巻号一覧
62 巻, 2 号
選択された号の論文の22件中1～22を表示しています
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I. Purification and Characterization of Two Soybean Trypsin InhibitorsMIHOKO YAMAMOTO, TOKUJI IKENAKA1967 年 62 巻 2 号 p. 141-149
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリー1. A simple method was developed for the preparative purification of large amounts of two soybean trypsin inhibitors, namely the Kunitzand the 1.9S-inhibitors.
2. These two trypsin inhibitors were characterized in terms of their major chemical and physicochemical properties, and these results were compared with those of soybean trypsin inhibitors isolated by other investigators.The Kunitz-inhibitor was shown to be identical to that isolated by Kunitz and the 1.9S-inhibitor appears to be a new innibitor. Molecular weight of the 1.9S-inhibitor was determined to be 16, 400 and this protein contains more than 19 per cent of cystine and lacks cysteine and glycine.
3. The inhibiting activites of the two inhibitors against several proteinases were investigated.抄録全体を表示PDF形式でダウンロード (2725K) -
SHINICHIRO WATANABE, YOSHIMI OKADA, MASAYASU KITAGAWA1967 年 62 巻 2 号 p. 150-160
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリー1. Rabbit antibodies against yeast cytochrome c could be detected by various immunological assays, such as active skin test, double diffusion test, quantitative precipitation test, P.C.A. test, passive hemagglutination and hemolysis, immune gel-filtration, and radioimmunoelectrophoresis. It was confirmed that the antibodies are directed to cytochrome c itself.
2. Antibodies against equine heart cytochrome c could be produced in rabbits.
3. Immunological cross reactions were observed between yeast cytochrome c and tuna heart cytochrome c and between equine and tuna heart cytochrome c but not between yeast and equine heart cytochrome c.
4. Mouse antibodies against dimer form yeast cytochrome c could be demonstrated by P.C.A. test and radioimmunoelectrophoresis. The antibody activities were detected in γ-1 and γ-2 immunoglobulins.
The homologous skin sensitizing activity was detected in γ-1 immunoglobulin, while heterologous skin sensitizing activity was detected in γ-2 immunoglobulin.抄録全体を表示PDF形式でダウンロード (1446K) -
IV. Purification of the Alanine, Valine and Isoleucine Transfer Ribonucleic Acids by Column ChromatographyMASAZUMI MIYAZAKI, MASAHARU KAWATA, KOTOE NAKAZAWA, SHOSUKE TAKEMURA1967 年 62 巻 2 号 p. 161-169
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーThe alanine, valine and isoleucine tRNA's were highly purified by chromatography on a DEAE-Sephadex column with the ammonium sulfate-DMFA, phosphate-DMFA-KCl and borate-KCl systems. The purity of the alanine tRNA I was 64-66%, of the valine tRNA I, 85% and of the isoleucine tRNA, 80%, on the basis of acceptor activity. The alanine and valine tRNA's were resolved into two components. The valine tRNA II was concentrated 11-fold over the original (the purity, 48%) and freed from the valine tRNA I.
The final preparations of the alanine, valine and isoleucine tRNA's were digested with pancreatic RNase I [EC 2.7.7.16] and fractionated on a DEAE-cellulose column in 7 M urea according to the chain length. The distribution patterns of oligonucleotide fragments thus obtained were quite characteristic of the specific tRNA's, which in turn supported that the final tRNA preparations were highly purified.抄録全体を表示PDF形式でダウンロード (1243K) -
II. Immunochemical PropertiesFujio SUZUKI, KEIHACHI FUKUNISHI, YASUSHI DAIKUHARA, YOSHIRO TAKEDA1967 年 62 巻 2 号 p. 170-178
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーAn anti-enzyme against ATP citrate lyase [EC4.1.3.8; ATP: citrate oxaloacetate-lyase (CoA-acetylating and ATP-dephosphorylating)] from rat liver was prepared in rabbits and the interaction of the enzyme and anti-enzyme was investigated. The reaction was complete within 10 minutes at 37°C or 1 hour at 0°C on mixing the enzyme and its antienzyme. At this point, over 90% of the original enzyme activity was lost with a concomitant formation of a visible precipitate.Increased enzyme activity after dietary induction is associated with an equivalent increase in the amount of protein which is immunologically indistinguishable from normal enzyme. On the contrary, decreased enzyme activity during fasting is accompanied by a comparable loss of immunologically reactive protein. The enzymes from various rat tissues, such as kidney, heart, brain and adipose tissue, cannot be distinguished from rat liver enzyme on the basis of their reactivity with rat liver anti-enzyme, while the enzymes from the livers of chick, dog. rabbit and guinea pig only react partially with the same anti-enzyme. The activities of ATP citrate lyase and fatty acid synthetase in rat liver are found to be on two immunologically distinct proteins.抄録全体を表示PDF形式でダウンロード (4510K) -
YOSHIKO KAKUTANI1967 年 62 巻 2 号 p. 179-183
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーThe contents of carotenes and sterol in the cells of Sporobolomyces shibatanus were decreased when the cells were grown on the media in the presence of antioxidants (nordihydroguaiaretic acid, D, L-α-tocopherol and p-hydroquinone). Some chelating reagents such as o-phenanthroline and α, α'-dipyridyl inhibited both cell growth and carotenogenesis. These inhibitions were partially restored by the addition of ferrous ion and manganese ion to the media. Diphenylcarbazide, another chelating reagent at lower concentration, affected the carotenogenesis but did not affect the cell growth.From these results, the requirement of oxygen for the desaturation of more saturated carotene was discussed.抄録全体を表示PDF形式でダウンロード (678K) -
I. Xylitol MetabolismTOSHIO ASAKURA, KAZUHIKO ADACHI, SHIGEKI MINAKAMI, HARUHISA YOSHIKAWA1967 年 62 巻 2 号 p. 184-193
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーXylitol was shown to be, an efficient reductant of methemoglobin either in red cells or hemolysate.The reaction product of the sugar alcohol was separated and inentified as xylulose by paper chromatography, color reactions and gas chromatography, and also enzymatically. The D/L ratio of xylulose was 19.8/6.8. The ratio was increased by the addition of NAD and decreased by the addition of NADP in hemolysate experiments.
The effects of xylitol metabolism on the level of several glycolytic intermediates were studied. Increase in lactate and triose phosphate was observed in the presence of xylitol. This phenomenon was explained by the change of NADH2/NAD ratio in the cells.抄録全体を表示PDF形式でダウンロード (1175K) -
KIYOMICHI HANABUSA, TAKASHI KANNO, SADAO ADACHI, HISAYO KOBAYASHI1967 年 62 巻 2 号 p. 194-201
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーA method for the purification and crystallization of phosphorylase [EC 2.4.1.1] b from pig muscle was described.
Conversion of pig muscle phosphorylase b to phosphorylase a and crystallization of the latter form were accomplished.
Pig phosphorylases b and a both appeared as a single component on ultracentrifugation. Phosphorylase b had a sedimentation coefficient of 8.5. No increase of the S value occurred upon conversion to phosphorylase a in the presence of more than 0.03M cysteine, while at a cysteine concentration lower than 0.03M, phosphorylase a gave heavier components. The relationship between cysteine concentration and polymerization of molecules was discussed. The presence of polymers was confirmed on disc-electrophoresis. These polymers seemed to have the activity.
The pH optimum of pig phosphorylases b and a was 6.4 and 6.2 respectively.
Assuming a molecular weight of 250, 000, it was inferred that pig muscle phosphorylase a contained 2 moles of pyridoxal 5'-phosphate per mole of the enzyme.抄録全体を表示PDF形式でダウンロード (7197K) -
HIROSHI MASUKAWA, NOBUO TANAKA1967 年 62 巻 2 号 p. 202-209
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーThe incorporation of 14C-amino acid into protein by DNA was markedly increased by the presence of kanamycin and neomycin in a cellfree system from E. coli. Less stimulation was observed with streptomycin. Kasugamycin did not significantly affect the amino acid incorporation by DNA.
3H-Thylnidine-labeled heat-denatured E. coli DNA was found to attach to the ribosomes by sucrose density gradient centrifugation and neomycin did not affect the attachment of DNA to the ribosomes.
The binding of sRNA labeled with 14C-phenylalanine, 14C-serine, or 14C-amino acids mixture to DNA-ribosome complex was investigated by the sucrose density gradient centrifugation and the Millipore filter method. Kanamycin, neomycin, or streptomycin was observed to stimulate the binding of aminoacyl-sRNA, whereas kasugamycin did not significantly affect the binding. Kasugamycin inhibited neomycin-stimulated binding of aminoacyl-sRNA to the ribosomes with DNA. The grade of stimulation of the binding was less than but parallel to that of the amino acid incorporation.
The stimulation by neomycin of the amino acid incorporation and the binding of aminoacyl-sRNA to the ribosomes with DNA were much reduced, when the ribosomes were obtained from neomycin-resistant E. coli. However, they were significantly enhanced by kanamycin or streptomycin.
The significance of the antibiotic-stimulated binding of aminoacylsRNA to the ribosomes for the antibiotic-stimulated, DNA-directed protein synthesis is discussed.抄録全体を表示PDF形式でダウンロード (829K) -
III. ATP-supported Reduction of NAD+ by SuccinateAKIRA ASANO, KATSUYUKI IMAI, RYO SATO1967 年 62 巻 2 号 p. 210-214
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーAn ATP-supported reduction of NAD+ by succinate was demonstrated in membrane fragments prepared from aerobically grown cells of Micrococcus denitrificans. Evidence was obtained to indicate that the reaction involved a reversal of electron and energy transfers in oxidative phosphorylation at the NADH-cytochrome b segment of the respiratory chain. Inhibitors of the electron transfer at this segment and those of the energy transfer of oxidative phosphorylation were all effective toward the NAD+ reduction process. Conditions affecting the rate of the reaction were also studied.抄録全体を表示PDF形式でダウンロード (691K) -
HIROKO NISHIBAYASHI, NOBORU NAKAI, RYO SATO1967 年 62 巻 2 号 p. 215-222
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリー1. Of the variously substituted naphthoquinones tested, those having a hydroxy, acetoxy, or methylamino group at position 2 or 3 showed no effects on the oxidation of NADPH by liver microsomes. The other quinones acted as cofactors and stimulated the microsomal oxidation.
2. Essentially the same specificity was observed with partly purified NADPH-specific flavoprotein as the oxidase, except that 2-or 3-acetoxy derivatives were effective cofactors for the purified enzyme.
3. The inability of the acetoxy compounds to act as cofactors for microsomes could be explained by the presence in microsomes of an active esterase rapidly hydrolyzing the compounds to ineffective 2-or 3-hydroxynaphthoquinones.
4. Naphthoquinones effective as cofactor reacted with cysteine resulting in considerable shifts in their spectra, wherease no such interactions were observed with ineffective compounds.抄録全体を表示PDF形式でダウンロード (1003K) -
III. Purification and Properties of L-Sorbose Oxidase from Trametes sanguineaYuzo YAMADA, KAZUAKI IIZUKA, Kô AIDA, TEIJIRO UEMURA1967 年 62 巻 2 号 p. 223-229
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリー1. An enzyme catalyzing the oxidation of L-sorbose by either oxygen or 2, 6-dichlorophenol indophenol was purified about 25-fold from extracts of a basidiomycete, Trametes sanguinea.
2. The reaction with oxygen as acceptor was formulated as; Lsorbose+O2→5-keto-D-fructose+H2O2.
3. In addition to L-sorbose, this preparation oxidized D-glucose, D-galactose, and D-xylose, but was inactive towards D-fructose and polyols.
4. The optimal pH was about 5.7 to 6.0 in potassium phosphate buffer.
5. Km values for L-sorbose were determined to be 2.2×10-2 M with atmospheric oxygen as acceptor and 1.0×10-1 M with 2, 6-dichlorophenol indophenol as acceptor at pH 6.0 and 30°C.
6 The L-sorbose oxidase activity was strongly inhibited by Ag+and Hg++, but not by typical sulfhydryl reagents such as p-chloromercuribenzoate and phenylmercuric nitrate.抄録全体を表示PDF形式でダウンロード (735K) -
V. Nitrite-dependent Gas Evolution in Cells Containing Cytochrome c-552TAKESHI FUJITA, RYO SATO1967 年 62 巻 2 号 p. 230-238
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリー1. Gas production from glucose-fermenting E. coli cells grown anaerobically with nitrite or ammonia as sole nitrogen source was compared. In nitrite-grown cells, gas formation during glucose fermentation was strikingly stimulated by the presence of nitrite, whereas nitrite inhibited completely the gas evolution by ammonia-grown cells.
2. The gas produced by nitrite-grown cells in response to nitrite addition consisted mostly of CO2 which seemed to have been derived from an unknown endogenous substrate. The total production of CO2 was proportional to the amount of nitrite added. Stoichiometry studies showed that one mole of nitrite was quantitatively reduced toammonia, concomitant with the evolution of one mole of CO2.
3. Evidence was presentedindicating that cytochrome c-552 was involved in this CO2 producing system coupled to nitrite reduction, because both the CO2 evolution and nitrite reduction were strikingly decreased when intact cells were freed from cytochrome c-552 by converting them into spheroplasts, and these activities could be restored by the addition of cytochrome c-552.
4. It was suggested that a sulfhydryl group and factors or sites sensitive to cyanide, azide, and HOQNO may be involved in the activity of the nitrite-dependent CO2 producing system.抄録全体を表示PDF形式でダウンロード (1177K) -
YOSHIO IMAI, RYO SATO1967 年 62 巻 2 号 p. 239-249
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリー1. The addition of various substrates of the drug hydroxylase system to aerobic liver microsomes caused several types of spectral changes which were characteristic of hemoproteins.
2. Several linesof evidence were obtained indicating that P-450 is involved in these spectral changes and the interaction of P450 with drug substrates is an obligatory step in the mechanism of drug hydroxylations.
3. Organic solvents, such as alcohols having short carbon chains also induced spectral changes resembling those induced by substrates of the aniline type.The concentrations of alcohols necessary to induce the spectral changes corresponded to those required for conformational changes in protein.
4. On the basis of these observations, several hypotheses are suggested to explain the interaction o microsomal P-450 with drug substrates, and the mechanism of drug hydroxylation is discussed.抄録全体を表示PDF形式でダウンロード (1638K) -
I. Inhibition by Carbonyl Reagents with Active Methylene GroupsKENSAKU MORIHARA1967 年 62 巻 2 号 p. 250-262
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリー1. The proteolitic activities of both crystalline native papain [EO 3. 4. 4. 10] and mercuripapain were remarkably inhibited with dimedone, diketohydrindene and N, N'-dimethyl burbituric acid, respectively, at final concentrations of 5×10-3 M.
2. The inhibitory effects of dimedone and diketohydrindene were always much greater than that of N, N'-dimethyl barbituric acid.
3.Papain inhibited with diketohydrindene was shown to be red.
4. Mercuripapain was more rapidly effected by dimedone and diketohydrindene than native papain.
5. Ninhydrin and N, N'-dimethyl alloxaninhibited native papain greatly, but scarcely inhibited mercuripapain.
6. The cyanide-activated papain was completely inhibited by Nethylmaleimide whereas the native papain was hardly affected by this reagent.
7. The sulfhydryl content of the native papain signiflcantly increased when the native papain was treated with some carbonyl reagents.
8. A thiohemiacetal in equilibrium with its dissociation form was proposed as a model of the active site of papain, and various reactions of papain were discussed on the basis of this model.
9. Hippuric acid phenylhydrazide affected the activityof the mercaptoethanol-activated papain.This mechanism of inhibition indicated that the aldehyde group involved in the active site plays the role of amino-acceptor in the enzymatic reaction.抄録全体を表示PDF形式でダウンロード (1538K) -
SHUNJI SAWAKI, HISAOKI HATTORI, Kozo YAMADA1967 年 62 巻 2 号 p. 263-268
発行日: 1967/08/25
公開日: 2011/01/25
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RADHA PANT, PRITI SHEILA LACY1967 年 62 巻 2 号 p. 269-271
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーPDF形式でダウンロード (292K) -
JUN'ICHI KAWANAMI1967 年 62 巻 2 号 p. 272-273
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーPDF形式でダウンロード (271K) -
NAGASUMI YAGO, MARIA STYLIANOU NIGHTINGALE, RALPH I. DORFMAN, ENRICO F ...1967 年 62 巻 2 号 p. 274-275
発行日: 1967/08/25
公開日: 2011/01/25
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HIROSHI MAEDA, NAKAO ISHIDA1967 年 62 巻 2 号 p. 276-278
発行日: 1967/08/25
公開日: 2011/01/25
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TAKAKO MASUI, YOKO HIJIKATA, TARO KAZUNO1967 年 62 巻 2 号 p. 279-281
発行日: 1967/08/25
公開日: 2011/01/25
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MASACHIKA IRIE, Fumio SAWADA1967 年 62 巻 2 号 p. 282-284
発行日: 1967/08/25
公開日: 2011/01/25
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EIJIRO OZAWA, SETSURO EBASHI1967 年 62 巻 2 号 p. 285-286
発行日: 1967/08/25
公開日: 2011/01/25
ジャーナル フリーPDF形式でダウンロード (257K)
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