巻号一覧
72 巻, 5 号
選択された号の論文の31件中1～31を表示しています
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Masahiko KISUMI, Saburo KOMATSUBARA, Ichiro CHIBATA1972 年 72 巻 5 号 p. 1065-1073
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーIn the wild strain of Serratia marcescens D-threonine derepressed isoleucine-valine biosynthetic enzymes (L-threonine dehydratase [L-threonine hydro-lyase, deaminating, EC 4. 2. 1. 16], acetohydroxy acid synthetase and transaminase B [L-leucine: 2-oxo-glutarate aminotransferase, EC 2. 6. 1. 6]). This derepression was overcome by valine or leucine, or both. α-Ketobutyrate inhibited the growth of the wild strain and was antagonized by either valine or leucine, or both. This growth inhibition was accompanied by the derepression of isoleucine-valine enzymes. α-Aminobutyric acid exhibited a similar result. The α-aminobutyric acid-resistant (abu-r) mutants, which were genetically derepressed for the three enzymes and accumulated valine in the medium, had cross-resistance to α-ketobutyrate. The wild strain accumulated isoleucine in the medium containing α-ketobutyrate and α-aminobutyric acid, under the condition where the growth inhibition was partially reversed by the limiting addition of valine plus leucine. The abu-r mutant accumulated a larger amount of isoleucine from these precursors than the wild strain in the presence or absence of valine plus leucine.抄録全体を表示PDF形式でダウンロード (606K) -
Comparison with Skeletal Tropomyosin and TroponinMalcolm P. SPARROW, Francesco M. BOCKXMEER1972 年 72 巻 5 号 p. 1075-1080
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーA procedure for desensitising natural arterial actomyosin by precipitation at I=0.22 is described. The desensitised arterial actomyosin superprecipitated in the absence of Ca2+ and its Mg2+-activated adenosine triphosphatase [EC 3. 6. 1. 3] was increased 7 fold suggesting an inhibitor had been removed. When the soluble protein fraction which had been removed from the natural arterial actomyosin at I=0.22 was replaced, superprecipitation and the Mg2+-activated adenosine triphosphatase activity were inhibited. Skeletal troponin complex was also found to be a potent inhibitor of this desensitised arterial actomyosin, but skeletal and arterial tropomyosin were without effect. Polyacrylamide gel electrophoresis showed that tropomyosin was the chief relaxing protein in the soluble arterial protein fraction, together with a low proportion of protein of a similar electrophoretic mobility to that of skeletal troponin complex. Arterial tropomyosin was functionally and electrophoretically identical to skeletal tropomyosin. It is concluded that a relaxing protein system exists in vascular smooth muscle which functions similarly to that in striated muscle.抄録全体を表示PDF形式でダウンロード (1436K) -
I. Multiple Components and Some Enzymatic Properties of Nicotinamide Adenine Dinucleotide-linked L-Glycerol-3-phosphate Dehydrogenase from Liver and TestesMasahiro YAMADA1972 年 72 巻 5 号 p. 1081-1086
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーMultiple components and some enzymatic properties of L-glycerol-3-phosphate: NAD oxidoreductase [EC 1. 1. 1. 8] from liver and testes in Japanese quail, Coturnix coturnix japonica have been investigated.
Liver enzyme showed five components with isoelectric points at pH7.10 (, designated P-I), 7.38 (P-II), 7.54 (P-III), 7.62 (P-IV), and 7.79 (P-V). P-III among these five components had predominant activity.
Testes enzyme showed four components with isoelectric points at pH4.58, 4.90, 5.26, and 5.46. No predominant component was present among them.
The apparent Km values for L-glycerol-3-phosphate of the partially purified liver and testes enzymes were 3.39 mM and 0.33 mM, respectively, whereas no significant difference in Km value for NAD+ of both enzymes was observed.抄録全体を表示PDF形式でダウンロード (467K) -
Keizo SUZUKI, Yoshihiro TANIGUCHI, Yoshiaki MIYOSAWA1972 年 72 巻 5 号 p. 1087-1091
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーUltraviolet (UV) absorption spectra of aqueous solutions of native and heat-denatured DNA from salmon sperm were measured directly under pressure up to 4, 500 atm at room temperature. There was no detectable change to suggest the pressure denaturation of DNA, whereas the optical density at 260mμ of heat denatured DNA decreased with increasing pressure, and this process was completely reversible. These results can be interpreted to indicate that native DNA is quite stable against pressure and that heat-denatured DNA molecules in random coil are stacked reversibly by the hydrostatic compression. The investigation of visible absorption spectra of native DNA-acridine orange (AO) complex under pressure up to 4, 500 atm also showed the stability of DNA against pressure, but from the spectrum of heat denatured DNA-AO complex, any informations about the conformational change of DNA were not obtained.抄録全体を表示PDF形式でダウンロード (318K) -
Partial Purification and Some Properties of Thiamine Monophosphate KinaseHoyoku NISHINO1972 年 72 巻 5 号 p. 1093-1100
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーThiamine monophosphate kinase, which catalyzes the formation of thiamine pyrophosphate (TPP) from thiamine monophosphate in the presence of ATP and Mg2+, has been purified 60-155 fold from crude extracts of E. coli K12.
The pH optimum of the reaction was around 8.0 and the apparent Km values for thiamine monophosphate and ATP were l.l×lO-6M and 2.7×lO-4M, respectively. Among the univalent cations tested, K+, NH4+, and Rb+ markedly activated the enzyme activity, whereas Li+, Na+, and Cs+ antagonized the stimulatory effect of K+. The enzyme was inhibited by PCMB and the inhibition by PCMB was reversed by addition of 2-mercaptoethanol. The pathway of TPP synthesis is discussed.抄録全体を表示PDF形式でダウンロード (499K) -
Kyozo OGURA, Toshihiro SHINKA, Shuichi SETO1972 年 72 巻 5 号 p. 1101-1108
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーSeparation of geranylgeranyl pyrophosphate synthetase and farnesyl pyrophosphate synthetase [EC 2. 5. 1. 1] was achieved by DEAE-Sephadex chromatography of pumpkin fruit extracts. The purified preparation of geranylgeranyl pyrophosphate synthetase was able to catalyze not only the condensation of isopentenyl pyrophosphate with farnesyl pyrophosphate but also the condensation of isopentenyl pyrophosphate with dimethylallyl or geranyl pyrophosphate, so that it catalyzed the consecutive chain elongation of C5→C10→C15→C20 without accumulation of any intermediate. Mn2+ was an absolute requirement, and Mg2+ was much less effective.抄録全体を表示PDF形式でダウンロード (551K) -
Seiki KURAMITSU, Kiyoshi IKEDA, Kozo HAMAGUCHI, Shiro MIWA, Toshihiro ...1972 年 72 巻 5 号 p. 1109-1115
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーThe interactions of N-acetylglucosamine (NAG), its α- and β-methyl glycosides, di- and tri-NAG with human and hen egg-white lysozymes [EC 3. 2. 1. 17] were studied by the circular dichroic (CD) technique. The association constants obtained for hen lysozyme were in good agreement with the results of other workers. The association constants of the saccharides to human lysozyme were smaller than those for hen lysozyme. The binding free energy of sugar residue at each subsite of the substratebinding site of human lysozyme was compared with that for hen lysozyme and was discussed on the basis of the recent results obtained by the X-ray crystallographic studies.抄録全体を表示PDF形式でダウンロード (469K) -
III. Determination of the StructureSumihiro HASE, Yoshio MATSUSHIMA1972 年 72 巻 5 号 p. 1117-1128
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーAn acidic polysaccharide was isolated from the cell walls of Micrococcus lysodeikticus by lysozyme [EC 3. 2. 1. 17] and L-11 enzyme digestion and successive ECTEOLA-cellulose chromatography. The carboxyl groups of N-acetyl-D-mannosaminuronic acid residues of the acidic polysaccharide was reduced with diborane, and polysaccharide containing equimolar amounts of N-acetyl-D-mannosamine and D-glucose was obtained. A disaccharide mannosaminylglucose was obtained as the major product by the controled acid hydrolysis of the reduced polysaccharide. By nitrous deamination of mannosamine residue in the disaccharide, glucosylglucose was obtained. This disaccharide was hydrolyzed by β-D-glucosidase [EC 3. 2. 1. 21] and it was identified as gentiobiose by gas-liquid chromatography. The disaccharide reduced with borohydride was identified as gentiobiitol by gas-liquid chromatography. The results indicated that the O-β-N-acetyl-D-mannosaminyluronic acid-(1→6)-glucose unit was present in the acidic polysaccharide. The reduced polysaccharide was deacetylated by hydrazine and deaminated with nitrous acid. The polysaccharide thus obtained was subjected to mild acid hydrolysis and the hydrolysate was analyzed by gas-liquid chromatography. The fractions corresponding to maltose and gentiobiose were detected, indicating that the acidic polysaccharide also contained α-1, 4-linkages. From these data and previous results, the structure of the acidic polysaccharide was proposed as follows:_??_D-ManNAcUA(py)β-1, 6_??_D-Glc(py)α-1, 4_??_抄録全体を表示PDF形式でダウンロード (1873K) -
Yoshihiro SATO, Motohiro MARUYAMA1972 年 72 巻 5 号 p. 1129-1137
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリー1. Administration of carbon tetrachloride (CCl4, 0.5-2.5ml/kg body weight) to rats, resulted in decrease in the activity of hepatic tryptophan pyrrolase [L-tryptophan: oxygen oxidoreductase, EC 1. 13. 1. 12] within a few hours. The induction of this enzyme by simultaneous administration of triamcinolone acetonide and L-tryptophan was also markedly suppressed from the first hour after CCl4 treatment.
2. No activator or inhibitor effect was found to participate in this decrease in activity and inducibility of the enzyme. CCl4, at the concentration used did not cause a change in the Km value of pyrrolase or significant release of the enzyme into the blood stream.
3. Measurement of the decrease in enzyme activity after administration of puromycin to CCl4-treated and normal rats, showed that the rates of degradation of pyrrolase in normal and CCl4 treated liver were the same.
4. Actinomycin-mediated superinduction of pyrrolase was inhibited by concomitant administration of CCl4.
5. Pretreatment of rats with aminoacetonitrile reduced the decrease in activity and inducibility of pyrrolase caused by CCl4 while promethazine had no affect.
6. These findings strongly suggest that CCl4 causes decrease in hepatic tryptophan pyrrolase activity and inducibility by disturbance of enzyme synthesis at the translational, rather than the transcriptional step.抄録全体を表示PDF形式でダウンロード (672K) -
Satoshi TAKENAKA, Homare KUWANA1972 年 72 巻 5 号 p. 1139-1145
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーAcetohydroxy acid synthetase of Neurospora crassa was found to be inhibited by L-valine and to a lesser degree by L-isoleucine, when mitochondrial pellet suspension was used for the assay of this enzyme. When acetohydroxy acid synthetase was extracted from mitochondria by various chemical and physical means, its sensitivity to valine was greatly reduced. The inhibition of the enzyme activity by valine was reversed by ATP or ADP. The enzyme was also activated by ATP, ADP, and inorganic orthophosphate. The supernatant prepared by sonic disruption and centrifugation at 215, 000×g contained acetohydroxy acid synthetase which had a broad pH optimum between 8 and 9, and was not affected by valine, ATP, and ADP. Based on the above results the regulation of acetohydroxy acid synthetase activity was discussed.抄録全体を表示PDF形式でダウンロード (483K) -
Yutaka NAGAI, Hisae HORI1972 年 72 巻 5 号 p. 1147-1153
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーVertebrate collagenase has been isolated directly from tissues rich in collagen fibers including basement lamella of tadpole back skin and rat dermis and from human rheumatoid synovial membrane by high speed homogenization at O°C followed by extraction with a neutral salt solution. The activity of collagenase directly extracted from tadpole back skin accounted for about 10-20% of that obtained from the tissue culture. A significant amount of synovial collagenase was present in the membrane in a form of an enzyme-tissue component complex dissociable on treatment with the neutral salt solution at 4°C for 16hr. These findings strongly suggest that vertebrate collagenase produced by enzyme-forming cells is transported to the tissues rich in collagen fibers and stays there at physiological levels in the controlled degradation of collagen in the tissues.抄録全体を表示PDF形式でダウンロード (2312K) -
Yoshiko NAKAE, Kiyoshi IKEDA, Takachika AZUMA, Kozo HAMAGUCHI1972 年 72 巻 5 号 p. 1155-1162
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーTwo of the three tyrosyl and about four of the seven amino groups in the hen egg-white lysozyme [EC 3. 2. 1. 17] molecule were found to be reactive with N-acetylimidazole (NAI). Acetylation of tyrosyl and amino groups resulted in a change in the aromatic circular dichroic (CD) spectrum. On deacetylation of the O-acetyltyrosyl residues, the CD spectrum returned to the native spectrum but not completely. It was suggested that the state of tryptophyl residue(s) was changed by acetylation of tyrosyl and amino groups. The pH-dependence between pH 4 and 12 of the ellipticity at the negative CD band at 305mμ of the unmodified lysozyme was examined in detail. The pH-dependence was resolved into two components: One represents the interaction of Trp-108 and Glu-35 with an intrinsic pK value of 6.9 and the other, the ionization of a tyrosyl residue with an intrinsic pK value of 10.18. Deacetylated lysozyme showed a pH-dependence of the ellipticity at 305 mμ similar to that found for the unmodified protein with a pH shift of about 0.5 to higher pH's on the alkaline side.抄録全体を表示PDF形式でダウンロード (615K) -
Takashi SHIMAKATA, Katsuyoshi MIHARA, Ryo SATO1972 年 72 巻 5 号 p. 1163-1174
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーThe cyanide-sensitive factor (CSF), the terminal oxidase of the stearoyl-CoA desaturase system, was solubilized with detergents from rat liver microsomes and separated by DEAE-Sephadex chromatography from NADH-cytochrome b5 reductase [EC 1. 6. 2. 2] (fp1) and cytochrome b5. An NADH-dependent stearoyl-CoA desaturase activity could be reconstituted by mixing the CSF fraction with detergent-solubilized fp1 (d-fp1) and detergent-solubilized cytochrome b5 (d-b5), both purified from rabbit liver microsomes. The reconstituted activity was similar to that of liver microsomes in pH optimum and behavior towards inhibitors. Omission of any one of the three components led to almost complete loss of the reconstituted activity. Trypsin-solubilized cytochrome b5 (t-b5) was only negligibly active in the reconstitution. When combined with the CSF fraction, lysosome-solubilized fp1 (1-fp1), but not d-fp1, catalyzed the desaturation even in the absence of cytochrome b5. This suggested that 1-fp1, which has been shown to be a proteolytically modified product of d-fp1, had acquired an artificial activity to react directly with CSF. The isolated CSF fraction was colorless, containing no heme and cytochromes, but was rich in phospholipids. It is concluded that cytochrome b5, in addition to fp1 and CSF, is obligatorily involved in the microsomal desaturase system and that CSF is probably not a hemoprotein.抄録全体を表示PDF形式でダウンロード (975K) -
V. Subsites C and D in the Active SiteToshihiko MONODANE, Saburo HARA, Yoshio MATSUSHIMA1972 年 72 巻 5 号 p. 1175-1183
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリー1. The steric relations between the active site of hen egg white lysozyme [EC 3. 2. 1. 17] and bound substrate were investigated. Subsites C and D of the active site were examined using as substrate a hot hydrazine extract of Micrococcus lysodeikticus cells which had been N-acetylated and partially O-methylated.
2. It was found that the 6-O-methyl-N-acetyl-glucosamine residue could fit in subsite C easily, while a 3-O-methyl-, or 3, 6-di-O-methyl-N-acetyl-glucosamine residue could not. The 6-O-methyl-N-acetyl-muramic acid residue did not readily fit in subsite D.
3. 6-O-Methyl-muramic acid used as a reference compound, was synthesized through the methyl N-acetyl-muramide 4-internal ester.抄録全体を表示PDF形式でダウンロード (633K) -
Shuichi HASHIMOTO, Shosuke TAKEMURA, Sadato YABUKI, Katsutoshi KONISHI ...1972 年 72 巻 5 号 p. 1185-1195
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーTyrosine tRNA from T. utilis was split into two half molecules (3'- and 5'-half) by limited RNase T1 [EC 2. 7. 7. 26] digestion, which were separated by DEAE-cellulose column chromatography. No tyrosine acceptor activity was found for the individual half molecule, whereas the recombination of the two halves resulted in a partial recovery of the activity. Sedimentation analyses revealed that the two halves showed the similar hydrodynamic behavior, meanwhile the reconstituted molecule upon the recombination of the two halves exhibited almost the same sedimentation coefficient as the intact molecule The measurements of melting curves of these molecules and their detailed analyses for differential curves suggested that the reconstitution by two half molecules caused a considerable recovery of the conformation toward the intact molecule in respect to hydrogen bondings. Optical rotatory dispersion and circular dichroism indicated that the two halves may have less orderly conformation than the intact molecule, while the reconstituted molecule showed a partial recovery of the intact conformation which was in more ordered structure than those of the two half molecules.抄録全体を表示PDF形式でダウンロード (781K) -
III. Transglycosylation by the Enzyme and Preparation of Crystalline Phenyl N, N'-Diacetyl-β-chitobiosideTomohiro MEGA, Tokuji IKENAKA, Yoshio MATSUSHIMA1972 年 72 巻 5 号 p. 1197-1203
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーN-Acetyl-β-D-glucosaminidase [EC 3. 2. 1. 30] of Aspergillus oryzae was shown to have strong transglycosylation activity, and di- and trisaccharides were produced by the transfer reaction of this enzyme using a high substrate concentration. Using phenyl N-acetyl-β-D-glucosaminide as substrate, two disaccharides were obtained and their structures were determined by permethylation, periodate oxidation, and digestion with enzymes. The results showed that this enzyme transferred the N-acetylglucosamine residue from the substrate to the hydroxyl group at carbon 4 or 6 of the substrate, the respective products being formed in a ratio of about 13:1.
When phenyl N-acetyl-β-D-galactosaminide was used as substrate, the transfer reaction hardly occurred.
3-0-Methylation or 6-O-methylation of the substrate, phenyl N-acetyl-β-D-glucosaminide, had no effect on the transfer reaction using methanol as acceptor. The reaction was only slightly influenced by the pH of the reaction medium.抄録全体を表示PDF形式でダウンロード (481K) -
Masanobu KOMIYA, Shigeharu NAGASAWA, Tomoji SUZUKI1972 年 72 巻 5 号 p. 1205-1218
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーA substance which activated prekallikrein on addition of ellagic acid was isolated from the euglobulin fraction of bovine plasma by chromatographies on CM-Sephadex C-50, DEAE-Sephadex A-50 and once more on CM-Sephadex C-50. This prekallikrein activator existed in plasma in an inactive precursor form and tended to change progressively into an active form during the purification procedure. The final preparation showed about 70% of the full prekallikrein activating activity induced by the addition of ellagic acid. About 1mg of a purified preparation was obtained from 15liters of bovine plasma.
The apparent molecular weight of the activator and its precursor protein was calculated to be 95, 000 by gel filtration and about 89, 000 by sucrose density gradient ultracentrifugation.
The prekallikrein activator had functional activity as Hageman factor and corrected the coagulation defect of Hageman factor-deficient human plasma. Its clot promoting activity was found to be parallel with its prekallikrein activating activity on polyacrylamide gel disc electrophoresis. The prekallikrein activator was similar to the bovine Hageman factor purified by Schoenmakers et al. with respect to its molecular weight, chromatographic behaviours, inhibition by trypsin inhibitor of lima bean, and abilities to activate prekallikrein and to hydrolyze arginine esters.抄録全体を表示PDF形式でダウンロード (1866K) -
Kimio UMEKI, Takehiko YAMAMOTO1972 年 72 巻 5 号 p. 1219-1226
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーThe branched dextrins produced from waxy rice starch β-limit dextrin by Bacillus subtilis' saccharifying α-amylse [EC 3. 2. 1. 1] were isolated by the multiple development paper chromatography and their chemical structures were investigated. The analyses using β-amylase [EC 3. 2. 1. 2], glucoamylase [EC 3. 2. 1. 3], pullulanase, and pullulan α-1, 4-glucoside hydrolase revealed that they were doubly branched dextrins with the following structures: 63-α-(62-α-glucosylmaltosyl)-maltotriose, 63-α-, 65-α-diglucosylmaltopentaose, and 63-α-(63-α-glucosylmaltotriosyl)-maltotriose. The results were discussed in connection with the interior structure of β-limit dextrin.抄録全体を表示PDF形式でダウンロード (3169K) -
VI. The Difference Absorption Spectra Induced by ATP Analogs and Chemical Modification of Tryptophanyl Residue with 2-Hydroxy-5-nitrobenzyl BromideHidenori YOSHINO, Fumi MORITA, Koichi YAGI1972 年 72 巻 5 号 p. 1227-1235
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリー1. Difference spectrum of UV absorption of heavy meromyosin was induced by various nucleoside triphosphates, CTP, TTP, UTP, GTP, and ITP, and by ribose triphosphate. Measurements were made by using a flow apparatus connected to a spectrophotometer or a recording spectrophotometer depending upon the life time of the difference spectrum.
2. The initial velocity of formation of the difference spectrum induced by CTP followed a second order kinetics. The ks value calculated from decay of the difference spectrum agreed with Vm/e of CTPase.
3. The maximum Δε value of the difference spectrum, Δεmμ, was in the order of CTP>TTP>ATP>UTP>ITP>GTP. The ratio, Δε293mμ/Δεmμ indicating the contribution of a tryptophanyl absorption to the difference spectrum was higher in the difference spectrum induced by any nucleoside triphosphate than in that induced by the corresponding diphosphate. The ratio of the difference spectrum induced by ribose triphosphate and tripolyphosphate was very small and similar to that induced by PPi.
4. Two moles of 2-hydroxy-5-nitrobenzyl group were bound per mole of heavy meromyosin by the modification reaction performed in the presence of 0.6M KCl at pH 5.8. The difference spectrum of heavy meromyosin induced by ATP did not change until 1 mole of 2-hydroxy-5-nitrobenzyl group is bound, but decreased to about one half with increase in its binding from 1 to 2 moles per mole of heavy meromyosin.
5. A charge transfer interaction between the base of the nucleoside triphosphate and a tryptophanyl residue is assumed to occur at the active site of heavy meromyosin.抄録全体を表示PDF形式でダウンロード (710K) -
Morimitsu NISHIKIMI, Masataka YOSHINO1972 年 72 巻 5 号 p. 1237-1244
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーThe binding of bromphenol blue (BPB)* to bovine serum albumin (BSA), human serum albumin, or ovalbumin at pH 7.0 caused an enhancement of fluorescence of the dye. The order of enhanced fluorescence intensity of sulfonphthalein dyes bound to BSA was: BPB, brom-chlorphenol blue and bromcresol purple>chlorphenol red>cresol red and bromphenol red>phenol red, bromcresol green, and bromthymol blue. The latter three exhibited little or no enhancement of fluorescence. The fluorescence enhancement is considered to be associated with a planar conformation of dyes induced by their binding to proteins.
The intensity of fluorescence of BSA-bound BPB was found to vary with pH. It increased in both acid and alkaline pH ranges (3.2 to 4.5 and 7.0 to 9.0), where conformational changes of the BSA molecule are known to occur. The modification of absorption spectrum corresponding to the fluorescence change was also observed. Below pH 3.2, the intensity of fluorescence decreased accompanied by a spectral change of BPB, which indicated the conversion of the dye from the basic form to the acidic one.抄録全体を表示PDF形式でダウンロード (551K) -
I. Solubilization, Purification, and Intramicrosomal LocalizationTeruaki AKAO, Tsuneo OMURA1972 年 72 巻 5 号 p. 1245-1256
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーAn acetanilide-hydrolyzing esterase of rat liver microsomes was found to be easily solubilized from the membranes by treatment with phospholipase A [EC 3. 1. 1. 4] (pH 6.8), aqueous acetone, low concentrations of detergents, alkali, or sonic oscillation. Most microsomal enzymes, such as cytochrome b5 and NADPH-cytochrome c reductase, were not solubilized by these treatments. On the other hand, digestion with trypsin [EC 3. 4. 4. 4] did not liberate the esterase from the membranes, although it solubilized cytochrome b5 and NADPH-cytochrome c reductase very effectively.
The esterase was extracted from an acetone powder of trypsin-treated rat liver microsomes and purified about 50-fold by Sephadex G-150 gel filtration and DEAE-Sephadex chromatography. Rabbit antiserum was prepared against the purified esterase. The antibody thus prepared did not inhibit the acetanilide-hydrolyzing activity of the purified enzyme, but precipitated this activity efficiently. However, evidence was obtained that the antibody did not react with the esterase in untreated microsomal vesicles. This suggests that the enzyme in microsomes is not located on the outer surface of the vesicular membranes.
These results are discussed in relation to the binding of the esterase to the endoplasmic reticulum.抄録全体を表示PDF形式でダウンロード (1752K) -
II. Turnover StudiesTeruaki AKAO, Tsuneo OMURA1972 年 72 巻 5 号 p. 1257-1259
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (268K) -
Kiyomichi HANABUSA, Hisayo KOHNO, Shigeo MATSUYAMA1972 年 72 巻 5 号 p. 1261-1263
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (187K) -
Kuniji TANAKA, Chikashi NISHI, Makiko TAKAYA, Takao UCHIYAMA1972 年 72 巻 5 号 p. 1265-1267
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (200K) -
Daisaburo FUJIMOTO1972 年 72 巻 5 号 p. 1269-1271
発行日: 1972/11/25
公開日: 2008/11/18
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Takaharu TANAKA, Takeharu KANEHISA1972 年 72 巻 5 号 p. 1273-1275
発行日: 1972/11/25
公開日: 2008/11/18
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Akiharu WATANABE, Kazuhisa TAKETA1972 年 72 巻 5 号 p. 1277-1280
発行日: 1972/11/25
公開日: 2008/11/18
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Yutaro MOTOKAWA, Goro KIKUCHI1972 年 72 巻 5 号 p. 1281-1284
発行日: 1972/11/25
公開日: 2008/11/18
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Koichi HIRAGA, Hideo KOCHI, Yutaro MOTOKAWA, Goro KIKUCHI1972 年 72 巻 5 号 p. 1285-1289
発行日: 1972/11/25
公開日: 2008/11/18
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Shunji NATORI1972 年 72 巻 5 号 p. 1291-1294
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (198K) -
Noshi MINAMIURA, Kimio UMEKI, Kazuaki TSUJINO, Takehiko YAMAMOTO1972 年 72 巻 5 号 p. 1295-1298
発行日: 1972/11/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (2009K)
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