巻号一覧
82 巻, 1 号
選択された号の論文の42件中1～42を表示しています
- |<
- <
- 1
- >
- >|
-
Noriko MORI, Tohoru NAKAMURA, Jiro KOYAMA1977 年 82 巻 1 号 p. 1-8
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーAdministration of an excessive dose of Bacillus subtilis α-amylase [EC 3. 2. 1. 1, α-1, 4-glucan 4-glucanohydrolase] (BαA) induced the production of non-precipitating (non-ppt) IgG2 antibody in guinea pigs, whereas immunization with a normal dose produced precipitating (ppt) IgGI and IgG2 antibodies. The non-ppt IgG2 antibody thus produced could be isolated from the coexisting ppt IgG2 antibody by means of the precipitin reaction at maximum precipitation. The non-ppt antibody was incapable of forming a precipitin arc with BαA in a conventional agar plate. In the presence of 4% polyethylene glycol (PEG), however, it formed a single arc which fused completely with those of the ppt IgG1 and IgG2 antibodies. The non-ppt antibody could not fix complement, but inhibited BαA activity, though with less efficiency than the ppt antibodies.
These properties of the non-ppt IgG2 antibody may be due to a low affinity for BαA, since both gel filtration and precipitation of soluble antigen-antibody complexes with 20% PEG showed that the antibody was easily dissociable from BαA.抄録全体を表示PDF形式でダウンロード (1194K) -
Tohru OHYAMA1977 年 82 巻 1 号 p. 9-15
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーPhthalate esters have shown to stimulate the latent ATPase [EC 3. 6. 1. 3] activity and induce mitochondrial swelling. Among the tested phthalate esters, di-n-butyl phthalate (DBP) exhibited the greatest activity, and the activity decreased progressively as the alkyl chain was lengthened or shortened. The DBP-stimulated ATPase was oligomycin-sensitive.
The degree of stimulation of the ATPase was proportional to the extent of mitochondrial swelling induced by DBP in 0.1M Tris-HCl (pH 7.2) containing 0.25M sucrose. However, the swelling was dependent on the tonicity of the solution or the concentration of chloride ions, while the stimulation of ATPase was independent of these factors.
Swelling was strongly induced by DBP at slightly acidic rather than neutral or alkaline pH. The pH-activity curve of DBP-stimulated ATPase was in inverse correlation with that of swelling, which showed a rather flat maximum at pH 8.0.
When bovine serum albumin (BSA) was added to a solution containing mitochondria before addition of DBP, swelling was no longer caused by DBP, though the latent ATPase was stimulated to the same extent in the absence of added BSA.抄録全体を表示PDF形式でダウンロード (1093K) -
Yoh OKAMOTO, Koichi YAGI1977 年 82 巻 1 号 p. 17-23
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーMyosin was incubated with a large excess of exogenous g1, g2 or g3 in 0.6M KSCN (or in 4M LiCl) for 1-2 h at 0-2°C. KSCN (or LiCl) was then removed by dialysis. The composition of g-chains in the resulting myosin was analyzed by SDS-gel electrophoresis. When myosin was incubated with g1, the amount of g1 in myosin increased and the increment was nearly counterbalanced by a decrease in g3, whereas an opposite change was observed on incubation with g3. The amount of g2 was not changed by these treatments.
The same ATPase activity as that of control myosin was observed in the presence of Ca2+ or EDTA with the myosins incubated with g1, g2, or g3, but the activity in the presence of Mg2+ was about one-half of the control. The Ca2+ sensitivity of actomyosin containing the treated myosins was slightly higher than that of actomyosin containing the control myosin.
Spin-labeled g1 or spin-labeled g3 was incorporated into myosin, but the ESR spectra of two spin labels were not distinguishable. No information could be obtained from the ESR spectra by the addition of Ca2+, Mg2+, nucleotides or actin.
Inhibition of ATPase activity was observed when SH groups of g1 or g3 in myosin were chemically modified.抄録全体を表示PDF形式でダウンロード (462K) -
Hideto KUWAYAMA, Koichi YAGI1977 年 82 巻 1 号 p. 25-33
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーCardiac myosin was found to contain two small polypeptide components (g1 and g2) as well as the main polypeptide chain (f-chain). A mixture of g1 and g2 was separated from the main chain in 4M urea or 5M guanidine-HCl and then these two proteins were separated by pCMB-Sepharose 4B conjugate column chromatography.
Cardiac myosin subfragment-1 contained g1 as the only small component. Small amounts of unidentified polypeptide fragments were detected in this subfragment-1 preparation by acrylamide gel electrophoresis in the presence of SDS. These polypeptide fragments were removed by gel filtration using Sephadex G-200 in the presence of 4M urea.
The UV absorption spectra and electrophoretic mobilities of small polypeptide components of myosin and subfragment-1 were compared after isolation. It was concluded that i) g1 of subfragment-1 was identical with that of myosin, ii) g2 has an absorption spectrum characteristic of Ca2+ binding proteins, and iii) subfragment-1 was readily degraded by chymotrypsin into another form of subfragment-1 in which the small component was smaller than g1.
Ca2+ binding to g2 was demonstrated using murexide as a color indicator. The UV difference spectrum of g2 was observed with and without Ca2+. The dissociation constant was estimated to be 3.1×10-4M. No evidence for Ca2+ binding to g1 was obtained.抄録全体を表示PDF形式でダウンロード (2348K) -
Keiko KOGA, Yoko KANAZAWA, Katsuhisa TAWADA1977 年 82 巻 1 号 p. 35-41
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe Mn (II)-heavy meromyosin system was studied by measuring the ESR spectrum of Mn(II). The temperature dependence of the line width parameter W(1, t) of a freshly prepared sample changes at around 7-10°C, where W(1, t) is the reciprocal of the peak-to-peak height of the lowest magnetic field component of the hyperfine structure. It is shown that the change in the slope of W(1, t) at 7-10°C is due to a change in the structure of Mn (II)-heavy meromyosin or a change in the interaction between Mn(II) and heavy meromyosin without ATP. This result is in accord with the recently reported observations that heavy meromyosin ATPase activity showed different temperature dependences above and below 10°C in the presence of Mn (II).
The characteristics of the spectrum of the Mn (II)-heavy meromyosin system in the liquid state between 2°C and 20°C are compared with those of a frozen sample of Mn (II)-heavy meromyosin in a low temperature region (-50-0°C) and with those of the lyophilized material. The forbidden transitions are observed, and hence the zero field splitting parameter can be obtained. It is 115±15 gauss at -50°C, and decreases with increase of the temperature to 70±15 gauss at 20°C.抄録全体を表示PDF形式でダウンロード (513K) -
Yukio KATO, Takako KATO, Hisataka KASAI, Tsuneo OKUYAMA, Keiichi UYEMU ...1977 年 82 巻 1 号 p. 43-51
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーTwo highly acidic protein preparations (chick brain acidic protein: CBA I and II) were obtained from chick brain by extraction with 55% saturated ammonium sulfate, followed by DEAE-Sephadex A-50 column chromatography and Sephadex G-75 gel chromatography. Both proteins migrated with bromphenol blue (BPB) marker dye on 7.5% polyacrylamide gel disc electrophoresis. CBA I gave essentially a single band on 7.5% polyacrylamide gel electrophoresis, but showed slight contamination on 15% gel. On the other hand, CBA II was homogeneous on both 7.5% and 15% polyacrylamide gel disc electrophoresis. The yields of CBA I and CBA II were about 40mg and 700mg, respectively, from 9kg of chick brain. CBA II showed a characteristic absorption spectrum in the region from 250 to 280nm, presumably due to phenylalanine residues in the protein. Molecular weights were estimated to be 14, 500 for CBA I and 16, 000 for CBA II by SDS-polyacrylamide gel electrophoresis. One major N-terminal amino acid residue in addition to two minor ones was detected for CBA I by the dansylation method, but no N-terminal residue could be detected in CBA II. Amino acid compositions indicated that the two protein preparations were rich in acidic and hydrophobic amino acid residues. The peptide maps of tryptic peptides were different. CBA I cross-reacted with the antiserum against bovine S-100 protein, but CBA II did not. Based on these results, it was concluded that CBA I contained S-100 protein and was different from CBA II. In addition, both proteins were compared with two highly acidic proteins purified from bovine brain (PAP I and PAP II). CBA I was different from PAP I, but CBA II was virtually identical with PAP II in amino acid composition.抄録全体を表示PDF形式でダウンロード (2181K) -
III. Isolation and Characteristics of Some Calamus ProteinsSoichi MUROZONO, Kunio MURAYAMA, Kiso AKAHANE1977 年 82 巻 1 号 p. 53-58
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe main fraction, GF-3, separated from SCM-proteins of fowl feather calamus by gel filtration, was separated into seven peaks, C-1 to C-7, by ion exchange chromatography on a DEAE-cellulose column. Each fraction was found to contain two components by polyacrylamide gel electrophoresis.
In order to separate these two components, recycling gel filtration on a Sephadex G-75 column was carried out. Two peaks separated from each of four fractions, C-3 to C-6, were termed C-3a and -3b, C-4a and -4b, and so on. Electrophoretic results showed that all the fractions were single protein components. Components a and b of a particular fraction had almost identical electric charge and slightly different molecular size.
All components in Group a (C-3a to C-6a) had similar amino acid compositions except for SCM-cysteine content, and similar results were obtained for Group b (C-3b to C-7b). Groups a and b had significantly different amino acid compositions.抄録全体を表示PDF形式でダウンロード (2385K) -
Fusayuki KANDA1977 年 82 巻 1 号 p. 59-66
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe nuclear ribonucleoprotein (RNP) particles containing rapidly labeled RNA were isolated from interphase cells of the cellular slime mold Dictyostelium discoideum and characterized. The size of the isolated RNP particles was small (10S to 50S) in comparison with that of nuclear RNP particles found in higher eukaryotes. These small RNP particles do not seem to be artifacts due to degradation during the preparation of nuclear extracts. The rapidly labeled RNA of the nuclear RNP particles was heterogeneous in size and a considerable amount contained polyadenylic acid sequences. Synthesis of RNA in the nuclear RNP particles was resistant to a relatively high concentration of actinomycin D. The protein component of the RNP particle consists of at least four proteins with molecular weights of 80, 000, 66, 000, 60, 000, and 42, 000. Thus it is suggested that almost all of the nuclear RNP particles containing rapidly labeled RNA in interphase cells are RNP complexes consisting of heterogeneous nuclear RNA and several protein species.抄録全体を表示PDF形式でダウンロード (565K) -
Takashi UEDA, Kiyoshi GODA, Terutoshi MORI, Edahiko MURAKAMI, Yahito K ...1977 年 82 巻 1 号 p. 67-72
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーAn equimolar mixture of a xanthurenic acid-insulin complex and ZnSO4 was separated into an insulin peak and a peak containing xanthurenic acid (XA) and Zn by Sephadex G-75 column chromatography. XA and di-[L-histidino]-zinc (II) readily combined to produce di-[L-histidino]-di-xanthurenato zinc (II) (His2-Zn2+-XA2). By increase in the concentration of Zn2+ ions, XA was removed from di-[L-histidino]-di-xanthurenato zinc (II) (His2-Zn2+-XA2) as XA-Zn2+. The XA-insulin complex showed decreased relative intensity of fluorescence compared with the Zn-insulin when excited at a wavelength of 284nm. The difference spectrum between native Zn-insulin and XA-insulin complexes showed a slight red shift. A difference in the CD spectrum between native Zn-insulin and XA-insulin complexes was observed.抄録全体を表示PDF形式でダウンロード (339K) -
Nanaya TAMAKI, Masayuki NAKAMURA, Keiko KIMURA, Takao HAMA1977 年 82 巻 1 号 p. 73-79
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーA procedure for the purification of aldehyde dehydrogenase from bakers' yeast (Saccharomyces cerevisiae) is reported. Treatment with acid, heat and organic solvents was avoided and chromatographic and filtration techniques in the presence of phenylmethylsulfonylfluoride were mainly used. An affinity chromatography step using the reactive dye Cibacron blue F3G-A, which was covalently bound to Sepharose 4B, was found to be essential. The enzyme was bound to and then released from the dye. The purified enzyme was shown to be homogeneous by gel filtration, disc electrophoresis and SDS electrophoresis. The molecular weight of the purified enzyme determined by gel filtration was 170, 000, which agreed with that of the enzyme in the crude extract. The enzyme was composed of subunits of a molecular weight of 57, 000.
The specific activity of the enzyme was 20 units per mg of protein under the standard assay conditions. The substrate specificity, the relative maximal velocity, the Michaelis constants, the pH optimum, the stability and the activation energy of the enzyme are reported.抄録全体を表示PDF形式でダウンロード (1085K) -
Hiroyuki SUMI, Nobuo IZUMIYA, Mutumi MURAMATU1977 年 82 巻 1 号 p. 81-85
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe change of enzyme activity in immunized rabbit plasma after addition of the homologous antigen was examined.
The activities of Nα-tosyl-L-arginine methyl ester (TAMe) and Nα-tosyl-L-lysine methyl ester (TLMe) hydrolysis increased about 15 to 18 days after immunization. This increase was especially marked before the maximal rise of antibody content, and is thought to be related to the IgM antibody not to the IgG antibody.
Enzyme activation was strongly inhibited by chelation of Ca2+ with 5mM disodium ethylenediamine tetraacetate (EDTA), but not by other protease inhibitors, such as epsilon aminocaproic acid (ε-ACA), bovine lung kallikrein inhibitor (Trasylol) or soybean trypsin inhibitor (SBTI).抄録全体を表示PDF形式でダウンロード (323K) -
I. Separation of Proteins from Cell Envelope of Escherichia coliMasao KOZUKA, Hiroshi HAYASHI1977 年 82 巻 1 号 p. 87-94
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーRadioactive proteins from Escherichia coli cell envelope fraction were separated by twodimensional polyacrylamide gel electrophoresis. Electrophoresis was carried out under several sets of conditions, and autoradiographs were obtained. Many of the proteins were separated at well-defined positions with good reproducibility. Some of the proteins moved relative to these stationary proteins depending at least two factors, i.e. the amount of proteins applied in the first dimension and the electric current applied in the second dimension. Among more than 200 spots, methyl-accepting chemotaxis protein and flagellin were identified by using labelled or cold preparations of these proteins as markers. Some of the spots were assigned to proteins from the outer membrane of the bacteria. The results provide a good foundation for comparative studies of membrane proteins from genetically altered strains of the bacteria.抄録全体を表示PDF形式でダウンロード (2341K) -
Masahiko KISUMI, Saburo KOMATSUBARA, Ichiro CHIBATA1977 年 82 巻 1 号 p. 95-103
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーLeaky revertants isolated from isoleucine auxotrophs of Serratia marcescens mutant resistant to α-aminobutyric acid were previously reported to accumulate leucine in the medium, due to the absence of both feedback inhibition and repression of leucine biosynthesis. Growth of the revertant was accelerated by pyruvate, D(-)-citramalate, citraconate, and α-ketobutyrate, but not by threonine. Extracts of the revertant exhibited high activities of pyruvate-dependent coenzyme A liberation from acetyl-coenzyme A, hydration of citraconate, and conversion of citraconate to α-ketobutyrate, but showed no threonine-deaminating activity. In the leucineaccumulating revertants the above three activities were not affected by leucine, but in the wild strain and other revertants accumulating no leucine all or one of these activities was controlled by leucine. A leucine auxotroph isolated from the leucine-accumulating revertant showed isoleucine auxotrophy as well. From these data, it is concluded that, in leucine-accumulating revertants of S. marcescens, isoleucine is synthesized from α-ketobutyrate via citramalate formed from pyruvate and acetyl-coenzyme A by leucine biosynthetic enzymes, as a result of desensitization of α-isopropylmalate synthetase to feedback inhibition.抄録全体を表示PDF形式でダウンロード (587K) -
Masahiro ISHIURA, Kazuko SHIBATA-SEKIYA, Toyoki KATO, Yuji TONOMURA1977 年 82 巻 1 号 p. 105-115
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーTwo kinds of subfragment-1 of myosin, S-1(T) and S-1(CT), were prepared by two-step tryptic [EC 3. 4. 21. 4] digestion of myosin that had been modified with about 1 mol of p-chloromercuri-benzoate (CMB) per mol of myosin, and one-step chymotryptic [EC 3. 4. 21. 1] digestion of the myosin, respectively. The amount of bound CMB was about 0.82-0.90 mol per 2 mol of S-1.
Both kinds of S-1 modified with CMB equally inhibited superprecipitation of myosin B from rabbit skeletal muscle. About 2 mol of CMB-S-1 (1 mol of CMB-S-IA) inhibited the function of 1 mol of actin monomer on the superprecipitation of actomyosin reconstituted from myosin and fibrous actin(FA) with relaxing protein (RP). CMB-S-1 also effectively inhibited superprecipitation of myosin B from the plasmodia of the slime mold Physarum polycephalum.
The ATPase [EC 3. 6. 1. 3] activity of CMB-S-1(T) was similar to that of CMB-S-l(CT) in the absence of FA, but was not enhanced as effectively by FA as the latter. In the presence of 0.3mg/ml of FA with RP, the activity of CMB-S-1(T) was only one-fifth of that of CMB-S-1 (CT).
CMB-S-1(T) did not affect the activities of ATPase from animal cells outside actomyosin systems, such as the Ca2+-dependent ATPase [EC 3. 6. 1. 3] of the SR prepared from rabbit skeletal muscle and the Na+, K+-dependent ATPase [EC 3. 6. 1. 3] from porcine kidney. It also scarcely affected Ca2+-uptake by the SR at concentrations lower than 0.2mg/ml.
However, CMB-S-1(T) strongly inhibited the polymerization and depolymerization of tubulin prepared from bovine brain. At 0.15 mol per mol of tubulin heterodimer, CMB-S-1(T)抄録全体を表示PDF形式でダウンロード (841K) -
Taneaki HIGASHI, Noriko TATEISHI, Akiko NARUSE, Yukiya SAKAMOTO1977 年 82 巻 1 号 p. 117-124
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリー1) To verify the presence of more than one pool of glutathione in the liver, a diet containing L-[35S]cysteine was given to rats, and after chasing and exhaustion of the 35S-label a second diet containing L-[3, 3-3H]cystine was given. Starvation of the rats thus treated revealed differential decay of 35S and 3H incorporated into liver glutathione: 3H incorporated later into glutathione disappeared rapidly on starvation, while a large portion of 35S incorporated earlier and remaining in the glutathione did not. A concomitant increase in 3H-label of hepatic proteins on starvation indicated that cysteine derived from glutathione was probably incorporated into proteins.
2) Analysis of the decay curve of the 35S-labeled cysteine moiety of liver glutathione under normal feeding conditions revealed at least two types of glutathione with different biological half-lives. The apparent half-lives were 1.7 and 28.5h. Precipitous decay of glutathione-35S in the initial phase of starvation was accompanied by a rise of incorporation of 35S into proteins. 3) To prove mobilization of cysteine moiety of liver glutathione, a gelatin-diet containing 35S-cysteine was first given to rats followed by a tryptophan-fortified diet. Ingestion of the ‘tryptophan’ diet induced decrease of accumulated glutathione and increase of 35S-label in hepatic and serum proteins, especially that in serum albumin.
4) The most plausible explanation for these results is as follows: There are at least two pools of glutathione, differing from each other in their half-lives. The cysteine moiety of the ‘liver glutathione with shorter half-life’is mobilized for protein synthesis when the other conditions are fulfilled and the amount of cysteine becomes rate-limiting. Otherwise, ‘cysteine’ continues to be stored in liver glutathione. Another pool of glutathione must serve for many reactions requiring sulfhydryl compounds in the liver.抄録全体を表示PDF形式でダウンロード (605K) -
Kunio FUJIWARA, Kuniyo INOUYE, Ben'ichiro TONOMURA, Sawao MURAO, Daisu ...1977 年 82 巻 1 号 p. 125-130
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーSubtilisin BPN' was chemically converted to thiolsubtilisin and the interaction of this modified enzyme with Streptomyces subtilisin inhibitor (SSI) was examined. SSI competitively inhibited the esterolytic activity of thiolsubtilisin toward p-nitrophenyl acetate with a K1 value of 1.3×10-5M at pH 7.5. Spectrophotometric analysis of the interaction between SSI and the modified enzyme yielded a Kd value of 4×10-5M at pH 9.7. These values are about 105-fold greater than the Kd value (less than 10-9M at pH 7.5) for the native enzyme. This indicates that the small change in the active site structure of subtilisin (Ser221 to Cys221) leads to a considerable decrease in the binding affinity (by about 6-7kcal/mol) to SSI.抄録全体を表示PDF形式でダウンロード (410K) -
Hitoshi UENO, Sho TAKAHASHI, Tatsuo OOI1977 年 82 巻 1 号 p. 131-138
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーOne cysteinyl residue in a polypeptide chain of rabbit skeletal α-tropomyosin subunit was titrated with PCMB in accord with the reported sequence. When α-tropomyosin was treated with 2-nitro-5-thiocyanobenzoic acid, the specific peptide bond between Lys 189 and Cys 190 was cleaved to yield two fragments with molecular weights of 25, 000 and 11, 000 daltons as determined by SDS-gel electrophoresis; these were designated as the N-chain (residues 1 to 189 in the intact chain) and the C-chain (residues 190 to 284). Each fragment was separated by a gel filtration method in the presence of urea. The apparent molecular weights of the two fragments in the absence of urea, estimated by a gel filtration technique, were about 110, 000 and 40, 000 daltons for the N- and C-chains, respectively, in 1M NaCl at pH 7.0, indicating association of the individual chains after the removal of urea. Gel electrophoretic patterns of both chains after the removal of urea showed a single band for the N-chain and a double band for the C-chain; no complex formation between the N-chain and the C-chain could be observed. The N-chain showed 64% a-helical content and the C-chain 46% in 20mM phosphate buffer at pH 7.0 as determined by CD measurements. These α-helical contents were increased by the addition of salts. An enhancement of the α-helical content was also observed when the chains were mixed together. Troponin had no significant binding capacity with either of the chains, while the mixture of both chains showed a clear binding with troponin in gel electrophoresis, suggesting that some cooperative interaction must exist between the N- and C-chain mixture and troponin. The binding was independent of Ca2+ in solution.抄録全体を表示PDF形式でダウンロード (2268K) -
An Examination of Difference SpectraTakenori YAMADA, Hiroshi SHIMIZU, Mamoru NAKANISHI, Masamichi TSUBOI1977 年 82 巻 1 号 p. 139-144
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe exchange reaction of peptide hydrogens with deuterium has been followed by measuring the decrease of the amide II band for heavy meromyosin (HMM). The difference spectra between HMM and HMM+ATP, between HMM and HMM+ADP, and between HMM+ ATP and HMM+ADP have been examined as functions of time in order to detect small differences in the kinetic behavior of these different states of HMM. It has been found that, at 14°C and 26°C (pH 8.0), the exchange reaction is slightly slower for HMM+ATP than for HMM, and slightly slower for HMM+ADP than for HMM+ATP. This indicates that the secondary structure of HMM changes its flexibility during the ATP splitting cycle.抄録全体を表示PDF形式でダウンロード (353K) -
Kazuo MOTOYOSHI, Kentaro IWASAKI, Yoshito KAZIRO1977 年 82 巻 1 号 p. 145-155
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーWe have previously shown that eukaryotic polypeptide chain elongation factor 1 (EF-1) from pig liver could be resolved into two complementary factors, EF-1α and EF-1β, of which the former corresponds to one of the bacterial elongation factors, EF-Tu. A brief description of the purification of EF-1β has also been presented (Iwasaki, K., Motoyoshi, K., Nagata, S., &Kaziro, Y. (1976) J. Biol. Chem. 251, 1843). This paper describes the purification procedure of EF-1β in detail as well as some properties of the purified factor, which was renamed EF-1βγ. The purification procedure includes an aqueous two-phase separation of 15, 000×g supernatant of pig liver homogenate, ammonium sulfate fractionation, treatment with sodium cholate and two successive column chromatographies on diethylaminoethyl-Sephadex A-50. By this procedure, EF-1βγ was purified about 40-fold starting from the material obtained after cholate treatment with a recovery of 20%. The purified EF-1βγ appeared to be homogeneous as judged by polyacrylamide gel electrophoresis. It had a molecular weight of about 90, 000, and consisted of two unequal subunits of molecular weights of 55, 000 and 30, 000. The purified EF-1βγ stimulated three reactions: i) polymerization of phenylalanine dependent on poly(U) in the presence of both EF-1α and EF-2, ii) EF-1α-dependent binding of phenylalanyl-tRNA to ribosomes in the presence of GTP but not in the presence of guanyl-5'-yl methylenediphosphonate, and iii) the exchange of GDP bound to EF-1α with exogenous GTP. These results strongly indicate that the function of EF-1βγ is to stimulte the conversion of EF-1α•GDP to EF-1α•GTP, which results in the rapid recycling of EF-1α, and is, at least in part, similar to that of bacterial EF-Ts.抄録全体を表示PDF形式でダウンロード (1507K) -
Sigeru IKUTA, Kazuo MATUURA, Sigeyuki IMAMURA, Hideo MISAKI, Yosifumi ...1977 年 82 巻 1 号 p. 157-163
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーOne strain of bacteria which showed high H2O2-generating activity was isolated from soil and characterized as Arthrobacter globiformis based on its morphological, nutritional, and physiological characteristics. The activities of H2O2 generation, NAD reduction and oxygen consumption in the bacterial cells were examined using choline, betaine aldehyde or betaine as substrate. Choline was oxidized to betaine aldehyde under aerobic conditions in a reaction coupled with H2O2 generation and oxygen consumption. On the other hand, betaine aldehyde seemed to be oxidized to betaine through two distinct oxidative reactions, H2O2 generation (oxygen consumption) under aerobic conditions and NAD reduction under either aerobic or anaerobic conditions. These enzyme activities were found in the supernatant fraction of the sonicated cell preparation.抄録全体を表示PDF形式でダウンロード (498K) -
Takashi SHIMIZU, Ichiro KIMURA1977 年 82 巻 1 号 p. 165-173
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーCiliary 30S dynein of Tetrahymena was investigated with regard to modification of the ATPase activity with N-ethylmaleimide (NEM) in the presence of ATP. The elevation of enzyme activity due to the modification was largely repressed by addition of ATP at a concentration of 1mM or more during preincubation for 20 h at 0°C. The repression was highly specific for ATP, though ADP and AMPPNP showed slight repressive effects. After complete hydrolysis of ATP added to the preincubation mixture, however, elevation of 30S dynein ATPase activity occurred. It is suggested that the repression by ATP of NEM-induced elevation of 30S dynein ATPase activity is simply due to a protecting effect of ATP on certain SH group(s) (probably SH1-type group(s)) around the active center of 30S dynein. When 30S dynein was maximally activated by modification with NEM, ATP or ADP did not significantly promote the inactivation of the modified enzyme upon further treatment with NEM, indicating that 30S dynein lacks the characteristics of SH2-type groups. On the other hand, ATP also showed a protective effect against inhibition of native 30S dynein by high concentrations of NEM.
High concentrations of ADP and AMPPNP were inhibitory to 30S dynein ATPase activity but inorganic phosphate did not inhibit 14S or 30S dynein ATPase activities at all.抄録全体を表示PDF形式でダウンロード (699K) -
Hideo OHYAMA, Ken HASHIMOTO1977 年 82 巻 1 号 p. 175-183
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーCollagenase of human basal cell epithelioma was purified by sequential ammonium sulfate precipitation, Sephadex gel filtration and affinity chromatography on collagen-polyacrylamide gel. The collagenase, when partially purified, was found to have an approximate molecular weight of 50, 000. The purified enzyme contained no caseinolytic activity. On polyacrylamide gel electrophoresis, the purified enzyme gave a single protein band.
The purified collagenase cleaved native acid-soluble guinea pig skin collagen at 37°C with a pH optimum of 8. The enzyme was inhibited by EDTA, cysteine, and human serum but not by soybean trypsin inhibitor. Heparin did not stimulate the enzyme activity. Purified collagenase reduced the specific viscosity of native acid-soluble guinea pig skin collagen to 50 per cent of its original value at 27°C. Polyacrylamide gel disc electrophoresis of the reaction products showed bands corresponding to αA, βA, and αB fragments. Electron microscopic examination of SLS aggregates of the reaction products showed that the cleavage site by the enzyme was at a point 75 per cent from the “A” end (TCA75) and 25 per cent from the “B” end (TCB25) of the collagen molecule.抄録全体を表示PDF形式でダウンロード (1922K) -
Large Hammett's ρ Constant and Participation of Histidine-acylated IntermediateNobuo SHIMAMOTO1977 年 82 巻 1 号 p. 185-193
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーA detailed examination of the mechanism of the hydrolysis of phenyl acetates by α-chymotrypsin [EC 3. 4. 21. 1] was carried out. The effective deacylation rate constants of some phenyl acetates obtained by titration of the acetyl-enzyme decreased at low substrate concentrations and showed anomalous pH dependences and solvent isotope effects. The transient kinetics of deacylation of the acetyl-enzyme were biphasic. A spectrum and a breakdown rate similar to those of acetylimidazole were observed when the acetyl-enzyme was denaturated with sodium dodecyl sulfate. These results indicate the participation of histidine-acylated enzyme, which would account for the anomalous phenomena previously found in this system, including a large value of Hammett's ρ. The relation between the substrate activation and the two intermediates is discussed.抄録全体を表示PDF形式でダウンロード (679K) -
Miyako ABE, Makoto MATSUDA1977 年 82 巻 1 号 p. 195-200
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe in vivo effects of convulsant drugs (hydrazine and penicillamine) on the metabolism of γ-aminobutyric acid (GABA) in subcellular fractions of mouse brain were studied. Both substances inhibited the activity of glutamic acid decarboxylase [EC 4. 1. 1. 15] (GAD) in the synaptosomal fraction (nerve ending particles) and reduced the concentration of GABA in the same fraction at the onset of convulsions, though changes in the total GABA concentration in the brain did not correlate with the onset of convulsions. Therefore, it is suggested that the concomitant decrease of GAD activity and GABA concentration in the nerve endings, independently of the total GABA concentration, is probably an important factor in the onset of some kinds of convulsions.抄録全体を表示PDF形式でダウンロード (394K) -
Sadashi HATANO, Katsushi OWARIBE1977 年 82 巻 1 号 p. 201-205
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーA new, simple method for the isolation of actin from myxomycete plasmodia has been developed. Plasmodium myosin B was incubated at 55°C for 15min in the presence of ATP or was treated with 90% acetone. By this treatment myosin was denatured completely. Actin was then extracted with a dilute ATP and cysteine solution from the heat- or acetone-treated myosin B. The method is simple and almost pure actin was obtained in high yield.
The purified G-actin polymerized to F-actin on addition of 0.1M KCl or 2mM MgCl2. The viscosity of the purified F-actin was 8-10dl/g. The F-actin activated muscle myosin ATPase, and actomyosin synthesized from the F-actin and muscle myosin showed superprecipitation on addition of ATP.抄録全体を表示PDF形式でダウンロード (979K) -
Toshiharu MURAOKA, Hiromichi OKUDA1977 年 82 巻 1 号 p. 207-213
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe relationship between pseudocholine esterase [acylcholine acyl-hydrolase, EC 3. 1. 1. 8] and non-specific esterase [carboxylic ester-hydrolase, EC 3. 1. 1. 1] in human serum was investigated.
The purified preparation (purified 500-fold) which had both pseudocholine esterase and non-specific esterase activities, was found to give a single band with faint tailing on polyacrylamide gel electrophoresis. The ratio of the specific activity of pseudocholine esterase to that of non-specific esterase remained essentially the same during the purification procedures. Furthermore, the pseudocholine esterase was demonstrated to be identical with the non-specific esterase by immunochemical studies. All these results suggest that activities of pseudocholine esterase and non-specific esterase in human serum derive from the same enzyme molecule.
Observation at Yoshida-cho in Ehime after the application of organophosphorus insecticide supported our results: the activity of pseudocholine esterase was found to be reduced with a concomitant decrease in the activity of non-specific esterase. Based on these results, the physiological significance of the esterase is discussed.抄録全体を表示PDF形式でダウンロード (441K) -
Yukio SUZUKI, Takashi MURACHI1977 年 82 巻 1 号 p. 215-220
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe lysate of the glycogen-induced macrophages in rat peritoneal exudate was fractionated by centrifugation and extraction into a water extract, 1M KCl extract and residue fractions. Approximately 50% of the neutral protease activity toward casein in the lysate was recovered in the KCl extract fraction, which was practically devoid of acid protease, cathepsin D. The pH optimum of the neutral protease toward casein and urea-denatured hemoglobin was pH 8.5. The activity was inhibited strongly by DFP or chymostatin and only partially by HgCl2 or PCMB. Addition of a salt to the reaction medium caused enhancement of the activity with an optimum concentration of 0.25M: KCl, KBr, KI, NaCl, NaBr, NaT, and MgCl2 were all almost equally effective. When the enzyme preparation was filtered through a column of Sephadex G-75 gel in the presence of 1M KCl, a larger molecular weight fraction at the void volume was obtained in addition to a smaller molecular weight fraction showing a caseinolytic activity insensitive to KCl concentration. The former was found to have a specific inhibitory effect on the latter activity.抄録全体を表示PDF形式でダウンロード (394K) -
Dorothy A. GIBBS, Sunna HAUSCHILDT, R. W. E. WATTS1977 年 82 巻 1 号 p. 221-230
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe enzyme-catalyzed oxidation of glyoxylate to oxalate by subcellular particles of rat liver and kidney, and in the cytosol fraction (100, 000×g supernatant) of rat kidney, has been investigated. Catalytic activity is associated with liver peroxisomes and rough endoplasmic reticulum, but not with liver lysosomes and mitochondria. The activity associated with the endoplasmic reticulum has the properties of lactate dehydrogenase [EC 1. 1. 1. 27], whereas the peroxisomal activity is attributed to both glycollate oxidase [EC 1. 1. 3. 1.] and to lactate dehydrogenase. Among the particulate fractions of kidney, catalytic activity with respect to the oxidation of glyoxylate to oxalate is due to lactate dehydrogenase associated with the endoplasmic reticulum. The possibility that some of the catalytic activity in kidney tissue is associated with the lysosomes cannot be completely excluded on the basis of the present evidence. No evidence was obtained for peroxisomal or mitochondrial enzymes with catalytic activity with respect to the oxidation of glyoxylate to oxalate in renal tissue. The catalytic activity for the oxidation of glyoxylate to oxalate in the kidney cytosol fraction was NAD+ dependent and associated with lactate dehydrogenase. These results are discussed in relation to previous work and to the problem of excessive oxalate production in man. It would be theoretically desirable to treat the hyperoxaluric diseases by reducing the excessive rate of oxalate biosynthesis in vivo. This could be most effectively done by inhibiting the last step on the oxalate biosynthetic pathway. The present evidence shows that it would be necessary to inhibit lactate dehydrogenase throughout the body as well as glycollate oxidase in order to achieve this.抄録全体を表示PDF形式でダウンロード (702K) -
Shin-ichi SATO, Yuji KARASAKI, Motonori OHNO1977 年 82 巻 1 号 p. 231-237
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe interaction between α-chymotrypsin [EC 3. 4. 21. 1] and peptide substrate or peptide inhibitor was investigated to determine how the secondary interaction influences the rate of hydrolysis or the binding and whether or not its effect is variable with alteration of the P1 residue which interacts with the specificity determining site of the enzyme. Kinetic analysis was carried out at pH 6.5 and 7.8 for substrates of the type Ac-Glyn-X-OMe and for inhibitors of the type Ac-Glyn X-OH where X denotes tryptophan or its derivatives. With substrates containing tryptophan or Nin-formyltryptophan, the second-order rate of hydrolysis increases with increase of chain length. With substrates containing 2-(2-nitro-4-carboxyphenylsulfenyl)tryptophan, however, the rate of hydrolysis decreases with elongation of the chain, due to an increase in Km (app). The corresponding inhibitors behave differently from the other series of inhibitors at pH 6.5. The results indicate that the influence of the secondary interaction on reactivity or binding is related to the structural features of the P1 residue.抄録全体を表示PDF形式でダウンロード (464K) -
Yoshihiro MIYAKE, Naoko TAKAYAMA, Miho FUKUYAMA, Yasuki NONAKA1977 年 82 巻 1 号 p. 239-249
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThree forms of cytochrome P-450 in rat liver microsomes (Comai, K. and Gaylor, J. L. (1973) J. Biol. Chem. 248, 4947-4955) were characterized as two types of P-450 in the membranebound state. One was reactive not only toward cyanide but also aniline, aminopyrine, and hexobarbital, and the other was less reactive toward cyanide and rather unreactive toward the drugs. Cyanide titrations of microsomes in the presence and absence of the drugs were performed spectrophotometrically for analysis of the interactions. The affinity of cyanide for the less reactive P-450 was about twenty times less than that for the reactive P-450. About 40% of P-450 in the microsomal membrane was reactive and the rest was the less reactive form. The reactive P-450 involved two forms having different affinities for cyanide. The ratio of their amounts was 1:2. Triton WR-1339 interfered with cyanide binding to the reactive P-450 mainly. The relative amount of each form of P-450 as well as the dissociation constant of cyanide could be estimated by the present method, and the modes of interaction of the membrane-bound P-450 with the drugs and exogenous ligands were deduced.抄録全体を表示PDF形式でダウンロード (767K) -
Haruo SUZUKI1977 年 82 巻 1 号 p. 251-260
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe effect of concentration of KCl, magnesium acetate (MgAc) and spermine on the translation of rabbit globin mRNAs was studied by using the rabbit reticulocyte lysate system. Globin synthesis was studied by using L-[U-14C]leucine or L-f[35S]Met-tRNAMetf.
The optimum concentrations of MgAc and KCl were 1.4-2.5mM and 70mM, respectively. Spermine stimulated rabbit globin synthesis at relatively low concentrations of MgAc, but inhibited it at relatively high concentrations of MgAc. High α/β synthetic ratios were observed at low concentrations of KCl and MgAc, but low α/β synthetic ratios were observed at high concentrations of KCl and MgAc. Spermine decreased the α/β synthetic ratios at relatively low concentrations of MgAc.
Nascent chains labelled with f[35S]Met were released at various concentrations of KCl, MgAc, and spermine in the lysate system. Release of the nascent chains was inhibited at low concentrations of KCl, but almost 90% of the nascent chains were released at 70-120mM KCl. The release of the nascent chains was maximum around optimum concentrations of MgAc, but inhibited at other concentrations. Spermine stimulated the release of the nascent chains. The analyses of the released chains showed that the ratio of f[35S]Met-α to f[35S]Met-β globin chains was almost constant at various concentrations of KCl, MgAc, and spermine.
Nascent chains were labelled with f[35S] Met at various concentrations of KCl, MgAc, and spermine. The labelled nascent chains were released in the incubation mixture under the optimum conditions. The analyses of the released chains showed that the ratio of f[35S]Met-α to f[35S]Met-β was similar to the α/β synthetic ratios obtained at various concentrations of KCl, MgAc, and spermine.
The data presented above suggest that changes in the α/β synthetic ratio with added KCl, MgAc, and spermine were due to changes in the rates of the translational initiation of α and β globin mRNAs.抄録全体を表示PDF形式でダウンロード (722K) -
Kanefusa KATO, Yoshitaka HAMAGUCHI, Sachiyo OKAWA, Eiji ISHIKAWA, Keik ...1977 年 82 巻 1 号 p. 261-266
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーRabbit antibody IgG was bound onto aminoalkylsilyl or plain glass rods by simple adsorption. For comparison, rabbit antibody IgG was also bound onto glutaraldehyde-activated aminoalkylsilyl glass rods. These antibody-glass rods were tested by the sandwich procedure using Fab' fragments of rabbit antibody conjugated with β-D-galactosidase from Escherichia coli. The glutaraldehyde-activated aminoalkylsilyl glass showed the largest capacity to bind antigen and the plain glass showed the smallest. However, the antibody-glass rods prepared by simple adsorption were as useful for the sandwich immunoassay of macromolecular antigens as those prepared with glutaraldehyde. With all the antibody-glass rods prepared, 0.1 to 10 fmol of ornithine δ-aminotransferase from rat liver and 2, 4-dinitrophenyl human IgG were measurable. More than 10 fmol of the antigens may be measurable with larger amounts of the antibody-β-D-galactosidase complexes, although the non-specific binding of the complexes to the solid phase increases to limit the sensitivity of the immunoassay.抄録全体を表示PDF形式でダウンロード (490K) -
Amino Acid SequencesToshiharu HASE, Keishiro WADA, Hiroshi MATSUBARA1977 年 82 巻 1 号 p. 267-276
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーTwo ferredoxins were isolated from horsetail (Equisetum telmateia) and their amino acid sequences were determined by use of a sequence analyzer in combination with carboxypeptidase digestion and manual Edman degradation of tryptic peptides of carboxymethyl-ferredoxins. Ferredoxins I and II each had only four cysteine residues in a total of 95 and 93 residues, respectively. The amino-terminal residues of both ferredoxins were heterogeneous, but alanine was concluded to be their genuine terminal residue. The comparison of these isozymelike molecules showed 29 differences in amino acid residues with three inverted replacements. One gap was inserted in ferredoxin II at position 32 to align the ferredoxins with greatest homology. Despite the many differences in amino acid residues there was no difference in net charges of the two ferredoxins.抄録全体を表示PDF形式でダウンロード (620K) -
Amino Acid Sequences and Gene DuplicationToshiharu HASE, Keishiro WADA, Hiroshi MATSUBARA1977 年 82 巻 1 号 p. 277-286
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーAmino acid sequences of two ferredoxins isolated from Equisetum arvense were determined by conventional procedures. Ferredoxins I and II of E. arvense had 95 and 93 residues, respectively, and nearly identical sequences each with only one amino acid difference from ferredoxins I and II of E. telmateia (1). The overall structural characteristics of these two ferredoxins were therefore very similar to those of E. telmateia ferredoxins. Ferredoxins I and II from E. arvense differ in 31 sites and those from E. telmateia in 29 sites from each other. These facts suggested that duplication of the ferredoxin gene in one organism occurred at an early evolutionary stage long before the divergence of the two horsetail species. The number of differences in amino acids between horsetail ferredoxins and other chloroplast-type ferredoxins indicated that the duplication occurred after divergence of horsetails from other plants. Comparing green plant ferredoxins, it was estimated that this gene duplication occurred about 250 million years ago. Some comments on the unique amino acid substitutions in horsetail ferredoxins are also presented.抄録全体を表示PDF形式でダウンロード (639K) -
Michio YAZAWA, Koichi YAGI1977 年 82 巻 1 号 p. 287-289
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーMyosin light chain kinase from frozen rabbit skeletal muscle was separated into two protein components by DEAE-cellulose column chromatography. One component (MW 20, 000) was a Ca2+-binding protein. Both components and Ca2+ were essential for the enzyme activity.抄録全体を表示PDF形式でダウンロード (189K) -
Hiroyuki KAGAMIYAMA, Ryuzo SAKAKIBARA, Hiroshi WADA, Sumio TANASE, Yos ...1977 年 82 巻 1 号 p. 291-294
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe complete amino acid sequence of the mitochondrial aspartate aminotransferase from pig heart was determined by analyses of the fragments obtained from tryptic digestion and cyanogen bromide treatment of the protein. The sequence analyzer was useful for establishing the primary structure of the N-terminal portion of the whole protein. There are 401 amino acid residues in the molecule. The sequence was compared with that of the cytoplasmic isozyme, showing 48% homology.抄録全体を表示PDF形式でダウンロード (231K) -
Yukio MITSUI, Yoshinori SATOW, Toshio SAKAMAKI, Yoichi IITAKA1977 年 82 巻 1 号 p. 295-298
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe crystal structure of a protein proteinase inhibitor, Streptomyces subtilisin inhibitor which strongly inhibits bacterial alkaline proteinases, was determined at 2.3 Å resolution. The subunit (molecular weight, 11, 485) of this dimeric molecule has a unique fold. of polypeptide chain with a five-fold anti-parallel β-sheet structure (about 21% of the 113 amino acid residues) and two small segments of α-helices (about 16%). The region around the apparent reactive site, Met(73)-Val(74), is held tight by a combination of various structural features. The conformation of this region seems to have close similarity to that found in substrate analogues of low molecular weight bound to subtilisin BPN'.抄録全体を表示PDF形式でダウンロード (234K) -
Mitsuo IKEBE, Hirofumi ONISHI, Shizuo WATANABE1977 年 82 巻 1 号 p. 299-302
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーChicken gizzard myosin was incubated with ATP and/or “native” tropomyosin (NTM) of gizzard muscle in the presence or absence of calcium ions. One of the two light chains of the myosin molecule was phosphorylated in the presence of Ca, but not in its absence. The phosphorylated gizzard myosin was dephosphorylated by a crude preparation of myosin lightchain phosphatase obtained from gizzard muscle.
In a superprecipitation test in the presence of EGTA, actomyosin reconstituted from dephosphorylated gizzard myosin did not superprecipitate, whereas actomyosin reconstituted from phosphorylated gizzard myosin showed superprecipitation activity which was inhibited by skeletal NTM and reactivated by Ca.抄録全体を表示PDF形式でダウンロード (896K) -
Difference between Crude Brain Extract and Purified Microtubular ProteinsEisuke NISHIDA, Hikoichi SAKAI1977 年 82 巻 1 号 p. 303-306
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーMicrotubule reassembly in crude extracts of porcine brain was inhibited by 10-5M Ca2+, whereas 10-3M Ca2+ was required for inhibition of the reassembly from purified microtubular proteins. This accounts for the apparent discrepancies between the results reported by other investigators. Furthermore, the Ca-sensitivity of the purified microtubular proteins was nearly completely recovered on addition of a fraction obtained from crude brain extract.抄録全体を表示PDF形式でダウンロード (241K) -
Yoshiko EGI, Takashi KAWASAKI1977 年 82 巻 1 号 p. 307-309
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーTo determine whether the acid phosphatase in Micrococcus denitrificans participates in hydrolysis of thiamine phosphate in the synthesis of thiamine pyrophosphate, acid phosphatase was purified 280-fold by conventional procedures, which removed thiamine phosphate phosphatase completely. Studies showed that this acid phosphatase is a different protein from thiamine phosphate phosphatase and that it has no binding site for thiamine phosphate on its active site.抄録全体を表示PDF形式でダウンロード (195K) -
Masatomo MAEDA, Hiroshi KOBAYASHI, Masamitsu FUTAI, Yasuhiro ANRAKU1977 年 82 巻 1 号 p. 311-314
発行日: 1977/07/25
公開日: 2008/11/18
ジャーナル フリーThe metabolic stabilities of bound adenine nucleotides in a membrane-bound ATPase (EF1) [EC 3. 6. 1. 3] of Escherichia coli were studied by estimating their rates of turnover in vivo. Two-thirds of the bound ATP prelabelled with 32P1 in EF1 molecules was retained after 3h in a chase medium. The bound ADP was chased rapidly with a half time of decrease of less than 1h, the rate being similar to that of cytoplasmic free nucleotides. These results suggest that bound ATP in the EF1 is not a direct intermediate in oxidative phosphorylation.抄録全体を表示PDF形式でダウンロード (286K) -
1977 年 82 巻 1 号 p. 315
発行日: 1977年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (50K)
- |<
- <
- 1
- >
- >|