巻号一覧
61 巻, 2 号
選択された号の論文の19件中1～19を表示しています
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I. General Features of the Processes of Phosphorolysis and ArsenolysisKENJI ISHII, SATORU SHIMIZU, ISAMU SHIIO1967 年 61 巻 2 号 p. 153-161
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーA modified method for preparing 170-fold purified polynucleotide phosphorylase [EC 2. 7. 7. 8] from Azotobacter agilis and general features of the processes of phosphorolysis and arsenolysis of highly polymerized yeast RNA were described. The products of the above reactions were four nucleoside 5'-diphosphates and nucleoside 5'-monophosphates, respectively.
Similar results concerning the effect of substrates and KCl concentration and a little different results in respect to the effect of MgCl2, optimal pH, degradation velosity at optimal condition were obtained with the two reactions. MnCl2 can replace MgCl2 as a cofacter. A higher activity (2.7 times) of MnCl2 than MgCl2 was observed at a low concentration (0.5mM).抄録全体を表示PDF形式でダウンロード (647K) -
MASAHIKO KODAMA1967 年 61 巻 2 号 p. 162-167
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリー1. The interaction of mitomycin C (MC) with DNA has been investigated in vitro. Using the flow-dichroism method, MC was found to be oriented parallel to the DNA bases. The viscosity study revealed that MC increased the viscosity of DNA solution. These two results support the assumption of intercalation of MC into the base-pairs of DNA helix.
2. Using the difference spectrum method, purine bases were found to be more likely binding sites than pyrimidine bases.
3. The biological action of MC is discussed in connection with the in vitro interaction.抄録全体を表示PDF形式でダウンロード (457K) -
The Mode of Action of an Alkaline Proteinase from Streptomyces griseus on Baker's Yeast Cytochrome cAKIRA HIRAMATSU1967 年 61 巻 2 号 p. 168-178
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーIn order to elucidate the specificity of the alkaline proteinase from Streptomyces griseus (ATCC 3463) on protein substrates of known primary structures, baker's yeast (Saccharomyces oaiformis) cytochrome c was used. The peptides in the hydrolyzate of the NES-cytochrome c were fractionated by chromatography on a column of Dowex 50×2 into thirtyeight peaks. Most of the peaks were not homogeneous and they were separated totally into sixty-six peptides and six free amino acids.
The amino acid and the N-terminal analyses of these peptides were carried out to find the exact positions to locate these peptides in the linear structure of the cytochrome. All the isolated peptides could be located in the known primary structure of the cytochrome without inconsistency. The alkaline proteinase hydrolyzed quickly the arginyl, lysyl, histidyl, phenylalanyl, tyrosyl, asparaginyl, leucyl, methionyl, and alanyl linkages, but not prolyl, aspartyl and cystinyl bonds in the cytochrome c. Therefore the enzyme possesses quite a broad specificity.抄録全体を表示PDF形式でダウンロード (664K) -
II. Pigments and Lipids in Blue-green Alga, Anacystis nidulansOSAMU HIRAYAMA1967 年 61 巻 2 号 p. 179-185
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーThe pigment, quinone, and lipid compositions were determined in Anacystis whole cells and lamellae, and a comparison was made with the findings in whole leaves and lamellae of spinach. In general, Anacystis was simpler than spinach in compositions of these substances, lacking some components such as lecithin, phosphatidyl inositol, tocopherol, tocopherolquinone, sterol, sterol ester, and polyenoic acids which are commonly discovered in higher plants.抄録全体を表示PDF形式でダウンロード (391K) -
KIYOTO GOTO, MASATAKA HIGUCHI, HIDEAKI SAKAI, GORO KIKUCHI1967 年 61 巻 2 号 p. 186-192
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーEffects of various factors on the induced syntheses of aminolevuli-nate synthetase, aminolevulinate dehydratase [EC 4. 2. 1. 24] and bacteriochlorophyll in dark-aerobically grown R. spheroides cells were studied.
The induced increase of aminolevulinate synthetase was completely inhibited by vigorous aeration and by addition of hemin to the incubation mixtures. Hemin, however, scarcely inhibited the induced synthesis of bacteriochlorophyll. The induced syntheses of aminolevulinate synthetase and bacteriochlorophyll were both strongly inhibited when aminolevulinate was added before the start of incubation. However, when aminolevulinate was added after the commencement of active synthesis of bacteriochlorophyll, the pigment synthesis was not significantly inhibited, in contrast to that the synthesis of aminolevulinate synthetase was immediately and completely inhibited on addition of aminolevulinate. The features of the induced increase of aminolevulinate dehydratase were similar in many respects to those of aminolevulinate synthetase. Omission of iron from the incubation mixtures resulted in nearly complete suppression of bacteriochlorophyll synthesis, but the induction of aminolevulinate synthetase was not suppressed at least in earlier periods of the incubation. Acetate as well as citrate significantly inhibited the bacteriochlorophyll synthesis, but did not inhibit the synthesis of aminolevulinate synthetase.
It is suggested that the induced synthesis of aminolevulinate synthetase is not necessarily related to the induced formation of bacteriochlorophyll. The possible difference between regulatory mechanisms of the induced syntheses of aminolevulinate synthetase and bacteriochlorophyll is discussed.抄録全体を表示PDF形式でダウンロード (529K) -
YOSHIKO KAKUTANI1967 年 61 巻 2 号 p. 193-198
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリー1. Carotenoids, ubiquinone, sterol and an unknown quinone were found to be present in the cells of Sporobolomyces shibatanus.
2. The ubiquinone found was identified as CoQ10 by its behavior upon paper chromatography.
3. The unknown quinone showed absorption maxima at 265 and 272mμ in hexane and 274mμ in ethanol. It also exhibited a spectral change resembling that of ubiquinone on reduction with sodium borohydride.
4. The biosyntheses of isoprenoids in the cells of yeast were shown to vary during the course of cell growth.
5 DPA was observed to inhibit not only the production of carotenoids but also the syntheses of other isoprenoids.抄録全体を表示PDF形式でダウンロード (405K) -
XII. Effect of pH on the Stability of LysozymeKYOKO OGASAHARA, KOZO HAMAGUGHI1967 年 61 巻 2 号 p. 199-210
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーIn order o know the role of ionizable groups in the stability of the lysozyme [EC 3.2.1.17] molecule, the effect of pH on the optical rotatory and ultraviolet spectral properties was investigated. The optical rotatory properties of lysozyme did not change at pH's between 8 and 1. However, the acid-vs-neutral difference spectra of lysozyme have peaks characteristic of the tryptophan residues. However, in the presence of 5M GuCl, in which lysozyme molecule is completely denatured, such an acid-vs-neutral difference spectrum was not observed. This suggests that a strong interaction exists between tryptophan residues and ionizable groups in the native lysozyme molecule. In the presence of 3.58M GuCl, the values of De at 292 m, o and the Moffitt parameters, a0, and b0, changed greatly at pH's below 4.0, with an apparent pK value of 3.2. The equilibrium constant between the native and denatured lysozyme in the presence of 3.84M GuCl is proportional to the 1.7th power of the hydrogen ion concentration ([H+]). The acid denaturation of lysozyme in the presence of GuCl obeyed a reversible first order reaction kinetics. The apparent rate constant, k, in the presence of 3, 84M GuCl was expressed by k=k1'[H+]1.2+k2'/[H+]0.8. The results obtained here suggest that carboxyl groups which have a pK value of 3.2 and interact strongly with tryptophan residues play an important role in the stabilization of the lysozyme molecule.抄録全体を表示PDF形式でダウンロード (744K) -
VI. Control of Glycolysis by ATP Level in Human ErythrocytesTAKAYASU SAITO, SHIGEKI MINAKAMI1967 年 61 巻 2 号 p. 211-219
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリー1. Human erythrocytes with varying ATP levels were prepared and the process of lactate formation from glucose was studied. Lactate formation increased as the intracellular ATP concentration increased. Remarkable increases of triose phosphates and fructose diphosphate were observed.
2. If lactate formation was determined using inosine as a substrate in the cells containing varying concentrations of ATP, the lactate forma-tion decreased with a concomitant increase in triose phosphate level as the ATP concentration increased. This reduction of lactate formation was relieved by the addition of deoxyglucose.
3. Increase of 2, 3-diphosphoglycerate was observed either on increasing ATP concentration or on the addition of methylene blue. The addition of arsenate caused both an increase in lactate formation and a decrease of 2, 3-diphosphoglycerate level.
4. Some increase of the glycolytic rate was observed on increasing the intracellular NAD concentration.
5. The possibility was discussed that phosphoglycerate kinase [EC 2. 7. 2. 3] and aldolase [EC 4. 1. 2. 13] may serve as additional rate-limiting factors in the presteady state glycolysis in erythrocytes.抄録全体を表示PDF形式でダウンロード (610K) -
ATSUMI KAMOGAWA, KIYOSHI KURAHASHI1967 年 61 巻 2 号 p. 220-230
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーA mutant strain of Escherichia coli K 12 which could not grow on glucose as a sole carbon source was found to have a defect in the transport of glucose. However, this inability of the cells to utilize glucose was found to be restored when the cells were grown on galactose, lactose or n (+)-fucose, a non-metabolizable inducer of the enzymes of galactose metabolism. It was concluded that glucose was transported through the galactose transport system in this mutant cell.
When glucose was added to the culture of the cells growing in a mineral galactose medium, the growth of the cells was inhibited. This inhibitory effect of glucose on the growth of the mutant cells growing on galactose was due to the repression of the formation of galactose transport system by glucose which was taken up by the cells through the galactose transport system. It is suggested that diauxie between glucose and galactose may be due to the repression of the formation of galactose transport system and not due to the competitive effects of glucose on the transport of galactose.抄録全体を表示PDF形式でダウンロード (815K) -
II. Crystallization and Properties of Alkaline PhosphataseKAZUMASA TAKEDA, AKIRA TSUGITA1967 年 61 巻 2 号 p. 231-241
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーA procedure for the purification and crystallization of alkaline phos-phatase [EC 3. 1. 3. 1] of B. subtilis is described, and several chemical properties of the purified enzyme are reported. The enzyme appears when the cells are grown under the condition of inorganic phosphate deprevation and is probably located in the cell tightly bound to the cell membrane or wall. The enzyme activity is inhibited with EDTA and this inhibition is recovered by the addition of cobalt or zinc ion. The amino acid composition and amino terminal residue of alkaline phosphatase have been determined.抄録全体を表示PDF形式でダウンロード (2505K) -
HIROSHI NAKAMURA, YUJI TONOMURA1967 年 61 巻 2 号 p. 242-250
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリーG-actin was polymerized by adding 30mM KCl at pH 7.5 and 16-20°C for 20-30 hours at various concentrations of protein (0.04-1.2mg. per ml.) in the presence of 0.1mM ATP and 1mM dehydroacetate. The binding of polymerized actin with myosin or H-meromyosin, the extent and rate of superprecipitation and the activity of ATPase [EC 3. 6. 1. 3] of actomyosin reconstituted from myosin and actin were measured, and the following results were obtained : When G-actin was polymerized at extremely low protein concentrations, myosin and H-meromyosin scarcely bound with the type of polymerized actin obtained (F'-actin-ADP). The increase intensity of light-scattering due to the binding of myosin or H-meromyosin to actin occurred in parallel with the actin concentration used for polymerization and reached a constant value when the concentration used for polymerization was higher than 0.6mg. per ml. The light-scattering intensity from 30° to 135° of the system composed of F'-actin-ADP (0.04mg. per ml.) and myosin (0.12mg. per ml.) was equal to the sum of those myosin and F'-actin-ADP, and was unchanged on the addition of 1mM MgPP1.
The time dependence and the final extent of superprecipitation (induced by ATP) of the mixture of myosin and polymerized actin were scarcely affected by the actin concentration for polymerization. On the other hand, the enhancement of ATPase activity of myosin by actin depended on the actin concentration for polymerization; the ATPase activity of the F'-actin-ADP-myosin system was only less than 20 per cent of that of the usual actomyosin. These results suggest that the actomyosin type of enzymatic activity is unnecessary for the occurrence of superprecipitation of actomyosin.
Under the condition in which the superprecipitation of actomyosin took place, no formation of C14-ITP or C14-ATP was detected in the F-actin-C14-IDP-myosin-ATP system or the F-actin-C14-ADP-myosin-ATP system. This result suggests that the nucleotide bound to F-actin exchanges with nucleotides in the medium during superprecipitation of actomyosin as a result of loosening of actin-nucleotide linkage, and that the ADP or IDP bound to F-actin is not the acceptor of the phosphate group from the reactive myosin-phosphate complex.抄録全体を表示PDF形式でダウンロード (653K) -
TETSUO UETE1967 年 61 巻 2 号 p. 251-257
発行日: 1967/02/25
公開日: 2008/11/18
ジャーナル フリー1. Amino acid incorporation into proteins of rat liver and thymus nuclei were compared using cell free systems.
2. Both thymus and liver nuclei incorporated amino acids into their proteins without addition of high-energy phosphates. Amino acid incorporation into thymus nuclear proteins was not modified by ATP, CTP, GTP, and UTP added to the incubation medium, whereas amino acid incorporation into liver nuclear proteins was stimulated by ATP and GTP. In the presence of phosphoenolpyruvate and pyruvate kinase [EC 2. 7. 1. 40] in the incubation medium, oligomycin, an inhibitor of oxidative phosphorylation, inhibited amino acid incorporation into thymus nuclear proteins and this inhibition was not restored by ATP added. Under the same conditions oligomycin did, however, not inhibit amino acid incorporation into liver nuclear proteins.
3. Potassium and sodium ions inhibited amino acid incorporation into rat thymus nuclear proteins under the conditions of this study, but these ions stimulated amino acid incorporation into liver nuclear proteins.
4. RNase [EC 2. 7. 7. 16] inhibited amino acid incorporation into liver nuclear proteins, but it did not affect amino acid incorporation into thymus nuclear proteins. DNase [EC 3. 1. 4. 5] did not affect amino acid incorporation into both liver and thymus nuclear proteins.抄録全体を表示PDF形式でダウンロード (482K) -
SHIZUKO TAKAHASHI1967 年 61 巻 2 号 p. 258-259
発行日: 1967/02/25
公開日: 2008/11/18
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TAKASHI HASHIMOTO, HARUHISA YOSHIKAWA1967 年 61 巻 2 号 p. 260-262
発行日: 1967/02/25
公開日: 2008/11/18
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SHIN'ICHI OKUDA, HAJIME TAKAHASHI1967 年 61 巻 2 号 p. 263-264
発行日: 1967/02/25
公開日: 2008/11/18
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GENJI MATSUDA, TETSUO MAITA, NAGATOSHI IGAWA, HISAHIRO OTA, MASATO YAM ...1967 年 61 巻 2 号 p. 265-268
発行日: 1967/02/25
公開日: 2008/11/18
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DAVID R. KOMINZ, KOSCAK MARUYAMA1967 年 61 巻 2 号 p. 269-271
発行日: 1967/02/25
公開日: 2008/11/18
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CHEN-CHUNG YANG, CHUN-CHANG CHANG, KOZO HAMAGUCHI, KIYOSHI IKEDA, KYOZ ...1967 年 61 巻 2 号 p. 272-274
発行日: 1967/02/25
公開日: 2008/11/18
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KEI TSUZURAHARA, MASAYASU KITAGAWA, YUICHI YAMAMURA1967 年 61 巻 2 号 p. 275-276
発行日: 1967/02/25
公開日: 2008/11/18
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