the liver fell markedly but that in the blood was not significantly affected.
2. No significant change in glutathionase activity was observed in the kidney of rats dosed with aromatic compounds.
3. On repeated administration of bromo-benzene to the rat, glutathione levels in the liver and blood were found to increase. On the other hand, glutathionase activity in the kidney and excretion of mercapturic acid were found to decrease.抄録全体を表示PDF形式でダウンロード (460K) -
RYUZO SADAHIRO, YOSHIKAZU HINOHARA, AKIHIRO YAMAMOTO, MINORU KAWADA1966 年 59 巻 3 号 p. 216-222
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. D-Glucuronolactone dehydrogenase was purified about 10-fold from guinea pig liver by ammonium sulfate fractionation and DEAE-cellulose column chromatography.
2. The activity ratio (GL/ML) during the purification steps was constant, no difference was observed between the activities on GL and ML in heat stability and the competitive inhibition was observed in the test of the combined substrate system. These results suggest that a single enzyme may be responsible for the dehydrogenation of GL and ML.
3. This dehydrogenase was found to be an SH-enzyme.
4. From the experimental results using cyanide as a trapping agent to give cyanohydrin between free aldehyde group and cyanide, it is suggested that the aldehyde form of GL and ML may act as direct precursors in their enzymatic dehydrogenations.
5. Some considerations on the inhibitory effect of an initial product derived from GL on β-glucuronidase were discussed from the above observations.
The authors wish to express their gratitude to Dr. M. Ishidate, Prof. Emeritus of the University of Tokyo, the Director of the Tokyo Biochemical Research Institute, Dr. M. Okada, Chief of the Tokyo Bio-chemical Research Institute for their valuable advices, and to Dr. T. Nanbara, Assoc. Prof. of the University of Tokyo, Dr. M. Kawata, Assoc. Prof, of Hokkaido University for their useful suggestion in polarographic studies. The authors are also indebted to Dr. T. Akiba, Prof. Emeritus of the University of Tokyo, the Director of the research laboratories, Dr. Y. Nitta, the head of this department for their continued encouragement and guidance through this work.抄録全体を表示PDF形式でダウンロード (511K) -
HAJIME SHIGEMATSU, HIRO-O TAKESHITA1966 年 59 巻 3 号 p. 223-229
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリーAntisera were prepared by immunization of rabbits with fibroins, one of which was isolated from silkglands of larvae in the fifth instar and the other from cocoons. The serological characters of these were studied. The antisera were specific for the fibroins and gave quantitative precipitin reactions. The precipitin curve obtained was used for determination of the fibroin in subcellular fractions of the posterior silkgland. It was found that fibroin was mostly present in large and small microsomes. The fibroin of the former was thought to be derived from the endoplasmic reticulum and that of the latter to be bound to an equimolar amount of ribosomal RNA.抄録全体を表示PDF形式でダウンロード (448K) -
Estimation of Nucleosides Present at the 5'-Phosphate Terminus of a Limit Digest of DNA by DNase IMAKOTO MATSUDA, HEIHACHIRO OGOSHI1966 年 59 巻 3 号 p. 230-235
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリーTo study the specificity of deoxyribonuclease I (DNase I), the nucleosides at the 5'-phos-phate termini of a DNase I limit digest were analyzed. The results obtained suggest that DNase I mainly cleaves the phosphodiester linkage (-p-X_??_p-Py-p-) adjacent to a pyrimidine nucleotide, rather than a purine nucleotide.
The authors wish to thank Prof. K. Makino for his encouragement, and Prof. T. Minami for generously supplying the human prostate glands used in the present investigation.抄録全体を表示PDF形式でダウンロード (391K) -
YOSHIHITO YAOI1966 年 59 巻 3 号 p. 236-246
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリーThe peptides from the chymotryptic digest of baker's yeast (Saccharomyces oviformis) cytochrome c were fractionated by chromato-graphy on a column of Dowex 50-X2. When further fractionation or purification was required, the heterogeneous fraction was submitted to paper chromatography, paper electrophoresis or column chromatography with Dowex 50-X2, Dowex l-X2, DEAE-cel-lulose, CM-Sephadex A-50 or Sephadex G-25 in order to obtain homogenous peptides.
By the various combination of these fractionation methods, eighteen peptides were isolated in molar yields higher than 10% and fourteen peptides in the lower yields. Amino acid compositions of the purified peptides showed that they were pure enough to permit the subsequent study on their amino acid sequences.
The author is gratefull to Prof. K. Narita and Dr. K. Titani for their guidances and encouragements during the course of the present study. Thanks are also due to Mr. H. Murakami for the skillful performance of amino acid analyses by an automatic machine.抄録全体を表示PDF形式でダウンロード (694K) -
YOSHIHITO YAOI, KOITI TITANI, KOZO NARITA1966 年 59 巻 3 号 p. 247-256
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリーIn order to arrange the thirteen tryptic peptide fragments of cytochrome c from baker's yeast (Saccharomyces oviformis) (3) into the complete primary structure, the amino acid sequences of the chymotryptic peptides, which seemed to be bridges between the tryptic fragments, were determined. The tryptic peptide fragments could be combined into six. Two out of six fragments were the N- and C-terminal ones, respectively, and the N-terminal groups of all of the remaining four were lysine. Therefore the attempt to complete the primary structure was unsuccessful.
The authors are gratefull to Mr. H. Murakami for the performance of amino acid analyses with a Beckman/Spinco automatic equipment.抄録全体を表示PDF形式でダウンロード (613K) -
MASAHIKO KODAMA, YUSAKU TAGASHIRA, AKIRA IMAMURA, CHIKAYOSHI NAGATA1966 年 59 巻 3 号 p. 257-264
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. The solubility of 3, 4-benzopyrene and pyrene in DNA solution has been investigated in several DNA and NaCl concentrations, and an attempt has been made to explain the discrepancies between the results of Boy1and and those of Liquori. Under salt free conditions, double-stranded native DNA solubilizes more aromatic hydrocarbons than single-stranded, heat-denatured DNA, as was observed by Boy1an d. However, by the addition of salt, their interrelationship can be revers-ed, as was asserted by Liquori.
2. One to one correspondence has been observed between spectral shift of maximum peak of pyrene and secondary structure of DNA, which was confirmed by several methods of denaturation of DNA (heat, dilution, acid, alkali and urea denaturation).
3. Some attempts have been made to clarify the mode of interaction between aromatic hydrocarbons and DNA. In accord with the results of flow-dichroism, these ob-servations seem to support the intercalation-model.
We are grateful to Dr. R. W. Person, Jr., Merck Sharp and Dohme, Inc. for generous supply of actinomycin D.抄録全体を表示PDF形式でダウンロード (523K) -
II. Studies on the Structure of the SubstratesSADAMASA MINATO, TADASHI TAGAWA, MICHIKO MIYAKI, BUNJI SHIMIZU, KAZUO ...1966 年 59 巻 3 号 p. 265-271
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリーAdenosine deaminase from Takadiastase deaminated CoA, 2', 3'-cyclic AMP and 3', 5'-cyclic AMP, although the rate of deamination of 2', 3-cyclic AMP was very slow. 9-sub-stituted adenine compounds were deaminated, but 3-substituted compounds was not. August-mycin C and Tubercidin were not deaminated. The Km values of these substrates were very similar. The structure required for the substrates to be deaminated was discussed.
The authors wish to thank Dr. N. Otaka for his kind gift of Angustmycin C and Dr. S. Shirato for his kind gift of Tubercidin. Thanks are also due to Mr. K. Terui for his assistance in the experimental work.抄録全体を表示PDF形式でダウンロード (1836K) -
TOKUTARO HISHIZAWA, HIROSHI TERAYAMA1966 年 59 巻 3 号 p. 272-279
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. The cell-free amino acid incorporating systems from rat liver using either microsome or ribosome as well as cell sap fractions have been studied to elucidate the mechanism for the increased protein synthesis of regenerating liver. The amino acid incorporating activity of both microsomes and ribosomes were greatly damaged by the preincubation (37°C, 30min.). The activity of ribosomes, however, was re-stored by the addition of external messenger RNA (poly U), but the activity of preincubated microsomes was not. It was concluded that amino acid incorporating activity of ribosomes depends upon the content of mes-senger RNAs attached to the particles.
2. By comparing the amino acid incor-porating activities of the cross-combination systems, such as Rr+Sr, Rr+Sc, Rc+Sr and Rc+Sc, information on the relative activity of either ribosome or cell sap components from regenerating liver compared with normal liver has been obtained. The results showed that the activity of the regenerating liver ribosomes started to increase after a lag period of about 5 hours, reaching a very broad maximal level at 30 hours, followed by a gradual decrease; the cell sap activity started to increase after a lag period of about 15 hours, reached a maximal at 30 hours, and then decreased rather rapidly. Since the amino acid incorporating activity of the ribosomes depends upon the messenger RNA content, the above observation is considered to show the enrichment of messenger RNA in the cytoplasmic ribosomes during the first 5-30 hours after partial hepatectomy.
The authors wish to express their hearth thanks to Prof. T. Miyakawa, University of Tokyo, for his constant encouragement throughout the present study.抄録全体を表示PDF形式でダウンロード (600K) -
IV. Liberation of ADP from the Myosin-ATP System and Effects of Modifiers on the Phosphorylation of MyosinKIICHI IMAMURA, MICHIHIKO TADA, YUJI TONOMURA1966 年 59 巻 3 号 p. 280-289
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリーAn initial burst of Pi-liberation was observed not only from the myosin-ATP system but also from the cardiac myosin B-, the skeletal H-meromyosin-, and the skeletal subfragment S1-ATP systems. The amounts of the initial bursts from these systems were always almost one mole per mole of protein.
The time-course of ADP-liberation was followed by measuring the oxidation of NADH in the myosin-ATP system coupled with pyruvate kinase and lactate dehydrogenase. Liberation of ADP did not show an initial burst. The dependence of the rate of oxidation of NADH on the ATP concentration showed that myosin contains one mole of ATP-binding site per 4×105g. and in the steady state it contains no bound ADP.
The initial rapid liberation of Pi was not affected by DNP or G-strophanthin. The dependences of the reciprocals of the amounts of the initial bursts from the myosin-, and the H-meromyosin-ATP systems on various divalent cations were very similar to those of the rates of the O18-exchange reaction catalyzed by these two proteins, reported by Koshland et al.抄録全体を表示PDF形式でダウンロード (691K) -
IV. Calorimetric Determination of α-1, 4 Glucosidic Linkage Content in Some Starches and GlycogensKATSUTADA TAKAHASHI, SÔZABURO ONO1966 年 59 巻 3 号 p. 290-294
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. Calorimetric studies on the hydrolytic reactions of starches from various sources and of animal- and phytoglycogens catalyzed by glue-amylase of Rh. Delemar have been made.
2. By the use of calorimetric data found for the α-1, 4 and α-1, 6, glucosidic linkages (1-3), α-1, 4 glucosidic linkage content in the respective sample has been determined.
3. Combining the above data with those concerning the amylose content of the starches determined by the amperometric titration method, the average length of unit chain of the amylopectin component has been calculated.
4. The average length of unit chain of the glycogens has also been calculated.
The authors are indebted to Dr. S. Suzuki and Dr. S. Shibata, both of Food Research Institute, Ministry of Agriculture and Forestry and to Prof. Z. Nikuni and Dr. S. Hizukuri, both of Osaka University for supplying the samples.抄録全体を表示PDF形式でダウンロード (332K) -
KAZUO NAGAI, FUTOSHI IZUMI, HIROSHI YOSHIDA1966 年 59 巻 3 号 p. 295-303
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. The subcellular distributions of K+-dependent phosphatase and of Na+, K+-dependent ATPase were investigated in brain, kidney and liver, and it was observed that the distribution of K+-dependent phosphatase was observed that the distribution of K+-dependent phosphatase was similar to that of Na+, K+-dependent ATPase.
2. The specific activities of the K+ dependent phosphatase and of Na+, K+-dependent ATPase were increased by treatment with NaI and both activities were decreased by pretreatment with protamine, PCMB, igrosin or acetone.
3. The properties of the K+-dependent phosphatase in a brain preparation pretreated with NaI were examined. It was found that the optimum pH was around 8.0, and the maximum activity was obtained in the presence of 5mM MgCl2 and 30mM KCl with 5mM of p-nitrophenyl phosphate as substrate. However, the optimum concentration of K+ varied with the Mg concentration in the reaction mixture.
4. K ion could be effectively replaced by Rb+, NH4+ and Cs+, but Li+ and Na+ had almost no effect. However, when a large amount of enzyme was used, a significant stimulation by Na+ was observed.
5. Ouabain, Ca++, Na+, ATP, ADP and inorganic orthophosphate inhibited the K+-dependent phosphatase activity. Ouabain was competitive with K+, Ca++ was com-petitive with Mg++, and ATP, ADP and Pi were competitive with the substrate. More-over, it was found that the inhibitory effect of ATP was also dependent on the potassium concentration. Na+ showed a stimulatory effect on the K+-dependent phosphatase in the presence of a low K+ concentration and inhibitory effect when the medium contained a sufficient amonut of K+. Furthermore, it was found that the inhibitory effect of Na+ was related with the substrate concentration.抄録全体を表示PDF形式でダウンロード (518K) -
III. Formation of Glycine from C14-Glycolic Acid and the Occurrence of Glycolate OxidaseMICHIKO MURAMATSU, KOHEI OTOMO, KENSUKE SHIMURA1966 年 59 巻 3 号 p. 304-309
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリー1. The conversion of glycolate to glycine was demonstrated in the silkworm by an in vivo experiment with C14-glycolate. A less but significant conversion of C14-glycolate to serine and alanine was also observed.
2. Glycolate oxidase activity was found in the homogenate of fatty body of silkworms. Other tissues such as silkglands and digestive tract showed no detectable activity.
3. This enzyme activity was shown to be stimulated about two-fold by 5×10-4M of hydroxylamine. A plausible explanation for the stimulation was presented.
Authors wish to express our deep appreciation to Dr. T. Kurasawa, the chief of the Experimental Sericultural Station of Miyagi Prefecture, for his kind supply of the silkworm.抄録全体を表示PDF形式でダウンロード (397K) -
KOSCAK MARUYAMA, YAYOI ISHIKAWA-KATSUKI1966 年 59 巻 3 号 p. 310-312
発行日: 1966/03/25
公開日: 2008/11/18
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KUNIO YAGI, TOSHIHARU NAGATSU, IKUKO NAGATSU-ISHIBASHI, AKIRA OHASHI1966 年 59 巻 3 号 p. 313-315
発行日: 1966/03/25
公開日: 2008/11/18
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MIHOKO YOSHIDA, SHIGEO HORIE, NORIO SHIMAZONO1966 年 59 巻 3 号 p. 316-319
発行日: 1966/03/25
公開日: 2008/11/18
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JUN OKUDA, KENTARO OKAMURA, KUNIO YAGI1966 年 59 巻 3 号 p. 320-321
発行日: 1966/03/25
公開日: 2008/11/18
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YUJI TONOMURA, TOMONOBU TOKIWA, TAKAMICHI SHIMADA1966 年 59 巻 3 号 p. 322-324
発行日: 1966/03/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (206K)
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