巻号一覧
64 巻, 6 号
選択された号の論文の20件中1～20を表示しています
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I. Comparison of General PropertiesTOMOTAKA SHINODA1968 年 64 巻 6 号 p. 733-741
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーComparisons of general characteristics were made using six genetically different types of acid phosphatase [EC 3. 1. 3. 2, orthophosphoric monoester phosphohydrolase] from normal human red cells. Heterogeneity was observed on chromatography as well as on electrophoresis made with CM-cellulose and NaCl gradient. Acid phosphatases of phenotypes A, AB and B were found to be less stable toward heat treatment and oxidizing agents than those of AC and BC, and the difference was statistically significant. A change in electrophoretic pattern could be observed either by heat treatment or by incubation of the sample with GSSG. A new zone with slow mobility and reduced activity was formed by these treatments. The effect of guanidine on these types of acid phosphatase was tested as a function of its concentration, but no significant difference could be observed among different phenotypes of enzyme. Partial purification of the enzymes of phenotypes AB and B was also achieved.抄録全体を表示PDF形式でダウンロード (663K) -
I. Purification and Properties of Human PlasminogenYASUSHI ABIKO, MASAHIRO IWAMOTO, MASAO SHIMIZU1968 年 64 巻 6 号 p. 743-750
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーA simple method for preparation of human plasminogen of high purity from the euglobulin fraction of serum was reported. This method gave about 29-fold purification over euglobulin in about 70% yield. The preparation obtained was homogeneous in ultracentrifugal analysis, Sephadex G-150 gel filtration and disc-electrophoresis (5 hr run), but heterogenous in ion-exchange chromatography with DEAE-Sephadex A-50. The purified plasminogen was very soluble and stable in neutral salt solutions.抄録全体を表示PDF形式でダウンロード (1718K) -
II. Purification and Properties of Human PlasminYASUSHI ABIKO, MASAHIRO IWAMOTO, MASAO SHIMIZU1968 年 64 巻 6 号 p. 751-757
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーHighly purified human plasminogen was activated by urokinase to plasmin [EC 3. 4. 4. 14], which was subsequently purified by Sephadex G-150 gel filtration. Purified human plasmin was found to catalyze fibrinolysis, caseinolysis as well as hydrolysis of N-tosyl-L-arginine methyl ester. These activities were abolished by heat treatment. Purified human plasmin was inactivated much more slowly by TLCK (a chloromethylketone derivative of N-tosyl-L-lysine) than trypsin. Stability of purified plasmin was examined under various conditions.抄録全体を表示PDF形式でダウンロード (492K) -
III. Kinetics of Plasminogen Activation and Inhibition of Plasminogen-Plasmin System by some Synthetic InhibitorsMASAHIRO IWAMOTO, YASUSHI ABIKO, MASAO SHIMIZU1968 年 64 巻 6 号 p. 759-767
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリー1. The effects of synthetic inhibitors on the activation of plasminogen by streptokinase and on the esterolytic, caseinolytic and fibrinolytic activities of plasmin [EC 3. 4. 4. 14] were investigated. The inhibitors examined are ε-aminocaproic acid, trans-4-aminomethylcyclohexanecarboxylic acid, cis-4-aminomethylcyclohexanecarboxylic acid and the corresponding n-hexyl ester derivatives.
2. All of these inhibitors acted as the competitive type of inhibitor on the plasminogen activation, and the n-hexyl esters were more potent than the parent acids.
3. The acids were weak non-competitive inhibitors of the esterase activity of plasmin, while the hexyl esters were competitive inhibitors. Anti-caseinolytic effects of these synthetic inhibitors were similar to their anti-esterolytic effects. In fibrinolytic system, however, inhibitory effects of the acids were comparable with those of the esters.
4. Stereospecificity was observed in inhibitory effects of the stereoisomers of 4-aminomethylcyclohexanecarboxylic acid and its n-hexyl ester derivatives.抄録全体を表示PDF形式でダウンロード (596K) -
VII. Kinetic Studies on the Decrease in Light-Scattering of Actomyosin Induced by ATPHIROFUMI ONISHI, HIROSHI NAKAMURA, YUJI TONOMURA1968 年 64 巻 6 号 p. 769-784
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーThe time-course of decrease in light-scattering intensity of reconstituted actomyosin after adding ATP was measured by a stopped-flow method, and the following results were obtained.
1. In the presence of 1 or 2M KCl, the minimum amount of ATP required for the maximum decrease in light-scattering intensity of actomyosin was 1 mole per 4×105g of myosin. The value of τ1/2 (the time for half final decrease) was independent of the ATP concentration, when the latter was lower than the stoichiometric value (1 mole per 4×105g of myosin), but it decreased with increasing ATP concentration above the stoichiometric value. Under various conditions, the values of τ1/2 were essentially the same as those of the initial burst of hydrogen ion liberation on the addition of ATP to myosin.
2. When the concentration of myosin was varied with a fixed concentration of F-actin, the value of τ1/2 seemed to be unaffected by changing the ratio of myosin to F-actin, but the amount of ATP required for the maximum decrease in light-scattering intensity increased in proportion to the amount of myosin, and was 1 mole per 4×105g of myosin.
3. Both the amount of ATP required for the maximum decrease in light-scattering intensity of actomyosin and the amount of the initial Pi-liberation in the actomyosin-ATP system increased above the stoichiometric value, with decrease in KCl concentration below 0. 45M.
4. The rate constant of the recovery step of the decrease in lightscattering was almost five fold that of the decomposition of phosphoryl myosin into myosin+P1+H+, and about half that of the decomposition of the Michaelis complex by simple hydrolysis. The rate of the recovery phase was independent of the concentration of F-actin.
5. Both the rate of decrease and the rate of restoration of lightscattering intensity after adding ATP to actomyosin were almost independent of pH between pH 6.0 and 9.5.
6. Accordingly, the initial reaction of reconstituted actomyosin (F-A-M) with ATP (S) can be explained by the following mechanism: _??_ where F-A-M-S represents the Michaelis complex of actomyosin and ATP, _??_ is the complex of F-actin with phosphoryl myosin _??_, and M-P is a myosin-phosphate complex, which is formed from phosphoryl myosin by the liberation of ADP.抄録全体を表示PDF形式でダウンロード (1094K) -
AIKO KANEKO1968 年 64 巻 6 号 p. 785-794
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリー1. The intracellular distribution of two types of alkaline phosphatase [EC 3. 1. 3. 1] was investigated in rat liver. After biliary ligation the activity of the Mg++-insensitive alkaline phosphatase increased remarkably in the particulate fractions, whereas the Mg++-sensitive enzyme activity showed little change in the supernatant fluid.
2. The alkaline phosphatase activity in the particulate fractions was inhibited by EDTA, KCN and deoxycholate, but was not affected by NaF. The inhibition by EDTA was partially recovered by adding Mg++. The activity of Mg++-sensitive enzyme in the supernatant fluid was inhibited only by NaF.
3. Mg++ dependency of the alkaline phosphatase activity in the particulate fractions increased by dialysis or incubation, without change of the total activity which was measured in the presence of Mg++.
4. Mg++-sensitive and Mg++-insensitive alkaline phosphatases were separated by DEAE-cellulose column chromatography. Using magnesium acetate for elution, only one peak appeared in the position of the Mg++-insensitive alkaline phosphatase activity. The proportion of alkaline phosphatase activities in two peak positions varied according to the types of eluting solution, the age of enzyme preparation and temperature.抄録全体を表示PDF形式でダウンロード (648K) -
MASATAKA HIGUCHI, TOMIO OHBA, HIDEAKI SAKAI, YASUO KURASHIMA, GORO KIK ...1968 年 64 巻 6 号 p. 795-805
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーThe level of δ-aminolevulinate (ALA) synthetase in Rhodopseudomonas spheroides cells grown anaerobically in the light or grown aerobically in the dark and in which the enzyme had been induced by poor aeration decreased rapidly and extensively on aeration of the medium or addition of chloramphenicol or puromycin. The level of ALA dehydratase [EC 4. 2. 1. 24] also decreased under these conditions to a lesser extent. The ALA synthetase level in cells grown in the light also decreased under brighter light (6500 lux) even under anaerobic conditions. The extensive decrease in ALA synthetase activity could not be accounted for by its dilution due to cell proliferation, but seemed to represent actually decrease in the amount of enzyme or irreversible inactivation of the enzyme in the cells.
In contrast, the ALA synthetase level in cells grown aerobically in the dark and in which the enzyme had not been induced was not affected at all by either vigorous aeration or inhibitors of protein synthesis.
Evidence was obtained suggesting that extracts of cells grown in the light or those in which enzyme had been induced contain two types of ALA synthetase with different stabilities with respect to temperature and storage. In contrast extracts of cells grown in the dark in which enzyme has not been induced contain only one type of enzyme which is relatively stable. The levels of succinyl-CoA synthetase [EC 6. 2. 1. 4] and ribulose-1, 5-diphosphate carboxylase [EC 4. 1. 1. 39] in these cells did not decrease significantly on changing the incubation conditions or adding inhibitors of protein synthesis.
It was concluded that the ALA synthetases in cells grown in the light or in which enzyme has been induced are metabolically unstable and the stability of ALA synthetase in vivo is markedly affected by change in the intracellular oxidation-reduction state to a more oxidized state.抄録全体を表示PDF形式でダウンロード (776K) -
MUTUMI MURAMATU, SETSURO FUJII1968 年 64 巻 6 号 p. 807-814
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーVarious esters of ω-guanidino acids were prepared, and their inhibitory effects on trypsin [EC 3. 4. 4. 4], plasmin [EC 3. 4. 4. 14], thrombin [EC 3. 4. 4. 13] and plasma kallikrein [EC 3. 4. 4. 21] were studied. The protective effects of hexyl esters of various ω-guanidino acids on the inactivation of trypsin by diisopropylphosphofluoridate (DFP) were examined.
Among the hexyl esters of ε-guanidinocapric, δ-guanidinovaleric and γ-guanidinobutyric acid examined, hexyl δ-guanidinovalerate was the most effective inhibitor of tryptic and plasmin activities. The inhibitory effect of hexyl δ-guanidinovalerate on trypsin was competitive. These three hexyl esters protected trypsin from inactivation by DFP. Various esters of δ-guanidinovaleric acid caused extensive inhibition of the caseinolytic and esterolytic activities of trypsin and plasmin, and the order of their effects was hexyl>butyl>isobutyl ester. The esterolytic activity of trypsin on hexyl δ-guanidinovalerate was very small, and that of plasmin was negligible.
Hexyl δ-guanidinovalerate and ε-guanidinocaproate showed extensive inhibitory effects on the esterase activity of plasma kallikrein, although the inhibitory effect of hexyl γ-guanidinobutyrate was low. The hexyl, butyl and isobutyl esters of δ-guanidinovalerate had almost the same inhibitory effects. Hexyl δ-guanidinovalerate inhibited plasma kallikrein competitively.
Hexyl ε-aminocaproate which is a strong inhibitor of trypsin and plasmin did not inhibit the esterolytic activity of plasma kallikrein.
Hexyl δ-guanidinovalerate did not inhibit the esterolytic activity of thrombin but instead increased its activity to 120 per cent at 4mM concentration.抄録全体を表示PDF形式でダウンロード (554K) -
V. Subunits of Enzyme and Interaction with Adenosine AnaloguesSADAMASA MINATO1968 年 64 巻 6 号 p. 815-826
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーA procedure was described for the purification of adenosine deaminase [EC 3. 5. 4. 4, adenosine aminohydrolase, Aspergillus oryzae] from Takadiastase. The enzyme preparation was monodisperse as judged by ultracentrifugation and Tiselius electrophoresis. The sedimentation coefficient was 11. 9 S, and the molecular weight calculated from S20, W and D20, W was 214, 000, and from amino acid analysis 221, 000. The enzyme was dissociated into subunits by guanidine-HCI. In 2M guanidine, the enzyme showed S20, W of 8.2 and in 3M, 2.6 and 9.0. After dialysis against ammonium acetate buffer (pH 5.5) for 48 hr, the 8.2 S subunit was associated and its S20, W was 11.9. It seems that the deaminase might consist of eight subunits. Results of amino acid analysis and measurement of carbohydrate contents suggest that the deaminase is made from 75% of protein and 25% of carbohydrate. Experiments of N-bromosuccinimide inhibition indicate that the tryptophan residue may be involved in the active center of the enzyme. Although the enzyme activity was inhibited by PCMB, cysteine residue was not detected by amino acid analysis.
The most preferred substrates of the deaminase were adenosine and 5'-AMP. Moreover, ApUp, 8-azaadenosine, and formycin were also deaminated at a considerable rate. 6-Methylpurine riboside, adenosine-1-oxide, and 2-chloroadenosine did not serve as substrate, but markedly inhibited the deamination of adenosine or 5'-AMP. The structures of the substrates and the inhibitors are also discussed.抄録全体を表示PDF形式でダウンロード (2553K) -
I. Complete Digestion with Pancreatic Ribonuclease and Ribonuclease T1SHOSUKE TAKEMURA, TAKAHARU MIZUTANI, MASAZUMI MIYAZAKI1968 年 64 巻 6 号 p. 827-837
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーHighly purified tRNA1val from Torulopsis utilis, prepared by chromatography on DEAE-Sephadex columns, has been completely digested with pancreatic RNase [EC 2. 7. 7. 16] and with RNase T1 [EC 2. 7. 7. 26]. The separation and identification of the products have been carried out mainly by column chromatography. Analyses indicate that this RNA is composed of 75 nucleotide residues including 12 unusual nucleotides. This RNA contains common sequence G-T-Ψ-C- in all tRNA's of known structure, A-G-hU- common to yeast tRNA's and a probable anticodon for valine, I-A-Cp. Several long sequences have been established by overlapping of the fragments obtained from the two digests. The nucleoside adjacent to the 3'-side of the probable anticodon in this RNA has been found to be adenosine itself, while this position in most of the tRNA's of known structure is occupied by unusual nucleosides. The primary structure of this RNA has been determined after partial digestion with RNase T1, as stated in the subsequent paper.抄録全体を表示PDF形式でダウンロード (757K) -
II. Partial Digestion with Ribonuclease T1 and Derivation of the Complete SequenceTAKAHARU MIZUTANI, MASAZUMI MIYAZAKI, SHOSUKE TAKEMURA1968 年 64 巻 6 号 p. 839-848
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーThe primary structure of tRNA1val from Torulopsis utilis has been completely established by analyses of large oligonucleotides isolated from a partial digest of this RNA with RNase T1 [EC 2. 7. 7. 26]. Methods for the partial degradation of the RNA, and the isolation and sequence determination of the oligonucleotides are described, together with the derivation of the total sequence for the RNA. The established sequence can be readily arranged in the “cloverleaf” model characteristic of Saccharomyces tRNA's of known structure. Although the primary structures of the tRNA'1val from Torulopsis utilis and baker's yeast are closely similar, there are a few differences between them.抄録全体を表示PDF形式でダウンロード (669K) -
VIII. Production of Nitric Oxide by Denitrifying Reaction in the Presence of Tetramethyl-p-PhenylenediamineMACHIKO MIYATA, TAKESHI MORI1968 年 64 巻 6 号 p. 849-861
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリー1. TMPD reduces nitrite to nitric oxide through cytochrome c-552 (denitrifying bacteria) in the presence of denitrifying enzyme. The optimal pH is 6. 4-6. 6.
2. Carbon monoxide combines with the denitrifying enzyme itself and inhibits its activity; the inhibition is not relieved by light. There occurs a competition for the denitrifying enzyme between CO and NO produced by the denitrification.
3. Nitric oxide production is strongly inhibited by KCN but not by NaN3 in the presence of TMPD. Effects of various other inhibitors are also investigated.
4. By these results the possibility that NO may be an intermediate in the denitrifying reaction was reinforced.
5. A possible scheme for the process of denitrification was proposed.抄録全体を表示PDF形式でダウンロード (798K) -
IX. Nitrous Oxide, Its Production and Reduction to NitrogenTERUO MATSUBARA, TAKESHI MORI1968 年 64 巻 6 号 p. 863-871
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリー1. The role of N2O in denitrification and some characteristics of reduction of N2O to N2 in Pseudomonas denitrificans were studied, using Warburg manometry and gas chromatography.
2. Resting cells produced N2O and N2 from nitrite using lactate as hydrogen donor. The product of the initial stage of the reaction was found to be N2O, and then this N2O was reduced to N2.
3. No lag period in N2O reduction was observed using lactate as hydrogen donor and N2O as substrate. Azide, cyanide and 2, 4-dinitrophenol at low concentrations inhibited the conversion of N2O to N2. Only N2O was produced from nitrite in the presence of adequate concentrations of any of these inhibitors, indicating that N2O is probably a precursor in formation of N2 from nitrite in Ps. denitrificans.
4. The ratio of the activity for reduction of N2O to N2 to that of reduction of nitrite to N2O was about 4 at pH 7. 0, the optimum pH for N2O reduction. The apparent Michaelis constant for N2O was 3-6×10-5M.
5. N2O reduction was inhibited strongly by monoiodoacetate and CuSO4, suggesting that the enzymatic reaction involves thiol groups besides heavy metals. Carbon monoxide also inhibited 47-48 per cent of the reaction in the dark.
6. Only N2O was produced from nitrite by crude extracts prepared by sonic disruption of the cells.抄録全体を表示PDF形式でダウンロード (561K) -
YOSHIKI TAKESUE, RYO SATO1968 年 64 巻 6 号 p. 873-883
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーMucosal cells of rabbit small intestine were homogenized in 0.3M mannitol and fractionated by differential centrifugation. Sucrase [EC 3. 2. 1. 26] and alkaline phosphatase [EC 3. 1. 3. 1] were recovered mainly in the “nuclear” fraction, though considerable activities of these enzymes were also detected in the other particulate fractions. The similarity in distribution pattern indicated that the two enzymes are located in the same subcellular organelle.
Electron-transfer enzymes and hemoproteins were assayed in the mitochondrial and microsomal fractions, and it was found that the microsomal fraction contained cytochrome b5 and a CO-binding hemoprotein resembling hepatic P-450.
On subfractionation of the microsomal fraction by sucrose density gradient centrifugation, it was revealed that the sucrase activity of this fraction was associated with a structure which was different from typical microsomal vesicles and carried many small particles on its surface. The possibility is discussed that this structure was small fragments of microvilli.抄録全体を表示PDF形式でダウンロード (1534K) -
YOSHIKI TAKESUE, RYO SATO1968 年 64 巻 6 号 p. 885-893
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーThe microvillous fraction was isolated in a hypotonic EDTA medium from rabbit small-intestinal mucosal cells. It was almost free of the other subcellular membrane systems but was heavily contaminated by DNA. The DNA was separated from the microvillous membrane by sonication followed by density gradient centrifugation.
Sonically disrupted microvillous fragments which was equilibrated in 1. 5M sucrose showed high sucrase [EC 3. 2. 1. 26] activity but were low in hemoprotein content. The reverse was the case for the material recovered in the 1.3M sucrose layer. DNA was sedimented as a pellet in the density gradients employed.
Isolated microvilli stained negatively with phosphotungstate showed profiles of rod-and capsule-like structures, both of which were studded with small particles of 50 A in diameter. The membrane fragments recovered in the 1.5M sucrose layer also showed similar morphological features but the membranes floating in 1.3M sucrose did not. Possible relationship between the biochemical and morphological characteristics of isolated microvilli was discussed.抄録全体を表示PDF形式でダウンロード (1867K) -
YAHITO KOTAKE, YOSHIKO SOTOKAWA, EDAHIKO MURAKAMI, AYAKO HISATAKE, MAS ...1968 年 64 巻 6 号 p. 895-896
発行日: 1968/12/25
公開日: 2008/06/30
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KEITARO HIROMI, SÔZABURO ONO, SHUJI ITOH, TOSHIHIKO NAGAMURA1968 年 64 巻 6 号 p. 897-900
発行日: 1968/12/25
公開日: 2008/06/30
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KÔICHI HAYANO, SAKUZO FUKUI1968 年 64 巻 6 号 p. 901-903
発行日: 1968/12/25
公開日: 2008/06/30
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MAKOTO HORI, JIRO SUZUKI, HAMAO UMEZAWA1968 年 64 巻 6 号 p. 905-907
発行日: 1968/12/25
公開日: 2008/06/30
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TOMOH MASAKI, OSASI TAKAITI, SETSURO EBASHI1968 年 64 巻 6 号 p. 909-910
発行日: 1968/12/25
公開日: 2008/06/30
ジャーナル フリーPDF形式でダウンロード (740K)
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