巻号一覧
72 巻, 4 号
選択された号の論文の29件中1～29を表示しています
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Tamotsu TAKETOMI, Nariko KAWAMURA1972 年 72 巻 4 号 p. 799-806
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーIt was found that glycolipids of caprine erythrocyte consisted of predominantly Forssman hapten active globoside and small amounts of glucosyl-ceramide, lactosyl-ceramide, digalactosylglucosyl-ceramide, globoside I, and galactosyl-ceramide. Investigation of products of partial hydrolysis, and immunochemical analysis of caprine erythrocyte globoside I and Forssman globoside indicated that globoside I might be a precursor of Forssman globoside. These minor and major glycolipids as well as sphingomyelin except galactosyl-ceramide contained normal fatty acids. Hydroxy fatty acids and phytosphingosine were absent in the caprine erythrocyte glycolipids contrary to the porcine erythrocyte glycolipids.抄録全体を表示PDF形式でダウンロード (3637K) -
Terumi SAITO, Kenkichi TOMITA1972 年 72 巻 4 号 p. 807-815
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーBeef and rat heart mitochondria contained an appreciable amount of malic enzyme activity. In contrast to lipogenic tissues such as liver and adipose tissues, the malic enzyme activity in rat heart was not influenced by feeding the animals on diets containing thyroid or a high content of carbohydrate or both.
The extra- and intra-mitochondrial malic enzymes [EC 1. 1. 1. 40] of heart wen different in several respects such as chromatographic behavior on DEAE-cellulose and Sephadex G-200, Km values for malate, the extent of inhibition by dicoumarol, the postnatal alteration in content, and the immunological reaction with the antiseruir to purified malic enzyme of liver.
The beef heart cytosol malic enzyme was separated on DEAE-cellulose into twc fractions, cytosol I and cytosol II, the latter was not distinguishable from the mitochondrial malic enzyme on this column. However, cytosol II had a smaller molecular weight than the mitochondrial enzyme as determined by gel filtration on Sephadex G-200.
The intramitochondrial distribution study of beef heart malic enzyme using the digitonin method indicated that the enzyme was fairly tightly bound to the innei membrane of the particles.抄録全体を表示PDF形式でダウンロード (635K) -
Yoichi YAZAWA, Koichi YAGI1972 年 72 巻 4 号 p. 817-826
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリー1. A correlation between elution volume of a solute in gel nitration and molecular weight of the solute was investigated in 5M guanidine hydrochloride by using proteins of known molecular weight. Sepharose 4B, Sepharose 6B, Sephadex G-200, and Sephadex G-100 were used in the medium of 5M guanidine hydrochloride, ImM EDTA, and 20mM Tris-HCl buffer (pH 7.6) with or without β-mercaptoethanol. A plot of log molecular weight versus elution volume yields a linear relation with Sepharose 6B, Sephadex G-200, and Sephadex G-100, from which molecular weights can be estimated with an uncentainty of approximately 11-14 percent.
2. Subunit structures of myosin, heavymeromyosin, lightmeromyosin-fraction 1, and actin were examined by the method using gel nitration described above. Molecular weights of the constitutive polypeptide chains were estimated and difference between
heavymeromyosins prepared by tryptic and chymotryptic digestions was observed.抄録全体を表示PDF形式でダウンロード (719K) -
II. Digestion of the CsCl Treated Ribosomes With RNase T1Haruo KAGAWA, Hiroaki TOKIMATSU, L. JISHUKEN1972 年 72 巻 4 号 p. 827-831
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーThe 50S subunits or the CsCl-treated 50S subunits of E. coli Q13 were digested with RNase T1 [EC 2. 7. 7. 26] in various conditions. The digests were fractionated by the method of the “successive” polyacrylamide gel electrophoresis. From these experiments we tried to clarify the relative neighboring positions of the 50S proteins on polynucleotide fragments and estimate a possible topographical arrangement of SOS protpins on the 23S rihosomal RNA.抄録全体を表示PDF形式でダウンロード (1240K) -
Haruo SUZUKI, Yasuyuki OGURA1972 年 72 巻 4 号 p. 833-839
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーThe inhibitory effect of 8-hydroxyquinoline on the amine oxidase [monoamine: oxygen oxidoreductase (deaminating), EC 1. 4. 3. 4] from Aspergillus niger was analyzed on the basis of data which were obtained by the overall reaction kinetics and also by the “flow” method. It was confirmed that 8-hydroxyquinoline inhibited the transamination step non-competitively, but did not inhibit the oxidative reaction of the pyridoxamine phosphate (PMP) form with molecular oxygen. The following reaction scheme for the mode of inhibition of the amine oxidase by 8-hydroxyquinoline was proposed:_??_
where EL stands for the pyridoxal phosphate (PLP) form of the amine oxidase (enzyme unit based on PLP), EM for the PMP form and X and Y for intermediates in the reactions. EL1 and XI are a PLP form-inhibitor complex and an intermediate (X)-inhibitor complex(es), ** respectively.抄録全体を表示PDF形式でダウンロード (504K) -
II. A Bitter Peptide Consisting of Tryptophan and LeucineNoshi MINAMIURA, Yoshikazu MATSUMURA, Takehiko YAMAMOTO1972 年 72 巻 4 号 p. 841-848
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーFrom the cow milk casein digests with alkaline proteinase of Bacillus subtilis was isolated a bitter peptide consisting of only tryptophan and leucine which was soluble in chloroform, but sparingly soluble in water. The peptide was attacked neither by aminopeptidase nor carboxypeptidase and showed no electrophoretic mobility. The peptide was negative in detection tests for amino and carboxyl groups. A partial hydrolysis by acid resulted in loss of bitterness and the hydrolysates became positive in the tests for amino and carboxyl groups.
Amino acid analysis revealed that the peptide was composed of equimolar tryptophan and leucine, and a cyclic structure consisting of two moles of tryptophylleucine was suggested for the bitter peptide.抄録全体を表示PDF形式でダウンロード (2308K) -
Takeshi SHIGA, Yoshihiro UEDA, Itiro TYUMA1972 年 72 巻 4 号 p. 849-852
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーN-l-Oxyl-2, 2, 6, 6-tetramethyl-4-piperidinyl-iodoacetamide spin-labeled human adult hemoglobin has been combined with O2, CO, and various nitrosobenzene derivatives and its electron paramagnetic resonance spectrum has been measured at different temperatures. Compared with those of O2- and CO-liganded form, the weakly immobilized signal of the hemoglobin liganded with nitrosobenzene derivatives was narrow and, moreover, the intensity of the signal relative to that of the strongly immobilized one was low in the perfluoro-nitrosobenzene-liganded hemoglobin. This indicates that the conformation of hemoglobin near the label binding site depends upon the size of ligands attached to hemes. The ligand-size dependent conformational difference cannot be observed if the iodoacetamide group in the label is replaced by ethylmaleimide.抄録全体を表示PDF形式でダウンロード (284K) -
I. Crystalline Factor GYoshito KAZIRO, Noriko INOUE-YOKOSAWA, Masao KAWAKITA1972 年 72 巻 4 号 p. 853-863
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーThe ribosome-dependent guanosine triphosphatase (Elongation Factor G) has been purified from Escherichia coli by protamine precipitation, ammonium sulfate fractionation, DEAE-Sephadex column chromatography, gel filtration through Sephadex G-200, and second DEAE-Sephadex column chromatography. The purified factor G was crystallized in the form of needles by addition of ammonium sulfate. The crystalline factor G appears to be homogeneous as judged from ultracentrifugation and gel electrophoresis. The sedimentation coefficient of factor G is 4.5S (so20, W) and the molecular weight as determined from the equilibrium centrifugation is 83, 000.
Factor G catalyzes the ribosome-dependent hydrolysis of GTP in the absence of messenger RNA and amino-acyl-tRNA (the uncoupled GTPase reaction). Some properties of the uncouDled GTPase reaction are described.抄録全体を表示PDF形式でダウンロード (1710K) -
II. Phenotypic Expression of Allotypic Specificities in Rabbits Exposed in Neonatal Life to Antibodies Directed against Aa1 Allotypic Determinants Specific for IgGAkira SETO1972 年 72 巻 4 号 p. 865-878
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーNeonatal rabbits were treated with antibodies directed against Aa1 allotypic determinants specific for IgG and its effects on development of allotypically determined immunoglobulins were analysed in sera of the treated animals. The treatment of Aa1 Aa3 heterozygous rabbits resulted in a delayed appearance in serum of all or some Aa1+ immunoglobulins: In some animals neither Aa1+ IgG nor Aa1+ IgM were detected for 9 weeks after birth and in others no Aa1+ IgG only Aa1 IgM was found up to 5 weeks after birth. In Aa1 Aa1 homozygous rabbits, no similar effect was observed. Another effect of the treatment was manifested as supernormal levels of serum IgG and IgM with a maximum at 9 weeks. This effect was more remarkable in homozygotes than in heterozygotes in which phenotypic expression of some Aa1 allotypic patterns was suppressed. In other heterozygotes in which phenotypic expression of all Aa1 allotypic patterns was suppressed, IgG levels were lower than controls, suggesting that the suppressed phenotypic expression was not compensated. Quantitative analyses of IgG molecules carrying Aa1 allotypic specificity have revealed that approx. only 40% of IgG molecules carried the Aa1 allotypic determinants specific for IgG in an IgG preparation from an Aa1 Aa1 homozygote in which more than 60% of the molecules possessed Aa1 allotypic specificity.抄録全体を表示PDF形式でダウンロード (2619K) -
XI. Purification and Some Properties of Sulfite Reductase, DesulfoviridinKunihiko KOBAYASHI, Eiji TAKAHASHI, Makoto ISHIMOTO1972 年 72 巻 4 号 p. 879-887
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリー1) Sulfite reductase was purified to an ultracentrifugally and disc-electrophoretically homogeneous state from a dissimilatory sulfate-reducer, Desulfovibrio vulgaris.
2) The purified enzyme had an absorption spectrum identical with that of a green pigment, desulfoviridine, showing absorption maxima at 630, 585, 410, 390, and 280mμ. The spectrum was not affected by sodium dithionite. Similarities in the spectrum and the behavior during purification indicate the identity of the enzyme with desulfoviridin.
3) From the results of sodium dodecylsulfate-polyacrylamide gel electrophoresis, it was shown that the enzyme was composed of at least two species of polypeptide chains having molecular weights of about 45, 000 and 55, 000.
4) When coupled with the hydrogenase-methylviologen system, the purified enzyme formed trithionate as well as thiosulfate and hydrogen sulfide from sulfite with the consumption of molecular hydrogen.
5) The pathway of sulfite reduction and the participation of trithionate and thiosulfate reductases in the sulfite-reducing system of sulfate-reducing bacteria, leading to the formation of sulfide, are discussed.抄録全体を表示PDF形式でダウンロード (1534K) -
I. Tryptic DigestionTadashi IMAGAWA, Akira TSUGITA1972 年 72 巻 4 号 p. 889-910
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーThe sulfate binding protein from Salmonella typhimurium was purified on a large scale essentially by the method of Pardee.
A total of 38 peptides were isolated from a tryptic digest of the heat-denatured sulfate binding protein using column and paper chromatographies and/or paper electrophoresis.
The amino acid composition and sequence of each purified peptide were determined. Six of these tryptic peptides seemed to be by-products due to the chymotryptic activity remaining in the trypsin [EC 3. 4. 4. 4] preparation after TPCK-treatment.
The sum of the amino acids in the tryptic peptide, excluding overlapping sequences, accounted for 212 amino acid residues.抄録全体を表示PDF形式でダウンロード (1426K) -
II. Thermolysin DigestionTadashi IMAGAWA1972 年 72 巻 4 号 p. 911-925
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーA total of 41 peptides were isolated from a thermolysin digest of the sulfate binding protein after heat denaturation. They were separated by column chromatography and then purified by paper electrophoresis and/or paper chromatography. The amino acid composition and complete or partial sequence of each purified peptide were analyzed. The sum of the amino acids in the thermolysin peptides, excluding possible overlapping sequences, accounted for 233 residues. All the peptides obtained by thermolysin digestion, except Lys-Asp-Ile-Gln-Leu, had either NH2-terminal leucine, isoleucine, valine, alanine, phenylalanine, tyrosine, or histidine. These residues are consistent with the specificity of this enzyme, and consequently the exceptional sequence, Lys-Asp-Ile-Gln-Leu may be the NH2-terminal sequence of the sulfate binding protein. Fifteen of the thermolysin peptides and 16 tryptic peptides could be arranged into 7 unique sequences, by overlapping sequences.抄録全体を表示PDF形式でダウンロード (908K) -
III. Digestions with Pepsin and Dilute Hydrochloric AcidTadashi IMAGAWA, Sadao SUZUKI, Akira TSUGITA1972 年 72 巻 4 号 p. 927-949
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーThe sulfate binding protein was digested with pepsin [EC 3. 4. 4. 1] after heat denaturation and also hydrolyzed directly with dilute hydrochloric acid. The resulting peptides were fractionated by Dowex 50-X2 column chromatography, and then fractionated or purified by paper chromatography and/or electrophoresis.
The peptic digestion gave 38 peptides, and their complete or partial structures were determined. From the specificity of pepsin, the COOH-terminal sequence of the protein seemed to be Asp-Glu-Ile-Ser-Lys-Arg.
After hydrolysis with dilute acid, 10 peptides were isolated in addition to free aspartic acid, pyrrolidonecarboxylic acid, tyrosine, lysine, and arginine and their structures were analyzed.
From the information derived from the peptic peptides and the peptides obtained by hydrolysis with dilute acid, together with information from thermolytic peptides 2 tryptic peptides could be constituted into 9 large unique sequences.抄録全体を表示PDF形式でダウンロード (1387K) -
III. Isolation and Partial Characterization of an Enzyme-bound PigmentKenzaburo MATSUDA, Takao NAKAMURA1972 年 72 巻 4 号 p. 951-955
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーA new flavin derivative (p-flavin) was isolated from a bacterial luciferase from Photobacterium phosphoreum. It contains isoalloxazine nucleus and phosphoric acid in a ratio of 1:4, but is more hydrophobia than FMN or riboflavin and is soluble in chloroform. The chromatographic characteristics of this new flavin do not correspond to those of any previously known flavin derivatives. Its absorption maxima are at 224, 270, 385, and 445nm with a shoulder at 470nm, and its fluorescence maximum (absolute spectrum) is at 544nm, in aqueous medium at pH 7.0. One molecule of native luciferase was found to contain an average of 0.19 molecule of this flavin, bound non-covalently.抄録全体を表示PDF形式でダウンロード (375K) -
Munekazu SHIGEKAWA, Yuji TONOMURA1972 年 72 巻 4 号 p. 957-971
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーA protein factor, which inhibited the Mg2+-activated ATPase [EC 3. 6. 1. 3] activity of actomyosin irrespective of the Ca2+ concentration, was isolated from “room-temperature” actin, which had been polymerized with O.1M KC1 and treated with 1/200 (W/W) of trypsin [EC 3. 4. 4. 4] at 25°C for 3-4min. The minimum amount of the most effective preparation of the inhibitory factor required for reduction of the acto myosin ATPase to that of myosin was half the amount of actin present in the reaction mixture. The ATPase activity of myosin was not affected by the factor.
Purified actin, tropomyosin, and troponin were treated with trypsin and then their inhibitory effects on the ATPase activity of actomyosin were tested. After trypsin-treatment only troponin was strongly inhibitory and was effective both in the presence and absence of Ca2+.
An inhibitory component with a molecular weight of 23, 000 was isolated from purified troponin. On treatment with 1/300 (W/W) of trypsin at 25°C for 3-5min, it was degraded to a subfragment with a molecular weight of 11, 000 which still had biological activity.
In the presence of tropomyosin, Ca2+ -sensitivity of the Mg2+-activated ATPase of actomyosin was reversed by combination of the two components of troponin with molecular weights of 23, 000 and 19, 000 at a weight ratio of 1:4. The reversed Ca2+ -sensitivity of the ATPase of actomyosin could also be conferred occasionally by a combination of the inhibitory factor isolated from “room-temperature” actin after trypsin-treatment and the two components of troponin with molecular weights of 37, 000 and 19, 000 at an equal weight ratio.抄録全体を表示PDF形式でダウンロード (958K) -
V. Competence of Calcium-treated CellsAkira TAKETO1972 年 72 巻 4 号 p. 973-979
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーUpon treatment with 0.05M CaCl2, cells of Escherichia coli become highly competent for transfection by replicative-form DNA of φA and φX174. The calcium-treated cells are infected by single-stranded φA DNA and also by QB RNA. Most of the cells remain viable after calcium-treatment and grow normally in the absence of any stabilizing agents. The uptake of DNA by competent cells occurred very rapidly and mature φA virus appeared within 15 to 20min after transfer to growing medium. A linear relationship was observed between the number of infective centers and the DNA challenged. Plaque yield increased proportionally with increasing cell concentrations and reached a plateau at the cell density of 3-4 O. D.660. When preserved in chilled 0.05M CaCl2, the calcium-treated cells remained competent at least for one week.抄録全体を表示PDF形式でダウンロード (494K) -
VIII. Kinetics of 2, 3-Diphosphoglycerate ChangesNaotaka HAMASAKI, Shigeki MINAKAMI1972 年 72 巻 4 号 p. 981-985
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーThe rate of increase or decrease of 2, 3-diphosphoglycerate in red cells was measured under various conditions. When the cells were incubated with carbon monoxide, the diphosphoglycerate concentration decreased with a rate of 0.9μmoles/ml cells per hr. In the presence of sodium fluoride when the glycolysis stopped completely, the concentration remains constant. The transition from anaerobic to aerobic state did not change the concentration, when preincubated with fluoride. When incubated with bisulfite, the concentration decreased with a rate of 1.6μmole/ml cells per hr and disappeared completely in 4hr. With arsenate, the concentration decreased with a rate of 0.7μmoles/ml cells per hr and with inosine and pyruvate at elevated pH, the concentration increased with a rate of 0.8μmoles/ml cells per hr and reached a value of 17mM. These rates of increase or decrease of the diphosphoglycerate concentration correspond to 25 to 50 percent of the glycolytic flow, which may indicate the maximal estimate of the diphosphoglycerate by-pass. Factors determining the diphosphoglycerate concentration in red blood cells were discussed.抄録全体を表示PDF形式でダウンロード (395K) -
Takao OKUDA, Katsuyoshi MIHARA, Ryo SATO1972 年 72 巻 4 号 p. 987-992
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーTwo types of NADH-cytochrome b5 reductase [EC 1. 6. 2. 2] (fp1) preparations, one solubilized by treatment with detergents (d-fp1) and the other solubilized by digestion with lysosomes (l-fp1), and two types of cytochrome b5 preparations, one solubilized with detergents (d-b5) and the other by tryptic digestion (t-b5), were used to study the interactions between the flavoprotein and the cytochrome. The functional interactions were studied by measuring the NADH-cytochrome c reductase activity reconstituted from the flavoprotein and the cytochrome. Among the four possible combinations tested, the system consisting of d-fp1 and d-b5 was by far the most efficient with respect to the maximal cytochrome c-reducing activity and the cytochrome b5 concentration giving half maximal activity. Gel filtration on a Sepharose 6B column indicated that a functional aggregate of large particle size was formed on mixing d-fp1 and d-55. However, no aggregate was detected when d-fp1 was mixed with t-b5 or trypsin-treated d-b5. It is concluded that aggregate formation was principally responsible for the high catalytic activity of the system consisting of d-fp1 and d-b5.抄録全体を表示PDF形式でダウンロード (481K) -
IV. A Comparative Study of the Substrate Specificities of Lysozymes from Different SourcesSaburo HARA, Yoshio MATSUSHIMA1972 年 72 巻 4 号 p. 993-1000
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーThe substrate specificities of lysozymes [EC 3. 2. 1. 17] from hen egg white, quail egg white, duck egg white, human saliva, and turnip were investigated. Reducing end groups and oligosaccharides, which were liberated by the lysozymes from bacterial cell walls, glycol chitin, and carboxymethyl-chitin, were determined. The lysozymes showed similar specificities on these substrates in which some of the hydroxy groups of N-acetylglucosamine residues are replaced by fairly bulky groups such as lactyl, hydroxyethyl, and carboxymethyl groups.抄録全体を表示PDF形式でダウンロード (565K) -
II. Purification of M2-type Pyruvate Kinase from Yoshida Ascites Hepatoma 130 Cells and Comparative Studies on the Enzymological and Immunological Properties of the Three TypesKiichi IMAMURA, Koji TANIUCHI, Takehiko TANAKA1972 年 72 巻 4 号 p. 1001-1015
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリー(1) M2-Pyruvated kinase (M2-PK) [EC 2. 7. 1. 40] was purified from a crude extract of Yoshida ascites hepatoma 130 cells by a procedure involving ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, and Sephadex G-200 column chromatography. This purified preparation appeared homogeneous on ultracentrifugation and electrophoresis, and its molecular weight was estimated to be approximately 2.16×105 by Sephadex G-200 column chromatography.
(2) Anti-M2 serum was prepared in this laboratory from the blood of a chicken immunized with purified M2-PK. Comparative studies on L, M1, and M2-PK's using anti-M1 and anti-M2 sera suggested that these three types of PK's were immunologically distinguishable.
(3) Comparative studies on the kinetic properties of the three types of PK suggested that these enzymes differed qualitatively: (a) A plot of M2-PK activity against PEP concentration was sigmoidal similarly to that of L-PK. However, the cooperative interaction of M2-PK with PEP was lower than that of L-PK since the Hill coefficient of M2-PK for PEP was about 1.5. (b) Another similarity between the kinetic properties of M2-PK and L-PK was that both were activated by various organic solvents, such as ethylene glycol and glycerol. The activity curves of both PK's with respect to PEP concentration changed to the usual Michaelis-Menten type in the presence of 25% (v/v) ethylene glycol with disappearance of sensitivity to FDP. In contrast, the activity of M1-PK was not appreciably influenced bv any organic solvent examined.抄録全体を表示PDF形式でダウンロード (1894K) -
Shinichi SEYAMA, Yasuhiro KURODA, Nobuhiko KATUNUMA1972 年 72 巻 4 号 p. 1017-1027
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリー1. Glutamine synthetases [EC 6. 3. 1. 2] from rat and chick livers were purified. They were nearly homogeneous as judged by ultracentrifugation, electrophoresis, and immunological analysis.
2. The two kinds of glutamine synthetase were compared with each other and the results indicated that they have many similar properties. However, they differed slightly in mobility in cellulose acetate membrane electrophoresis, subcellular localization, immunological analysis, and some physical and catalytic properties.
3. On immunodiffusion, the precipitin line produced by chick liver enzyme fused with that of the rat enzyme with spur formation. The two enzymes differed in electrophoretic mobilities and in the salt concentration with which they were eluted from a DEAE-Sephadex A-50 column. Their sedimentation patterns of analytical ultracentrifugation were similar.
4. The purified enzymes catalyzed not only the syntheses of glutamine and glutamyl hydroxamate, but also the glutamotransfer reaction. Their substrate and activator specificities, Michaelis constants, and pH optima were similar, but the ratios of their synthetic to transfer activities differed from each other.
5. The enzyme activity was found in the nuclear-residual, mitochondrial, and soluble fractions of both rat and chick livers, but the microsomal fraction had activity only in rat liver. In rat liver the latter fraction showed the highest activity, while in chick liver the activity in the nuclear-residual fraction was the highest. The behaviors of the two enzymes toward or to solubilization procedures differed from each other.
6. The function of glutamine synthetase was discussed from a phylogenic point of view with special reference to the relationship between the evolution of nitrogen excretion in animals and the evolution of the enzyme.抄録全体を表示PDF形式でダウンロード (2220K) -
Teruo IWASAKI, Toshifumi KIYOHARA, Mitsuyoshi YOSHIKAWA1972 年 72 巻 4 号 p. 1029-1035
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーPotato proteinase inhibitors II-a and II-b were characterized in terms of their major chemical and physicochemical properties. The molecular weights were determined to be approximately 10, 500 for both inhibitors. The inhibitors possess one polypeptide chain as judged from terminal amino acid analyses (1mole of amino-terminal alanine and 1mole of carboxyl-terminal alanine in both inhibitors). Further, the amino acid compositions of the inhibitors were found to be closely related each other. Noteworthy are the comparatively high contents of cystine and glycine, and the lack of methionine and tryptophan in both inhibitors. The isoelectric points were found to be at approximately pH8.5 for inhibitor II-a and pH9.1 for inhibitor II-b, respectively. Both inhibitors were completely inactivated by the modification of free amino groups in the molecules with trinitrobenzenesulfonic acid.抄録全体を表示PDF形式でダウンロード (511K) -
Christine T. ENCE, Marvin A. SMITH1972 年 72 巻 4 号 p. 1037-1039
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (215K) -
Katsutada TAKAHASHI, Sôzaburo ONO1972 年 72 巻 4 号 p. 1041-1043
発行日: 1972/10/25
公開日: 2008/11/18
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Comparative Study on Enzymatic Properties of Acid Carboxypeptidase of Molds of the Genus AspergillusEiji ICHISHIMA, Shigenori SONOKI, Keiko HIRAI, Yoshiaki TORII1972 年 72 巻 4 号 p. 1045-1048
発行日: 1972/10/25
公開日: 2008/11/18
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Takashi SATO, Tatsuzo FUJII, Takao MATSUURA, Kiyoshi UEDA1972 年 72 巻 4 号 p. 1049-1051
発行日: 1972/10/25
公開日: 2008/11/18
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Kiroku SUZUKI, Jun-ichi OOBA1972 年 72 巻 4 号 p. 1053-1055
発行日: 1972/10/25
公開日: 2008/11/18
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Hisayuki MATSUO, Masato OHE, Fumio SAKIYAMA, Kozo NARITA1972 年 72 巻 4 号 p. 1057-1060
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (291K) -
Toshiko NAKAO, Makoto NAKAO, Fumiko NAGAI, Koichi KAWAI, Yoko FUJIHIRA ...1972 年 72 巻 4 号 p. 1061-1063
発行日: 1972/10/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (205K)
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