bioimages
Print ISSN : 0919-2719
Volume 9 , Issue 3+4
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Regular Article
  • Keiji Umetani, Satoshi Itasaka, Masakazu Ogura, Hiroyuki Kimura, Masah ...
    Type: Regular Article
    2001 Volume 9 Issue 3+4 Pages 97-106
    Published: 2001
    Released: June 07, 2017
    JOURNALS FREE ACCESS
    The development of new blood vessels (angiogenesis) is important in the pathogenesis of rapid growth of solid tumors. Microangiography with a spatial resolution down to 8 μm was carried out at SPring-8 for the depiction of angiogenic vessels in a mouse model of cancer using a high-resolution image detector and a third-generation synchrotron radiation source. The new image detector is a fluorescent-screen optical-lens coupling system using a high-sensitivity pickup-tube camera. X-rays are converted into a visible image in a phosphor screen layer, and a visible light image is detected by the pickup tube. After analog-to-digital conversion, images are stored in a digital frame memory system with a 1024 × 1024-pixel and 10-bit format. In synchrotron radiation radiography, a long source-to-object distance and a small source spot can produce high-resolution images. A double crystal monochromator selected a single synchrotron radiation energy for imaging. Murine tumors had been transplanted into mice dorsal skin. Using a 9.0-keV monochromatic X-ray beam, small tumor blood vessels with diameters of 15 − 20 μm in an immature vascular network produced by angiogenesis were visualized after an iodine contrast material was injected into the common carotid artery.
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