巻号一覧
60 巻, 2 号
選択された号の論文の21件中1～21を表示しています
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VI. Molecular Weight and Some PropertiesEIICHI MISAKA1966 年 60 巻 2 号 p. 103-107
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーThe molecular weight of lipoamide dehydrogenase from Saccharomyces oviformis is about 100, 000 to 110, 000 as calculated from the results of ultracentrifugal and diffusion studies. The value of S0, 20, w is 5.96S and D0, 20, w, is 5.25×10-7 cm2./sec. The enzyme has two moles of FAD per mole and is divided into smaller fragments on reduction in the presence of PCMB and 4.5 M urea. These fragments seem to have the same molecular weight and are combined with each other through the catalytic disulfide linkage in the native enzyme.
The author wishes to thank Dr. G. Sunagawa, the director of the Laboratories, for encouragement during this investigation and Dr. K. Nakanishi, for valuable advice and discussion.抄録全体を表示PDF形式でダウンロード (1054K) -
II. Some Physical Properties of the Labelled Myosin AMINORU KASUYA, HIDEO TAKASHINA1966 年 60 巻 2 号 p. 108-114
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーSome physico-chemical studies were carried out on myosin A labelled with a fluo-rescent dye, 1-dimethylaminonaphthalene-5-sulfonyl chloride, and the following results were obtained.
1. The appearance of the faster sediment-ing peak and the increase in intrinsic viscosity became more remarkable, as the degree of the labelling with the dye increased, whereas considerable changes in the content of abnormal tyrosine and in the helical content were scarcely observed.
2. The attached dye was scarcely released by dialysis against alkaline solutions and the ATPase activity of myosin A was not restored by this treatment.
3. The dye-labelled myosin A emitted a visible fluorescence with a maximum at 505 mμ when the dye moiety was excited at 330 mge, and it emitted both the ultraviolet fluorescence of 340 mμ and the visible fluo-rescence of 505 mμ when the aromatic residues of the protein was excited at 285 mμ. Urea or dioxane decreased both the fluorescence intensity and -[m']400. Ethanol scarcely influenced the helical content estimated from the bo term, but increased the ultraviolet fluorescence and decreased the visible fluo-rescence.
Based on these results, some physical properties of the dye-labelled myosin A and the structure of the part attached by the dye were discussed.
The authors would like to thank Departments of Physics and Pharmacology in Sapporo Medical College for the instrumentation for the measurements of optical rotatory dispersion and fluorescence spectra.抄録全体を表示PDF形式でダウンロード (1092K) -
TSUNEKO UCHIDA1966 年 60 巻 2 号 p. 115-132
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリー1. A method for the purification of RNase T2 [EC 2. 7. 7.17] is described, which consists of water extraction, batch-wise treatment with DEAE-cellulose, heat treatment, concentration with ammonium sulfate, DEAE-cellulose column chromatography, ethanol fractionation and DEAE-cellulose column chromatography with a milder elution gradient system.
2. By this procedure, RNase T2 was purified 900-1100-fold with a yield of about 10% (15-20 mg. with combined RNase T2-A and RNase T2-B) from 500g. of Taka-Diastase powder.
3. The purified RNase T2 was demon-strated to be homogeneous as a protein in chromatography on DEAE-cellulose, gel filtra-tion on Sephadex G-75, paper electrophoresis, sedimentation and N-terminal amino acid analysis. Furthermore, this preparation was obtained free from various contaminating enzymes in Taka-Diastase.
4. Molecular weight of RNase T2 was estimated to be 36, 000 from sedimentation equilibrium analysis and minimum molecular weight calculated from the analytical results of amino acid composition.
5. The isoelectric point was found to be around pH 5.
6. The N-terminal amino acid was determined as glutamine or glutamic acid.
7. The amino acid composition of RNase T2 was determined. This enzyme contains 6 residues of histidine, 7 residues of tryptophan and one residue of methionine.
8. Some neutral sugars were contained in the purified RNase T2.
9. Some enzymatic properties of RNase T2, such as stability, pH optimum and sensitivities toward inhibitors and activators, were investigated in detail.
10. RNase T2 was separated into RNase T2-A and RNase T2-B by the DEAE-cellulose column chromatography. RNase T2-A was indistinguishable from RNase T2-B in the enzymatic properties and the properties as a protein. The difference between the both fractions was observed only in their carbo-hydrate components.
This work has been supported by a grant to Prof. F. Egami from the Toyo Rayon Science Foundation, to which the author's thanks are due. The present author wishes to express her sincere thanks to Prof. F. Egami for his guidance and encouragement during this work.抄録全体を表示PDF形式でダウンロード (2567K) -
Participation of Glutathionase in the Conversion of Glutathione Derivatives to Cysteine DerivativesTETSUYA SUGA, HIROSHI KUMAOKA, MASUO AKAGI1966 年 60 巻 2 号 p. 133-139
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーS-Benzylglutathione (BzSG) and some other glutathione derivatives were found to be converted into cysteine derivatives by rat-kidney microsomes and to serve as com-petitive inhibitors to glutathionase. These conversions were similar to the metabolic breakdown of glutathione by the gluta-thionase system.
Glutathionase was solubilized by treat-ment with deoxycholate or with heat-treated snake venom and the activity to break-down glutathione derivatives was also solubilized in parallel with the glutathionase activity.
Evidence was presented that the break-down of glutathione derivatives was cata-lyzed by the glutathionase of rat-kidney microsomes, and this suggests that gluta-thionase participates in the formation of mercapturic acid in animals.
The authors thank Yamanouchi Pharmaceutical Co., Ltd., Tokyo for a kind gift of reagents.抄録全体を表示PDF形式でダウンロード (528K) -
HAJIME SHIGEMATSU, HIRO-O TAKESHITA, SHIZUE ONODERA1966 年 60 巻 2 号 p. 140-146
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーPosterior silkglands of the silkworm were collected from larvae on the 4 th to 6 th day of the fifth instar and after incubation in vitro with glycine-C14, incorporation of radioactivity into fibroin in subcellular fractions was studied. The greatest and most rapid incor-poration was observed in the supernatant (105 S) after centrifugation at 105, 000×g for 60 minutes. After DOC treatment, the soluble part (M-S) of fraction M (precipitate after centrifugation at 105, 000×g for 60 minutes) showed an incorporation curve similar to that of 105S, but the incorporation to the DOC-insoluble part (M-P) of fraction M was less and similar to that of the DOC-soluble part (LM-S) of fraction LM (precipitate after centrifugation at 20, 000×g for 30 minutes). The rate of labelling of fibroin in the insoluble fraction (LM-P) after treatment of fraction LM with DOC was, as it was also the case with fraction CD, almost linear during the reaction period.
After 15 minutes' incubation, the specific radioactivity of fibroin in fraction R was much higher than that in fraction S, which was obtained by further centrifugation of fraction 105S for 180 minutes at 105, 000×g.
Sucrose density-gradient sedimentation analysis of fractions R and M indicated that fraction R mainly consisted of free ribosomes, while fraction M contained large, hetero-geneous particles. In fraction R, the radio-activity was found in the ribosomal peak. There was unexpectedly low labelling of fraction M.
The effect of ribosomes being in a free state on fibroin synthesis was discussed.抄録全体を表示PDF形式でダウンロード (449K) -
MICHIHIRO SUGANO, ISAO CHINEN, MASAFUTO WADA1966 年 60 巻 2 号 p. 147-153
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーThe rate and fatty acid specificity of cholesterol esterification by the blood serum of laying hens was studied in vitro.
In the laying hens, levels of free cho-lesterol in the serum and in the β-lipoprotein fraction were considerably higher, and the ratio of ester to total consequently lower, than those for the male or non-laying female birds. The latter difference in both the male and non-laying females was from 68-72% to 26-49%. The values for a-lipo-proteins, however was essentially the same in both sexes.
The fatty acid composition of cholesterol esters of laying hen serum was characterized by higher levels of saturated and monoenoic acids and lower levels of the polyunsaturated acids, as compared with the corresponding values for the male and non-laying female. These trends were especially clear in the cholesterol esters of the β-lipoprotein fraction.
The rate of cholesterol esterification by layers' whole serum in vitro was substantially slower than that of the male, the results being comparable to those obtained by in vivo experiments. However, cholesterol esteri-fying activity in serum α-lipoproteins of the laying hen was significantly higher than that in the β-lipoprotein fraction. The specific activity of newly formed cholesterol esters was again found to be heterogeneous with respect to the different fatty acid esters, as is true of the male and non-laying female. Polyunsaturated acid esters showed a high activity and saturated esters low activity -compared to that of total cholesterol esters. Although the enzyme obtained from laying hens was markedly less active in regard to saturated acids, that from the β-lipoprotein fraction did appear to have a greater esteri-fying potential for these acid esters than did the enzyme in the α-lipoprotein fraction.
Some estimation of the fatty acid speci-ficity of the serum enzyme was made. Results indicate that the sera from both male and laying female birds generally have the same specificity, but that the influence of the fatty acid used as donor must not be overlooked.抄録全体を表示PDF形式でダウンロード (532K) -
HIROMICHI OKUDA, SETSURO FUJII1966 年 60 巻 2 号 p. 154-159
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーRat epididyrnal adipose tissue contains a lipase [EC 3. 1. 1. 3] and an esterase [EC 3. 1. 1. 1]. Esters of long chain fatty acids are preferentially hydrolyzed by the lipase. On the other hand, esters of short chain fatty acids are preferentially hydrolyzed by the esterase. Both alcohol moiety of esters and position of glycerides are not factors to differentiate between the lipase and the esterase. Purification of these lipase and esterase is also reported.抄録全体を表示PDF形式でダウンロード (440K) -
SHUZO YAMAGATA, KIYOSHI UEDA, RYO SATO1966 年 60 巻 2 号 p. 160-171
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリー1. Calf thymus nuclei isolated in 0.25M sucrose-3mM CaCl2 were purified by density gradient centrifugation and Triton X-100 treatment. The nuclei thus obtained seem to be rather pure, judging from their chemical composition and the activities of mitochondrial and microsomal marker enzymes.
2. Spectrophotometric studies revealed that the purified nuclei contained cytochromes of the b and c type at concentrations not explicable as due to contamination with mitochondria and microsomes.
3. Further evidence was provided that the c-type cytochrome in isolated nuclei is not mitochondrial in origin.
4. Nuclear cytochromes could be reduced with NADH and NADPH, suggesting that a respiratory chain involving these cytochromes is functioning in calf thymus nuclei.
The authors are indebted to Sankyo Company for their kind gift of yeast cytochrome c.抄録全体を表示PDF形式でダウンロード (899K) -
HIROKO NISHIBAYASHI, TSUNEO OMURA, RYO SATO1966 年 60 巻 2 号 p. 172-183
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーThe slow, cyanide-insensitive oxidation of NADPH by oxygen catalyzed by rabbit liver microsomes is greatly stimulated by K3, 1, 4- or 1, 2-naphthoquinone. The K3-stimu-lated oxidation, but not the slow oxidation in the absence of K3, is considerably activated by high phosphate buffer concentrations. The K3 concentration giving half maximal stimu-lation of NADPH oxidation is 3.2 μM. The NADPH oxidation in the presence of K3 is accompanied by oxygen consumption; the reduction product of oxygen seems to be mainly hydrogen peroxide. Although it appears likely that in this oxidation K3 functions as a catalyst by undergoing reduc-tion to K3H2 by NADPH followed by direct or enzymatic reoxidation of the quinol by oxygen, this possibility has been excluded by several observations. It is concluded that the catalytic mechanism does not involve the formation of K3H2. Solubiliza-tion of microsomes with steapsin and subse-quent fractionation with ammonium sulfate yields a soluble preparation which shows K3-dependent NADPH oxidase activity but is practically incapable of oxidizing NADPH in the absence of K3. It seems that liver microsomes contain a K3-requiring NADPH oxidase in addition to the system responsible for the slow oxidation of NADPH in the absence of added cofactor.抄録全体を表示PDF形式でダウンロード (883K) -
I. 7S Antibodies against Ovalbumin Produced by Various Lymphoid TissuesJIRO KOYAMA, NOBUKO MIYAJIMA1966 年 60 巻 2 号 p. 184-190
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーTo study the heterogeneity of guinea pig 7S γ-antibody, the incorporation of C14-amino acids into these proteins was studied using various lymphoid tissues from guinea pigs which were hyperimmunized with ovalbumin.
1. The spleen, bone marrow, mesenteric lymph nodes, granuloma at the site of injec-tion and lung actively incorporated C14-amino acids into both 7S γ1- and 7S γ2-antiovalbumin antibodies. However, different electrophoretic patterns of the C14-labelled 7 S antibodies were obtained from materials from different sources.
2. Furthermore, in all tissues examined, it was found that 7S γ1-globulin fractions had higher antiovalbumin antibody contents than 7S γ2-globulin fractions.
3. From these results, it was suggested that guinea pig 7S γ1- and 7S γ2-antibodies are synthesized by different types of plasma cells and also that electrophoretically different 7S γ2-antibodies are synthesized by different cells.
The present authors wish to express their sincere thanks to Prof. I. Yamashina for his encouragement during this work.抄録全体を表示PDF形式でダウンロード (507K) -
I. Lipids in Liver Cell Fractions of Rats Fed a High Fat and Cholesterol DietYUICHI TAKASUGI, YOH IMAI1966 年 60 巻 2 号 p. 191-196
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーRats were fed diets containing cholesterol and coconut oil or linoleic acid. The con-centration of lipids was determined in serum as well as in ultracentrifugal fractions of the liver.
Feeding with high fat alone produced some minor fatty infiltration in the liver, but in cholesterol-fed rats the administration of high fat accentuated the lipid deposition. The major location of the exogenous lipid deposited in the liver was associated first with a centripetally migrating fraction.
The elevation of serum cholesterol level was observed only in the cholesterol plus linoleate-fed rat.
There was a slight increase in cell particulate phospholipid level in the liver of the linoleate-fed.
The authors wish to express their deep gratitude to Professor Emeritus M. Yasuda under whose direction an early part of the work was carried out.抄録全体を表示PDF形式でダウンロード (393K) -
AKIRA OGAMO, YASUO SUZUKI, SEIICHI OKUI1966 年 60 巻 2 号 p. 197-203
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーStudies were made on a method for measuring succinate quinone reducing activity with NT as the final electron acceptor.
The rate of nonenzymatic reduction of NT by ubiquinol was compared with that of enzymatic reduction of ubiquinone, and it was found that the rate of reduction of NT by ubiquinol did not limit the rate of over all reaction.
Intact mitochondria could reduce NT without addition of quinone and this activity was inhibited by antimycin A. The reduction of NT was stimulated by addition of quinones with decrease in sensitivity to antimycin A. In this case NT was reduced both via an antimycin sensitive site and via the added ubiquinone. Therefore, the data obtained with intact mitochondria did not represent the true value of quinone reducing activity.
With acetone treated mitochondria, which cannot reduce NT without added quinone and which have lost antimycin sensitivity, data on quinone reducing activity measured with NT showed that the amount of formazan formed was exactly proportional to both the enzyme concentration and the reaction time. The initial rate of this reaction was compatible with the value obtained by the direct extrac-tion method. Therefore, the use of NT seems to be the best method for the determination of the quinone reducing activity of the mito-chondria) preparation which shows the same properties as acetone treated mitochondria.抄録全体を表示PDF形式でダウンロード (526K) -
I. Detection, Purification, and Properties of Cytochrome c-552 in Anaerobically Grown CellsTAKESHI FUJITA1966 年 60 巻 2 号 p. 204-215
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリー1. A cytochrome of the c-type, called cytochrome c-552, was found to be present in the soluble fraction obtained from the cells of E. coli, Yamagutchi strain, grown anaero-bically in a semi-synthetic medium containing NaNO3.
2. The ability to synthesize cytochrome c-552 during anaerobic growth seems to be widely distributed in E. coli and related facultative anaerobes.
3. Cytochrome c-552 was isolated and purified extensively. Its properties such as spectrophotometric characteristics, reactivities, stability, E'0, and nature of heme prosthetic group, molecular weight, and heme content were studied.
4. Purified cytochrome c-552 was found to possess unusual properties for a c-type cytochrome in that it has a strongly negative E'0 and autoxidizability; it does not combine with both CO and KCN. A molecular weight of 136, 000 was estimated by the Archibald method. On the other hand, minimum molecular weights calculated from the heme and iron contents were 12, 300 and 11, 100, respectively.
The author wishes to express his gratitude to Professor R. Sato for his helpful advice and encouragement during this work, and would like to thank Dr. E. Itagaki, for his useful discussion to this work.抄録全体を表示PDF形式でダウンロード (2101K) -
KAZUO FUJIKAWA, TOMOJI SUZUKI, KIYOSHI KURAHASHI1966 年 60 巻 2 号 p. 216-218
発行日: 1966/08/25
公開日: 2008/11/18
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TOSHIHARU NAGATSU, KUNIO YAGI1966 年 60 巻 2 号 p. 219-221
発行日: 1966/08/25
公開日: 2008/11/18
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RYOHEI OGURA, MAKOTO SEIJI1966 年 60 巻 2 号 p. 222-224
発行日: 1966/08/25
公開日: 2008/11/18
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HIROSHI MORIYA, KEIKO YAMAZAKI, HIROKO FUKUSHIMA1966 年 60 巻 2 号 p. 225-227
発行日: 1966/08/25
公開日: 2008/11/18
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TADASHI SHIMOJO, KIMIYOSHI OHNO1966 年 60 巻 2 号 p. 228-230
発行日: 1966/08/25
公開日: 2008/11/18
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KOUI TAKAHASHI, TSUTOMU YASUI1966 年 60 巻 2 号 p. 231-232
発行日: 1966/08/25
公開日: 2008/11/18
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SHIGERU TAKANASHI, TSUTOMU KAWAGUCHI, YOSHIKAZU HINOHARA, MINORU KAWAD ...1966 年 60 巻 2 号 p. 233-235
発行日: 1966/08/25
公開日: 2008/11/18
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KUNIO YAGI, AKIRA KOTAKI, MAKOTO NAOI, KENTARO OKAMURA1966 年 60 巻 2 号 p. 236-238
発行日: 1966/08/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (211K)
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