巻号一覧
71 巻, 4 号
選択された号の論文の22件中1～22を表示しています
- |<
- <
- 1
- >
- >|
-
Shiro IKAWA, Yoshikazu AYAKI, Michio OGURA, Kazumi YAMASAKI1972 年 71 巻 4 号 p. 579-587
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリー3-Oxo-7α-hydroxychol-4-enoic acid-24-14C was effectively converted to chenodeoxy-cholic acid together with α-and β-muricholic acids by rats furnished with a bile fistula. It was also transformed to chenodeoxycholic acid by the supernatant of a rat liver homogenate fortified with NADPH. These results are discussed on the basis of the new biogenetic pathway of chenodeoxycholic acid proposed by Ayaki and Yamasaki (1).抄録全体を表示PDF形式でダウンロード (1102K) -
Takaharu MIZUTANI1972 年 71 巻 4 号 p. 589-595
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーChemical modification of pseudouridylic acid in tRNAval1 from Torulopsis utilis was studied with [14C] N-cyclohexyl-N'-β-(4-methylmorpholinium) ethylcarbodiimide iodide (CMC). After the modified tRNA having 10.6 moles of CMC, obtained from a frac-tionation on DEAE-cellulose, was incubated in 0.02M carbonate buffer (pH 10.3) for 3 hr at 37°C, the tRNA having 3 moles of CMC was obtained. The results of analyses of the tRNA having 3 moles of CMC showed that_??_in C-_??_-U-Ip, T-_??_-C-Gp, U-C-_??_-A-Gp and C-A-_??_-C-U-Gp were modified and the ratios of moles of the modified oligonucleotides to mole of tRNA were 0.45, 0.38, 0.58 and 0.53 respectively. Both tRNA's having 10.6 and 3 moles of CMC could not accept valine. Patterns of absorb-ance-temperature profile and ORD of the tRNA having 3 moles of CMC partially resembled to that of native tRNA in the same phosphate buffer. But the patterns of the tRNA having 10.6 moles of CMC suggested the destraction of the conformation of the tRNA.抄録全体を表示PDF形式でダウンロード (537K) -
I. Changes in the Intensity and Polarization of the Intrinsic Fluorescence (Tyrosine)Akiyoshi SATOH, Koshin MIHASHI1972 年 71 巻 4 号 p. 597-605
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーChanges in the intensity and the polarization of intrinsic fluorescence (tyrosine) of rabbit skeletal tropomyosin were examined as a function of temperature. An anoma-lous thermal quenching was observed at around 34°C in neural salt solution. This was independent of the degree of polymerization of tropomyosin. At the same tem-perature, another type of anomalous behavior of the fluorescence intensity (increase) was observed both in 5M urea where most of the α-helical structure was lost, and at pH 2 where the α-helical structure of the protein was very stable. These results suggested the precence of a small non-helical structure in tropomyosin. Judging from a polarization study, the tyrosine side-chain groups of tropomyosin seemed to be tightly incorporated in the α-helical structure in neutral salt solution. When the temperature was raised an abrupt depolarization was observed at 34°C and at 54°C. The latter corresponded to thermal melting of the α-helix of the protein. Isothermal measurements showed that the abrupt depolarization at 34°C was due to an enhanced thermal activation of the rotation of tyrosine residues. The fraction of the activated residue was estimated to be about 20% of total tyrosine residues.抄録全体を表示PDF形式でダウンロード (783K) -
II. Fluorescence Depolarization of Tropomyosin-Fluorescein Isothiocyanate Conjugate; A Change in the Rotational Relaxation Time Near Physiological TemperatureKoshin MIHASHI1972 年 71 巻 4 号 p. 607-614
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーRabbit skeletal tropomyosin was conjugated with fluorescein isothiocyanate at neutral pH and the depolarization of the fluorescence was measured as a function of tem-perature. Rotational relaxation times determined from isothermal experiment at room temperature were from 30 to 45 nanoseconds varing with preparation. Since the rotational relaxation time of a sphere equivalent to tropomyosin monomer is of the order of 120 nanosecond, the above value indicates a flexibility or a segmental motion of tropomyosin. When temperature was raised, a remarkable depolarization was observed around 33°C (at pH 7.5) and the rotational relaxation time reduced to the order of 10-20 nanoseconds. Concomitant with the depolarization, the intrinsic viscosity changed from 0.45dl/g to 0.04dl/g indicating a gross conformational change. This conformational state of low intrinsic viscosity was stable up to 45°C and was completely reversed when temperature was lowered again.抄録全体を表示PDF形式でダウンロード (699K) -
Shogo ICHII, Akemi IKEDA, Akiko OHTA, Masao IZAWA1972 年 71 巻 4 号 p. 615-623
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリー1. Protein and phospholipid contents were determined in rat adrenal subcellular fractions during the course of tissue growth induced by successive administration of ACTH. Wet weight of the tissue was increased 5 times over that of the control, while increases in protein and phospholipid were 230 and 70% over the respective control after 10 days of the treatment. Proteins in the post-mitochondrial fraction and phospholids in the crude nuclear fraction showed a most profound increase.
2. Phospholipid responded to ACTH promptly and reached the almost maximum level within a day after start of the treatment, while the content of protein increased gradually during the experimental period.
3. Concentrations of plasma corticosterone in ACTH-administered animals was sus-tained at a high level and remained almost unchanged throughout the experimental period.
4. Simultaneous labeling of protein with 3H-leucine and of phospholipid with 3H-choline was performed to examine short-and long-term effects of ACTH on the biosynthesis of these compounds. At 3.5hr postinjection of ACTH, increased protein synthesis was observed only in the inner membrane and matrix fraction of mito-chondria, while, the rate of incorporation of 3H-choline into phospholipid was stim-ulated in every subcellular fractions. After 3 days of consecutive ACTH administra-tion, the rate of protein labeling was significantly elevated, while the rate of 3H-choline incorporation returned to the control level.
5. Phospholipid content was markedly increased in the outer membrane fraction of mitochondria but the increase in phospholipid of the other subcellular fractions were quite moderate at 3 days after the start of the treatment.
6. Half-life of protein and phospholipid in the adrenal was enlengthened by the administration of ACTH.
7. Administration of cycloheximide prior to ACTH injection abolished the effect of the hormone on the both corticosterone production and the rate of 3H-choline incorpo-ration into phospholipids of the adrenal. Similarities were obtained between responses of steroid hormone production and of phospholipid biosynthesis to ACTH and cyclo-heximide.
8. Physiological implications of these observations were discussed.抄録全体を表示PDF形式でダウンロード (842K) -
Shigeru KUROOKA, Kanoo HOSOKI, Yoshio YOSHIMURA1972 年 71 巻 4 号 p. 625-634
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーLong fatty acyl-Coenzyme A thioesterase [EC 3. 1. 2. 2] is widely distributed in mam-malian tissues. The enzyme activity (per g wet weight of tissue) in rats was found to be 8 to 10 times higher in brain and testis than in liver, kidney, heart or blood cells (erythrocytes. The enzyme was purified 40 to 60 fold from these tissues and the substrate specificities of preparations from the various tissues were compared using C6-, to C18-acyl-Coenzyme A thioesters. The enzyme hydrolyzed C8 to C18 thio-esters, C14 and C16 thioesters being the best substrates, with Km values 3 to 5×10-6M. Preparations from the various tissues had similar substrate specificities. Moreover the molecular weights of enzymes from these tissues were all estimated to be about 50, 000, by sucrose density gradient centrifugation. However, the gel filtration pat-terns of the enzymes from different tissues were not similar and the patterns were classified into three types: 1) liver type, 2) heart, kidney, and spleen type, and 3) brain, testis and blood cell type.
The enzyme activity was detected in blood plasma in rats intoxicated with CCl4 and the gel filtration pattern showed that this enzyme was released into the blood plasma only from the cytoplasm of the liver.抄録全体を表示PDF形式でダウンロード (797K) -
Masanobu JANADO, John DUNSTONE1972 年 71 巻 4 号 p. 635-643
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーA glycoprotein has been isolated from bovine nasal cartilage by various methods and its chemical and physicochemical properties were examined.
1. Analytical disc electrophoresis on acrylamide gel shows that, besides the major component, a number of minor components are also present.
2. The relative proportions of these minor fractions increase with time. The presence of large amounts of half-cystine residues in the peptide portion suggests that the minor fractions could be formed from the main component by rearrangements in-volving disulphide bonds. This tentative suggestion still requires proof.
3. The glycoprotein contains approximately 92% of protein, 3% hexosamine, 1% uronic acid and 2% neutral sugars. The uronic acid component appears to be part of a glycosaminoglycuronoglycan structure which is an integral part of the glyco-protein molecule.
4. The glycoprotein has a probable molecular weight near 45, 000.抄録全体を表示PDF形式でダウンロード (2692K) -
Hidekazu IWASAKI, Teruo MATSUBARA1972 年 71 巻 4 号 p. 645-652
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーA nitrite reductase [EC 1. 7. 99. 3] was isolated from Achromobacter cycloclastes and characterized. The oxidized form of the enzyme had absorption maxima at 283, _??_400 (a shoulder), 464, 590 and 700mμ; the reduced form had no peak in the 400-800mμ region. The molecular weight was 69, 000. It had two copper atoms per molecule.
The enzyme catalyzed nitric oxide production from nitrite, whereby most part of the nitrite-nitrogen was converted to nitric oxide gas. Optimum pH of this reac-tion was 6.2. Km for nitrite was estimated to be _??_5×10-4M. This reaction was inhibited by KCN, diethyldithiocarbamate, p-chloromercuribenzoate and CO.
Nitrous oxide production from nitrite and hydroxylamine was also catalyzed by the enzyme.抄録全体を表示PDF形式でダウンロード (1005K) -
IV. Substrate Specificity of Aspergillus carneus DextranaseDaisuke TSURU, Nobutsugu HIRAOKA, Juichiro FUKUMOTO1972 年 71 巻 4 号 p. 653-660
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーSubstrate specificity of the dextranase [α-1, 6-glucan 6-glucanohydrolase, EC 3. 2. 1. 11] of Aspergillus carneus was investigated using a series of isomaltodextrins, dextran and their derivatives as the substrates. The enzyme readily hydrolyzed dextran T-2000 which consisted of more than 95% of α-1, 6-glucosidic linkage, giving rise to isomaltotriose, isomaltose and a small amount of glucose together with traces of higher oligomers, the degree of hydrolysis being about 40%. Dextran IAM that contained 66% of α-l, 6-glucosidic linkage was hydrolyzed by the enzyme very slowly and to a lesser extent. Among a series of isomaltodextrins tested, G4 to G8 com-pounds were split into isomaltose and isomaltotriose at a rate comparable with that of hydrolysis of dextran T-2000. The enzyme attacked isomaltotriose at an extremely slow rate and did not act on isomaltose. Comparison of the enzymatic digestion products from isomaltodextrins and their reduced derivatives (sugar alcohols) suggests that the enzyme removes primarily isomaltotriosyl unit and, to somewhat lesser extent, isomaltosyl unit from the nonreducing end of isomaltodextrins. Synthetic reaction was observed by incubation of the enzyme with high concentration of iso-maltose and of isomaltotriose but not from glucose.抄録全体を表示PDF形式でダウンロード (1806K) -
II. Effect of Thermal Treatment on Light MeromyosinsKunihiko SAMEJIMA, Jun-ichiro MORITA, Koui TAKAHASHI, Tsutomu YASUI1972 年 71 巻 4 号 p. 661-673
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーA new form of light meromyosin was prepared according to the method described by Young et al. (1). Thermostability of this protein (which they called LMM-C) was studied in comparison with tryptic light meromyosin fraction 1 (LMM Fr 1). Differences between LMM Fr 1 and LMM-C upon heating can be detected by solu-bility, viscosity, optical dispersion, difference spectrum (at U. V. region) and mor-phological studies. Upon thermal treatment, LMM Fr 1 depolymerizes to relatively low molecular weight proteins and peptides. The existence of a “temperature opti-mum” (60°C being more effective than 70°C) can be clearly observed. LMM-C, on the other hand, does not depolymerize, suggesting that it is more stable than LMM Fr 1 at high temperatures (45-60°C) under our experimental conditions. Addition of trypsin-trypsin inhibitor complex during the course of preparation of LMM-C and myosin converts the properties of the proteins to those of tryptic LMNI Fr 1, as far as their thermostability is concerned. Our experiments reported here seem to give full support to the view that protomyosins are formed upon thermal treatment by proteolytic contaminations, if they are present. We have considered whether LMM-C might represent a more natural form of α-rope section of myosin.抄録全体を表示PDF形式でダウンロード (3835K) -
Koko MURAKAMI, Sadahiko ISHIBASHI1972 年 71 巻 4 号 p. 675-684
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーThe regulatory mechanism for the three low Km hexokinase [EC 2. 7. 1. 1] isozymes was studied with carrageenin-induced proliferative granuloma tissue of rats in which hexokinase activity per g of tissue was much higher as compared with that in the liver and muscle.
The electrophorsis of hexokinase isozymes on cellulose acetate membrane revealed that the granuloma contained Types I, II and III, and their relative proportions were altered corresponding to the change in the proliferative activity of the tissue. Only Type III was detected first, which was followed by the appearance of Type I. Then, gradual replacement of Type III by Type II was observed.
Possible role of insulin in the regulation of the hexokinase isozyme pattern was examined. In the absence of mercaptoethanol (ME) and EDTA, administration of insulin caused increase in Type III and concomitant decrease in Type II in the granuloma, seemingly converting Type II to III. This change was apparenty inhibited by puromycin and actinomycin D administration. In the liver and muscle, the insulin treatment caused an almost disappearance of Type II without appreciable change in Type III. In the muscle, the change in the isozymogram was seen as early as 5 min after the insulin injection. The effects of insulin, however, were not found in the presence of ME and EDTA during the preparation and analytical procedures.抄録全体を表示PDF形式でダウンロード (3976K) -
V. Photochemical Reaction and Photoproducts of 5'-AMPKihachiro UEHARA, Takao HAYAKAWA1972 年 71 巻 4 号 p. 685-697
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーThe photodynamic breakdown of adenosine 5'-monophosphate (5'-AMP) by riboflavin was investigated. In the presence of oxygen and riboflavin and in the light, a reac-tion of 5'-AMP leading to a decrease in ultraviolet absorption accompanied by the production of a considerable amount of a volatile basic material which seemed to be ammonia occurred. At least seventeen photoproducts have been separated by means of Dowex 1-formate column chromatography. Two products of the initial stage of the reaction was identified to be inosine 5'-monophosphate (5'-IMP) and 8-oxyadenosine 5'-monophosphate (8-Oxy-AMP). Ribose 5-phosphate and both allantoin-and parabanic acid-like mononucleotide were also found among the products. The significance of these findings in relation to the mechanism of photodegradation of adenine-containing nucleotides and to the specificity of riboflavin as a sensitizer for the photoreaction have been discussed.抄録全体を表示PDF形式でダウンロード (1207K) -
Haruhiko NODA1972 年 71 巻 4 号 p. 699-703
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーPartial specific volume of collagen in aqueous solution is 0.70ml. There is little change of volume upon denaturation of collagen molecules. In concentrated salt solutions, the partial specific volume shows apparent increase.抄録全体を表示PDF形式でダウンロード (955K) -
II. Influence of the Fatty Acid Content on the Conformation of Bovine Serum Albumin MonomersFrederik SOETEWEY, Maryvonne ROSSENEU-MOTREFF, Roger LAMOTE, Hubert PE ...1972 年 71 巻 4 号 p. 705-710
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーThe fatty acid content of bovine serum albumin (BSA) monomer samples was altered either through delipidation or relipidation with long-chain fatty acids. The investiga-tion of the dielectric properties and of the viscosity of the solutions enables the study of the induced conformational changes.
Relipidated BSA is characterized by a higher mobility on agar gel electrophoresis than the native and delipidated samples. The relipidated BSA molecule becomes more spherical, its equivalent volume increases and its dipole moment decreases through fatty acid addition, whereas its intrinsic viscosity remains unchanged.抄録全体を表示PDF形式でダウンロード (1169K) -
Yoshimi MATSUMURA, Toshiharu MATSUMURA1972 年 71 巻 4 号 p. 711-717
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーMyosin ATPase [ATP phosphohydrolase, EC 3. 6. 1. 3] from rabbit skeletal muscle was inactivated by quartz, mica, talc and other silicate minerals which were added to the incubation medium for the enzymatic reaction. The extent of ATPase inac-tivation by these condensed polysilicic acids was correlated to the amounts of ex-changeable hydrogen on the surface, to the surface area and to the species of poly-silicic acids. The powders of mica, talc and quartz inactivated ATPase more intensely than those of rhodonite, sillimanite and diopside in an equal amount. It was shown that the surface of the polysilicic acid powder for adsorption of myosin rather than the silicic acids dissolved in the medium is responsible for the ATPase inactivation. The inactivation was discussed in relation to the surface area, the ion-exchange capacity and the crystal structure of the polysilicic acids.抄録全体を表示PDF形式でダウンロード (563K) -
Yoshiyuki KUCHINO, Hideya ENDO1972 年 71 巻 4 号 p. 719-723
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーAn inhibitor for tRNA methylase was isolated and crystallized from the high speed supernatant fraction of rat liver by Dowex-50 (H+) column chromatography, and was identified as nicotinamide. Another nondialyzable protein factor was, however, shown to be required for the demonstration of the inhibitory action of nicotinamide. This factor was tentatively identified as nicotinamide methyltransferase [EC 2. 1. 1. 1]. Kinetic analyses indicated that the inhibitory action of both factors on tRNA meth-ylase appeared to be attributed to the competition of tRNA and nicotinamide transmethylases for a common substrate, S-adenosylmethionine.抄録全体を表示PDF形式でダウンロード (406K) -
Katsuyoshi MIHARA, Ryo SATO1972 年 71 巻 4 号 p. 725-735
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーNADH-Cytochrome b5 reductase [EC 1. 6. 2. 2] (fp1) was solubilized from rabbit liver microsomes with Triton X-100 and sodium deoxycholate and purified 30-fold in the presence of Triton X-100. This preparation was called “detergent-solubilized fp1” or simply “d-fp1” to distinguish it from the fp1 preparation purified after solubilization by lysosomal digestion (“lysosome-solubilized fp1” or “1-fp1”). The d-fp1 preparation still contained phospholipids and was contaminated by cytochromes P-450 and P-420, but was free from cytochrome b5 and NADPH-cytochrome c reductase system. A considerable amount of non-heme iron, showing an electron spin resonance (ESR) signal at g=4.3, was also present in the preparation, but this iron did not seem to be functional in the catalysis.
When the preparation was subjected to Sephadex gel filtration in the presence of 1.0% deoxycholate, the reductase activity was eluted at a position corresponding to a molecular weight of 45, 000. After treatment with lysosomes or partially puri-fied cathepsin D, the elution volume of the reductase activity corresponded to a smaller molecular weight of about 28, 000, which was similar to the value reported for 1-fp1.
d-fp1 could react with cytochrome b5 purified using detergent (“d-b5”) as well as with that solubilized by trypsin (“t-b5”) to reconstitute the NADH-cytochrome c reductase activity. However, it interacted with d-b5 much better than with t-b5. The highly efficient interaction with d-b5 was abolished when d-fp1 was digested with lysosomes or cathepsin D, though the reactivity of d-fp1 with t-b5 was unaffected by these treatments. It is suggested that the fp1 preparations obtained previously (1-fp1) are functionally incomplete.抄録全体を表示PDF形式でダウンロード (1046K) -
Satoshi TOKUTAKE, Tuneo OKUYAMA1972 年 71 巻 4 号 p. 737-741
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (321K) -
The Difference in the Availabilities of Initiation Points of Phage RNA Cistrons in Their TranslationYoshimi OTA, Takahisa OHTA, Kazutomo IMAHORI1972 年 71 巻 4 号 p. 743-746
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (358K) -
Teruo MATSUBARA, Hidekazu IWASAKI1972 年 71 巻 4 号 p. 747-750
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (981K) -
Detection of Arginine Esterase of Various Kallikrein Preparations on Gellified Electrophoretic MediaYukio FUJIMOTO, Hiroshi MORIYA, Keiko YAMAGUCHI, Chiaki MORIWAKI1972 年 71 巻 4 号 p. 751-754
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (1050K) -
Tomoh MASAKI, Chiho YOSHIZAKI1972 年 71 巻 4 号 p. 755-757
発行日: 1972/04/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (251K)
- |<
- <
- 1
- >
- >|