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  • Iyuki Namekata, Ryosuke Odaka, Shogo Hamaguchi, Hikaru Tanaka
    2002 Volume 30 Pages 1-14
    Published: 2002
    Released on J-STAGE: February 04, 2023
    In this review, we provide an overview of our studies on the myocardial Na+-Ca2+ exchanger, an antiporter located on the sarcolemma that catalyzes the electrogenic exchange of three Na+ and one Ca2+, either in the Ca2+ efflux (forward) or Ca2+ influx (reverse) modes. Firstly, an inhibitor of the Na+-Ca2+ exchanger was evaluated by fluorescence microscopy and a kinetic analysis. Secondly, intracellular Ca2+ oscillations were observed by rapid scanning confocal microscopy and the role of the forward-mode Na+-Ca2+ exchanger in myocardial excitation was clarified. Thirdly, the involvement of the reverse-mode Na+-Ca2+ exchanger in cellular damage during myocardial ischemia and reperfusion was demonstrated with various fluorescent probes. Our research was supported by bioimaging technology in combination with electrophysiology and pharmacology. Bioimaging is a powerful methodology in modern biology. With some caution and effort to optimize the technology to meet research needs, bioimaging would be fruitful for all researchers.
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