巻号一覧
63 巻, 6 号
選択された号の論文の21件中1～21を表示しています
- |<
- <
- 1
- >
- >|
-
III. On Weak Inhibition of Polyphenylalanine Synthesis Directed by Polyuridylic AcidSATOSHI MIZUNO, KAZUO NITTA, HAMAO UMEZAWA1968 年 63 巻 6 号 p. 689-694
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーThe effect of enomycin, a polypeptide antibiotic and an inhibitor of protein synthesis of mammalian cells, on polyphenylalanine synthesis directed by added polyuridylic acid was studied with ribosomal system of Ehrlich ascites carcinoma cells. Phenylalanine polymerizing reaction stimulated by poly U was weakly but significantly inhibited by enomycin at the concentration which displayed a marked inhibition on the in-corporation of phenylalanine by endogenous messenger RNA.
The binding of phenylalanyl-sRNA to ribosomes was enchanced by added poly U, and was weakly suppressed by enomycin. The forma-tion of poly U-ribosomes complex was also blocked by enomycin slightly to the same degree.
The results suggested that enomycin might inhibit the formation of the poly U-ribosomes complex and also the binding of phenylalanyl-sRNA to the complex.抄録全体を表示PDF形式でダウンロード (329K) -
SABURO OMATA, SHOGO ICHII, NAGASUMI YAGO1968 年 63 巻 6 号 p. 695-700
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリー1. Mg++-dependent and Mn++/(NH4)2SO4-dependent RNA poly-merase [nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2. 7. 7. 6] activities in liver-nuclei increased by about 40% over the respective controls 24 hr after whole-body X-irradiation (650 r) of intact, ad libitum fed rats, and then the activities turned to subcontrol levels during the 10 days postirradiation.
2. Similar changes in the activities of liver RNA polymerase were observed on irradiation for fasted rats but not for adrenalectomized animals.
3. The induction of RNA polymerase as well as of tyrosine trans-aminase [L-tyrosine: 2-oxoglutarate aminotransferase, EC 2. 6. 1. 5] by cortisol-administration was not influenced by X-irradiation.
4. The effects of X-irradiation on Mg++-dependent and Mn++/ (NH4)2SO4-dependent RNA polymerase activities in testis, spleen and thymus of rats were also described.抄録全体を表示PDF形式でダウンロード (406K) -
XIII. The Fractionation of Clupeine YKOICHI SUZUKI, TOSHIO ANDO1968 年 63 巻 6 号 p. 701-708
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリー1. More reproducible and satisfactory chromatographic systems on CM-cellulose (see Fig. 2) were found for fractionation of whole clupeine into Y and Z fractions in place of the previous method by adsorption column chromatography on alumina.
2. Further fractionation of clupeine Y was tried on a CM-cellulose column using several buffer systems over a wide pH range. Although YI rich and YII rich fractions were obtained, complete separa-tion of these 2 components could not be achieved in the case of intact clupeine Y.
3. From the chromatogram of clupeine Y fraction, however, clupeine Y was deduced to consist of nearly equal amounts of 2 main com-ponents, YI and YII, i.e. whole clupeine is composed of only 3 main species of molecules, YI, YII and Z. The amino acid compositions other than arginine of clupeine YI and YII were estimated as follows: 2Ala, 3Ser, 2Thr, 2Pro, lGly and llle for clupeine YI and 2Ala, 2Ser, lThr, 3Pro and 2Val for clupeine Yll.
4. In the case of whole clupeine modified with trinitrobenzenesul-fonic acid, all the 3 components of clupeine were successfully fractionated as TNP-YI, TNP-Z and free YII by one step elution from a CM-cel-lulose column.抄録全体を表示PDF形式でダウンロード (514K) -
KATSURO KOIKE, TADAHIKO OTAKA, SEIICHI OKUI1968 年 63 巻 6 号 p. 709-715
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリー1. In adrenalectomized mice, 30 and 60min after administration of cortisone, protein synthesis in the liver increased significantly, and heavy polysomes were formed similar to those in mice which had not received an operation.
2. At 3, 4 or 5 hr after its administration, cortisone caused marked repression of protein synthesis in mouse liver. The repressive factor(s) was present in both the polysomal fraction and the S-105 fluid. The amount of heavy polysomes did not decrease at this stage, judging from results of sucrose gradient analysis of the polysome distribution. The repressive effect of the S-105 fluid was larger than that of the polysomal fraction and so the effect may be called a “cytoplasmic repression.”
3. The earlier and later effects and the time course of the response to cortisone were similar in adrenalectomized and untreated mice.抄録全体を表示PDF形式でダウンロード (452K) -
TOKUJI KIMURA, HIROIE OHNO1968 年 63 巻 6 号 p. 716-724
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーA highly purified non-heme iron protein has been prepared from pig testis. The protein contains iron and labile sulfide in approximately equimolar amounts. Optical absorption maxima occur at 455mμ, 415mμ, 320mμ and 276mμ with a small shoulder at 518mμ in the oxidized form. In the reduced form, the absorption in the visible region is markedly bleached and a new maximum appears at 550mμ. The optical rotatory dispersion and the circular dichroism spectra exhibit marked multiple Cotton effects. Upon reduction, the dispersion spectrum is greatly changed. The electron spin resonance spectrum exhibits a temperature sensitive signal at g=1.94 upon reduction. All of the pro-perties are strikingly similar to those of adrenal non-heme iron protein (adrenodoxin) and plant ferredoxins.
The substitution of the non-heme iron protein from testis for adreno-doxin in the adrenal steroid 11-β-hydroxylation reaction was accom-plished, and the preparation of non-heme iron protein from ovary could also be substituted for adrenodoxin, whereas the similar preparation from liver, spinach and Euglena ferredoxins, Pseudomonas rubredoxin and putida redoxin were all inactive. The absence of adrenodoxin-like non-heme iron protein in the brain, liver, kidney and pancreas is suggested.抄録全体を表示PDF形式でダウンロード (1357K) -
HIROKO TAKESADA, KOZO HAMAGUCHI1968 年 63 巻 6 号 p. 725-729
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーCircular dichroic (CD**) spectra of octopus vulgaris (Japanese form) hemocyanin were measured over a wavelength range of 210 to 700mμ in the forms of oxy-, deoxy- and apo-hemocyanin. Oxyhemocyanin showed three bands in the visible region (a positive band above 700mμ, a negative band at 570mμ, and a positive band at 450 mμ), a large negative band at 350mμ, several positive maxima in the ultraviolet region and a negative trough at 222 mp. In the CD spectra of deoxy-and apo-hemocyanin, characteristic three bands in the visible region and a large band at 350 mp completely disappeared, but the bands in the ultraviolet region still remained. A little change in the CD spectrum was observed in the ultraviolet region on oxygenation of deoxyhemocyanin.抄録全体を表示PDF形式でダウンロード (340K) -
TETSURO YOSHIMURA, AKIRA IMANISHI, TOSHIZO ISEMURA1968 年 63 巻 6 号 p. 730-738
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリー1. Poly-DL-alanyl-lysozyme (PAL) was prepared from lysozyme [EC 3. 2. 1. 17] and N-carboxy-DL-alanine anhydride, and was purified by CM-cellulose column chromatography. The effluent containing PAL was fractionated for further studies.
2. Six samples of PAL were obtained : The enrichment of these samples with alanine was in the range between 31 and 150 residues per molecule. The number of alanyl residues attached to lysozyme increases with increasing ratio of reacting alanine monomer to lysozyme. Although all of the lysyl side chains in the lysozyme molecule are exposed to the solvent, there seems to be a difference in the accessibility among the lysyl residues.
3. No conformational change, caused by the addition of polyalanyl groups, could be detected from the measurement of optical rotatory dispersion and circular dichroism.
4. The enzymatic activity of PAL toward glycol chitin decreases proportionally with the average length of the polyalanyl side chain. The formation of the ES-complex is inhibited by the polyalanyl side chains. These facts suggest that polyalanyl side chains sterically hinder the interaction of the enzyme molecule with substrate.
5. The effect of polyalanyl side chains on the lytic activity is more marked than that on the activity toward glycol chitin, and the lytic activity of PAL remarkably depends on pH. This implies that not only the active center, but also some other sites of the enzyme molecule are involved in the interaction of lysozyme with M. lysodeicticus and that the net charge affects the interaction.抄録全体を表示PDF形式でダウンロード (588K) -
VI. Effects of ATP Cocentration, pH and TemperatureHIROFUMI ONISHI, HIROSHI NAKAMURA, YUJI TONOMURA1968 年 63 巻 6 号 p. 739-752
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーAfter mixing ATP with myosin the reaction was stopped by adding 5 per cent trichloroacetic acid, and the amount of Pi liberated was measured. The change in hydrogen ion concentration was measured by a stopped-flow method at pH 8.3, in which the hydrolysis of ATP by myosin yielded 1 mole of hydrogen ion per mole of ATP hydrolyzed. The following results were obtained from this experiment.
1. At ATP concentrations lower than 1 mole per 4×105g of myosin, both the initial rapid Pi and hydrogen ion-liberations followed mono-molecular kinetics, and their rates were equal. The rates of Pi and hydrogen ion-liberation were independent of the ATP concentration when the concentration was lower than 1 mole per 4×105g of myosin, but they increased with increasing ATP concentration at concentrations above this.
2. The amount of initial rapid Pi-liberation increased linearly with the ATP concentration until the amount of added ATP reached about I mole per 4×105g of myosin. At higher ATP concentrations, the amount was constant at about 1 mole per 4×105g of myosin.
3. At ATP concentrations lower than about 1 mole per 4×105g of myosin, the amount of initial rapid hydrogen ion-liberation also increased with increasing ATP concentration, and reached about 1 mole per 4 × 105 g of myosin at ATP concentrations higher than the stoichiometric amount.
4. The pH-dependence of the rate of initial rapid P1-liberation was different from that of the rate at the steady state: the initial rate increased with increasing pH and the half maximum value was obtained at pH 6.4.
5. The activation entropy of the initial rapid Pi- and hydrogen ion-liberations was found to be +21.6 cal per mole per deg., while the activation entropy of P liberation at the steady state was -24.8 cal per mole per deg.
6. The rate of the actomyosin type ATPase [EC 3. 6. 1. 3] reaction increased with increasing pH and the half maximum value was ob-tained at pH 6.1. The activation entropy of the actomyosin type ATPase reaction was -45.5 cal per mole per deg.抄録全体を表示PDF形式でダウンロード (2830K) -
TATEO YAMANAKA, YOKO NAGATA, KAZUO OKUNUKI1968 年 63 巻 6 号 p. 753-760
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーCytochrome c-553 and cytochrome B-560 were highly purified from the protozoan, Tetrahymena pyriformis. Cytochrome c (553, T. pyriformis) possesses an absorption maximum at 410 mμ in the oxidized form, and maxima at 414, 523 and 553mμ in the reduced form. The ratio of A414 mμ(reduced)/A553mμ, (reduced) of the cytochrome is 5.35, and the mil-limolar extinction coefficient of the a-peak is 27.4. The midpoint redox potential of the cytochrome is about -1-0.25 volt at pH 7.0. The cyto-chrome c is an acidic protein on the basis of its affinity for diethyl-aminoethylcellulose and is not precipitated by saturation with am-monium sulphate. It reacted slowly with Pseudomonas cytochrome oxidase [EC 1. 9. 3. 2], but did not react with cow cytochrome oxidase [EC 1. 9. 3. 1].
Cytochrome B (560, T. pyriformis) shows an absorption peak at 411 mu in the oxidized form, and peaks at 424, 529 and 560mμ in the reduced form. This cytochrome is autoxidizable, and combines with carbon monoxide and cyanide, but not with azide, and does not show any peroxidase activity.抄録全体を表示PDF形式でダウンロード (576K) -
WITOLD DRABIKOWSKI, YOSHIAKI NONOMURA, KOSCAK MARUYAMA1968 年 63 巻 6 号 p. 761-765
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーInteraction among F-actin, 6S component of α-actinin and tropo-myosin was investigated by turbidity, viscosity and flow birefringence measurements. 6S component of α-actinin caused gelation of F-actin, eventually leading to precipitation. Tropomyosin, when added before, inhibited the precipitation of F-actin by 6S component. These results were presented in details, and possible physiological importance of the interactions among the three muscle structural proteins was discussed in relation to the structure of myofibrils.抄録全体を表示PDF形式でダウンロード (970K) -
HIROKO NISHIBAYASHI, RYO SATO1968 年 63 巻 6 号 p. 766-779
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーLiver microsomes from phenobarbital-pretreated rabbits were di-gested with Nagarse (Bacillus subtilis proteinase) anaerobically in the presence of 25% glycerol at 0°C for 15 hr. Cytochrome b5 and NADPH-specific flavoprotein were almost completely solubilized by this treatment, but P-450, the other microsomal hemoprotein, was quantitatively retained in the microsomal particles without appreciable conversion to the modi-fied P-420 state. The particles thus obtained (P-450 particles) containing P-450 as the sole protoheme constituent were suitable for measurements of absolute spectra of P-450. Using the preparation, bleached with hy-drogen peroxide, as a turbidity control, the spectra of oxidized and dithionite-reduced P-450 particles were measured. The oxidized spec-trum, having peaks at 360, 416, 535, 570 and 650mμ, was not very atypical for protoheme-containing proteins. But the reduced spectrum, with absorption maxima at 412 and 555mμ, was anomalous for hemo-proteins. The spectrum obtained on addition of CO to the reduced sample showed absorption peaks at 449 and 555mμ. Although the CO spectrum possessed a shoulder at 423mp, evidence was obtained to in-dicate that this was due to small amounts of P-420 and cytochrome b5 remaining in the preparation. Unusual interactions of ethyl isocyanide with both oxidized and reduced P-450, previously observed by difference speetrophotometry of intact microsomes, could be confirmed by measur-ing the absolute spectra of P-450 particles.抄録全体を表示PDF形式でダウンロード (1094K) -
HAJIME HIRATA, SAKUZO FUKUI1968 年 63 巻 6 号 p. 780-788
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリー1. Cytochromes in Agrobacterium tumefaciens grown on a sucrose medium were analyzed, and the presence of b-type cytochrome, cyto-chrome c552, cytochrome c556, cytochrome o and small amount of α-type cytochrome was proved.
2. By the addition of NADH2 to the sonic extract of the bacterium, both b- and c-type cytochromes were reduced, whereas by the addition of sucrose only reduction of c-type cytochrome was observed, and the participation of cytochrome b in the sucrose oxidizing system (3-keto-sucrose forming system) was eliminated.
3. From the soluble fraction of the sonic extract, cytochrome c552 and cytochrome csss were isolated. The former reacts directly with D-glucoside 3-dehydrogenase which is an FAD enzyme and catalyzes the oxidation of sucrose to 3-ketosucrose, while the latter does not react. Purified cytochrome c552 shows high affinity for the dehydrogenase: Km is 4.3 × 10-6 M.
4. Reconstitution experiment for the oxidation system of sucrose to 3-ketosucrose showed the necessity of D-glucoside 3-dehydrogenase, cytochrome c552, and the particulate fraction containing cytochrome oxidase, such as cytochrome o.
5. The main electron flow in the 3-ketosucrose forming system might be considered as follows: sucrose→ D-glucoside 3-dehydrogenase →cytochrome c552→4 terminal oxidase, O2 . Consequently, it is concluded that the 3-ketosucrose forming system has a physiological significance as an energy yielding system in Agr. tumefaciens.抄録全体を表示PDF形式でダウンロード (567K) -
HITOSHI TAKAHASHI1968 年 63 巻 6 号 p. 789-791
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (168K) -
ZIRO SUZUOKI, TAKAO MATSUO, FUMIO TOMINAGA1968 年 63 巻 6 号 p. 792-794
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (219K) -
RYUSHI NOZAWA, DEN'ICHI MIZUNO1968 年 63 巻 6 号 p. 795-797
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (146K) -
AKIRA SUZUKI, MIKI GOTO1968 年 63 巻 6 号 p. 798-801
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (240K) -
WITOLD DRABIKOWSKI, DAVID R. KOMINZ, KOSCAK MARUYAMA1968 年 63 巻 6 号 p. 802-804
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (170K) -
FUMIO WADA, ETSUKO MARUYAMA, KIYOKO SHIBAYAMA, YUKIYA SAKAMOTO1968 年 63 巻 6 号 p. 805-807
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (185K) -
TSUNEHIRO TAKANO, AKIO SUGIHARA, OSAMU ANDO, TAMAICHI ASHIDA, MASAO KA ...1968 年 63 巻 6 号 p. 808-810
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (1190K) -
ICHIO HONJO, HIDENARI TAKASAN, KAZUE OZAWA, OSAMU KITAMURA, AKIRA SAKA ...1968 年 63 巻 6 号 p. 811-813
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (835K) -
KUNIO YAGI, KENTARO OKAMURA, AKIKO TAKAI, AKIRA KOTAKI1968 年 63 巻 6 号 p. 814-815
発行日: 1968/06/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (143K)
- |<
- <
- 1
- >
- >|