Journal of Bio-Integration
Online ISSN : 2186-2923
Volume 10, Issue 1
Displaying 1-4 of 4 articles from this issue
  • OTSUKA Yuta, Akira ITO, Masaki TAKEUCHI, Hideji TANAKA
    2020 Volume 10 Issue 1 Pages 2-12
    Published: 2020
    Released on J-STAGE: July 19, 2020
    JOURNAL OPEN ACCESS
    The purpose of thisreview is to clarify the rapid self setting reaction of apatite cement (SSAC) consisting of tetracalcium phosphate (TeCP) and dicalcium phosphate dihydrate (DCPD) by ombining attenuated total reflection Fourier transform infrared spectroscopy (ATR IR) and m ultivariate analysis . The ATR IR spectra of the sample showed characteristic phosphoric acid peak due to crystalline transformation from TeCP and DCPD into hydroxyapatite (HAp). M ultivariate analysis result s indicated that HA p wa s formed by precipitation and re dissolution of T e CP and DCPD in the samples. In the case of HAp crystallization with amino acids , serine and phospho serine were adsorbed onto HAp seed crystals and inhibit their crystallization was indicated. It is concluded that the It is concluded that the electrostatic interaction between seed HAp crystal and amino acids has to be considered for developing drug delivery system.
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  • Ai YAMAMOTO, Kazuhiro KON, Maiko YAMAMOTO, Hiroshi KOBAYASHI, Seiji OH ...
    2020 Volume 10 Issue 1 Pages 13-23
    Published: 2020
    Released on J-STAGE: July 19, 2020
    JOURNAL FREE ACCESS
    Purpose: Lateral sinus floor augmentation is commonly performed as a predictable procedure optimizing the placement of dental implants into atrophied maxillary alveolar ridge. It has been reported that implant survival rates with lateral sinus floor augmentation is 98%. However, for a precise diagnosis and screening prior to surgery, understanding the systemic or local risk factors may affect clinical outcomes. The aim of this study was to evaluate effects of the various factors of lateral sinus floor augmentation on implant survival rate. Materials and Methods: A total of 398 patients treated with a combination of lateral sinus floor augmentation and dental implant placement at Dental Hospital of Tokyo Medical and Dental University from 2000 to 2014 were examined. We evaluated the effects of the following factors on implant survival rate: Diabetes,smoking,nasal disease,respiratory disease with steroid drug,residual bone height and surgical procedure, implant system, bone substitute, sinus membrane perforation,cover screw exposure and periodontitis.The differences of the means were analyzed by logistic regression analysis. Results: The number of implants could not acquire osseointegration was 43 in total, and the survival rate was 94.3%. Nasal disease (p=0.00856), implant system (p=0.00043) and cover screw exposure (p=0.0390) were related to implant failure. Discussions and conclusions: The present results indicate that nasal disease and postoperative cover screw exposure affected implant survival rate. Corroboration with an otolaryngologist, treating nasal disease before maxillary sinus floor augmentation and avoiding postoperative cover screw exposure are important.
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  • Motoi MIURA, Hidemi NAKATA, Shohei KASUGAI, Shinji KURODA
    2020 Volume 10 Issue 1 Pages 24-46
    Published: 2020
    Released on J-STAGE: July 19, 2020
    JOURNAL OPEN ACCESS
    Objective: Periosteum is considered to be involved in maintenance and regeneration of the bone and gingiva. Here, we investigated whether periosteum-derived cells (PDCs) display the ability of gingival fibroblast differentiation and osteoblast differentiation, and examined their characteristics of fibrogenesis compared with that of bone marrow cells (BMCs). Methods: PDCs and BMCs were prepared from skull periosteum and bone marrow of femurs in 4-week-old female ICR mice and cultured in growth medium (D) supplemented with fibrogenic (F) or osteogeneic (O) medium for 3 weeks, followed by additional subtreatments with F or O for 2 weeks. Cells were also cultured with SIS3, a Smad3 phosphorylation inhibitor, followed by TGF-β treatment. Gene and protein expressions related to fibrogenic and osteogenic differentiation were evaluated with RT-PCR and western blot and immunostaining, respectively. Results: Both cells showed ALP activity after osteogenic induction, with PDCs showing increased ALP activity by sub-treatment of D-O and F-O, but not with BMCs. PDCs showed higher gene expression of Runx2, Fgfr1, and α-SMA compared with BMCs in osteogenic culture and after sub-treatment with F. Furthermore, Fgfr1 gene expression was stronger in PDCs than in BMCs after inhibiting Smad3 phosphorylation. Notably, TGF-β treatment stimulated or recovered the expression of S100A4 and vimentin, even after inhibition of Smad3 phosphorylation in BMCs, but not in PDCs. Conclusion: PDCs might have potential of both osteogenic and fibrogenic differentiation. Furthermore, TGF-β signaling could occur without Smad3 phosphorylation in PDCs, indicating presence of a non-canonical signal transcription factor in PDCs.
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  • Wai Myo Maung, Hidemi Nakata, Munemitsu Miyasaka, You-kyoung Kim, Moto ...
    2020 Volume 10 Issue 1 Pages 47-60
    Published: 2020
    Released on J-STAGE: July 19, 2020
    JOURNAL OPEN ACCESS
    The replacement of lost natural teeth using osseointegrated implants require adequate bone volume permitting their anchorage. The periosteum is a specialized fibrous tissue composed of fibroblast, osteoblast, and progenitor cells and optimal source for bone tissue engineering based on its accessibility and rapid proliferation in vivo and in vitro. Low-intensity pulsed ultrasound (LIPUS) has shown successful bone healing of delayed, non-unions and fracture healing in animal models and in clinical treatments. This study aimed to investigate the effects of LIPUS on the proliferation and osteogenic differentiation of murine calvarial periosteum-derived cells (PDCs). PDCs were treated with daily ultrasound stimulation with an intensity of 30 mW/cm2, frequency of 3 MHz, pulse repetition frequency (PRF) of 1 kHz and exposure time of 20 min per day up to 21 days. The cell proliferation and viability were analyzed with PrestoBlue Cell Viability assay. Osteogenic differentiation was determined with alkaline phosphatase (ALP) staining, ALP activity assay, alizarin red staining, and real-time reverse transcriptase- polymerase chain reaction. The results indicated that LIPUS stimulation did not significantly affect the viability of PDCs. LIPUS significantly increased ALP activity on day 7 of culture and markedly promoted the formation of mineralized nodules on day 21. The mRNA expression of ALP, RUNX2, OSX, and OCN was significantly up-regulated by LIPUS stimulation. Thus, LIPUS stimulation increases osteogenic differentiation of PDCs and is useful for potential bone regeneration.
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