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Volume 8, Issue 3
Displaying 1-4 of 4 articles from this issue
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Review
  • Actin Dynamics in Living Growth Cones as Revealed by the LC-Pol Scope
    Kaoru Katoh
    Article type: Review
    2000 Volume 8 Issue 3 Pages 83-90
    Published: 2000
    Released on J-STAGE: September 04, 2018
    DOIhttps://doi.org/10.11169/bioimages.8.83
    JOURNAL FREE ACCESS
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    A growth cone, an enlargement of the cytoplasm at the tip of A growing neurite, plays important roles in the navigation of neurites or path finding for neuronal elongation. Since the growth cone was first reported by Cajal, more than 100 years have passed. Nevertheless, the motile mechanism of the growth cone is still unclear. One of the reasons is the difficulty in observing the actin-based cytoskeletal structures of the living growth cones. We applied a new type of polarized microscope, the LC-pol scope, for the observation of filamentous actin in living growth cones. The LC-pol scope revealed the dynamics of filamentous actin in the living unstained growth cones, which are related to filopodial or lamellipodial movement.
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Regular Articles
  • A Convenient Method for Analyzing Differentially Expressing Genes in Plural Cells
    Takahiro Nemoto, Sachiko Kamei, Yousuke Seyama, Shunichiro Kubota
    Article type: Regular Article
    2000 Volume 8 Issue 3 Pages 91-96
    Published: 2000
    Released on J-STAGE: September 04, 2018
    DOIhttps://doi.org/10.11169/bioimages.8.91
    JOURNAL FREE ACCESS
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    Differential display PCR is an effective method to identify and isolate genes that are differentially expressed in various cells or under different conditions. To display differentially expressed PCR products, either a radioisotope or a fluorescence method is needed. For the latter method a very expensive fluorescence image analyzer is necessary. Here we report a convenient and inexpensive method using silver staining without the need for a fluorescence image analyzer to display differentially expressed genes. We successfully identified several interesting genes differentially expressed in COS cells and in ornithine decarboxylase (ODC) overexpressing COS cells. The results suggest that this modified new method is useful for identifying genes differentially expressed in different cells or in the same cells under different conditions.
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  • High Resolution Shape Analysis of Locomoting Cells using the Graded Radial Extension Model
    Takafumi Mizuno, Tatsuya Sakai, Kyoko Yoshioka, Kazunori Kawasaki
    Article type: Regular Article
    2000 Volume 8 Issue 3 Pages 97-104
    Published: 2000
    Released on J-STAGE: September 04, 2018
    DOIhttps://doi.org/10.11169/bioimages.8.97
    JOURNAL FREE ACCESS
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    The GRE (graded radial extension) model is the only kinematic model of cell locomotion that relates actin cytoskeleton dynamics to whole cell movement. To detect and quantify faint changes in cell shape and movement after various manipulations, we developed a new method for detailed shape analysis of locomoting fish keratocytes. The cells, loaded uniformly with a fluorescent cytoplasm-staining dye, were observed by a LSCM. Their motility and shape changes were analyzed by digital image processing. Outlines of cell margins were extracted from digitized microscope images, and the predicted next-frame outlines were calculated using the GRE model. Actual observed outlines of cell margins in the next frame were compared with the predicted ones, and difference plots were produced. Thus, local abnormalities along the cell margin were clearly detected and quantified. Using this method, we found a slight “swinging” cell body movement in the normal “sliding” mode of fan-shaped keratocyte locomotion.
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  • Quantitative Analysis of Cortical Actin Filaments in Dividing Sea Urchin Eggs
    Setsuko K. Satoh, Yukihisa Hamaguchi
    Article type: Regular Article
    2000 Volume 8 Issue 3 Pages 105-111
    Published: 2000
    Released on J-STAGE: September 04, 2018
    DOIhttps://doi.org/10.11169/bioimages.8.105
    JOURNAL FREE ACCESS
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    In sea urchin eggs, the quantitative analysis of cortical actin filaments was carried out during the first cleavage. At about midstage of cleavage, the actin amounts at the furrow became 2.5 times those of the polar cortex and then gradually decreased, which indicates that 60% or more of actin in the contractile ring was assembled during division. The width of the accumulation of actin filaments around the furrow also increased to 40 μm along the cortex at the midstage of cleavage and to 30 μm along the pole-to-pole axis, and then remained constant. In the dividing eggs treated with cytochalasin B, the furrow cortex was found to be patched l min after the treatment, which indicates that the contractile ring had broken. By 3 min after the treatment, the decrease rate of fluorescence at the furrow region had become remarkable and the actin amounts in the furrow were less than those in the rest of the cortex.
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