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61 巻, 3 号
選択された号の論文の16件中1～16を表示しています
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SHINSUKE OHNO1967 年 61 巻 3 号 p. 277-282
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーStudies on the antiviral actions of interferon (If) were carried out. The results obtained may be summarized as follows.
The inhibition by If of the induction of thymidine kinase [EC 2. 7. 1. 21] activity in vaccinia virus-infected chick embryo cells and a significant inhibition of the thymidine kinase induction in the infected cells treated with mouse RNA were observed. Enzyme activities of If-untreated and CAM extract-treated infected control cells increased markedly following infection, while that of If-pretreated infected cells did not until 6 hours. At 6 hours p.i., the level of enzyme activity of If-pretreated infected cells was about 10% of untreated infected cells. When the infected cells were treated with mouse RNA, a noticeable inhibition of enzyme induction was observed, whereas the inhibition by chick embryo RNA was relatively weak. A considerable degree of reduction of virus formation in cultures treated with If or mouse RNA was attained as well.抄録全体を表示PDF形式でダウンロード (444K) -
II. 7S Antibodies against p-Azobenzenearsonate-bovine γ-Globulin Conjugate Produced by Various Lymphoid TissuesJIRO KOYAMA, NOBUKO MIYAJIMA1967 年 61 巻 3 号 p. 283-289
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーTo further study the heterogeneity of guinea pig 7S γ-antibodies, the incorporation of C14-amino acids was investigated using various lymphoid tissues from guinea pigs which had been hyperimmunized with AS-BGG* conjugate.
1. The spleen, bone marrow and mesenteric lymph nodes incorpo-rated C14-amino acids into the 7S γ1- and 7S γ2-antibodies. However, different electrophoretic patterns of the C14-labelled 7S antibodies were obtained from different lymphoid tissues.
2. Furthermore, it was revealed that the 7S anti-AS antibody patterns were different from those of 7S anti-BGG antibodies produced by the same tissue.
3. From these results, it was concluded that guinea pig 7S antibody patterns are deeply affected by the immunochemical specificity of determinants of immunizing antigen.抄録全体を表示PDF形式でダウンロード (505K) -
IX. Biochemical Studies (4). Separation and Substrate Specificity of Pantothenate Kinase and Phophopantothenoylcysteine SynthetaseYASUSHI ABIKO1967 年 61 巻 3 号 p. 290-299
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリー1. To investigate the pathway of biosynthesis of CoA in rat liver, pantothenate kinase [EC 2. 7. 1. 33] and phosphopantothenoylcysteine synthetase [EC 6. 3. 2. 5] have been completely separated from. each other.
2. Based on the specificity of these two separate enzyes, it was suggested that pantothenate was first phosphorylated and then condensed with cysteine to form phosphopantothenoylcysteine in the course of CoA biosynthesis in rat liver.
3. It has been shown that rat-liver pantothenate kinase catalyzes the phosphorylation not only of pantothenate and panteth(e)ine but also of pantothenoylcyst(e)ine.抄録全体を表示PDF形式でダウンロード (777K) -
X. Biochemical Studies (5). Purification and Substrate Specificity of Phosphopantothenoylcysteine Decarboxylase from Rat LiverYASUSHI ABIKO1967 年 61 巻 3 号 p. 300-308
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリー1. Phosphopantothenoylcysteine decarboxylase [EC 4. 1. 1. 36] was highly purified from rat liver and some properties of the decarboxylase are reported.
2. Using a crude preparation and the purified enzyme, it was confirmed that the enzymatic decarboxylation of the cysteine moiety in the course of CoA biosynthesis in rat liver occurred with phosphopantothenoylcysteine but not with pantothenoylcysteine as the substrate.抄録全体を表示PDF形式でダウンロード (612K) -
XI. Biochemical Studies (6). A Final Stage in the Biosynthesis of CoAYASUSHI ABIKO, YADAO SUZUKI, MASAO SHIMIZU1967 年 61 巻 3 号 p. 309-312
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリー1. The final steps of CoA biosynthesis in rat liver were investigated using purified dephospho-CoA pyrophosphorylase [EC 2. 7. 7. 3] and dephospho-CoA kinase [EC 2. 7. 1. 24] and phospho pantothenoylcysteine decarboxylase [EC 4. 1. 1. 36].
2. It was concluded that the pathway involving the following metabolic conversions is the only operative one: phosphopantetheine→dephospho-CoA→CoA.
3. The possibilities of other reaction sequences were also examined and excluded.抄録全体を表示PDF形式でダウンロード (312K) -
HIROMICHI OKUDA, SETSURO FUJII1967 年 61 巻 3 号 p. 313-319
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーRat epididymal adipose tissue contained lipase [EC 3. 1. 1. 3] and esterase [EC 3. 1. 1. 1] which were separated by ammonium sulfate fractionation and gel filtration on a Sephadex G-200 column. On the other hand, rat liver contained a lipolytic enzyme, which seems to correspond to lipase, as judged from its behaviour on ammonium sulfate fractionation and gel filtration on Sephadex G-200, and from its sub-strate specificity.
When the adipose tissue and liver lipase were treated with acetone, their behaviours on gel filtrations and substrate specificities were con-verted into those of the adipose tissue esterase.抄録全体を表示PDF形式でダウンロード (525K) -
MICHIHIRO SUGANO, ISAO CHINEN, MASAFUTO WADA1967 年 61 巻 3 号 p. 320-327
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーThe rate and fatty acid specificity of cholesterol esterification in plasma were studied in the male and laying chickens after intravenous administration of cholesterol-4-C14 in serum lipoproteins. The laying resulted in a decreased cholesterol esterification. The quantity of cholesterol esterified in vivo (expressed as μg./ml. plasma/hour) was 52-88 for the male and 13-38 for the laying female. The initial rate of plasma cholesterol esterification was considerably rapid in the male, and the difference between the male and laying female was consistent with that observed in the in vitro studies (14-16). In addition, equilibration of cholesterol esters derived from exogenous cholesterol with the endogenous esters apparently occurred in 2 days in the male and between 2-3 days (or later) in the layer.
Esterification of plasma cholesterol with different fatty acids appeared to proceed heterogeneously shortly after administration of the labeled serum. In general, the arachidonate ester had initially a higher relative specific activity and the saturated or monounsaturated (only in the case of the male) esters had a low activity. These initial heterogeneous patterns of relative specific activities of the different fatty acid esters disappeared as the time elapsed, and became homogeneous in 2 days in the male, while still not in 4 days in the laying female. The initial heterogeneity observed in vivo was similar to that seen in the in vitro experiments.
These results as well as those reported previously suggested that the plasma-esterifying activity has a significant role in the composition and the level of plasma ester cholesterol.抄録全体を表示PDF形式でダウンロード (561K) -
XII. Amino Groups with Different Reactivities toward Naphthoquinone Sulfonic AcidAYAKO MATSUSHIMA, YUTAKA HACHIMORI, YUJI INADA, KAZUO SHIBATA1967 年 61 巻 3 号 p. 328-336
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーβ-Naphthoquinone-4-sulfonic acid (NQS) was examined and applied to several proteins as a reagent for discriminating various states of amino groups in the tertiary structures of proteins, and the following facts were revealed. NQS possesses a moderate reactivity suitable for the discrimination at room temperature and between pH 7.5 and 9.3 where most proteins are native, and the degree of reaction can be determined simply by absorption photometry at 480mμ. Four of the total 7 amino groups in the lysozyme [EC 3. 2. 1. 17] molecule, 7 of the 11 amino groups in ribonuclease [EC 2. 7. 7. 16], 14 of the 17 groups in α-chymotrypsin [EC 3. 4. 4. 5], 12 of the 15 groups in chymotrypsinogen and 12 of the 17 groups in diisopropylphosphoryl-chymotrypsin are more reactive with NQS than the remaining 3, 4, 3, 3 and 5 groups, respectively. Two terminal amino groups of the A and B chains of insulin are reactive, and the remaining ε-amino group of B29 Lys is not reactive with this reagent. The non-reactive ε-amino group is transformed into the reactive type by alkali denaturation but is not transformed by tryptic digestion. It was deduced from these results that the ε-amino group is hydrogen-bonded with a residue in the heptapeptide released by the digestion. The counterpart in the hydrogen-bonding was inferred to be the hydroxyl group of B26 Tyr or B27 Thr.抄録全体を表示PDF形式でダウンロード (708K) -
XIII. Monochlorotrifluoroquinone as a New Reagent for Discrimination of Amino GroupsKAZUYASU NAKAYA, HIROO HORINISHI, KAZUO SHIBATA1967 年 61 巻 3 号 p. 337-344
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーA new reagent, monochlorotrifluoro-p-benzoquinone (abbreviated to CFQ) was explored as a means for discriminating amino groups in proteins, and the following facts were revealed, applying the reagent to proteins. The reagent possesses a moderate reactivity suitable for the discrimination; 1, 1, 3, 7, 8, 10 and 8 out of the total 2; 3, 7, 11, 15, 17 and 17 amino groups in the molecules of glucagon, insulin, lysozyme [EC 3. 2. 1. 17], pancreatic ribonuclease [EC 2. 7. 7. 16], chymotrypsinogen, α-chymotrypsin [EC 3. 4. 4. 5] and diisopropylphosphoryl(DIP)-chymo-trypsin, respectively, are reactive with this reagent. The reactivity of CFQ is, therefore, lower than that of β-naphthoquinone-4-sulfonic acid (NQS). The terminal α-amino group of B1 Phe in the insulin molecule is the single amino group reactive with CFQ, and the α-amino group of A1 Gly and the α-amino group of B29 Lys are the non-reactive type. In terms of the reactivities with CFQ and with NQS, the amino groups of insulin can be classified into three types; the α-amino group of B1 Phe reactive both with CFQ and with NQS, the α-amino group of A1 Gly reactive with NQS but not reactive with CFQ and the ε-amino group of B29 Lys not reactive with these reagents. A possibility of intra-chain hydrogen bonding of the amino group of A1 Gly with the carboxyl group of A4 Glu was discussed.抄録全体を表示PDF形式でダウンロード (609K) -
XIV. Glyoxal as a Reagent for Discrimination of Arginine ResiduesYAZUYASU NAKAYA, HIROO HORINISHI, KAZUO SHIBATA1967 年 61 巻 3 号 p. 345-351
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーSeveral aldehydes and ketones were examined in order to explore a reagent suitable for discrimination of various states of arginine residues in proteins in terms of reactivity. The chemicals examined were glyoxal, formaldehyde, pyruvaldehyde, diacetyl and dibenzoyl, and the examinations were made with native and denatured insulin and its B chain and lysozyme [EC 3. 2. 1. 17] as the sample proteins with arginine residues. Glyoxal was most reactive of these chemicals, and the single B22 arginine residue of the B chain and the 11 arginine residues of lysozyme reacted to the extent of approximately 80% with this reagent, while other essential amino acids were not appreciably affected by the treatment. Practically no reaction of lysine residues, which is generally more reactive than arginine residues, is interpreted as due to an artifact; lysine residues modified with glyoxal seem to be hydrolyzed back to intact lysine on the acid treatment of glyoxalmodified protein for the assay of arginine. The B22 arginine residue in native insulin was much less reactive with glyoxal than the same residue in alkali-denatured insulin or in the B chain. This result supported strongly a previous view that the B26 tyrosine residue is hydrogen-bonded with the B22 arginine residue in the native insulin molecule.抄録全体を表示PDF形式でダウンロード (573K) -
KAZUE OZAWA, KIICHIRO SETA, HIROSHI ARAKI, HAJIME HANDA1967 年 61 巻 3 号 p. 352-358
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーBrain mitochondria differed from mitochondria from other tissues (e.g., liver, kidney and heart mitochondria) in showing an increased rate of state 3 respiration in media containing high concentrations of potassium ions. The potassium-stimulated respiration of brain mitochondria increased remarkably with either glutamate or succinate as substrate but followed a different course with the two substrates. The respiratory rate with succinate reached a maximum at about 50mM of potassium ions, while with glutamate the maximum was reached with higher concentrations (about 100mM) of potassium ions.
Similar stimulatory effects of potassium ions were observed on respiration activated by 2, 4-dinitrophenol. In addition, potassium ions were found to compete with magnesium ions in the stimulation of state 3 respiration with NAD-linked substrates.
From these results, it was suggested that potassium ions exert a direct stimulatory effect on the electron transport system.抄録全体を表示PDF形式でダウンロード (1489K) -
KAZUKO HARA, HIROMI NITSUI1967 年 61 巻 3 号 p. 359-366
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーExperimental evidence was presented indicating that the loss of DNA dependent RNA polymerase [EC 2. 7. 7. 6] activity in E. cola which is infected with bacteriophage T2 is due to the degradation of enzyme molecule detached from the host DNA. An enzyme preparation extracted from a deoxyribonucleoprotein-protamine complex was used as a model undergoing degradation with a loss of enzyme activity. The degradation profile of the enzyme in the preparation was studied with special reference to the changes of its molecular size and activity. The native enzyme molecule showed a sedimentation constant of 16S. On storage at 2°C, the activity of the enzyme decreased gradually and the enzyme molecule degraded to give rise to smaller subunits with release of some component. Addition of this component to the inactivated enzyme resulted in the complete recovery of the enzyme activity.抄録全体を表示PDF形式でダウンロード (1037K) -
TAKAO MINAMIKAWA, IKUZO URITANI1967 年 61 巻 3 号 p. 367-372
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリー1. Extracts of sweet potato roots were shown to have the activity of DAHP synthase [EC 4. 1. 2. 15], which catalyzes the conversion of E4P and PEP to DAHP. The product formed by action of the enzyme was identified as DAHP by the absorption spectrum of the derivative formed by periodate oxidation and TBA treatment. The product of the reaction was also identified by paper chromatography of the reaction mixture.
2. Mg++ at 1mM concentration increased the rate of DAHP formation by 65 to 120%. K+ (50mM) had no effect. Addition of reduced glutathione (1 to 3mM) to either the enzyme extraction medium or reaction mixture was not effective.
3. The root tissues were sliced and placed at 28-29°C, and DAHP synthase activity was analyzed at 24-hour intervals for 4 days. DAHP synthase activity was found to remain relatively constant without marked change due to slicing.
4. Several plants were assayed for DAHP synthase activity. Of the plants tested, the activity was detected only in Trifolium repens. Mg++ (5mM) was required for maximum activity.抄録全体を表示PDF形式でダウンロード (461K) -
I. Inhibition of Protein SynthesisSATOSHI MIZUNO, KAZUO NITTA, HAMAO UMEZAWA1967 年 61 巻 3 号 p. 373-381
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーMode of action of enomycin, an antitumor antibiotic, was studied in cellular metabolism in vitro. Although enomycin weakly inhibited protein synthesis in intact cells, it exhibited strong inhibition of protein synthesis by endogenous messenger RNA in cell-free system of Ehrlich ascites carcinoma cells, HeLa cells and rat liver cells at a concentration as low as 10-8 M. Enomycin inhibited C14-lysine and C14-proline incorporation which was weakly stimulated by poly A and poly C, respectively. The inhibitory effect on C14-phenylalanine incorporation stimulated by poly U was antagonized by high concentration of poly U.
Enomycin showed almost no inhibitory effect on protein synthesis in cell-free system of E. coli.
Enomycin did not affect the transfer of amino acids to sRNA but inhibited the transfer of amino acids from aminoacyl-sRNA into protein on ribosomes of Ehrlich ascites carcinoma cells.抄録全体を表示PDF形式でダウンロード (727K) -
II. Preferen ial Binding of Enomycin to Mammalian RibosomesSATOSHI MIZUNO, KAZUO NITTA, HAMAO UMEZAWA1967 年 61 巻 3 号 p. 382-387
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーEnomycin binds to ribosome-monomers, -dimers and -trimers active in protein synthesis of Ehrlich ascites carcinoma cells. Enomycin binds to ribosomal sub-units of the carcinoma cells. Enomycin inhibits the binding of aminoacyl-sRNA to polyribosomes and suppresses protein synthesis.
Enomycin does not bind to ribosomes of E. coli.抄録全体を表示PDF形式でダウンロード (383K) -
XVI. Significance of the Molecular State fo Cytochrome a for Oxidase ActivityYUTAKA ORII, KAZUO OKUNUKI1967 年 61 巻 3 号 p. 388-403
発行日: 1967/03/25
公開日: 2008/11/18
ジャーナル フリーThe cytochrome oxidase [EC 1. 9. 3. 1] activity of cytochrome α increased two to three fold on treatment with SDS or guanidine hydro-chloride. Sedimentation analyses revealed that SDS treatment resulted in formation of 16.6 S and 5.7S components from the original 22.6 S component. Unlike SDS treatment; guanidine hydrochloride treatment produced only the 13S component. Sucrose density gradient studies also confirmed successive depolymerizations of cytochrome a induced by SDS and molecular weights of 290, 000-330, 000 and 67, 000 were assigned to the 16.6 S and 5.7 S components, respectively, on the basis of a value of 530, 000 for the original material. Analyses of the haem a content on the basis of the dry weight gave a minimum molecular weight of 128, 000, and calculations suggested that the original polymer with a molecular weight of 530, 000 contained 4 molecules of haem a while the 16.6S species contained 2 molecules of haem a. The molecular weight assigned to the smallest unit was about half that of the hypothetical species of 128, 000 and half of the smallest species was thought to contain haem a. The activity was found with either the 16.6 S or 13S component but not with the smaller species. In conclusion, the smallest active unit contains two haem a molecules and two copper atoms. Investigations of spectral changes induced by these reagents as well as the mode of the cytochrome oxidase reaction in the presence of detergents and especially of SDS, suggested that the increase of activity was due to an increase in the number of active species.抄録全体を表示PDF形式でダウンロード (1936K)
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